ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1393 good OUT KOG2729 Intracellular trafficking, secretion, and vesicular transport ER vesicle integral membrane protein involved in establishing cell polarity, signaling and protein degradation
r_klactIII1393 good OUT KOG2729 Posttranslational modification, protein turnover, chaperones ER vesicle integral membrane protein involved in establishing cell polarity, signaling and protein degradation
r_klactIII1393 good OUT KOG2729 Signal transduction mechanisms ER vesicle integral membrane protein involved in establishing cell polarity, signaling and protein degradation
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1393 490633 491037 135
(135 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YGL054c [OUT] KOG2729 ER vesicle integral membrane protein invol... 209 6e-55
YBR210w [OUT] KOG2729 ER vesicle integral membrane protein invol... 152 1e-37
SPAC30C2.05 [OUT] KOG2729 ER vesicle integral membrane protein i... 115 2e-26
SPAC2C4.05 [OUT] KOG2729 ER vesicle integral membrane protein in... 101 2e-22
At1g62880 [OUT] KOG2729 ER vesicle integral membrane protein inv... 89 1e-18
At1g12390 [OUT] KOG2729 ER vesicle integral membrane protein inv... 86 1e-17
Hs7661824 [OUT] KOG2729 ER vesicle integral membrane protein inv... 85 3e-17
At3g12180 [OUT] KOG2729 ER vesicle integral membrane protein inv... 82 2e-16
7298291 [OUT] KOG2729 ER vesicle integral membrane protein invol... 79 2e-15
At4g12090 [OUT] KOG2729 ER vesicle integral membrane protein inv... 73 1e-13
CE23961 [OUT] KOG2729 ER vesicle integral membrane protein invol... 62 3e-10
Hs5031639 [OUT] KOG2729 ER vesicle integral membrane protein inv... 61 4e-10
7295853 [OUT] KOG2729 ER vesicle integral membrane protein invol... 59 2e-09
Hs22062540 [OUT] KOG2729 ER vesicle integral membrane protein in... 49 2e-06
>YGL054c [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 138
Score = 209 bits (533), Expect = 6e-55
Identities = 104/134 (77%), Positives = 110/134 (81%)
Query: 1 MGVWLFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISL 60
MG WLFI AV+ NC+NLF QVHFTILYADLEADYINPIELCSKVNKLI PEAALHG +SL
Sbjct: 1 MGAWLFILAVVVNCINLFGQVHFTILYADLEADYINPIELCSKVNKLITPEAALHGALSL 60
Query: 61 LFLLNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLAXXXXXXX 120
LFLLNGYWFVFLLNL +LAYN NK Y K QLLDATEIFRTLGKHKRESF+KL
Sbjct: 61 LFLLNGYWFVFLLNLPVLAYNLNKIYNKVQLLDATEIFRTLGKHKRESFLKLGFHLLMFF 120
Query: 121 XXXXRMIMSLIAAS 134
RMIM+LIA S
Sbjct: 121 FYLYRMIMALIAES 134
>YBR210w [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 142
Score = 152 bits (384), Expect = 1e-37
Identities = 74/130 (56%), Positives = 92/130 (69%)
Query: 2 GVWLFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLL 61
