ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1425 good C KOG1764 Energy production and conversion 5'-AMP-activated protein kinase, gamma subunit
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1425 500622 502175 518
(518 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR214w [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 481 e-135
YGL056c [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 467 e-131
SPBC646.13 [C] KOG1764 5'-AMP-activated protein kinase gamma sub... 218 2e-56
7300717 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 56 1e-07
Hs4506061 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 55 3e-07
At1g69800 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 51 4e-06
Hs8394044 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit 50 8e-06
>YBR214w [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 527
Score = 481 bits (1237), Expect = e-135
Identities = 245/350 (70%), Positives = 279/350 (79%), Gaps = 3/350 (0%)
Query: 116 ERTGSGTGSGYVPGPNSDVPISPSLSAVDTLHSHSVSRTNSESSFDTQLSRIHTTKWQLI 175
E + T + PGP +P +V T + S LS +H KWQ I
Sbjct: 52 EESTPPTATAAAPGPGCAATPAPLRDEKPQFKLSAVPMTQTPSQC---LSCVHAQKWQHI 108
Query: 176 PLSQLVEQNKLIFIDGSISVEEAFNTLVKYNLTSLPVENSPDDIDCLTFDYNDLNSYLLL 235
PLSQL+EQNKLIF+ GSISVEEAFNTL+KY+L S+PVE+ P D++C TFDYNDLNSYLLL
Sbjct: 109 PLSQLIEQNKLIFVPGSISVEEAFNTLIKYHLNSIPVESFPGDMNCFTFDYNDLNSYLLL 168
Query: 236 VLNKITVNNPEVTRQCQLGHPVPVGEIIKLTPKNPFYKLPEQENLSTAMMILGSGVHRVA 295
VLNKITV+N ++T CQ G PVPVGE++KLTPKNPFYKLPE E+LST M ILGSGVHRVA
Sbjct: 169 VLNKITVSNKQLTADCQNGKPVPVGEMVKLTPKNPFYKLPENESLSTVMGILGSGVHRVA 228
Query: 296 ITNQENTKITGILSQRRLIKYLWDNARSFQNFEHLFQKSLKDLKIGVLDTHTKPTSRQSR 355
ITN+E TK+ GILSQRRLIKYLWDNARSF + E L SL+DL IGVL+ +KPTSRQSR
Sbjct: 229 ITNEEMTKVKGILSQRRLIKYLWDNARSFTSLEPLLNSSLQDLHIGVLNIQSKPTSRQSR 288
Query: 356 VISIQGDELLIMALFKMHTERISSIAVIDPQGNLLGNISVTDVKHITRTSQYPLLQNTCL 415
VISIQG+E LIM L+KMH ERISSIAVID QGNLLGNISVTDVKH+TRTSQYPLL TC
Sbjct: 289 VISIQGEEPLIMGLYKMHVERISSIAVIDKQGNLLGNISVTDVKHVTRTSQYPLLHKTCR 348
Query: 416 HFISVILDKRGIEMGKDSFPIFHVYPTSSLARTIAKLVATKAHRLWIVQP 465
HFISVIL+ RG+E GKDSFPIFHVYP+SSLART+AKLVATK+HRLWIVQP
Sbjct: 349 HFISVILNSRGLETGKDSFPIFHVYPSSSLARTLAKLVATKSHRLWIVQP 398
