ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII0708  suspect: LH   R     KOG2950  General function prediction only                               Uncharacterized protein involved in protein-protein interaction, contains polyproline-binding GYF domain

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0708  237655  238629 325  
         (325 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YHR156c [R] KOG2950 Uncharacterized protein involved in protein-...   129   6e-30

>YHR156c [R] KOG2950 Uncharacterized protein involved in protein-protein
           interaction contains polyproline-binding GYF domain
          Length = 340

 Score =  129 bits (324), Expect = 6e-30
 Identities = 71/226 (31%), Positives = 123/226 (54%), Gaps = 10/226 (4%)

Query: 104 VQIEAFNMEEELKNGTVDKVGN--ATGKTINDDFENEDQWIQDFSSKESIKRAREAHENS 161
           V IE FN+++E+K+G  DK GN   T     +D ++ ++W+ D  + E + R  +  E S
Sbjct: 121 VNIEPFNIDDEIKHGVFDKDGNYIKTENATENDQQDNEEWMNDVINTEEVNRLEK--EQS 178

Query: 162 QTLQRKKRARMLYRLEEVLEQLYYF-VPRGKTIQEALILLQQLRKSLPKEHKERTKYIAN 220
              Q  +     Y + E L  L +F V   +T+ E+L  L +LRK    +  +  KY+ +
Sbjct: 179 VKTQNSRH----YMVHEALNLLKFFLVDENETVLESLGRLNKLRKIAISKKNKSLKYVIH 234

Query: 221 AIQIILTLVNVLEQKGIDNPMQLQKEGIRELYDEENLMGST-IDDVESKNWVFKWFADFE 279
            I+++  L+N+LE+KG     +  +  +++  +EE    S+ I + ++K W FKW    +
Sbjct: 235 GIELLSDLINILEKKGFSEVYEYNRLKVQDAIEEEIFDDSSRIVNHKTKLWGFKWLNKLD 294

Query: 280 TAHESFSNYEMQTWKEKYFNSSVVVKRKCDTDETDHWYHVDCIYFM 325
             H  ++NYEM  W++ YF +SV+VK   + D  ++W HV C+ FM
Sbjct: 295 EYHGLYTNYEMSYWQKSYFKNSVIVKFHSEPDRDENWIHVSCLSFM 340


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.314    0.131    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,283,673
Number of Sequences: 60738
Number of extensions: 673772
Number of successful extensions: 1927
Number of sequences better than 1.0e-05: 1
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1924
Number of HSP's gapped (non-prelim): 1
length of query: 325
length of database: 30,389,216
effective HSP length: 107
effective length of query: 218
effective length of database: 23,890,250
effective search space: 5208074500
effective search space used: 5208074500
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)