ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0954 good U KOG1655 Intracellular trafficking, secretion, and vesicular transport Protein involved in vacuolar protein sorting
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0954 330900 331574 225
(225 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR486c [U] KOG1655 Protein involved in vacuolar protein sorting 239 2e-63
SPCC162.06c [U] KOG1655 Protein involved in vacuolar protein sor... 119 3e-27
7293963 [U] KOG1655 Protein involved in vacuolar protein sorting 100 3e-21
CE10260 [U] KOG1655 Protein involved in vacuolar protein sorting 96 3e-20
Hs20127558 [U] KOG1655 Protein involved in vacuolar protein sorting 92 8e-19
At5g04850 [U] KOG1655 Protein involved in vacuolar protein sorting 91 2e-18
At3g10640 [U] KOG1655 Protein involved in vacuolar protein sorting 87 1e-17
YLR025w [U] KOG1656 Protein involved in glucose derepression and... 57 3e-08
7291437 [R] KOG2910 Uncharacterized conserved protein predicted ... 54 2e-07
CE02850 [U] KOG1656 Protein involved in glucose derepression and... 52 7e-07
>YDR486c [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 262
Score = 239 bits (610), Expect = 2e-63
Identities = 121/216 (56%), Positives = 154/216 (71%), Gaps = 4/216 (1%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
MNRIFGYGN+K+H+Q++Q+S+++M +AQ LS RISQLDTQI+QLNFQL +QK + +
Sbjct: 34 MNRIFGYGNKKSHDQLLQESNQSMNQAQQSLSNRISQLDTQIAQLNFQLQNIQKNLQ--R 91
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
S+ Q LR++ALK+LNKRKQLE M+D LDSQSWSM+Q Q+T DNL+NTM+T+NALKQTN
Sbjct: 92 SNNKQPSLRKQALKILNKRKQLENMKDSLDSQSWSMTQAQLTNDNLQNTMITINALKQTN 151
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVLAMQNQXXXXXXXXXXXXXXXXXXX 180
A+K QY KINIDK+QDMQDEM+DLIEQGDELQ+VLAM N
Sbjct: 152 NAMKAQYGKINIDKLQDMQDEMLDLIEQGDELQEVLAMNNNSGELDDISDAELDAELDAL 211
Query: 181 XXXXXXXXXXXSETTGELPSYL--NNAMPQFVDDEP 214
+ ++PSYL NA P F+D+EP
Sbjct: 212 AQEDFTLPTSENSLGNDMPSYLLGANAPPAFIDEEP 247
>SPCC162.06c [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 210
Score = 119 bits (298), Expect = 3e-27
Identities = 75/218 (34%), Positives = 118/218 (53%), Gaps = 15/218 (6%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
M+R+FG RK Q + ++ +A L R ++ +I++L+ QLS Q+KI+NT+
Sbjct: 1 MHRLFG---RKPPTQ----PTASLTDAIDSLDKRSDSVEVKIAKLDAQLSVFQQKIANTR 53
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
GQ L+QRA+ +L ++K E+ QL QS++M Q MTT++LKNTM TV +++T
Sbjct: 54 PGPGQTALKQRAMNVLRQKKIYESQLQQLQQQSFNMEQAAMTTESLKNTMATVQTMQETA 113
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVLAMQNQXXXXXXXXXXXXXXXXXXX 180
+ LK Q ++I+KI+ +QDE+ D ++ EL +VL QN
Sbjct: 114 RQLKSQSKNVSIEKIEKLQDEIQDYMDAAGELNEVLG-QNM-----TDINVDEEELDAEL 167
Query: 181 XXXXXXXXXXXSETTGELPSYL--NNAMPQFVDDEPLE 216
++T E PSYL +N +P FVD+E E
Sbjct: 168 EALQQESSWLGDQSTAEKPSYLMPSNELPNFVDEEVAE 205
>7293963 [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 145
Score = 99.8 bits (247), Expect = 3e-21
Identities = 49/152 (32%), Positives = 90/152 (58%), Gaps = 7/152 (4%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
