ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII0970 good L KOG2108 Replication, recombination and repair 3'-5' DNA helicase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII0970 337289 335166 -708
(708 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL095c [L] KOG2108 3'-5' DNA helicase 542 e-154
SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase 108 2e-23
YJL092w [L] KOG2108 3'-5' DNA helicase 82 3e-15
>YOL095c [L] KOG2108 3'-5' DNA helicase
Length = 706
Score = 542 bits (1396), Expect = e-154
Identities = 311/725 (42%), Positives = 457/725 (62%), Gaps = 38/725 (5%)
Query: 2 MKRPTDSQNEIIQFPYIPASTLKVIAGPGSGKTYTLLCKVRELIQTGQVKPREIIILSLT 61
M + T SQ ++I Y PAST+KVIAGPGSGKT TLL KV LI +KP EI+I SLT
Sbjct: 1 MDKLTPSQWKVINKSYEPASTIKVIAGPGSGKTLTLLYKVLHLITVENIKPEEILIFSLT 60
Query: 62 NKAVDNITDNLLGVFQELSDKPYTDHEVDDIVSQIGIYTIHGLSNRIVTENMGIVNIIEE 121
NKAVD+I +NLL +F+ H +IV QIG YT+HGL+NRIV EN G++NIIEE
Sbjct: 61 NKAVDSIIENLLSIFEN-------SHTNKEIVHQIGCYTVHGLANRIVVENEGMINIIEE 113
Query: 122 NGWRGLVKLIPKDFWNKRNVNTANDRIITKKLQKLINEYQIDT-DNIDETIEKVTKLMKH 180
GWRGL+KL+P +KR T + K+L+K++ +Y+++ N + IEK+ +LM +
Sbjct: 114 IGWRGLMKLLPP---SKR---TPHHFRSYKELEKVVKDYKLNNAKNNNPVIEKLVELMDN 167
Query: 181 SNVLTNDDLIKMAVEFLKEPNSEED-TAFTKFVLNEAKVLIIDEFQDLYPGIFKLLKTLL 239
V+TNDDLI A ++L+ +S+ D ++FT+ + N+ KV++IDEFQDLYP + L+ +
Sbjct: 168 CKVMTNDDLIIRAKKYLELDSSDSDASSFTQDLRNKYKVVLIDEFQDLYPSLAPLITMIC 227
Query: 240 GQKHFLIFGDINQSIYEFLGSNKEVMSELDRLRPDDKKFTKYIHDNFRSTPEITAAANAV 299
K ++FGD NQSIY FLGSN E+MS+LD L P + + DNFRSTPEI + A+ +
Sbjct: 228 KGKQLIMFGDTNQSIYGFLGSNNEIMSQLDNLHPKNSTTVLKLFDNFRSTPEIISLASKI 287
Query: 300 INQTVPVQIKR---------ENIVEKHPSGVYPIKYNCEELIDELDFIVSEICQLVCSSA 350
IN+ P+ K+ +V K PSGV P ++L E +FI+ +I QL+CSSA
Sbjct: 288 INR--PLAEKQIIDDTDETPSELVRKLPSGVSPQIMTFDDLAAESEFIIDKITQLICSSA 345
Query: 351 KLSDIGILARTNSHSKAIADHLSTFGIPVSKLTTQPDWMSDQRIRFMIDLLQVAVSVIEE 410
K SDI IL+RTNSH AIA L +GIP KL +QPDWM D RI+F++D+L+V S+ +
Sbjct: 346 KFSDIAILSRTNSHLTAIASILKKYGIPYQKLKSQPDWMDDLRIQFLLDILKVC-SLASD 404
Query: 411 ATLPKDASFTMKTKSNFSVIVTLAALKGFGSNTLQKLHENATATGASIWHFLTNTPVSKW 470
