ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1196 good J KOG2726 Translation, ribosomal structure and biogenesis Mitochondrial polypeptide chain release factor
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1196 427727 427224 -168
(168 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YLR281c [J] KOG2726 Mitochondrial polypeptide chain release factor 141 5e-34
Hs14783644 [J] KOG2726 Mitochondrial polypeptide chain release f... 72 5e-13
Hs4758744 [J] KOG2726 Mitochondrial polypeptide chain release fa... 57 1e-08
7298021 [J] KOG2726 Mitochondrial polypeptide chain release factor 52 4e-07
SPAC2F7.17 [J] KOG2726 Mitochondrial polypeptide chain release f... 50 2e-06
At5g36170 [J] KOG2726 Mitochondrial polypeptide chain release fa... 50 2e-06
Hs19923495 [J] KOG2726 Mitochondrial polypeptide chain release f... 49 2e-06
At1g56350 [J] KOG2726 Mitochondrial polypeptide chain release fa... 49 4e-06
At1g33330 [J] KOG2726 Mitochondrial polypeptide chain release fa... 47 9e-06
>YLR281c [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 155
Score = 141 bits (355), Expect = 5e-34
Identities = 72/109 (66%), Positives = 83/109 (76%), Gaps = 2/109 (1%)
Query: 19 RMLSSSIPQTIKKSKMPPRPKFTDDMERDIEEKFLHGGRGPGGQKINKCNSKVQLRHVPT 78
R +SS+ IKK+K+PPRPKFT +ME EKFLHGGRGPGGQKINKCNSKVQLRH PT
Sbjct: 8 RSISSAAVLLIKKNKLPPRPKFTPEMEAQCTEKFLHGGRGPGGQKINKCNSKVQLRHEPT 67
Query: 79 GIVVDCQATRSRDQNRKIAREKLALRLAQWENGDQPIERELAKIQWEQQ 127
GIVV+CQ TRSR+QNRK+AR KLA LA + D RE A +QW +Q
Sbjct: 68 GIVVECQETRSREQNRKLARLKLARELA--ASYDTMPSREEALLQWHRQ 114
>Hs14783644 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 166
Score = 71.6 bits (174), Expect = 5e-13
Identities = 40/87 (45%), Positives = 60/87 (67%), Gaps = 5/87 (5%)
Query: 45 ERDIEEKFLHGGRGPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKIAREKLALR 104
E ++EE+F+ G GPGGQ NK ++ V L+H+P+GIVV C TRS DQNRK+AR+ L +
Sbjct: 57 ENELEEQFVKG-HGPGGQATNKTSNCVVLKHIPSGIVVKCHQTRSVDQNRKLARKILQEK 115
Query: 105 LAQWENGD-QPI---ERELAKIQWEQQ 127
+ + NG+ P+ +RE AK + E++
Sbjct: 116 VDVFYNGENSPVHKEKREAAKKKQERK 142
>Hs4758744 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 445
Score = 57.0 bits (136), Expect = 1e-08
Identities = 37/106 (34%), Positives = 60/106 (55%), Gaps = 11/106 (10%)
Query: 6 VKTIPAVRLAITYRMLSSSIPQTIKKSKMPPRPKFTDDMERDIEEKFLH----GGRGPGG 61
V+ IP V L+ + + + I + P+P D+++ ++ K L +G GG
Sbjct: 260 VQRIPEVGLSSRMQRIHTGTMSVI----VLPQP---DEVDVKLDPKDLRIDTFRAKGAGG 312
Query: 62 QKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKIAREKLALRLAQ 107
Q +NK +S V+L H+PTG+VV+CQ RS+ +N++IA L RL Q
Sbjct: 313 QHVNKTDSAVRLVHIPTGLVVECQQERSQIKNKEIAFRVLRARLYQ 358
>7298021 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 392
Score = 52.0 bits (123), Expect = 4e-07
Identities = 34/96 (35%), Positives = 52/96 (53%), Gaps = 16/96 (16%)
Query: 25 IPQTIKKSKMP---------PRPKFTDDMERDIEEKFLH----GGRGPGGQKINKCNSKV 71
+P T K +M PRP D++ I EK L G GGQ +N +S V
Sbjct: 207 VPATEKSGRMHTSTASITVIPRPA---DIQVHIAEKDLKIETKRASGAGGQHVNTTDSAV 263