MNR+FG G K + ++ + +G+ R + ++ +IS L +L ++++S +
Sbjct: 1 MNRLFGRGKPK-------EPGPSLNDCIAGVDARATNIEEKISNLEAELRKYREQMSKMR 53
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
+ ++Q+AL++L ++K E + L +QS++M Q +LK+T TV A+K
Sbjct: 54 EGPAKNSVKQKALRVLKQKKAYEQQAESLRNQSFNMEQANYAAQSLKDTQATVAAMKDGV 113
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDEL 152
+ +K +Y KINID+I+D+QD+M D+ EQ DE+
Sbjct: 114 KQMKTEYKKINIDQIEDIQDDMADMFEQADEV 145
>CE10260 [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 217
Score = 96.3 bits (238), Expect = 3e-20
Identities = 47/159 (29%), Positives = 92/159 (57%), Gaps = 8/159 (5%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
MNRIFG G + + A S + R +D +I +L+ L L+ ++S +
Sbjct: 1 MNRIFGTGKKVPPPDL--------NNAISNVESRSDSIDKKIQKLDQDLMKLRDQMSKMR 52
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
+ ++Q+AL++L +++ E + QLD Q+++M Q +K+ VTV A+K
Sbjct: 53 EGPSKNLIKQKALRVLKQKRMYENQKGQLDQQAFNMDQSNFAIQGMKDNQVTVAAMKDGL 112
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVLAMQ 159
+ ++++Y K+NID+I+D+QD+M D+++ +E+Q+ ++ Q
Sbjct: 113 KTMQKEYKKMNIDQIEDLQDQMEDMLDMNNEIQEAMSRQ 151
>Hs20127558 [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 219
Score = 91.7 bits (226), Expect = 8e-19
Identities = 48/157 (30%), Positives = 91/157 (57%), Gaps = 7/157 (4%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
MNR+FG K + + +G S R +D +IS+L+ +L + +I +
Sbjct: 1 MNRLFGKAKPKAPPPSL---TGCIGTVDS----RAESIDKKISRLDAELVKYKDQIKKMR 53
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
+ ++Q+AL++L +++ E RD L QS++M Q T +LK+T TV+A+K
Sbjct: 54 EGPAKNMVKQKALRVLKQKRMYEQQRDNLAQQSFNMEQANYTIQSLKDTKTTVDAMKLGV 113
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVLA 157
+ +K+ Y ++ ID+I+D+QD++ D++E +E+Q+ L+
Sbjct: 114 KEMKKAYKQVKIDQIEDLQDQLEDMMEDANEIQEALS 150
>At5g04850 [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 235
Score = 90.5 bits (223), Expect = 2e-18
Identities = 46/156 (29%), Positives = 84/156 (53%), Gaps = 7/156 (4%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
M RIFG N K +QD+S + + + ++ +LD ++ + Q I T+
Sbjct: 1 MKRIFGAKNNKEPPPSIQDASDRINKRGDSVEEKVKRLDAELCKYKDQ-------IKRTR 53
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
+ ++ RA+++L ++K E RD L +Q++++ QV + LK+ T+ ALK N
Sbjct: 54 PGPALEAIKARAMRVLKQKKMYEGQRDMLYNQTFNLDQVSFAAEGLKDAQQTMTALKSAN 113
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVL 156
+ LK + I I ++QD+MMDL+++ E+Q+ L
Sbjct: 114 KELKGMMKTVKIQDIDNLQDDMMDLMDESSEIQETL 149
>At3g10640 [U] KOG1655 Protein involved in vacuolar protein sorting
Length = 221
Score = 87.4 bits (215), Expect = 1e-17
Identities = 46/156 (29%), Positives = 82/156 (52%), Gaps = 7/156 (4%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
M R+FG +QD+S + + + +I +LD ++ + QL T+
Sbjct: 1 MRRVFGAKKNTEPPPSIQDASDRINKRGDSVEDKIKKLDVELCKYKEQLK-------KTR 53
Query: 61 SSMGQKPLRQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTN 120
Q+ ++ RA+++L ++K E RD L +Q++++ QV + LK+ T+ ALK N
Sbjct: 54 PGPAQEAVKARAMRVLKQKKMYEGQRDMLYNQTFNLDQVSFAAEGLKDAQQTMTALKSAN 113
