ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0679 good S KOG3094 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0679 228731 227817 -305
(305 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNL263c [S] KOG3094 Predicted membrane protein 266 4e-71
SPBC25H2.06c [S] KOG3094 Predicted membrane protein 109 4e-24
7301494 [S] KOG3094 Predicted membrane protein 100 3e-21
Hs20545322 [S] KOG3094 Predicted membrane protein 85 1e-16
At1g30890 [S] KOG3094 Predicted membrane protein 84 2e-16
At3g59500 [S] KOG3094 Predicted membrane protein 81 2e-15
CE05983 [S] KOG3094 Predicted membrane protein 75 1e-13
Hs22062544 [S] KOG3094 Predicted membrane protein 71 2e-12
Hs9994169 [S] KOG3094 Predicted membrane protein 69 1e-11
ECU09g1590 [S] KOG3094 Predicted membrane protein 66 5e-11
>YNL263c [S] KOG3094 Predicted membrane protein
Length = 314
Score = 266 bits (679), Expect = 4e-71
Identities = 145/252 (57%), Positives = 171/252 (67%), Gaps = 11/252 (4%)
Query: 63 NMAFQFGQTAFNQFVGQENISQFQETMSKATG--ATNSSVAHYFQVSNSYVFHKFKLITV 120
+MAFQ GQ+AF+ F+GQ+N +QFQET++KAT A + ++ YFQVS YV +K KLI V
Sbjct: 65 SMAFQLGQSAFSNFIGQDNFNQFQETVNKATANAAGSQQISTYFQVSTRYVINKLKLILV 124
Query: 121 PFLQ--KNWQRIPDSSNSFQPPRIDVNSPDLYIPVMGLVTYILAWNVTQGLNGSFDPENL 178
PFL KNWQRI DS N F PPR DVNSPD+Y+P+MGLVTYIL WN QGL GSF+PE+L
Sbjct: 125 PFLNGTKNWQRIMDSGN-FLPPRDDVNSPDMYMPIMGLVTYILIWNTQQGLKGSFNPEDL 183
Query: 179 YFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTSLVELTCYVGYKFVPLIFSMFFP--A 236
Y+KLSST + ++ P SLVEL CYVGYKFVPLI +
Sbjct: 184 YYKLSSTLAFVCLDLLILKLGLYLLI-DSKIPSFSLVELLCYVGYKFVPLILAQLLTNVT 242
Query: 237 SFIWLNRLAKLYLFVAFGVFLLRSIKFNLFADMGNSAD---FSFKKGTVKKVNYFLFVYG 293
LN L K YLF+AFGVFLLRS+KFNL + G D S K TVKK NYFLFVYG
Sbjct: 243 MPFNLNILIKFYLFIAFGVFLLRSVKFNLLSRSGAEDDDIHVSISKSTVKKCNYFLFVYG 302
Query: 294 FFWQSVLMWLMG 305
F WQ+VLMWLMG
Sbjct: 303 FIWQNVLMWLMG 314
>SPBC25H2.06c [S] KOG3094 Predicted membrane protein
Length = 293
Score = 109 bits (273), Expect = 4e-24
Identities = 80/245 (32%), Positives = 121/245 (48%), Gaps = 20/245 (8%)
Query: 55 SYLP-SASTNMAFQFGQTAFNQFVGQENISQ-FQETMSKATGATNSSVAHYFQVSNSYVF 112
+YLP SA+ M FQ G+ A N GQE + Q F + +S + + HYF V+NSYV
Sbjct: 46 AYLPNSATAQMGFQLGKNAVN--AGQEYVEQNFGKWLS------TTRLHHYFTVTNSYVV 97
Query: 113 HKFKLITVPFLQKNWQR------IPDSSNSFQPPRIDVNSPDLYIPVMGLVTYILAWNVT 166
K LI P+ +++W R I S+ + PP D+NSPD+YIP+M T+IL
Sbjct: 98 AKLLLIIFPWRRRSWARKLRRSEINGSAEGYCPPAEDLNSPDMYIPLMAFTTHILLLCAL 157
Query: 167 QGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTSLVELTCYVGYKFV 226
