ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1131 good I KOG1639 Lipid transport and metabolism Steroid reductase required for elongation of the very long chain fatty acids
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1131 400590 399634 -319
(319 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDL015c [I] KOG1639 Steroid reductase required for elongation of... 343 1e-94
SPBC646.07c [I] KOG1639 Steroid reductase required for elongatio... 140 3e-33
7292402 [I] KOG1639 Steroid reductase required for elongation of... 116 5e-26
CE20509 [I] KOG1639 Steroid reductase required for elongation of... 115 8e-26
At3g55360 [I] KOG1639 Steroid reductase required for elongation ... 88 1e-17
>YDL015c [I] KOG1639 Steroid reductase required for elongation of the very
long chain fatty acids
Length = 310
Score = 343 bits (881), Expect = 1e-94
Identities = 169/305 (55%), Positives = 213/305 (69%), Gaps = 7/305 (2%)
Query: 16 LKDVKLEVPTENTLTYQSVLQQISKSNHNISVNRLRLSYLKEGKQVAIGPSELNDVGKKN 75
L+D ++++ + TL VL++IS +NHNIS R+RL+Y KE KQV + +++ +
Sbjct: 12 LRDTEIDLSKKPTL--DDVLKKISANNHNISKYRIRLTYKKESKQVPV----ISESFFQE 65
Query: 76 TFDSVNEWYVKDLGPQISWRLVFFIEYLGPILIHSLVYLLSLNATVRDKFHSKNVPYNDF 135
D E+++KDLGPQISWRLVFF EYLGP+L+HSL Y LS TV D++HS + YN F
Sbjct: 66 EADDSMEFFIKDLGPQISWRLVFFCEYLGPVLVHSLFYYLSTIPTVVDRWHSASSDYNPF 125
Query: 136 FNKFIYRLIMVHYLKREFETLFIHSFSLETMPLFNLFKNSFHYWILNGLISLGYFGYGFP 195
N+ Y LI+ HY KR FETLF+H FSL TMP+FNLFKN FHYW+L+GLIS GYFGYGFP
Sbjct: 126 LNRVAYFLILGHYGKRLFETLFVHQFSLATMPIFNLFKNCFHYWVLSGLISFGYFGYGFP 185
Query: 196 FANKTLYRVYSALKISDFRVLTALFGLSEMFNFYIHVALRRWGDEQKRNGVTK-RVPLNS 254
F N L++ YS LK+ D L LF LSE++NFY H+ LR WGD QK++G K RVPLN
Sbjct: 186 FGNAKLFKYYSYLKLDDLSTLIGLFVLSELWNFYCHIKLRLWGDYQKKHGNAKIRVPLNQ 245
Query: 255 GLFKLLVAPNYTFESWAWMFFTXXXXXXXXXXXXXXXXXXQMYLWAQKKNKKYGTKRAFL 314
G+F L VAPNYTFE W+W++FT QMY WAQKKNKKY T+RAFL
Sbjct: 246 GIFNLFVAPNYTFEVWSWIWFTFVFKFNLFAVLFLTVSTAQMYAWAQKKNKKYHTRRAFL 305
Query: 315 IPFLF 319
IPF+F
Sbjct: 306 IPFVF 310
>SPBC646.07c [I] KOG1639 Steroid reductase required for elongation of the very
long chain fatty acids
Length = 295
Score = 140 bits (352), Expect = 3e-33
Identities = 91/241 (37%), Positives = 127/241 (51%), Gaps = 27/241 (11%)
Query: 84 YVKDLGPQISWRLVFFIEYLGPILIHSLVYLLSLNATVRDKFHSKNVPYNDFFNKFIYRL 143
+VKDLGPQI WR VF IEYLGP++IH L ++L+ R + N+ N K + L
Sbjct: 73 WVKDLGPQIGWRTVFMIEYLGPLVIH-LFFILNYKWIYRKDY---NLCLNQ---KIAFVL 125
Query: 144 IMVHYLKREFETLFIHSFSLETMPLFNLFKNSFHYWILNGLISLGYFGYGFPFANKTLYR 203
+M+H++KRE+E++F+H FSL TMPL N+FKN HY +L+GL L YF YG AN
Sbjct: 126 VMLHFMKREYESIFVHRFSLATMPLRNIFKNCAHYHLLSGLF-LAYFIYGPWHAN----- 179
Query: 204 VYSALKISDFRVLTALFGLSEMFNFYIHVALRRWGDEQKRNGVTKRVPLNSGLFKLLVAP 263
+K + L + + + NF H+ LR + + G KRV F L+ P
Sbjct: 180 --DYIKPNHLLFLIVGWAFAVLSNFRTHIILR----DLRPAGSKKRVIPTGYGFNLVSFP 233
Query: 264 NYTFESWAWMFFTXXXXXXXXXXXXXXXXXXQMYLWAQKKNKK-------YGTKRAFLIP 316
NY FES W+FF QM++WA+KK+ + Y R +IP
Sbjct: 234 NYFFESLGWLFF-ALLTKSWASWIFLFVGSAQMFVWAKKKHARYLKEFPNYPRSRKIMIP 292
Query: 317 F 317
F
Sbjct: 293 F 293
>7292402 [I] KOG1639 Steroid reductase required for elongation of the very
long chain fatty acids
Length = 302
Score = 116 bits (290), Expect = 5e-26
Identities = 83/245 (33%), Positives = 114/245 (45%), Gaps = 28/245 (11%)
Query: 84 YVKDLGPQISWRLVFFIEYLGPILIHSLVYLLSLNATVRDKFHSKNVPYNDFFNKFIYRL 143
YVKDLGPQI W+ VF EY GP++++ + Y V K S + Y
