ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII0586  suspect: LH   U     KOG4324  Intracellular trafficking, secretion, and vesicular transport  Guanine nucleotide exchange factor

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0586  196243 194270 -658 
         (658 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YNL272c [U] KOG4324 Guanine nucleotide exchange factor                450   e-126
SPAC23C4.10 [U] KOG4324 Guanine nucleotide exchange factor             57   9e-08

>YNL272c [U] KOG4324 Guanine nucleotide exchange factor
          Length = 759

 Score =  450 bits (1158), Expect = e-126
 Identities = 260/702 (37%), Positives = 394/702 (56%), Gaps = 47/702 (6%)

Query: 1   MADQEESKRISLQVTSLSTQLIESIDKQSQLEEQLLVAKKTISTQQNIANSYNXXXXXXX 60
           M   EE+KR+S+QVTSLSTQLIES+DKQS LEEQL  + KTI++Q+    +YN       
Sbjct: 1   MDASEEAKRVSIQVTSLSTQLIESVDKQSHLEEQLNKSLKTIASQKAAIENYNQLKEDYN 60

Query: 61  XXXXXYETDKTKFK-------KQEEICADAEKKVATLNQEIEDLTASLFDEANNMVADAR 113
                      + K       K+ E+   AE++   LN+E+EDLTASLFDEANNMVADAR
Sbjct: 61  TLKRELSDRDDEVKRLREDIAKENELRTKAEEEADKLNKEVEDLTASLFDEANNMVADAR 120

Query: 114 KEQNAVEILNSXXXXXXXXXXXXXXXXSMQLKNLKNVLYKFENDFNSSKNVXXXXXXXXX 173
           KE+ A+EILN                 ++QLKNLK V++  +N+   + N          
Sbjct: 121 KEKYAIEILNKRLTEQLREKDTLLDTLTLQLKNLKKVMHSLDNESTVTNNSNRYSTILSD 180

Query: 174 XXXXXXXXXNLTSVNDNSLS---FQGVLFSPLLHSLRYDVPLYTEYLKFLAVLPQCENLQ 230
                    N    + +  S   + G+++SP + S+RYD+ LY E+LKF+A LP+CEN++
Sbjct: 181 SATSSSTSLNKVPTSYSLASQDIYSGIVYSPSISSIRYDISLYNEFLKFVAALPRCENIK 240

Query: 231 QTTSESKLLRRLINYEISPVLRLDNASGVGWLVKRNLMNLMMEGLVSIEPISGINETYRT 290
            T++ESKL+RRL+N EI P+L++DNASG+GWLVK+ L++L+++GLV +EP+SG+N TY+ 
Sbjct: 241 ATSTESKLIRRLVNDEIQPILKIDNASGIGWLVKKTLLSLIIDGLVVVEPLSGVNATYQI 300

Query: 291 GYSSPSIPDSPEVENKDSHLFSRPVNSPPVAMVEPCAFCGENRNDILEHGRLYIFKTYQK 350
           GY+S S   + +  +    +F  P++SPPVA+   C+FCGE+R+DI+EH R+YI KT  K
Sbjct: 301 GYNSSS--PAKQATSNMPKMFKFPLDSPPVAVHAACSFCGESRDDIIEHARMYILKTLHK 358

Query: 351 LENGKNELTNQFPVCHYCLLKLRQVCEIFAFLRSLRSGAWHLEKVALASIHQGKVNAYSE 410
            ++GK ++TN +P+CH+CLLKLRQ CEIFAFLRSL+ GAWHLEK+   +I +  +  +SE
Sbjct: 359 TDDGKEQVTNTYPLCHWCLLKLRQTCEIFAFLRSLKVGAWHLEKLTTQNITKEDLEKFSE 418

Query: 411 VTK-------ASRPKMKSKRLSIFQGL--KQSQSSTPIIE-NGPQFADKNGLPTTNIQRS 460
           VTK        S    K+KRLS   GL    S  + P +E    +   K G PTTNIQR+
Sbjct: 419 VTKHTKRDGRVSSQDKKTKRLSFMAGLGINSSTKNKPKMEIFSSETNAKPGQPTTNIQRA 478

Query: 461 WARLSQLRASLQWSHIGVWSLDESVATDIAPSTDISKAKSFEEE-----QGLGIASNNIP 515
           W +L +LR  L W+HIG+W++D+S+++ I P  +    +   +      Q   +   +  
Sbjct: 479 WLQLCKLRCILHWTHIGIWAVDDSISSKIGPLVEDDSDEDQNDAISVRLQDKALWKQDAK 538

Query: 516 VPITHXXX-XXXXXXXXXXNSISVDETVNGIRHTDQSSDDIGDHNEKTNEKTATDLQGKN 574
            P +                S  ++  + G   +D+SS D    +  T + ++ D     
Sbjct: 539 RPFSSSSAEESQKSDAFDFESGDMENEITGESSSDESSSDGSSTDNSTADSSSEDESSLA 598

Query: 575 EEKKSK--KIEPQHSRQPSQSSTVLNDNKESAESAHDTHASSE-------------EPSD 619
           +   S      P+         TV  D+K S +SA++   +S+              P  
Sbjct: 599 DSTTSSADSSSPESIDNGEGDDTVTKDDKSSIKSANNNEENSDCGDKKGRSIIKKKAPQR 658

Query: 620 LLE---TYQDLSADLEPESEKPENLDNSDEEDSFNDAKDVVN 658
            ++     QDL  DLE +  +   +D ++ E++ ++ K  ++
Sbjct: 659 KIQKKKLLQDLD-DLEEQFREESAIDQTEFENAESNVKQNIS 699


>SPAC23C4.10 [U] KOG4324 Guanine nucleotide exchange factor
          Length = 527

 Score = 57.0 bits (136), Expect = 9e-08
 Identities = 39/157 (24%), Positives = 73/157 (45%), Gaps = 3/157 (1%)

Query: 3   DQEESKRISLQVTSLSTQLIESIDKQSQLEEQLLVA---KKTISTQQNIANSYNXXXXXX 59
           ++E + ++  Q   LS +L+ +I++Q+ L+++        + +S +     +        
Sbjct: 17  EKEVNGKLMSQEEILSQKLVAAIERQAALDDKYATEVHRNEALSERIRELEAKLSAKKSF 76

Query: 60  XXXXXXYETDKTKFKKQEEICADAEKKVATLNQEIEDLTASLFDEANNMVADARKEQNAV 119
                 +   + K +  E  C +AE + + +  E+EDLT+SLF+EAN MVA+ARKE  A 
Sbjct: 77  DEELVKFHNLEEKLQLTETKCRNAESEKSRVENELEDLTSSLFEEANRMVANARKETVAS 136

Query: 120 EILNSXXXXXXXXXXXXXXXXSMQLKNLKNVLYKFEN 156
           E   +                  QL  LK+V++   +
Sbjct: 137 EKRVNQLKKQLVDAETLLSSTQHQLTELKDVMHSMSD 173


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.309    0.127    0.347 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,084,092
Number of Sequences: 60738
Number of extensions: 1452185
Number of successful extensions: 6366
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6356
Number of HSP's gapped (non-prelim): 4
length of query: 658
length of database: 30,389,216
effective HSP length: 113
effective length of query: 545
effective length of database: 23,525,822
effective search space: 12821572990
effective search space used: 12821572990
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)