G+ LF+ +I NC+N Q++FTILY DLEADYIN IELC +VN+L +PEA L FIS L
Sbjct: 5 GLSLFVTGLILNCLNSICQIYFTILYGDLEADYINSIELCKRVNRLSVPEAILQAFISAL 64
Query: 62 FLLNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLAXXXXXXXX 121
FL NGYWFVFLLN+ +LAYN +K YKK LLDAT+IFR LG+ K E F+KL
Sbjct: 65 FLFNGYWFVFLLNVPVLAYNASKVYKKTHLLDATDIFRKLGRCKIECFLKLGFYLLIFFF 124
Query: 122 XXXRMIMSLI 131
RM+ +L+
Sbjct: 125 YFYRMVTALL 134
>SPAC30C2.05 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 141
Score = 115 bits (287), Expect = 2e-26
Identities = 54/119 (45%), Positives = 77/119 (64%)
Query: 13 NCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLNGYWFVFL 72
N N+ Q+ I+++DLE DYINPI+LC+K+N L++PE H ++LL LL W +FL
Sbjct: 20 NGANMLLQIFCVIMFSDLEMDYINPIDLCNKLNDLVMPEIISHTLVTLLLLLGKKWLLFL 79
Query: 73 LNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLAXXXXXXXXXXXRMIMSLI 131
NL +L ++ N+ K +LDATEIFR LG+HKR++FIK+ M+MSLI
Sbjct: 80 ANLPLLVFHANQVIHKTHILDATEIFRQLGRHKRDNFIKVTFYLIMFFTLLYCMVMSLI 138
>SPAC2C4.05 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 134
Score = 101 bits (252), Expect = 2e-22
Identities = 43/113 (38%), Positives = 71/113 (62%)
Query: 1 MGVWLFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISL 60
+ W++ +++ C N+ Q++FT++Y+DL+ D+INPI+L K+N +LPE F +L
Sbjct: 2 VSAWIYFTSLMLTCANIMLQMYFTVMYSDLKDDFINPIDLSRKLNWYVLPEMGFQAFSAL 61
Query: 61 LFLLNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLA 113
L LL+G W FLLN+ +LA+N + D+T IF+ + ++ SF KLA
Sbjct: 62 LLLLSGAWITFLLNVPMLAWNAKMIMSNTHMHDSTTIFKDVSSRQKRSFFKLA 114
>At1g62880 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 137
Score = 89.4 bits (220), Expect = 1e-18
Identities = 44/110 (40%), Positives = 68/110 (61%), Gaps = 2/110 (1%)
Query: 4 WLFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFL 63
W+ F ++ + L V+ I ADLE DYINP + S++N ++LPE+ L GF+ + +L
Sbjct: 7 WIISFLILITLLGLI--VYQLISLADLEFDYINPYDSASRINFVVLPESILQGFLCVFYL 64
Query: 64 LNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLA 113
+ G+WF+ LL + L YN + + +KQ L+D TEIF L K++ KLA
Sbjct: 65 VTGHWFMALLCVPYLYYNFHLYSRKQHLIDVTEIFNLLDWEKKKRLFKLA 114
>At1g12390 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 126
Score = 86.3 bits (212), Expect = 1e-17
Identities = 44/110 (40%), Positives = 65/110 (59%), Gaps = 2/110 (1%)
Query: 4 WLFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFL 63
WL F + V + V+ + ADLE DYINP + S++N ++LPE + G + + +L
Sbjct: 7 WLISFFFLIALVGII--VYQLVCLADLEFDYINPYDSASRINSVVLPEFIVQGVLCVFYL 64
Query: 64 LNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLA 113
L G+WF+ LL L L YN + + K+Q L+D TEIF L K++ KLA
Sbjct: 65 LTGHWFMTLLCLPYLYYNFHLYSKRQHLVDVTEIFNLLNWEKKKRLFKLA 114
>Hs7661824 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 139
Score = 84.7 bits (208), Expect = 3e-17
