ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0866 good A KOG0007 RNA processing and modification Splicing factor 3a, subunit 1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0866 299405 300559 385
(385 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YJL203w [A] KOG0007 Splicing factor 3a subunit 1 98 2e-20
SPAC22A12.09c [A] KOG0007 Splicing factor 3a subunit 1 98 2e-20
At1g14650 [A] KOG0007 Splicing factor 3a subunit 1 95 2e-19
At1g14640 [A] KOG0007 Splicing factor 3a subunit 1 93 6e-19
Hs5032087 [A] KOG0007 Splicing factor 3a subunit 1 80 7e-15
CE17296 [A] KOG0007 Splicing factor 3a subunit 1 73 6e-13
7300207 [A] KOG0007 Splicing factor 3a subunit 1 71 3e-12
>YJL203w [A] KOG0007 Splicing factor 3a subunit 1
Length = 280
Score = 98.2 bits (243), Expect = 2e-20
Identities = 74/285 (25%), Positives = 134/285 (46%), Gaps = 23/285 (8%)
Query: 33 PADESQRRSIWKTVEYVIRNGATYEEKL-KSQDIGFTQPGNKYNDYYAFLLEHQRSRQDT 91
P D + I TV Y+ ++G +E KL + + F + + ++YY L+ DT
Sbjct: 3 PEDTQLKEDIKTTVNYIKQHGVEFENKLLEDERFSFIKKDDPLHEYYTKLMNEPT---DT 59
Query: 92 LVSTKNEL--DRKPSEPYPFVYSTYRNDIGQKDLELIKAAAHFCVINNDINYLEKLRERY 149
+ N+ +R+ + P F++S Y I ++D+E+IK A + D + +E++ +
Sbjct: 60 VSGEDNDRKSEREIARPPDFLFSQYDTGISRRDMEVIKLTARYYA--KDKSIVEQMISKD 117
Query: 150 AEDPRFAFLRLDHSLNSVLTDFINQYEQILSEEYGPIVRLEGPLQSTILRRAFQKAEYME 209
E R F+ H L+ TDF+ QY+++ S I + + TIL F++ +Y E
Sbjct: 118 GE-ARLNFMNSSHPLHKTFTDFVAQYKRVYSFTGQEIKKSK----RTILDNCFERTQYWE 172
Query: 210 LQDELKQKMKADIKTSRIRFSAYEWEKFELVGTVTISEEE-------NLEQL---PEALN 259
+ + ++ ++ +I+F+A W+KF V +I E+ +LEQ+
Sbjct: 173 FEKDKDREHDKLVELCKIQFAAIPWDKFTQVAKFSIPEDTEIFEGSLDLEQMRLRRVQTG 232
Query: 260 FDILKATKLTNDVDIFKTEDVSAAAQKRKRKNMNIRKAGETRLKK 304
+ + K TN+ + ++ K K IR GETRLKK
Sbjct: 233 IKLFDSIKPTNEEEKIVSDQGKQKGGDSKGKKRKIRAVGETRLKK 277
>SPAC22A12.09c [A] KOG0007 Splicing factor 3a subunit 1
Length = 481
Score = 98.2 bits (243), Expect = 2e-20
Identities = 92/421 (21%), Positives = 168/421 (39%), Gaps = 78/421 (18%)
Query: 25 SVPDGVEVPADESQRRSIWKTVEYVIRNGATYEEKLKSQD-----IGFTQPGNKYNDYYA 79
SVP GV +P + R I K+ YV RNG +EEK++ + F + Y+ YY
Sbjct: 29 SVPAGVILPPP-AIREIIDKSASYVARNGPAFEEKIRQNEQANTKFAFLHANDPYHPYYQ 87