++ + K +SNFS++VT++ALKG G ++Q L++ + SIW +LT P +W
Sbjct: 405 EKHNREFNTGDKWQSNFSILVTMSALKGIGDASIQALYKACSLKNLSIWKYLTMVPNFEW 464
Query: 471 GFTPAVTKKLENYLLAVSTLINDPPILEVEQPHILIDNCSSVISKLDL--PLFMCDSNSE 528
++ KK+ENY + +I + + +++ P L++ +S+ + L+L F S+++
Sbjct: 465 PLGLSIKKKMENYTSNLYEMIENDQVHQLDDPMELLEKVASITNNLNLNPTYFQSLSDAQ 524
Query: 529 SG-SFQVNLKEFYSAIKLSLLSKPQDLPTATWFLETFHDQSIIYHNNQKSITQEQNTLGS 587
S F+ +L+E +K+S +KP + WFLET+ DQ++++H +Q+++ + G+
Sbjct: 525 SSLEFKTHLQEMAQVMKVSKSNKPPGISFVKWFLETYFDQTMVFHQSQQAL--QTTGPGT 582
Query: 588 VNVSTIHCAKGLEFPIVFLMGRTDYGNFPIETNALYVAVTRARNLLYLMNVQH-RSLSAF 646
V +STIH AKGLEFPIVFL + NFP++TNALYV +TRARNLLY+ N++H R +S
Sbjct: 583 VKLSTIHSAKGLEFPIVFLTNGS-MSNFPMDTNALYVGITRARNLLYMCNMKHERLVSKS 641
Query: 647 PDRQSSLLQNQAFWKYYNSDLKRPFHASNVN---NAMKFKKLQTKFGLMNRYFHSLSQSL 703
+++ N FW YYN DLKR V N ++ +L+ FG R + SL
Sbjct: 642 SPYSRNIMSNNLFWTYYNKDLKRSVCDVKVTHGYNVQRYNQLRKNFGFY-RAYSSLRGCK 700
Query: 704 KILRR 708
+ RR
Sbjct: 701 SVFRR 705
>SPAC4H3.05 [L] KOG2108 3'-5' DNA helicase
Length = 887
Score = 108 bits (271), Expect = 2e-23
Identities = 158/684 (23%), Positives = 295/684 (43%), Gaps = 98/684 (14%)
Query: 19 PASTLKVIAGPGSGKTYTLLCKVRELIQTGQVKPREIIILSLTNKAVDNITDNLLGVFQE 78
P +++AGPGSGKT L +V L+Q + ++II + TNKA + I + +
Sbjct: 22 PHKYTQILAGPGSGKTRVLTARVAYLLQKNHIAAEDLIIATFTNKAANEIKLRIEAILG- 80
Query: 79 LSDKPYTDHEVDDIVSQIGIYTIHGLSNRIVTE---NMGIVN---IIEENGWRGLVKLIP 132
+ E ++S T H ++ + + + ++G+ + I + N + ++K +
Sbjct: 81 -------NSEASKLISG----TFHSIAYKYLVKYGKHIGLSSNWLIADRNDTQAIMKRLL 129
Query: 133 KDFWNKRNV------------NTANDRIITKKLQKLINEYQIDTDNIDETIEKVTK-LMK 179
+N A +RI K L+ + +D ++ +E+ K L
Sbjct: 130 DSLKKAKNPIASGIRGQELTPQNALNRITKLKSNGLLVKPGMDQLSLINGLEEPPKELQS 189
Query: 180 HSNVLTNDDLIKMAVEFLKEPN-SEEDTAFTKFVL---------NEAKVLIIDEFQDLYP 229
H +V +L ++ L + N ++ D F+L K ++IDEFQD
Sbjct: 190 HQSV----ELYRLYQTSLWKNNLADFDDLLLNFILLLQKQPDCVRNIKHILIDEFQDTSK 245
Query: 230 GIFKLLKTL-LGQKHFLIFGDINQSIYEFLGSNKEVMSELDRLRPDDKKFTKYIH--DNF 286
+ L+K L L I GD +QSIY F + ++++ +D + T+ +H N+
Sbjct: 246 IQYFLVKLLALQNSDITIVGDPDQSIYGFRSAEIRNLNQMS----EDFEGTQVLHLERNY 301