Query: 72 QLRHVPTGIVVDCQATRSRDQNRKIAREKLALRLAQ 107
++ H+PTG+ V+ Q+ RS+ +NR++A ++L RL Q
Sbjct: 264 RIVHLPTGLAVEAQSERSQLKNRELAMKRLRSRLVQ 299
>SPAC2F7.17 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 396
Score = 49.7 bits (117), Expect = 2e-06
Identities = 26/66 (39%), Positives = 40/66 (60%), Gaps = 4/66 (6%)
Query: 57 RGPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRK----IAREKLALRLAQWENGD 112
RG GGQ +N+ S V+L H+PTGI V Q +RS+ QN++ + +LA A EN
Sbjct: 265 RGAGGQHVNRTESAVRLTHIPTGITVSMQDSRSQHQNKEKAFLVLNSRLAALNAAKENEA 324
Query: 113 QPIERE 118
+ ++R+
Sbjct: 325 ERLKRK 330
>At5g36170 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 456
Score = 49.7 bits (117), Expect = 2e-06
Identities = 29/88 (32%), Positives = 44/88 (49%), Gaps = 6/88 (6%)
Query: 45 ERDIEEKFLHGGRGPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKIAREKLALR 104
E D++ F G G GGQ +NK + V++ H+PTG+ V C RS+ N+ A +L +
Sbjct: 316 EEDLDISFTRAG-GKGGQNVNKVETAVRITHIPTGVAVRCTEERSQLANKTRALIRLKAK 374
Query: 105 L-----AQWENGDQPIERELAKIQWEQQ 127
L Q + I + K +W QQ
Sbjct: 375 LMVIAEEQRATEIKEIRGDAVKAEWGQQ 402
>Hs19923495 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 380
Score = 49.3 bits (116), Expect = 2e-06
Identities = 23/64 (35%), Positives = 41/64 (63%)
Query: 58 GPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKIAREKLALRLAQWENGDQPIER 117
G GGQ +N +S V++ H+PTG+V +CQ RS+ +N+++A KL +L ++ +R
Sbjct: 248 GAGGQHVNTTDSAVRIVHLPTGVVSECQQERSQLKNKELAMTKLRAKLYSMHLEEEINKR 307
Query: 118 ELAK 121
+ A+
Sbjct: 308 QNAR 311
>At1g56350 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 426
Score = 48.5 bits (114), Expect = 4e-06
Identities = 31/83 (37%), Positives = 40/83 (47%), Gaps = 7/83 (8%)
Query: 50 EKFLHGGRGPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKIAREKLALRLAQWE 109
E+F GG G GQ N +S V++ H+PTGI CQ RS+ N+ A L RL Q E
Sbjct: 295 ERFRSGGAG--GQHANTTDSAVRIVHIPTGITATCQNERSQHSNKASAMAVLQSRLDQLE 352
Query: 110 NGDQPI-----ERELAKIQWEQQ 127
Q + L +I W Q
Sbjct: 353 MARQTAMNAQHTQSLTEISWGNQ 375
>At1g33330 [J] KOG2726 Mitochondrial polypeptide chain release factor
Length = 255
Score = 47.4 bits (111), Expect = 9e-06
Identities = 31/70 (44%), Positives = 41/70 (58%), Gaps = 4/70 (5%)
Query: 39 KFTDD--MERDIEEKFLHGGRGPGGQKINKCNSKVQLRHVPTGIVVDCQATRSRDQNRKI 96
KFTD+ M++ E F G PGGQ NK +S V+L+H+PTGIV RS+ +NR
Sbjct: 88 KFTDEELMKQCRLETFRVSG--PGGQHRNKRDSAVRLKHLPTGIVAQAVEDRSQHKNRAS 145
Query: 97 AREKLALRLA 106
A +L LA
Sbjct: 146 ALNRLRTLLA 155
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 8,155,335
Number of Sequences: 60738
Number of extensions: 337224
Number of successful extensions: 810
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 804
Number of HSP's gapped (non-prelim): 9
length of query: 168
length of database: 30,389,216
effective HSP length: 99
effective length of query: 69
effective length of database: 24,376,154
effective search space: 1681954626
effective search space used: 1681954626
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)