Query: 121 QALKQQYSKINIDKIQDMQDEMMDLIEQGDELQDVL 156
+ LK + I I ++QDEMMDL++ E+Q+ L
Sbjct: 114 KELKGMMKTVKIQDIDNLQDEMMDLMDVSSEIQESL 149
>YLR025w [U] KOG1656 Protein involved in glucose derepression and
pre-vacuolar endosome protein sorting
Length = 240
Score = 56.6 bits (135), Expect = 3e-08
Identities = 34/129 (26%), Positives = 73/129 (56%), Gaps = 9/129 (6%)
Query: 35 ISQLDTQISQLNFQLSTLQKKISNTKS------SMGQKPLRQRALKLLNKRKQLEAMRDQ 88
I +L I+ L+ + S L+ +I+N ++ + G K + + ALK K+K +E + +
Sbjct: 23 IVRLREHINLLSKKQSHLRTQITNQENEARIFLTKGNKVMAKNALK---KKKTIEQLLSK 79
Query: 89 LDSQSWSMSQVQMTTDNLKNTMVTVNALKQTNQALKQQYSKINIDKIQDMQDEMMDLIEQ 148
++ SM Q + ++ + T+ A+++ +A+K +S ++IDK+ + DE+ + +E
Sbjct: 80 VEGTMESMEQQLFSIESANLNLETMRAMQEGAKAMKTIHSGLDIDKVDETMDEIREQVEL 139
Query: 149 GDELQDVLA 157
GDE+ D ++
Sbjct: 140 GDEISDAIS 148
>7291437 [R] KOG2910 Uncharacterized conserved protein predicted to be
involved in protein sorting
Length = 212
Score = 53.5 bits (127), Expect = 2e-07
Identities = 44/169 (26%), Positives = 81/169 (47%), Gaps = 20/169 (11%)
Query: 1 MNRIFGYGNRKTHEQMMQDSSKAMGEAQSGLSGRISQLDTQISQLNFQLSTLQKKISNTK 60
M +FG ++KT + D KA + QL Q +L ++ ++ N +
Sbjct: 1 MGALFGKTSKKTAPSRITDQDKA-----------VLQLKQQRDRLKQYQKRIETQLENDR 49
Query: 61 SSMGQKPLRQ----RALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNAL 116
+ +K L+Q RA LL K+K E++ D Q ++ ++ + + M ++ L
Sbjct: 50 L-LARKCLQQGRKDRAKLLLRKKKYQESLLTNADKQLENLEKLAADIEFAQVEMKVLDGL 108
Query: 117 KQTNQALKQQYSKINIDKIQDMQDEMMDLIEQGDE----LQDVLAMQNQ 161
K N ALK+ + ++ID+++ + DE + IE+ E L DVL Q++
Sbjct: 109 KAGNAALKKVHEMLDIDEVERIMDETREGIEKQQEIDAILTDVLTTQDE 157
>CE02850 [U] KOG1656 Protein involved in glucose derepression and
pre-vacuolar endosome protein sorting
Length = 222
Score = 52.0 bits (123), Expect = 7e-07
Identities = 33/149 (22%), Positives = 73/149 (48%), Gaps = 5/149 (3%)
Query: 11 KTHEQMMQDSSKAMGEAQSGLSGRISQLDTQI--SQLNFQLSTLQKKISNTKSSMGQKPL 68
K E++ + S K G + + + Q F+L +++++ + K M K
Sbjct: 19 KIKEKIKEKSRKRKAPTADEAIGNLRDAEELLIKKQEYFELR-IEQEVESAKKYM--KTN 75
Query: 69 RQRALKLLNKRKQLEAMRDQLDSQSWSMSQVQMTTDNLKNTMVTVNALKQTNQALKQQYS 128
++ AL L K+K E ++D + + +N+ ++ L +T + LK++++
Sbjct: 76 KKMALAALRKKKHYEQELSRIDGVLTKLEAQRTALENVGMHNEVIDVLGKTTETLKKEHA 135
Query: 129 KINIDKIQDMQDEMMDLIEQGDELQDVLA 157
K++IDK+ D+ DE+ D + +EL + ++
Sbjct: 136 KMDIDKVHDLMDEIADGLAMSEELNEAIS 164
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.309 0.124 0.323
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,786,179
Number of Sequences: 60738
Number of extensions: 291054
Number of successful extensions: 1499
Number of sequences better than 1.0e-05: 10
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1483
Number of HSP's gapped (non-prelim): 10
length of query: 225
length of database: 30,389,216
effective HSP length: 102
effective length of query: 123
effective length of database: 24,193,940
effective search space: 2975854620
effective search space used: 2975854620
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)