Query: 80 FLLEHQR-----------SRQDTLVSTKNELDRKPSE-----PYPFVYSTYRNDIGQKDL 123
L R S Q T ST +KP E P P+++S I DL
Sbjct: 88 HKLTEAREGKLKSHATGLSTQKT--STLARPIQKPIEATIPAPSPYLFSEPLPSISSLDL 145
Query: 124 ELIKAAAHFCVINNDINYLEKLRERYAEDPRFAFLRLDHSLNSVLTDFINQYEQILSEEY 183
++++ A + + ++L L ++ + +F FL+ +++L + QY ++ E
Sbjct: 146 DVLRLTARYAAVRGS-SFLVSLSQKEWNNTQFDFLKPNNALYPYFMRIVQQYTSLIRE-- 202
Query: 184 GPIVRLEGPLQSTI------LRRAFQKAEYMELQDELKQKMKADIKTSRIRFSAYEWEKF 237
PI E L+ + L + + + + K+K K + + ++ ++ +W F
Sbjct: 203 -PISSPEQELRENVRDPYSLLSKIQPRVRWQSHMESQKKKQKEEAEKEKLEYAQIDWNDF 261
Query: 238 ELVGTVTISEEENLEQLPEALNFDILKATKLTNDVDIFKTEDVSAAAQKR---------- 287
+V + ++ + +L + N L+ L +F D + ++
Sbjct: 262 VVVEVIQFTKSDEHAKLAKPTNLADLQTATLEQKSAMFTMPDQNYTIEEAPPTAEPWEPI 321
Query: 288 ---KRKNMNIRKAGETRLKKKSGLSNTSE------------------------------- 313
K++ + +K G+S+T+
Sbjct: 322 SAPKKQEFGVSLPPSLASPEKGGISSTTSVSPAAQASPVLSTTTQPKVQKPVPKAFQPKV 381
Query: 314 HMLKCPITGRLVPEPDFDSHLKILLSDPEFKEVRDKYESKHKLTNLSTSEVYENIKRIVS 373
M P +G LVP + + H+++ L DP ++E R ES+ NL V N+KR+VS
Sbjct: 382 PMEISPFSGELVPATELEEHMRLKLLDPRWQEQRKVEESRKSTLNLENVNVAANMKRLVS 441
Query: 374 Q 374
Q
Sbjct: 442 Q 442
>At1g14650 [A] KOG0007 Splicing factor 3a subunit 1
Length = 785
Score = 94.7 bits (234), Expect = 2e-19
Identities = 90/399 (22%), Positives = 176/399 (43%), Gaps = 71/399 (17%)
Query: 39 RRSIWKTVEYVIRNGATYEEKL-----KSQDIGFTQPGNKYNDYYAFLLEHQRSRQ---- 89
R + KT ++V +NG +E+++ K+ F + + Y+ +Y L R++
Sbjct: 69 RTIVEKTAQFVSKNGLEFEKRIIVSNEKNAKFNFLKSSDPYHAFYQHKLTEYRAQNKDGA 128
Query: 90 ----DTLVSTKNELD--------------------RKPSEPY----PFVYSTYRND-IGQ 120
D+ +T +LD R PS+P P Y+ + I
Sbjct: 129 QGTDDSDGTTDPQLDTGAADESEAGDTQPDLQAQFRIPSKPLEAPEPEKYTVRLPEGITG 188
Query: 121 KDLELIKAAAHFCVINNDINYLEKLRERYAEDPRFAFLRLDHSLNSVLTDFINQYEQILS 180
++L++IK A F V N ++L L R +P+F F++ HS+ + T ++ Y ++L
Sbjct: 189 EELDIIKLTAQF-VARNGKSFLTGLSNRENNNPQFHFMKPTHSMFTFFTSLVDAYSEVLM 247
Query: 181 --EEYGPIVRLEGPLQSTILRRAFQKAEYMELQDELKQKMKADIKTSRIRFSAYEWEKFE 238
++ +R +T+L R + E+ Q++ K+K + + + R++ + +W F
Sbjct: 248 PPKDLKEKLRKSAADLTTVLERCLHRLEWDRSQEQQKKKEEDEKELERVQMAMIDWHDFV 307