Score = 66.2 bits (160), Expect = 1e-10
Identities = 36/52 (69%), Positives = 41/52 (78%)
Query: 466 SGDTPVDSEKDKRSGKLIGVVSLTDILGLLARHQTEFKRVDPLAARRQRGSI 517
S +TP EK+ R+GKLIGVVSLTDI+ LLAR QT K VDP +ARRQRGSI
Sbjct: 474 STNTPNLFEKEYRTGKLIGVVSLTDIINLLARKQTGNKEVDPQSARRQRGSI 525
>YGL056c [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 527
Score = 467 bits (1202), Expect = e-131
Identities = 244/345 (70%), Positives = 280/345 (80%), Gaps = 3/345 (0%)
Query: 137 SPSLSAVDTLHSHSVSRTNSESSFDTQ-LSRIHTTKWQLIPLSQLVEQNKLIFIDGSISV 195
S SLS +D +S + NS S+ ++Q L +H +WQ PLS LVEQNKLIFI GSISV
Sbjct: 57 SSSLSTLDNTE-YSNNNGNSLSTLNSQNLLSVHRQEWQHTPLSNLVEQNKLIFIRGSISV 115
Query: 196 EEAFNTLVKYNLTSLPVENSPDDIDCLTFDYNDLNSYLLLVLNKITVNNPEVTRQCQLGH 255
EEAFNTLVK+ LTSLPVEN P D++CLTFDYNDLN+YLLLVLN+I V+N ++T CQ G
Sbjct: 116 EEAFNTLVKHQLTSLPVENFPGDMNCLTFDYNDLNAYLLLVLNRIKVSNDKITSDCQNGK 175
Query: 256 PVPVGEIIKLTPKNPFYKLPEQENLSTAMMILGSGVHRVAITNQENTKITGILSQRRLIK 315
VPV EI+KLTPKNPFYKLPE ENLST + ILGSGVHRVAITN E T+I GILSQRRLIK
Sbjct: 176 SVPVAEIVKLTPKNPFYKLPETENLSTVIGILGSGVHRVAITNVEMTQIKGILSQRRLIK 235
Query: 316 YLWDNARSFQNFEHLFQKSLKDLKIGVLD-THTKPTSRQSRVISIQGDELLIMALFKMHT 374
YLW+NARSF N + L SL++L IGVL+ KPT +QSRVISIQGDE LIMAL KM+
Sbjct: 236 YLWENARSFPNLKPLLDSSLEELNIGVLNAARDKPTFKQSRVISIQGDEHLIMALHKMYV 295
Query: 375 ERISSIAVIDPQGNLLGNISVTDVKHITRTSQYPLLQNTCLHFISVILDKRGIEMGKDSF 434
ERISSIAV+DPQGNL+GNISVTDVKH+TRTSQYPLL NTC HF+SVIL+ RG+E GKDSF
Sbjct: 296 ERISSIAVVDPQGNLIGNISVTDVKHVTRTSQYPLLHNTCRHFVSVILNLRGLETGKDSF 355
Query: 435 PIFHVYPTSSLARTIAKLVATKAHRLWIVQPSGDTPVDSEKDKRS 479
PIFHVYPTSSLART AKLVATK+HRLWIVQP+ + P S + S
Sbjct: 356 PIFHVYPTSSLARTFAKLVATKSHRLWIVQPNDNQPTASSEKSSS 400
Score = 67.0 bits (162), Expect = 6e-11
Identities = 33/53 (62%), Positives = 39/53 (73%)
Query: 465 PSGDTPVDSEKDKRSGKLIGVVSLTDILGLLARHQTEFKRVDPLAARRQRGSI 517
P+ TP EK+ R+GKLIGVVSLTDIL +LAR QT K +DP AR+QRG I
Sbjct: 474 PASPTPAHFEKEYRTGKLIGVVSLTDILSVLARKQTHHKEIDPQMARKQRGHI 526
>SPBC646.13 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 408
Score = 218 bits (554), Expect = 2e-56
Identities = 136/361 (37%), Positives = 206/361 (56%), Gaps = 30/361 (8%)
Query: 155 NSESSFDTQL---SRIHTTKWQLIPLSQLVEQNKLIFIDGSISVEEAFNTLVKYNLTSLP 211
