ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII0795  suspect: LH   K     KOG4183  Transcription                                                  RNA polymerase I 49 kDa subunit

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII0795  268209 266974 -412 
         (412 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YNL248c [K] KOG4183 RNA polymerase I 49 kDa subunit                   530   e-150
SPAC2F3.03c [K] KOG4183 RNA polymerase I 49 kDa subunit               159   7e-39

>YNL248c [K] KOG4183 RNA polymerase I 49 kDa subunit
          Length = 415

 Score =  530 bits (1366), Expect = e-150
 Identities = 267/415 (64%), Positives = 327/415 (78%), Gaps = 3/415 (0%)

Query: 1   MGSKRAFTEVGVSAYHDEPSVAVSSFFKGFQAPKDTTFELYKNKKNS-DKYVLHGENDRL 59
           M  KR+ +E+ + +  D+PSVAV SFFKGF+AP DTTF+LYK KK+  D++VLHGEN+RL
Sbjct: 1   MSVKRSVSEIEIESVQDQPSVAVGSFFKGFRAPSDTTFDLYKKKKSEKDEFVLHGENERL 60

Query: 60  EYEGQTDDDFVNSSQYVVGVYDAATKSVELYKAPXXXXXXXXXXRKNLMGPDIK-QANVR 118
           EYEG TD     S+QYVVG+++   KS++LYKAP           KNL GP IK +++ R
Sbjct: 61  EYEGYTDSSSQASNQYVVGLFNPEKKSIQLYKAPVLVSKVVSKSSKNLRGPKIKSKSDTR 120

Query: 119 AALMRNALGEAFGTKKAKKAIADMERNRIDSDKLVDSAIDIVDTVKTASKELPSKEELQE 178
            + +RNALGEAFGTKKAKKAIAD+ERNRIDSDKL DSAIDIVD+V+TASK+LP++ +L E
Sbjct: 121 PSALRNALGEAFGTKKAKKAIADLERNRIDSDKLTDSAIDIVDSVRTASKDLPTRAQLDE 180

Query: 179 TVSSDRPTPLADLDATDVEQIYPLYNIIPKKEWSFIRVGSIMKEQDPEKRLEYFPY-TKF 237
             S+DRPTPLA++DATDVEQIYP+ +IIPKKE  FIRV SI+KE D EK+LE FPY    
Sbjct: 181 ITSNDRPTPLANIDATDVEQIYPIESIIPKKELQFIRVSSILKEADKEKKLELFPYQNNS 240

Query: 238 QYVANKLPTXXXXXXXXXXXXXXXXXXXXXXXDNRRVNNKTKLLERINNPPDALLNGILE 297
           +YVA KL +                       +NRRVNNKTKLLER+N+PP+ L++GIL 
Sbjct: 241 KYVAKKLDSLTQPSQMTKLQLLYYLSLLLGVYENRRVNNKTKLLERLNSPPEILVDGILS 300

Query: 298 RFTISRPGQFGRSKDKSFFIDPQREDKLLCYILVIIMHLDNFIVEVPPLAQQLNLKPSKL 357
           RFT+ +PGQFGRSKD+S+FIDPQ EDK+LCYIL IIMHLDNFIVE+ PLA +LNLKPSK+
Sbjct: 301 RFTVIKPGQFGRSKDRSYFIDPQNEDKILCYILAIIMHLDNFIVEITPLAHELNLKPSKV 360

Query: 358 VSLFKILGATVKGATVAQAEAFGIPKSSASTYKIATLKVPFKLPEMIRRGKGPRR 412
           VSLF++LGA VKGATVAQAEAFGIPKS+A++YKIAT+KVPFKLPEM RRG+GPRR
Sbjct: 361 VSLFRVLGAIVKGATVAQAEAFGIPKSTAASYKIATMKVPFKLPEMTRRGRGPRR 415


>SPAC2F3.03c [K] KOG4183 RNA polymerase I 49 kDa subunit
          Length = 425

 Score =  159 bits (402), Expect = 7e-39
 Identities = 105/388 (27%), Positives = 188/388 (48%), Gaps = 16/388 (4%)

Query: 29  GFQAPKDTTFELYKNKKNSDKYV-LHGENDRLEYEGQTDDDFVNSSQYVVGVYDAATKSV 87
           GF  P++T F+L K    S++   L G  +R+++E +        + Y + V D   +  
Sbjct: 43  GFYPPENTRFQLLKKSNQSNRDASLIGRTERVQFEAKNQSTATVEANYCLAVADKTGRVK 102

Query: 88  ELYKAPXXXXXXXXXXRKNLMGPDIKQAN--VRAALM--RNALGEAFGTKKAKKAIADME 143
           ++  A                   +K+ +  V   +M  R  LG AFGT+K++KAI +  
Sbjct: 103 KIVPAKYLNTFERNILALQEKDKFLKKKHGTVSGTVMEQRANLGLAFGTRKSQKAIMEES 162

Query: 144 RNRIDSDKLVDSAIDIVDTVKTASKELPSKEELQETVSSDRPTPLADLDATDVEQIYPLY 203
            NR+ ++ L D    +V  V+ A++ LP++E++    + DRP P  ++ A  +E  Y L 
Sbjct: 163 ANRVKAETLGDVKDQLVSNVQKATEALPTQEDIAAAQAQDRPIPPVNVGAESIEDAYKLE 222

Query: 204 NIIPKKEWSFIRVGSIMKEQDPEKRLEYFPYTKFQYV---ANKLPTXXXXXXXXXXXXXX 260
           +IIPK+E+S I +  +++  D     +  PY    ++     +L +              
Sbjct: 223 DIIPKEEFSAIYIKPLLENPDERNWAKLLPYRHSLFINERFQRLLSIEEVDQKRARILYY 282

Query: 261 XXXXXXXXXDNRRVNNKTKLLERINNPPDALLNGILERFTISRPGQFGRSKDKSFFIDPQ 320
                      R V N+  L +++ +PP+ L++G+++RFT +       +   S  +  +
Sbjct: 283 ISLLQAFLFSRRSVGNRETLRKKLADPPEILIDGLIKRFTQT-------TGIGSVQVSSR 335

Query: 321 REDKLLCYILVIIMHLDNFIVEVPPLAQQLNLKPSKLVSLFKILGATVKGATVAQAEAFG 380
             DK++CYILV+ + +DN+  +V  LA  LN+K  K   LF+ +G  +   T  Q  A G
Sbjct: 336 EVDKIICYILVLCLIVDNYSTDVLTLANDLNVKTMKANELFRTVGCRIMAYTETQRMALG 395

Query: 381 IPKSSASTYKIATLKVPFKLPEMIRRGK 408
           + K+ A  +K A LK+P + P+  RRG+
Sbjct: 396 LNKTDAKNHKRAVLKIPLEFPKP-RRGR 422


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.316    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,366,018
Number of Sequences: 60738
Number of extensions: 899779
Number of successful extensions: 2416
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2407
Number of HSP's gapped (non-prelim): 2
length of query: 412
length of database: 30,389,216
effective HSP length: 109
effective length of query: 303
effective length of database: 23,768,774
effective search space: 7201938522
effective search space used: 7201938522
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)