Query: 239 LVGTVTISEEENLEQLPEALNFDIL----KATKLTNDVDI---------FKTEDVSAAAQ 285
+V ++ ++EE+ E+LP + D + KA+ + D + E+V A+
Sbjct: 308 VVESIDFADEED-EELPPPMTLDEVIRRSKASAMEEDEIVEPGKEVEMEMDEEEVKLVAE 366
Query: 286 KRKRKNMNIRKAGET---------------RLKKKSGLSNTSEHMLKCPITGRLVPEPDF 330
+ N+ E R + + ++ PITG L+P +
Sbjct: 367 GMRAANLEENVKIENVHDEEAPMRIVKNWKRPEDRIPTERDPTKVVISPITGELIPINEM 426
Query: 331 DSHLKILLSDPEFKEVRDKYESKHKLTNLS-TSEVYENI 368
H++I L DP+FKE +D+ +K + T L+ E+ +NI
Sbjct: 427 SEHMRISLIDPKFKEQKDRMFAKIRETTLAQDDEIAKNI 465
>At1g14640 [A] KOG0007 Splicing factor 3a subunit 1
Length = 735
Score = 93.2 bits (230), Expect = 6e-19
Identities = 88/403 (21%), Positives = 182/403 (44%), Gaps = 65/403 (16%)
Query: 27 PDGVEVPADESQRRSIWKTVEYVIRNGATYEEKLKSQ-----DIGFTQPGNKYNDYYAF- 80
P G+ P E R+ + T ++V +NG + K+K++ + F + N Y+ +Y +
Sbjct: 57 PIGIIYPPPEI-RKIVETTAQFVSQNGLAFGNKVKTEKANNANFSFLKSDNPYHGFYRYK 115
Query: 81 LLEHQRSRQDTLVSTK----------NELDRKPS-------------EPYPFVYSTYRND 117
+ E+ +D T +E D KP P P Y+ +
Sbjct: 116 VTEYSCHIRDGAQGTDVDDTEDPKLDDESDAKPDLQAQFRAPRKILEAPEPEKYTVRLPE 175
Query: 118 -IGQKDLELIKAAAHFCVINNDINYLEKLRERYAEDPRFAFLRLDHSLNSVLTDFINQYE 176
I + +L++IK A F V N ++L +L R + +F F++ HS+ + T ++ Y
Sbjct: 176 GIMEAELDIIKHTAQF-VARNGQSFLRELMRREVNNSQFQFMKPTHSMFTFFTSLVDAYS 234
Query: 177 QILSEEYGPIVRLEGPLQ--STILRRAFQKAEYMELQDELKQKMKADIKTSRIRFSAYEW 234
++L +L + +T+L R + E+ Q+E K K + + + R++ +W
Sbjct: 235 EVLMPPRDLKEKLRKSVADLTTVLERCLNRLEWDRFQEEEKNKEEDEKEKERVQMVMIDW 294
Query: 235 EKFELVGTVTISEEENLEQLPEALNFD-ILKATKLT-----------NDVDI-FKTEDVS 281
+ F +V ++ ++EE+ + LP + + +++ +K++ +V++ E+V
Sbjct: 295 KDFAVVESIDFADEED-KDLPMPMTLEEVIRRSKVSAMEEDEIVEPGKEVEMDMDEEEVK 353
Query: 282 AAAQKRKRKNMNIRKAGETRLKKKSGL---------------SNTSEHMLKCPITGRLVP 326
A+ + N+ G +++++ + S ++ ITG L+P
Sbjct: 354 LVAEGMRAANLE-EYVGSVEIEEEAPMRIVKNWKRPEDRFLTERDSSKVVISRITGELIP 412
Query: 327 EPDFDSHLKILLSDPEFKEVRDKYESKHKLTNLS-TSEVYENI 368
+ H++I L DP+FKE +D+ +K + T L+ E+ +NI
Sbjct: 413 ITEMSEHMRISLIDPKFKEQKDRMFAKIRETTLAQDDEIAKNI 455
>Hs5032087 [A] KOG0007 Splicing factor 3a subunit 1
Length = 793
Score = 79.7 bits (195), Expect = 7e-15
Identities = 92/431 (21%), Positives = 165/431 (37%), Gaps = 88/431 (20%)