NS S F Q + + + KWQ IP++ L N++ ID S+EEA + L+ +L++LP
Sbjct: 22 NSVSDFLAQFPIPTNLPSNKWQDIPVTFL-HDNEIALIDPETSMEEASSILIDRDLSALP 80
Query: 212 VENSPDDID-CLTFDYNDLNSYLLLVLNKITVNN---PEVTRQCQLGHPVPVGEIIKLTP 267
+ + + TFDY DLNS+LL+V+ N+ +V + G + E+ KL
Sbjct: 81 IVAAKGSNEIATTFDYADLNSFLLMVVGFDDFNDGRFKKVAEDIRAGKVITAYEVAKLGK 140
Query: 268 -KNPFYKLPEQENLSTAMMILGSGVHRVAITNQENTKITGILSQRRLIKYLWDNARSFQN 326
K+ F +P +L IL SG+ RVA+TN++ +++ + SQR +I++LW+N R+F +
Sbjct: 141 NKDDFITIPHTTSLGRLAEILSSGIRRVAVTNEQG-ELSFMASQRSIIRFLWNNIRAFPD 199
Query: 327 FEHLFQKSLKDLKIGVLDTHTKPTSRQSRVISIQGDELLIMALFKMHTERISSIAVIDPQ 386
E L +++ L IG D + I GD+ + AL +M+ I S+AV+D Q
Sbjct: 200 LEPLMSRTIHSLDIGSTD-----------ITCISGDQKVAAALRQMNQTGIGSLAVVDAQ 248
Query: 387 GNLLGNISVTDVKHITRTSQYPLLQNTCLHFISVILDKRGIEMGKDSFPIFHVYPTSSLA 446
LLGNIS+ DVK++TR+S LL +C HF+SVI ++GI GKDS P F++Y +S+ A
Sbjct: 249 FRLLGNISLVDVKYVTRSSSVYLLNKSCAHFLSVIKSEQGIRAGKDSAPAFNIYESSTFA 308
Query: 447 RTIAKLVATKAHRLWIVQPSGDTPVDSEKDKRS---------GKLIGVVSLTDILGLLAR 497
T+AKLVAT+ HRLW+VQ P S +L+GVVSLTDI+ +L
Sbjct: 309 FTLAKLVATQCHRLWLVQSPSCPPSPKNNAHLSPGSMGGVKVNQLLGVVSLTDIISVLYA 368
Query: 498 H 498
H
Sbjct: 369 H 369
>7300717 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 634
Score = 56.2 bits (134), Expect = 1e-07
Identities = 74/340 (21%), Positives = 138/340 (39%), Gaps = 50/340 (14%)
Query: 157 ESSFDTQLSRIHTTKWQLIPLSQLVEQNKLIFIDGSISVEEAFNTLVKYNLTSLPVENSP 216
+S + R H + LIP S KL+ D + V++AF LV + + P+ +S
Sbjct: 154 DSQIFVKFFRFHKC-YDLIPTSA-----KLVVFDTQLLVKKAFYALVYNGVRAAPLWDSE 207
Query: 217 DDIDCLTFDYNDLNSYLLLVLNKITVNNPEVTRQCQLGHPVPVGEIIKLTPKNPFYKLPE 276
D L ++ +P + + H + + P +
Sbjct: 208 KQQFVGMLTITDFIKIL-----QMYYKSPNASMEQLEEHKLDTWRSVLHNQVMPLVSIGP 262
Query: 277 QENLSTAMMIL-GSGVHRVAITNQENTKITGILSQRRLIKYLWDNARSFQNFEHLFQKSL 335
+L A+ IL S +HR+ + + + IL+ +R++++L+ ++ QKSL
Sbjct: 263 DASLYDAIKILIHSRIHRLPVIDPATGNVLYILTHKRILRFLFLYINELPKPAYM-QKSL 321
Query: 336 KDLKIGV---LDTHTKPTSRQSRVISIQGDELLIMALFKMHTERISSIAVIDPQGNLLGN 392
++LKIG ++T + TS +I AL K R+S++ ++D G L+
Sbjct: 322 RELKIGTYNNIETADETTS-------------IITALKKFVERRVSALPLVDSDGRLVDI 368
Query: 393 ISVTDVKHITRTSQYPLLQNTCLHFISVILDKRGIEMGKDSFPIFHVYPTSSLARTIAKL 452