Sbjct: 75 YVKDLGPQIGWKTVFLAEYAGPLIVYLIFYFRP--ELVYGKAASLPISLTTHIAAGCYT- 131
Query: 144 IMVHYLKREFETLFIHSFSLETMPLFNLFKNSFHYWILNGLISLGYFGYGFPFANKTLYR 203
VHY+KR ET+F+H FS TMPL NLFKN +YW +S Y F + +
Sbjct: 132 --VHYVKRLLETIFVHRFSHATMPLRNLFKNCTYYWGFTAYVS--YHVNHPQFTSPCMCT 187
Query: 204 VYSALKISDFRVLTALFGLSEMFNFYIHVALRRW---GDEQKRNGVTKRVPLNSGLFKLL 260
V+ AL F L E+ NF +H+ALR G + ++ V PL LF L+
Sbjct: 188 VWGAL---------GAFALCELGNFSVHIALRNLRPPGTKVRKIPVADGNPLTK-LFDLV 237
Query: 261 VAPNYTFESWAWMFFTXXXXXXXXXXXXXXXXXXQMYLWA-------QKKNKKYGTKRAF 313
PNYT+E AW+ F+ QM +WA +K+ K Y +R
Sbjct: 238 SCPNYTYEIGAWVSFS-VLTSCLAAYLFAFAGAFQMTIWALAKHRNYKKEFKDYPRQRRS 296
Query: 314 LIPFL 318
+ PF+
Sbjct: 297 IFPFV 301
>CE20509 [I] KOG1639 Steroid reductase required for elongation of the very
long chain fatty acids
Length = 308
Score = 115 bits (288), Expect = 8e-26
Identities = 88/288 (30%), Positives = 130/288 (44%), Gaps = 36/288 (12%)
Query: 30 TYQSVLQQISKSNHNISVNRLRLSYLKEGKQVAIGPSELNDVGKKNTFDSVNEWYVKDLG 89
T +++ ++I++ ++ R L +GK +A +L+D+G + + YV+DLG
Sbjct: 28 TIKAIKKRIAQKKLKLTEERQALRVEPKGKPLA-DDQKLSDLGLSSQKAVL---YVRDLG 83
Query: 90 PQISWRLVFFIEYLGPILIHSLVYLLSLNATVRDKFHSKNVPYNDFFNKFIYRLIMV--- 146
PQI+W+ VF EY GP+ ++ L YL R F N + +
Sbjct: 84 PQIAWKTVFMAEYAGPLFVYPLFYL-------RPTFIYGQAAVNATMHPAVQIAFFAWSF 136
Query: 147 HYLKREFETLFIHSFSLETMPLFNLFKNSFHYWILNGLISLGYFGYGFPFANKTLYRVYS 206
HY KR FET FIH F TMP FNL KN +YW G+ + F N L ++
Sbjct: 137 HYAKRLFETQFIHRFGNSTMPQFNLVKNCSYYW--------GFAAFVAYFVNHPL---FT 185
Query: 207 ALKISDFRVLTALFG--LSEMFNFYIHVALRRWGDEQKRNGVTKRVPLNSG-----LFKL 259
D +V L G +SE N IH+ LR R +R+P G LF
Sbjct: 186 PPAFGDLQVYFGLAGFVISEFGNLSIHILLRNLRPAGTRE---RRIPKPDGNPLSLLFNY 242
Query: 260 LVAPNYTFESWAWMFFTXXXXXXXXXXXXXXXXXXQMYLWAQKKNKKY 307
+ PNYT+E +W+FF+ QM +WAQ K++ Y
Sbjct: 243 VSCPNYTYEVASWIFFS-IMVQSLPAIIFTTAGFAQMAIWAQGKHRNY 289
>At3g55360 [I] KOG1639 Steroid reductase required for elongation of the very
long chain fatty acids
Length = 310
Score = 88.2 bits (217), Expect = 1e-17
Identities = 60/191 (31%), Positives = 93/191 (48%), Gaps = 20/191 (10%)
Query: 86 KDLGPQISWRLVFFIEYLGPILIHSLVYLLSLNATVRDKFHSKNVPYNDFFNKFIYRLIM 145
KDLG Q+S+R +FF EYLGP+LI+ + Y + + + ++ + +Y
Sbjct: 84 KDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLG---YGEDCVIHPVQTYAMYYWCF 140
Query: 146 VHYLKREFETLFIHSFSLETMPLFNLFKNSFHYWILNGLISLGYFGYGFPFANKTLYRVY 205
HY KR ET F+H FS T P+ N+F+N +YW I+ + N LY
Sbjct: 141 -HYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAY--------YVNHPLYTPV 191
Query: 206 SALKISDFRVLTALFGL-SEMFNFYIHVALRRWGDEQKRNGVTKRVPLNSGLFKLLVAPN 264
S L++ + FGL ++ NFY H+ L+ D G ++P LF ++ N
Sbjct: 192 SDLQMK----IGFGFGLVCQVANFYCHILLKNLRDPSGAGGY--QIP-RGFLFNIVTCAN 244
Query: 265 YTFESWAWMFF 275
YT E + W+ F
Sbjct: 245 YTTEIYQWLGF 255
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.142 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,321,953
Number of Sequences: 60738
Number of extensions: 715141
Number of successful extensions: 2018
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 2003
Number of HSP's gapped (non-prelim): 5
length of query: 319
length of database: 30,389,216
effective HSP length: 106
effective length of query: 213
effective length of database: 23,950,988
effective search space: 5101560444
effective search space used: 5101560444
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)