ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1206 good R KOG2920 General function prediction only Predicted methyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII1206 430441  431250 270  
         (270 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YLR285w [R] KOG2920 Predicted methyltransferase                       329   2e-90
SPAC8F11.09c [R] KOG2920 Predicted methyltransferase                  182   4e-46
Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA meth...    59   9e-09

>YLR285w [R] KOG2920 Predicted methyltransferase
          Length = 261

 Score =  329 bits (844), Expect = 2e-90
 Identities = 170/274 (62%), Positives = 198/274 (72%), Gaps = 20/274 (7%)

Query: 1   MSDIESLN-GGDLFAEPSDFYKPPPEPHFATYTRDDVPESSTSQQKDIKLRLVGSSPLWG 59
           MSDIESL     LF EP DF  PPP+PHFA Y R  + + S S  KDIKLRLVG+SPLWG
Sbjct: 1   MSDIESLGEAAGLFEEPEDFLPPPPKPHFAEYQRSHITKESKSDVKDIKLRLVGTSPLWG 60

Query: 60  HLLWNAGIYTAKHMDSHPEEVQDKLVLELGAAGALPTIIAGLLGARKVVSTDYPDADLIS 119
           HLLWNAGIYTA H+DSHPE ++ K VLELGAA ALP++I  L GA+ VVSTDYPD DL+ 
Sbjct: 61  HLLWNAGIYTANHLDSHPELIKGKTVLELGAAAALPSVICALNGAQMVVSTDYPDPDLMQ 120

Query: 120 NIQYNVDHNIYGGEELFKDEEKRSKQMANRKVVVEGYIWGNDYEPILKHLPQ---DQQKF 176
           NI YN+  N+    E F +            V  EGYIWGNDY P+L H+ +   +  KF
Sbjct: 121 NIDYNIKSNV---PEDFNN------------VSTEGYIWGNDYSPLLAHIEKIGNNNGKF 165

Query: 177 DLIILSDLVFNHTEHAKLFKTTKDLLRENGKALVVFSPHRPWLLENDLAFFKDCE-EFGL 235
           DLIILSDLVFNHTEH KL +TTKDLL E G+ALVVFSPHRP LLE DL FF+  + EF L
Sbjct: 166 DLIILSDLVFNHTEHHKLLQTTKDLLAEKGQALVVFSPHRPKLLEKDLEFFELAKNEFHL 225

Query: 236 KSDLIELTHWKPMFDEDEETVEIRSSIYAYYLSH 269
              LIE+ +WKPMFDEDEET+E+RS +YAYYL+H
Sbjct: 226 VPQLIEMVNWKPMFDEDEETIEVRSRVYAYYLTH 259


>SPAC8F11.09c [R] KOG2920 Predicted methyltransferase
          Length = 255

 Score =  182 bits (462), Expect = 4e-46
 Identities = 103/263 (39%), Positives = 154/263 (58%), Gaps = 23/263 (8%)

Query: 9   GGDLFAEPSDFYKPPPEPHFATYTRDDVPESSTSQQKDIKLRLVGSSPLWGHLLWNAGIY 68
           G  +F EP  F    P P    +TR  VP       ++IKLRLVGS  LW H LWN+GI 
Sbjct: 8   GFGIFEEPEGFRPSTPPPKEVLHTRVIVPNGP----EEIKLRLVGSHSLWAHYLWNSGIE 63

Query: 69  TAKHMDSHPEEVQDKLVLELGAAGALPTIIAGLLGARKVVSTDYPDADLISNIQYNVDHN 128
            A ++D +P+ V+ K VLELGA   LP+I++   GA+ VVSTDYPD  LI N+++NV   
Sbjct: 64  LANYIDKNPDTVRAKKVLELGAGAGLPSIVSAFDGAKFVVSTDYPDPALIDNLEHNV--- 120

Query: 129 IYGGEELFKDEEKRSKQMANRKVVVEGYIWGNDYEPILKHLP-QDQQKFDLIILSDLVFN 187
                       K+  ++A+ K+   GY+WG++ + ++ +   +D + FD+++LSDLVFN
Sbjct: 121 ------------KQYAEIAS-KISAVGYLWGSNIKEVMSNAGFKDNEVFDILLLSDLVFN 167

Query: 188 HTEHAKLFKTTKDLLRENGKAL--VVFSPHRPWLLENDLAFFKDCEEFGLKSDLIELTHW 245
           HTEH+KL K+ K  +  N  A+  V F+ HRP L + D+ FF   +  G + + I     
Sbjct: 168 HTEHSKLIKSCKMAIEGNPNAVVYVFFTHHRPHLAKKDMIFFDIAQSEGFQIEKILEEKR 227

Query: 246 KPMFDEDEETVEIRSSIYAYYLS 268
            PMF+ED    EIR++++ Y ++
Sbjct: 228 TPMFEEDPGAPEIRATVHGYKMT 250


>Hs17475528 [A] KOG2793 Putative N2N2-dimethylguanosine tRNA methyltransferase
          Length = 257

 Score = 58.5 bits (140), Expect = 9e-09
 Identities = 41/159 (25%), Positives = 77/159 (47%), Gaps = 22/159 (13%)

Query: 48  KLRLVGSSPLWGHLLWNAGIYTAKHMDSHPEEVQ--DKLVLELGAAGALPTIIAGLLGAR 105
           ++R+  +   +G ++W + +     ++++ ++    DK V+E+GA   L +I+A LLGA 
Sbjct: 67  EIRITEAMDCYGAVVWPSALVLCYFLETNAKQYNMVDKNVIEIGAGTGLVSIVASLLGAH 126

Query: 106 KVVSTDYPDADLISNIQYNVDHNIYGGEELFKDEEKRSKQMANRKVVVEGYIWGNDYEPI 165
            V +TD P  +L+ N+QYN+  N              +K  +     V+   WG   +  
Sbjct: 127 -VTATDLP--ELLGNLQYNISRN--------------TKMKSKHLPQVKELSWGVALD-- 167

Query: 166 LKHLPQDQQKFDLIILSDLVFNHTEHAKLFKTTKDLLRE 204
             + P+    FD I+ +D+V+ H    +L  T   L +E
Sbjct: 168 -TNFPRSSNNFDYILAADVVYAHPFLEELLITFDHLCKE 205


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.317    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,388,110
Number of Sequences: 60738
Number of extensions: 842297
Number of successful extensions: 2195
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2183
Number of HSP's gapped (non-prelim): 3
length of query: 270
length of database: 30,389,216
effective HSP length: 105
effective length of query: 165
effective length of database: 24,011,726
effective search space: 3961934790
effective search space used: 3961934790
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)