Query: 4 TDKGSKTSQATSLNMDSFDYLSVPDGVEVPADESQRRSIWKTVEYVIRNGATYEEKLKSQ 63
T+ T + S DS V G+ P E R + KT +V RNG +E +++
Sbjct: 17 TEPKQPTEEEASSKEDSAPSKPVV-GIIYPPPEV-RNIVDKTASFVARNGPEFEARIRQN 74
Query: 64 DIG-----FTQPGNKYNDYYAFLLEHQRS-------------RQDTLVSTKNELDRK--- 102
+I F P + Y+ YY + + Q +T+ +L +K
Sbjct: 75 EINNPKFNFLNPNDPYHAYYRHKVSEFKEGKAQEPSAAIPKVMQQQQQTTQQQLPQKVQA 134
Query: 103 --------PSEPYP-FVYSTYRNDIGQKDLELIKAAAHFCVINNDINYLEKLRERYAEDP 153
P EP P F + I DL+++K A F V N +L +L ++ +
Sbjct: 135 QVIQETIVPKEPPPEFEFIADPPSISAFDLDVVKLTAQF-VARNGRQFLTQLMQKEQRNY 193
Query: 154 RFAFLRLDHSLNSVLTDFINQYEQILSEEYGPIVRLEGPLQS--TILRRAFQKAEYMELQ 211
+F FLR HSL + T + QY +IL G +L+ ++ +L + + E+ + Q
Sbjct: 194 QFDFLRPQHSLFNYFTKLVEQYTKILIPPKGLFSKLKKEAENPREVLDQVCYRVEWAKFQ 253
Query: 212 DELKQKMKADIKTSRIRFSAYEWEKFELVGTVTISEEEN----LEQLPEALNFDIL---- 263
+ ++K + + + R+ ++ +W F +V TV E PE L IL
Sbjct: 254 ERERKKEEEEKEKERVAYAQIDWHDFVVVETVDFQPNEQGNFPPPTTPEELGARILIQER 313
Query: 264 -----KATKLTNDVDIFKTEDVSAAAQKRKRK---------------------------- 290
++ ++ +V+ + +D A++ +
Sbjct: 314 YEKFGESEEVEMEVESDEEDDKQEKAEEPPSQLDQDTQVQDMDEGSDDEEEGQKVPPPPE 373
Query: 291 ------------NMNIRKAGETRLKKKSGLSNTSEHMLKCPITGRLVPEPDFDSHLKILL 338
+ +RK + + K + + L PITG +P H++I L
Sbjct: 374 TPMPPPLPPTPDQVIVRKDYDPKASKPLPPAPAPDEYLVSPITGEKIPASKMQEHMRIGL 433
Query: 339 SDPEFKEVRDK 349
DP + E RD+
Sbjct: 434 LDPRWLEQRDR 444
>CE17296 [A] KOG0007 Splicing factor 3a subunit 1
Length = 655
Score = 73.2 bits (178), Expect = 6e-13
Identities = 78/356 (21%), Positives = 149/356 (40%), Gaps = 51/356 (14%)
Query: 39 RRSIWKTVEYVIRNGATYEEKLKSQD--------IGFTQPGNKYNDYYAFLLEHQRSRQD 90
R + KT + +NG +E K++ ++ + T P + Y + R
Sbjct: 35 RTIVDKTARFAAKNGVDFENKIREKEAKNPKFNFLSITDPYHAYYKKMVYDFSEGRVEAP 94
Query: 91 TLVSTKNELDRK----PSEPYP-FVYSTYRNDIGQKDLELIKAAAHFCVINNDINYLEKL 145
+ E +K PS P P + +S + I DL+LI+ A F V N +L +L
Sbjct: 95 KVPQAVKEHVKKAEFVPSAPPPAYEFSADPSTINAYDLDLIRLVALF-VARNGRQFLTQL 153
Query: 146 RERYAEDPRFAFLRLDHSLNSVLTDFINQYEQILSEEYGPIVRLEGPLQSTILRRAFQKA 205
R A + +F FL+ H + T ++QY+++L + +L+ +T +R +
Sbjct: 154 MTREARNYQFDFLKPAHCNFTYFTKLVDQYQKVLVPSTNVVAQLQD--DATNKKRLIEDI 211