Identities = 46/133 (34%), Positives = 73/133 (54%), Gaps = 6/133 (4%)
Query: 5 LFIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLL 64
+F+F+++ C +F V+F I +DLE DYIN CSK+NK ++PE H +++L L+
Sbjct: 5 VFVFSLLDCCALIFLSVYFIITLSDLECDYINARSCCSKLNKWVIPELIGHTIVTVLLLM 64
Query: 65 NGYWFVFLLNLGILAYNGNKFYK----KQQLLDATEIFR--TLGKHKRESFIKLAXXXXX 118
+ +WF+FLLNL + +N ++ + D TEI L H +E+ IKL
Sbjct: 65 SLHWFIFLLNLPVATWNIYRYIMVPSGNMGVFDPTEIHNRGQLKSHMKEAMIKLGFHLLC 124
Query: 119 XXXXXXRMIMSLI 131
MI++LI
Sbjct: 125 FFMYLYSMILALI 137
>At3g12180 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 146
Score = 82.4 bits (202), Expect = 2e-16
Identities = 43/130 (33%), Positives = 73/130 (56%), Gaps = 4/130 (3%)
Query: 3 VWLFIFAV-IANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLL 61
+W+ F V +A ++F QV I DLEADY+NP E +++N+L++PE L G + LL
Sbjct: 7 LWIVSFFVSLALVASVFYQV---ICLTDLEADYLNPFETSTRINRLVIPEFILQGSLCLL 63
Query: 62 FLLNGYWFVFLLNLGILAYNGNKFYKKQQLLDATEIFRTLGKHKRESFIKLAXXXXXXXX 121
FLL +W FL+ + + Y+ + +++ L+D TE+FR + K+ + KL
Sbjct: 64 FLLTWHWVFFLVAVPVTVYHAMLYKERRYLIDVTEVFRGISFEKKLRYTKLGFYVFLFIM 123
Query: 122 XXXRMIMSLI 131
R+ +S +
Sbjct: 124 VVFRLTLSAV 133
>7298291 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 144
Score = 78.6 bits (192), Expect = 2e-15
Identities = 45/134 (33%), Positives = 74/134 (54%), Gaps = 7/134 (5%)
Query: 6 FIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLN 65
+I A+I + +F + I + +L+ DY NPI+ C+ +N L+LPE LH F++LLFL
Sbjct: 10 YIVALIGDAFLIFFAIFHVIAFDELKTDYKNPIDQCNSLNPLVLPEYLLHIFLNLLFLFC 69
Query: 66 GYWFVFLLNLGILAYNGNKFYKK-----QQLLDATEIFR--TLGKHKRESFIKLAXXXXX 118
G WF +N+ ++AY+ ++ + L D T + + TL ++ RE +IKLA
Sbjct: 70 GEWFSLCINIPLIAYHIWRYKNRPVMSGPGLYDPTTVLKTDTLYRNMREGWIKLAVYLIS 129
Query: 119 XXXXXXRMIMSLIA 132
M+ SLI+
Sbjct: 130 FFYYIYGMVYSLIS 143
>At4g12090 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 145
Score = 72.8 bits (177), Expect = 1e-13
Identities = 34/81 (41%), Positives = 54/81 (65%), Gaps = 1/81 (1%)
Query: 20 QVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLNGYWFVFLLNLGILA 79
+V T L ADLE D INP ++ S++N+++LPE L G + L ++L G+WF+ +L+L L
Sbjct: 32 KVELTCL-ADLEFDRINPYDVSSRINRMVLPEFGLQGLLCLYYILTGHWFMAVLSLPHLF 90
Query: 80 YNGNKFYKKQQLLDATEIFRT 100
YN + K++ L D TE++ T
Sbjct: 91 YNIRLYMKREHLADVTELYNT 111
>CE23961 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 145
Score = 61.6 bits (148), Expect = 3e-10
Identities = 42/136 (30%), Positives = 66/136 (47%), Gaps = 7/136 (5%)
Query: 6 FIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLN 65
++ A+IA +F ++ I +L DY NPIE C +N+LILPE +HG ++LF+ +
Sbjct: 10 YLLALIAVGFCIFFAIYTVICVDELRTDYKNPIEQCRNLNQLILPEYIIHGTFTVLFIFS 69
Query: 66 GYWFVFLLNLGILAYNGNKFYKK-----QQLLDATEIFR--TLGKHKRESFIKLAXXXXX 118