+ DV ++ Y + + V L K + + SL + ++
Sbjct: 369 YAKFDVINLAAEKTY--------NDLDVSLRKANEHRNEWFEGVQKCNLDESLYTIMERI 420
Query: 453 VATKAHRLWIVQPSGDTPVDSEKDKRSGKLIGVVSLTDIL 492
V + HRL +V + K+IG++SL+DIL
Sbjct: 421 VRAEVHRLVVVD-------------ENRKVIGIISLSDIL 447
>Hs4506061 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 331
Score = 54.7 bits (130), Expect = 3e-07
Identities = 83/367 (22%), Positives = 154/367 (41%), Gaps = 59/367 (16%)
Query: 137 SPSLSAVDTLHSHSVSRTNSESSFDTQLSRIHTTKWQLIPLSQLVEQNKLIFIDGSISVE 196
S S AV+ H +N+ S T + H + LIP S +KL+ D S+ V+
Sbjct: 7 SDSSPAVENEHPQETPESNN--SVYTSFMKSHRC-YDLIPTS-----SKLVVFDTSLQVK 58
Query: 197 EAFNTLVKYNLTSLPVENSPDD--IDCLTFD--YNDLNSYL---LLVLNKITVNNPEVTR 249
+AF LV + + P+ +S + LT N L+ Y L+ + ++ + E R
Sbjct: 59 KAFFALVTNGVRAAPLWDSKKQSFVGMLTITDFINILHRYYKSALVQIYELEEHKIETWR 118
Query: 250 QCQLGHPVPVGEIIKLTPKNPFYKLPEQENLSTAMMILGSGVHRVAITNQENTKITGILS 309
+ L ++ ++P + ++ + +HR+ + + E+ IL+
Sbjct: 119 EVYLQDSFK--PLVCISPNASLF--------DAVSSLIRNKIHRLPVIDPESGNTLYILT 168
Query: 310 QRRLIKYLWDNARSFQNFEHLFQKSLKDLKIGVLDTHTKPTSRQSRVISIQGDELLIMAL 369
+R++K+L F E + KSL++L+IG T ++ + + +
Sbjct: 169 HKRILKFLKLFITEFPKPEFM-SKSLEELQIG--------TYANIAMVRTTTPVYVALGI 219
Query: 370 FKMHTERISSIAVIDPQGNLLGNISVTDVKHITRTSQYPLLQNTCLHFISVILDKRGIEM 429
F H R+S++ V+D +G ++ S DV ++ Y L +SV + ++
Sbjct: 220 FVQH--RVSALPVVDEKGRVVDIYSKFDVINLAAEKTYNNLD------VSV---TKALQH 268
Query: 430 GKDSFP-IFHVYPTSSLARTIAKLVATKAHRLWIVQPSGDTPVDSEKDKRSGKLIGVVSL 488
F + Y +L I +LV + HRL +V + + G+VSL
Sbjct: 269 RSHYFEGVLKCYLHETLETIINRLVEAEVHRLVVVD-------------ENDVVKGIVSL 315
Query: 489 TDILGLL 495
+DIL L
Sbjct: 316 SDILQAL 322
>At1g69800 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 447
Score = 51.2 bits (121), Expect = 4e-06
Identities = 49/237 (20%), Positives = 103/237 (42%), Gaps = 25/237 (10%)
Query: 259 VGEIIKLTPKNPFYKLPEQENLSTAMMILGS-GVHRVAITNQENTKITGILSQRRLIKYL 317
V I+K PF + + ++ + M++L + V + I ++Q ++ L
Sbjct: 208 VRTILKSFRWAPFLPVSTESSMLSVMLLLSKYRLRNVPVIKTGEPDIKNYVTQSAVVHGL 267
Query: 318 WDNARSFQNFEHLFQKSLKDLKIGVLDTHTKPTSRQSRVISIQGDELLIMALFKMHTERI 377
+ + F+H+ + DL + P + VISI+ +EL++ A +M I
Sbjct: 268 -EGCKGRDWFDHISALPISDLGL--------PFMSPNEVISIESEELILEAFKRMRDNNI 318