GL F PE F L ++ + N +S + +++L + GYKFV
Sbjct: 158 AGLQDDFQPE--LFGLRASKACAVVLVEFLATRLGCYLLNISSQ-SQVLDLLAFSGYKFV 214
Query: 227 PLIF-SMFFPASFIWLNRLAKLYLFVAFGVFLLRSIKFNLFADMGNSADFSFKKGTVKKV 285
LI S+ W+ R LY+++A FLLRS+K+ + + + + + +
Sbjct: 215 GLILTSLSKLFEMPWVTRFVFLYMYLATAFFLLRSLKYAVLPESTMAINATITSHQRSRR 274
Query: 286 NYFLF 290
YFLF
Sbjct: 275 IYFLF 279
>7301494 [S] KOG3094 Predicted membrane protein
Length = 397
Score = 100 bits (249), Expect = 3e-21
Identities = 72/249 (28%), Positives = 121/249 (47%), Gaps = 37/249 (14%)
Query: 63 NMAFQFGQTAFNQFVGQENI-SQFQETMSKATGATNSSVAHYFQVSNSYVFHKFKLITVP 121
+MA Q+GQ +Q G++ + +QF++ + A + +YF V N+YV K +L+ P
Sbjct: 136 DMAMQYGQKLADQ--GKQIMENQFEKWVPVA------KLKYYFAVDNAYVGRKLRLLFFP 187
Query: 122 FLQKNWQRIPDSSNSFQPPRIDVNSPDLYIPVMGLVTYILAWNVTQGLNGSFDPENLYFK 181
++ K+W D + QP R DVN+PDLY+P MG +TY++ + G+ F PE L +
Sbjct: 188 YMHKDWSLRYDQEHPVQP-RYDVNAPDLYLPTMGYITYVIVAGLLLGMQKRFSPEQLGIQ 246
Query: 182 LSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTSLVELTCYVGYKFVPLIF-----SMFFPA 236
SS V N + + +L +L + GYK+V ++ ++FF +
Sbjct: 247 ASS--AMAYSIFELVIYSLALYVMNVKTSLKTL-DLLAFTGYKYVNIVVCLMVSTLFFKS 303
Query: 237 SFIWLNRLAKLYLFVAFGVFLLRSIKFNLFAD--------------MGNSADFSFKKGTV 282
+ +A Y +FG F+LR+++ L D GN F + G
Sbjct: 304 GYY----IALAYTSFSFGFFMLRTLRTKLLQDNSPAAPSGAINYDPYGNPQQFDYSGGKK 359
Query: 283 KKVNYFLFV 291
+K+ YFLF+
Sbjct: 360 RKL-YFLFM 367
>Hs20545322 [S] KOG3094 Predicted membrane protein
Length = 317
Score = 84.7 bits (208), Expect = 1e-16
Identities = 61/242 (25%), Positives = 104/242 (42%), Gaps = 12/242 (4%)
Query: 28 SATGKQYGMXXXXXXXXXXXXXXXXNGSYLPSASTNMAFQFGQTAFNQFVGQENISQFQE 87
SA + YG + ++L +NMA +G + Q G+E + + +
Sbjct: 47 SAQSRGYGAQRAPGGLSYPAASPTPHAAFLADPVSNMAMAYGSSLAAQ--GKELVDKNID 104
Query: 88 TMSKATGATNSSVAHYFQVSNSYVFHKFKLITVPFLQKNWQRIPDSSNSFQPPRIDVNSP 147
T + +YF V YV K L+ P+L ++W+ + ++ PR DVN+P
Sbjct: 105 RFIPIT-----KLKYYFAVDTMYVGRKLGLLFFPYLHQDWE-VQYQQDTPVAPRFDVNAP 158
Query: 148 DLYIPVMGLVTYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNT 207
DLYIP M +TY+L + G F P+ L + SS NT
Sbjct: 159 DLYIPAMAFITYVLVAGLALGTQDRFSPDLLGLQASSALAWLTLEVLAILLSLYLVTVNT 218
Query: 208 TSPVTSLVELTCYVGYKFVPLIFSMFFPASFIWLNRLAKL-YLFVAFGVFLLRSIKFNLF 266
+ ++L ++GYK+V +I + F + L + VA VF++R+++ +
Sbjct: 219 D---LTTIDLVAFLGYKYVGMIGGVLMGLLFGKIGYYLVLGWCCVAIFVFMIRTLRLKIL 275
Query: 267 AD 268
AD
Sbjct: 276 AD 277
>At1g30890 [S] KOG3094 Predicted membrane protein
Length = 272
Score = 84.3 bits (207), Expect = 2e-16