L NL + Y+ + K+ + D T I TL R S+IKLA
Sbjct: 70 WQLISILANLPLAFYHIYTYAKRPVMSGPGIYDPTTILNRSTLSSTLRISWIKLAFYLVS 129
Query: 119 XXXXXXRMIMSLIAAS 134
MI +L+ ++
Sbjct: 130 FFYYLYAMIYTLVTSN 145
>Hs5031639 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 144
Score = 61.2 bits (147), Expect = 4e-10
Identities = 37/135 (27%), Positives = 65/135 (47%), Gaps = 7/135 (5%)
Query: 6 FIFAVIANCVNLFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLN 65
++ A++ +F + I + +L+ DY NPI+ C+ +N L+LPE +H F ++FL
Sbjct: 10 YMLALLLTAALIFFAIWHIIAFDELKTDYKNPIDQCNTLNPLVLPEYLIHAFFCVMFLCA 69
Query: 66 GYWFVFLLNLGILAYNGNKFYKK-----QQLLDATEIFRT--LGKHKRESFIKLAXXXXX 118
W LN+ +LAY+ ++ + L D T I L ++E + KLA
Sbjct: 70 AEWLTLGLNMPLLAYHIWRYMSRPVMSGPGLYDPTTIMNADILAYCQKEGWCKLAFYLLA 129
Query: 119 XXXXXXRMIMSLIAA 133
MI L+++
Sbjct: 130 FFYYLYGMIYVLVSS 144
>7295853 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 157
Score = 58.5 bits (140), Expect = 2e-09
Identities = 29/91 (31%), Positives = 52/91 (56%), Gaps = 4/91 (4%)
Query: 17 LFAQVHFTILYADLEADYINPIELCSKVNKLILPEAALHGFISLLFLLNGYWFVFLLNLG 76
L +++ + ADLE DY+N E C ++N ++P+ H + +L LL G+W +FLLNL
Sbjct: 20 LLLLIYYVLTLADLECDYLNAQECCRRLNFWVIPKFGSHALLCVLLLLGGHWVMFLLNLP 79
Query: 77 ILAYNGNKFYK-KQQLLDATEIFRTLGKHKR 106
++ + FY+ +Q D+ ++ + H R
Sbjct: 80 MVIW---LFYELHRQRRDSLGVYDPVDIHSR 107
>Hs22062540 [OUT] KOG2729 ER vesicle integral membrane protein involved in
establishing cell polarity signaling and protein
degradation
Length = 160
Score = 48.5 bits (114), Expect = 2e-06
Identities = 33/139 (23%), Positives = 62/139 (43%), Gaps = 23/139 (16%)
Query: 17 LFAQVHFTILYADLEADYINPIE----------------LCSKVNKLILPEAALHGFISL 60
+F + I + +L D+ NPI+ +C + KL++PE ++HG L
Sbjct: 21 IFFVIWHIIAFDELRTDFKNPIDQGNPARARERLKNIERICCLLRKLVVPEYSIHGLFCL 80
Query: 61 LFLLNGYWFVFLLNLGILAYNGNKFYKK-----QQLLDATEIFRT--LGKHKRESFIKLA 113
+FL W LN+ +L Y+ +++ + + + DA I L ++ES+ KLA
Sbjct: 81 MFLCAAEWVTLGLNIPLLFYHLWRYFHRPADGSEVMYDAVSIMNADILNYCQKESWCKLA 140
Query: 114 XXXXXXXXXXXRMIMSLIA 132
M+ +L++
Sbjct: 141 FYLLSFFYYLYSMVYTLVS 159
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.331 0.145 0.438
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,621,935
Number of Sequences: 60738
Number of extensions: 249616
Number of successful extensions: 884
Number of sequences better than 1.0e-05: 14
Number of HSP's better than 0.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 870
Number of HSP's gapped (non-prelim): 14
length of query: 135
length of database: 30,389,216
effective HSP length: 95
effective length of query: 40
effective length of database: 24,619,106
effective search space: 984764240
effective search space used: 984764240
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)