Query: 206 EYM----ELQDELKQKMKADIKTSRIRFSAYEWEKFELVGTVTISEEENLEQLP------ 255
Y + Q LK + +A+ + R +++ +W F +V TV + + QLP
Sbjct: 212 NYRVSWEKHQKGLKDREEAEAEKERQAYASIDWHDFVVVQTVDF-QPGDTSQLPPLCTPK 270
Query: 256 --------EALNFDILKATKLTNDVDIFKTED-------------VSAAAQKRKRKNMNI 294
EA N ++ KA ++D+ +++ +A K+K++ +
Sbjct: 271 DVGARILLEARN-EMQKAAAEMQEMDMEESDSDDEDAVQAPEAPAFTAPLPPTKQKDVIV 329
Query: 295 RKAGETRLKKKSGLSNTSEHMLKCPITGRLVPEPDFDSHLKILLSDPEFKEVRDKY 350
R R + E+ + P+TG +P H++ D ++KE RD++
Sbjct: 330 RDYDPKR--NVTQKPKAVENWIISPLTGERIPSDKLAEHVRYNTVDSQYKEDRDRH 383
>7300207 [A] KOG0007 Splicing factor 3a subunit 1
Length = 784
Score = 70.9 bits (172), Expect = 3e-12
Identities = 62/250 (24%), Positives = 104/250 (40%), Gaps = 37/250 (14%)
Query: 29 GVEVPADESQRRSIWKTVEYVIRNGATYEEKLKSQDIG-----FTQPGNKYNDYYAFLLE 83
G+ P E R + KT +V RNG +E +++ ++G F G+ Y+ YY +
Sbjct: 27 GIIYPPPEV-RNIVDKTASFVARNGPEFEARIRQNELGNPKFNFLNGGDPYHAYYRHKVN 85
Query: 84 HQRS-----------------------RQDTLVSTKNELDRKPSEPYP-FVYSTYRNDIG 119
R RQ L+ E P EP P F + I
Sbjct: 86 EFREGNDAGITALASMKQLAVTSAAQQRQQELLKQVVEQQFVPKEPPPEFEFIADPPSIS 145
Query: 120 QKDLELIKAAAHFCVINNDINYLEKLRERYAEDPRFAFLRLDHSLNSVLTDFINQYEQIL 179
DL+++K A F V N +L L R + +F FLR HSL T + QY ++L
Sbjct: 146 ALDLDIVKLTAQF-VARNGRQFLTNLMSREQRNFQFDFLRPQHSLFQYFTKLLEQYTKVL 204
Query: 180 ---SEEYGPIVRLEGPLQST---ILRRAFQKAEYMELQDELKQKMKADIKTSRIRFSAYE 233
+ G + P +S+ +L + +A + Q+ +++ + I+ R+ ++ +
Sbjct: 205 IPPKDLLGKLRSESAPGRSSMNQVLEQVKYRANWQRHQEAQRRREEEKIERERVAYAQID 264
Query: 234 WEKFELVGTV 243
W F +V TV
Sbjct: 265 WHDFVVVETV 274
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.132 0.363
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 23,230,873
Number of Sequences: 60738
Number of extensions: 1027141
Number of successful extensions: 2634
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 2621
Number of HSP's gapped (non-prelim): 12
length of query: 385
length of database: 30,389,216
effective HSP length: 108
effective length of query: 277
effective length of database: 23,829,512
effective search space: 6600774824
effective search space used: 6600774824
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)