Identities = 65/212 (30%), Positives = 103/212 (47%), Gaps = 20/212 (9%)
Query: 98 SSVAHYFQVSNSYVFHKFKLITVPFLQK-NWQRIPDSSN---SFQPPRIDVNSPDLYIPV 153
S +YFQV++ YV +K K++ PFL + +W RI + S++PP D+N+PDLYIP
Sbjct: 66 SDPQYYFQVNDQYVRNKLKVVLFPFLHRGHWTRISEPVGGRLSYKPPIYDINAPDLYIPF 125
Query: 154 MGLVTYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTS 213
M TY++ ++ GLNG F PE L + L + + +P
Sbjct: 126 MAFGTYVVLAGLSLGLNGKFTPEALNW-LFVKGLVGWFLQVMLLKVTLLSLGSGEAP--- 181
Query: 214 LVELTCYVGYKFVPLIFSMFFPASFIWLNRLAKL--YLFVAFGVFLLRSIKFNLFADMGN 271
L+++ Y GY F L + F A +W L + + G+FL++++K LFA++
Sbjct: 182 LLDIVAYGGYAFAGLCLAGF--AKIMWGYSYYALMPWTCLCTGIFLVKTMKRVLFAEV-R 238
Query: 272 SADFSFKKGTVKKVNYFLFVYGFFWQSVLMWL 303
S D S K +Y L +L+WL
Sbjct: 239 SYDSS-------KHHYLLLFLALVQFPLLIWL 263
>At3g59500 [S] KOG3094 Predicted membrane protein
Length = 259
Score = 81.3 bits (199), Expect = 2e-15
Identities = 63/212 (29%), Positives = 102/212 (47%), Gaps = 20/212 (9%)
Query: 98 SSVAHYFQVSNSYVFHKFKLITVPFLQK-NWQRIPDSSN---SFQPPRIDVNSPDLYIPV 153
S +YFQV++ YV +K K++ +PFL + +W RI + S++PP D+N+PDLYIP+
Sbjct: 53 SDPQYYFQVNDQYVRNKLKIVLLPFLHRGHWTRISEPVGGRLSYKPPIYDINAPDLYIPL 112
Query: 154 MGLVTYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTS 213
M TY++ ++ GL G F PE L + L + + +P
Sbjct: 113 MAFGTYVVLAGLSLGLYGKFTPEALNW-LFVKGMVGWFLQVMLLKITLLSLGSGEAP--- 168
Query: 214 LVELTCYVGYKFVPLIFSMFFPASFIWLNRLAKL--YLFVAFGVFLLRSIKFNLFADMGN 271
L+++ Y GY F L ++ IW L + + GVFL++++K LFA+
Sbjct: 169 LLDIVAYAGYTFTGLCLAVL--GKIIWGYSYYVLIPWTCLCTGVFLVKTMKRVLFAE-SR 225
Query: 272 SADFSFKKGTVKKVNYFLFVYGFFWQSVLMWL 303
S D S + +Y L +L+WL
Sbjct: 226 SYDSS-------RHHYLLIFVALAQFPLLIWL 250
>CE05983 [S] KOG3094 Predicted membrane protein
Length = 251
Score = 75.1 bits (183), Expect = 1e-13
Identities = 60/178 (33%), Positives = 76/178 (41%), Gaps = 21/178 (11%)
Query: 6 YYSEIGSDSNVGMAAAGKGKNISATGKQYGMXXXXXXXXXXXXXXXXNGSYLPSA----- 60
YYS+ SN G G+ S T QYG G + P
Sbjct: 44 YYSQ--QSSNQGFDGYGQQ---SPTQNQYGGYGQQQQQQQSYGQNNGFGGFQPQQLMSDP 98
Query: 61 STNMAFQFGQTAFNQFVGQENISQFQETMSKATGATNSSVAHYFQVSNSYVFHKFKLITV 120
N A QFG QF Q+ +E ++K G N + +YF V N+YV K ++
Sbjct: 99 MLNAAKQFG----GQFAEQQ-----KEKLTKYLGTFN--LKYYFAVDNAYVGKKLGILFF 147
Query: 121 PFLQKNWQRIPDSSNSFQPPRIDVNSPDLYIPVMGLVTYILAWNVTQGLNGSFDPENL 178
PF K+W S P R DVN+PDLYIP+M +TYIL G G F PE L
Sbjct: 148 PFFHKDWSLKFAGSADPAPAREDVNAPDLYIPLMSFLTYILVSGFVLGTQGRFSPEIL 205
>Hs22062544 [S] KOG3094 Predicted membrane protein
Length = 293
Score = 70.9 bits (172), Expect = 2e-12
Identities = 42/135 (31%), Positives = 67/135 (49%), Gaps = 4/135 (2%)