Query: 287 RSTPEITAAANAVINQTVPVQIKRENIVEKHPSGVYPIKYNCEELIDELDFIVSEICQLV 346
RS I A ++I+Q ++ + H S + P E E +I EI ++V
Sbjct: 302 RSAKPILELALSIISQDK--SRPKKGLKSNHISSLKPHYRLFETNNKESYWIAREIKRIV 359
Query: 347 CSSAKL---SDIGILARTNSHSKAIADHLSTFGIPVSKLTTQPDWMSDQRIRFMIDLLQV 403
S +L +DI IL R++S ++++ LS G+P ++ + + IR +I L+V
Sbjct: 360 GSCPELIFYNDIAILVRSSSLTRSLEHALSELGVPY-RMVGVNKFFDREEIRDLIAYLRV 418
Query: 404 AVSVIEEATLPKDASFTMKTKSNFSVIVTLAALKGFGSNTLQKLHENATATGASIWHFLT 463
+ KD++ ++ V + G + ++ + G + W L
Sbjct: 419 LAN--------KDSTSLIR--------VINVPPRNIGKTKIDRIIFESERRGLTFWQTLN 462
Query: 464 -----NTPVSKWGFTPAVTKKLENYLLAVSTLINDPPILEVEQPHILIDNCSSVISKLDL 518
N +S+ + K L+++L ++S L N L L D ++S++
Sbjct: 463 EVKNENILLSQRN-DKSFLKSLKSFLCSISKLENR--YLSNGHSATLSDLLLGILSEIKY 519
Query: 519 PLFMCDSNSES--GSFQVNLKEFYSAIKLSLLSKPQDLPTATW-FLETFHDQSIIYHNNQ 575
++ N E+ ++ ++ + +S + D +T L+ F Q + + Q
Sbjct: 520 YEYLVRKNKETVEEKWENVMELVQQSDNISCIFYELDYKISTIVLLQNFLTQIALVNEEQ 579
Query: 576 KSITQEQNTLGSVNVSTIHCAKGLEFPIVFL-------MGRTDYGNFPIETNALYVAVTR 628
K ++ V +ST+H AKGLE+P+VFL + + + E LYV TR
Sbjct: 580 KEGESQK-----VTISTLHAAKGLEWPVVFLPCLCENIIPHSRSDDLDEERRLLYVGATR 634
Query: 629 ARNLLYLMNVQHRSLSAFPDRQSS 652
A+ LLYL + + + F D Q+S
Sbjct: 635 AQALLYLSSFKSVT-GMFADMQNS 657
>YJL092w [L] KOG2108 3'-5' DNA helicase
Length = 1174
Score = 82.0 bits (201), Expect = 3e-15
Identities = 159/682 (23%), Positives = 261/682 (37%), Gaps = 144/682 (21%)
Query: 23 LKVIAGPGSGKTYTLLCKVRELIQTGQVKPREIIILSLTNKAVDNITDNLLGVFQELSDK 82
L+VIAGPG+GKT L +V LI + PR+II+ + TNKA + + + L + +
Sbjct: 31 LQVIAGPGTGKTKVLTSRVAYLILHHHIHPRDIIVTTFTNKAANEMKERLQEMLRGAGVN 90
Query: 83 PYTDHEVDDIVSQIGIYTIHGLSNRIVTENMGIVNIIEENGWR------------GLVKL 130
+S++ I T H + +I+ +V++ + WR +++
Sbjct: 91 ----------ISELLIGTFHSICLKILYRFGHLVDL--QKDWRIIDEKEIDVILDDMIEK 138
Query: 131 IP---KDFWNK--RNVN-----------TANDRIITKKLQKLIN------EYQIDTDNID 168
+P +D+ + R VN T + ++I K++ KL + EY +D+ N D
Sbjct: 139 VPDQIRDYASSITRKVNLCMPSKNGDEWTIHPKLIKKQISKLKSNAILPEEYILDS-NHD 197
Query: 169 ETIEKVTKL----MKHSNVLTNDDLIKMAVEFLKEPNSEEDTAFTKFVLNEAKVLIIDEF 224