Query: 378 SSIAVIDP-QGNLLGNISVTDVKH-ITRTSQYPLLQNTCLHFISVILDKRGIEMGKDSFP 435
+ V++ ++GNIS+ D+++ + + + + + + + G E G + P
Sbjct: 319 GGLPVVEGLNKKIVGNISMRDIRYLLLQPEVFSNFRQLTVKSFATKIATAGEEYGL-AIP 377
Query: 436 IFHVYPTSSLARTIAKLVATKAHRLWIVQPSGDTPVDSEKDKRSGKLIGVVSLTDIL 492
P S+L I L + HR+++ +GD +L GV++L D++
Sbjct: 378 AITCRPDSTLGSVINSLASRSVHRVYVA--AGD----------ENELYGVITLRDVI 422
>Hs8394044 [C] KOG1764 5'-AMP-activated protein kinase gamma subunit
Length = 492
Score = 50.1 bits (118), Expect = 8e-06
Identities = 71/318 (22%), Positives = 132/318 (41%), Gaps = 39/318 (12%)
Query: 180 LVEQNKLIFIDGSISVEEAFNTLVKYNLTSLPVENSPDDIDCLTFDYNDLNSYLLLVLNK 239
+ +KL+ D + +++AF LV + + P+ +S D +LVL++
Sbjct: 197 MATSSKLVIFDTMLEIKKAFFALVANGVRAAPLWDSKKQSFVGMLTITDF----ILVLHR 252
Query: 240 ITVNNPEVTRQCQLGHPVPVGEIIKLTPK-NPFYKLPEQENLSTAMM-ILGSGVHRVAIT 297
+P V H + I L P + ++L A+ ++ + +HR+ +
Sbjct: 253 Y-YRSPLVQIYEIEQHKIETWREIYLQGCFKPLVSISPNDSLFEAVYTLIKNRIHRLPVL 311
Query: 298 NQENTKITGILSQRRLIKYLWDNARSFQNFEHLFQKSLKDLKIGVLDTHTKPTSRQSRVI 357
+ + + IL+ +RL+K+L L+ ++++DL IG T R V+
Sbjct: 312 DPVSGNVLHILTHKRLLKFLHIFGSLLPRPSFLY-RTIQDLGIG--------TFRDLAVV 362
Query: 358 SIQGDELLIMALFKMHTERISSIAVIDPQGNLLGNISVTDVKHITRTSQYPLLQNTCLHF 417
L + +F R+S++ V++ G ++G S DV H+ Y L +
Sbjct: 363 LETAPILTALDIFV--DRRVSALPVVNECGQVVGLYSRFDVIHLAAQQTYNHLDMS---- 416
Query: 418 ISVILDKRGIEMGKDSFPIFHVYPTSSLARTIAKLVATKAHRLWIVQPSGDTPVDSEKDK 477
+ L KR + + + P SL I ++ + HRL +V
Sbjct: 417 VGEALRKRTLCLE----GVLSCQPHESLGEVIDRIAREQVHRLVLVD------------- 459
Query: 478 RSGKLIGVVSLTDILGLL 495
+ L+GVVSL+DIL L
Sbjct: 460 ETQHLLGVVSLSDILQAL 477
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.387
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,707,105
Number of Sequences: 60738
Number of extensions: 1111386
Number of successful extensions: 3292
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 3271
Number of HSP's gapped (non-prelim): 11
length of query: 518
length of database: 30,389,216
effective HSP length: 111
effective length of query: 407
effective length of database: 23,647,298
effective search space: 9624450286
effective search space used: 9624450286
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)