Query: 98 SSVAHYFQVSNSYVFHKFKLITVPFLQKNWQRIPDSSNSFQPPRIDVNSPDLYIPVMGLV 157
S + ++F V +YV K L+ P+ +NW+ + S ++ PPR D+N+PDLYIP M +
Sbjct: 89 SKLKYFFAVDTAYVAKKLGLLVFPYTHQNWE-VQYSRDAPLPPRQDLNAPDLYIPTMAFI 147
Query: 158 TYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTSLVEL 217
TY+L + G+ F PE L L ++ ++ S + S L
Sbjct: 148 TYVLLAGMALGIQKRFSPEVL--GLCASTALVWVVMEVLALLLGLYLATVRSDL-STFHL 204
Query: 218 TCYVGYKFVPLIFSM 232
Y GYK+V +I S+
Sbjct: 205 LAYSGYKYVGMILSV 219
>Hs9994169 [S] KOG3094 Predicted membrane protein
Length = 293
Score = 68.6 bits (166), Expect = 1e-11
Identities = 41/135 (30%), Positives = 66/135 (48%), Gaps = 4/135 (2%)
Query: 98 SSVAHYFQVSNSYVFHKFKLITVPFLQKNWQRIPDSSNSFQPPRIDVNSPDLYIPVMGLV 157
S + ++F V +YV K L+ P+ +NW+ + S ++ PPR D+N+PDLYIP +
Sbjct: 89 SKLKYFFAVDTAYVAKKLGLLVFPYTHQNWE-VQYSRDAPLPPRQDLNAPDLYIPTKAFI 147
Query: 158 TYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXXXXXXXXXXVSNTTSPVTSLVEL 217
TY+L + G+ F PE L L ++ ++ S + S L
Sbjct: 148 TYVLLAGMALGIQKRFSPEVL--GLCASTALVWVVMEVLALLLGLYLATVRSDL-STFHL 204
Query: 218 TCYVGYKFVPLIFSM 232
Y GYK+V +I S+
Sbjct: 205 LAYSGYKYVGMILSV 219
>ECU09g1590 [S] KOG3094 Predicted membrane protein
Length = 206
Score = 66.2 bits (160), Expect = 5e-11
Identities = 48/187 (25%), Positives = 78/187 (41%), Gaps = 18/187 (9%)
Query: 77 VGQENISQFQETMSKATGATN-SSVAHYFQVSNSYVFHKFKLITVPFLQKNWQRIPDSSN 135
+G+E I + E S+ G + YF + N++V K LI PF K W +
Sbjct: 5 IGKEAIRKSTEYASRGLGGVSLKPFRTYFDIDNTFVLKKLVLILFPFNNKEWTGDDEG-- 62
Query: 136 SFQPPRIDVNSPDLYIPVMGLVTYILAWNVTQGLNGSFDPENLYFKLSSTXXXXXXXXXX 195
+ P+LY+P M ++YIL + GL G F PE L +
Sbjct: 63 --------MARPELYVPAMSFISYILLRALYLGLEGMFSPERLGIVFTRLFFLEAVCIAL 114
Query: 196 XXXXXXXXVSNTTSPVTSLVELTCYVGYKFVPLIFSMFFPASFIWLNRLAKLYLFVAFGV 255
S +++ Y GYK+V ++ ++ + + +YL+V+F V
Sbjct: 115 TRISGYF-----VDVGLSTLDVVAYSGYKYVIVLLLQLNKMRYVQV--IGGMYLYVSFFV 167
Query: 256 FLLRSIK 262
FL RS+K
Sbjct: 168 FLSRSLK 174
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.136 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,928,936
Number of Sequences: 60738
Number of extensions: 581168
Number of successful extensions: 1271
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1244
Number of HSP's gapped (non-prelim): 11
length of query: 305
length of database: 30,389,216
effective HSP length: 106
effective length of query: 199
effective length of database: 23,950,988
effective search space: 4766246612
effective search space used: 4766246612
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)