+ ++ + N L DDL+ L VL+ K +++DEF
Sbjct: 198 AALGYFYQIYQSELSKKNTLDFDDLLMYTFRLLTRVR----------VLSNIKHVLVDEF 247
Query: 225 QDLYPGIFKLLKTLLGQKHFL-----IFGDINQSIYEFLGSNKEVMSELDRLRPDDKKFT 279
QD L+ H L I GD +QSIY F + E+ R P + T
Sbjct: 248 QDTNGIQLDLMFLFAKGNHHLSRGMTIVGDPDQSIYAFRNALAHNFLEMGRKCPIEYS-T 306
Query: 280 KYIHDNFRSTPEITAAANAVINQTVPVQIKRENIVEKHPSGVYPIKYNCEELIDELDFIV 339
+ +N+RS+ +I + +I Q + R + + P+ N E +V
Sbjct: 307 IILVENYRSSQKILNTSEILITQQNKGRQNRAPLRAQFDLDFPPVYMNFPAYFLEAPSLV 366
Query: 340 SEICQL--VCSSAKLSDIGILARTNSHSKAIADHLSTFGIPVSKLTTQPDWMSDQRIRFM 397
E+ L + + +D IL R K I L IP + W S + R M
Sbjct: 367 RELLYLKALPNLFTFNDFAILVRQRRQIKRIESALIEHRIPYKIIRGHSFWDS-KETRAM 425
Query: 398 IDLLQVAVSVIEEATLPKDASFTMKTKSNFSVIVTLAALKGFGSNTLQKLHE--NATATG 455
++LL++ S P D + + L +G G T +K+ + AT
Sbjct: 426 LNLLKLIFS-------PNDKHAILAS--------LLYPARGLGPATGEKIKNALDTLATD 470
Query: 456 ASIWHFL---------TNTPVSKWGFTPAVTKKLEN-YLLAVSTLI-------------- 491
S + L + P +EN LL STL+
Sbjct: 471 VSCFQILKDISSKKIMLDIPTKGRSVIADFISMIENCQLLLQSTLLGGLSDLFDKLYELS 530
Query: 492 --------------NDPPILEVEQPHILIDNCSSVISKLDLPLFMCDSNSESGSFQVNLK 537
ND LE +P++L + I L S SES K
Sbjct: 531 GLKYEYLYKDGKKKNDQ--LEKSEPNLLNARHKN-IELLKNYFLALLSKSESSD-----K 582
Query: 538 EFYSAIKLSL-LSKP--QDLPTATWFLETFHDQSIIYHNNQKSITQEQNTLGSV------ 588
E AIK + ++P + T +L F + ++ + + E N +
Sbjct: 583 EKNEAIKAATDEAEPIENKVITPKEYLRNFFNSLSLHSDAAEEEESESNKDAKIKREKNG 642
Query: 589 --NVSTIHCAKGLEFPIVFLMG 608
+STIH AKGLE+P+VF+ G
Sbjct: 643 FVTISTIHGAKGLEWPVVFIPG 664
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.135 0.386
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,543,575
Number of Sequences: 60738
Number of extensions: 1727501
Number of successful extensions: 5205
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 5189
Number of HSP's gapped (non-prelim): 6
length of query: 708
length of database: 30,389,216
effective HSP length: 114
effective length of query: 594
effective length of database: 23,465,084
effective search space: 13938259896
effective search space used: 13938259896
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)