ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII1445  good          O     KOG3439  Posttranslational modification, protein turnover, chaperones   Protein conjugation factor involved in autophagy

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII1445 510735  511301 189  
         (189 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YBR217w [O] KOG3439 Protein conjugation factor involved in autop...   125   5e-29
SPAC1783.06c [O] KOG3439 Protein conjugation factor involved in ...    68   9e-12
At1g54210 [O] KOG3439 Protein conjugation factor involved in aut...    51   1e-06
At3g13970 [O] KOG3439 Protein conjugation factor involved in aut...    50   2e-06
Hs4757758 [O] KOG3439 Protein conjugation factor involved in aut...    49   4e-06

>YBR217w [O] KOG3439 Protein conjugation factor involved in autophagy
          Length = 186

 Score =  125 bits (313), Expect = 5e-29
 Identities = 76/200 (38%), Positives = 105/200 (52%), Gaps = 28/200 (14%)

Query: 3   SGVLLSESETDTSEVS-------------GRQSELISGDESIKGKLEEFSAKLNELRLAD 49
           S +L SE+ET++ E S                 EL S   +++ +LE FS +L++L LA 
Sbjct: 2   SRILESENETESDESSIISTNNGTAMERSRNNQELRSSPHTVQNRLELFSRRLSQLGLAS 61

Query: 50  GNSDGGDEEESLSPDTKXXXXXXXXXXXXXXXXXXXXXXXXXIVSSVEREQLKSQVKVKI 109
             S     E+S S   +                                +  K+  K++I
Sbjct: 62  DISVDQQVEDSSSGTYEQEETIKTNAQTS---------------KQKSHKDEKNIQKIQI 106

Query: 110 RLQPIGAIPQIQPRVCQISAHQQFLALTRFLCKRLKRKHIHCYINNAFAPSLDQNIGDLW 169
           + QPIG+I Q++P VC+IS  Q F  +  FL +RLK  H++CYINN+FAPS  QNIG+LW
Sbjct: 107 KFQPIGSIGQLKPSVCKISMSQSFAMVILFLKRRLKMDHVYCYINNSFAPSPQQNIGELW 166

Query: 170 TQFKVNDELIVSYCETVAFG 189
            QFK NDELIVSYC +VAFG
Sbjct: 167 MQFKTNDELIVSYCASVAFG 186


>SPAC1783.06c [O] KOG3439 Protein conjugation factor involved in autophagy
          Length = 132

 Score = 67.8 bits (164), Expect = 9e-12
 Identities = 34/91 (37%), Positives = 55/91 (60%), Gaps = 3/91 (3%)

Query: 102 KSQVKVKIRLQPIGAIPQIQPRVCQISAHQQFLALTRFLCKRL---KRKHIHCYINNAFA 158
           K   +V +R + IG  P ++  V  I+A Q+F  +TRFL K L       +  Y+N++FA
Sbjct: 42  KENRRVNLRFKAIGRTPLLRKTVFSINASQRFEKVTRFLKKELGLPMNSSLVLYVNSSFA 101

Query: 159 PSLDQNIGDLWTQFKVNDELIVSYCETVAFG 189
           PS D+ +G+L+  F ++  L+++YC  VAFG
Sbjct: 102 PSPDEIVGNLYDNFAIDSHLLINYCINVAFG 132


>At1g54210 [O] KOG3439 Protein conjugation factor involved in autophagy
          Length = 96

 Score = 50.8 bits (120), Expect = 1e-06
 Identities = 26/95 (27%), Positives = 52/95 (54%)

Query: 95  SVEREQLKSQVKVKIRLQPIGAIPQIQPRVCQISAHQQFLALTRFLCKRLKRKHIHCYIN 154
           + E     S  KV + L+  G  P ++    +I    +F  +  FL ++L    +  Y+N
Sbjct: 2   ATESSSPSSVRKVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLHSDSLFVYVN 61

Query: 155 NAFAPSLDQNIGDLWTQFKVNDELIVSYCETVAFG 189
           +AF+P+ D+++ DL+  F  + +L+V+Y  ++A+G
Sbjct: 62  SAFSPNPDESVIDLYNNFGFDGKLVVNYACSMAWG 96


>At3g13970 [O] KOG3439 Protein conjugation factor involved in autophagy
          Length = 277

 Score = 50.1 bits (118), Expect = 2e-06
 Identities = 24/90 (26%), Positives = 51/90 (56%)

Query: 99  EQLKSQVKVKIRLQPIGAIPQIQPRVCQISAHQQFLALTRFLCKRLKRKHIHCYINNAFA 158
           E   S  K+ + L+  G  P ++    ++S   +F  +  FL ++L    +  Y+N+AF+
Sbjct: 4   ESPNSVQKIVVHLRATGGAPILKQSKFKVSGSDKFANVIDFLRRQLHSDSLFVYVNSAFS 63

Query: 159 PSLDQNIGDLWTQFKVNDELIVSYCETVAF 188
           P+ D+++ DL+  F  + +L+V+Y  ++A+
Sbjct: 64  PNPDESVIDLYNNFGFDGKLVVNYACSMAW 93


>Hs4757758 [O] KOG3439 Protein conjugation factor involved in autophagy
          Length = 140

 Score = 48.9 bits (115), Expect = 4e-06
 Identities = 26/87 (29%), Positives = 48/87 (54%), Gaps = 3/87 (3%)

Query: 106 KVKIRLQPIGAIPQIQPRVCQISAHQQFLALTRFLCKRLK---RKHIHCYINNAFAPSLD 162
           K+ I L+ +G  P ++ +   +   +    L  F+ K LK    + +  Y+N +FAPS D
Sbjct: 54  KIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPD 113

Query: 163 QNIGDLWTQFKVNDELIVSYCETVAFG 189
           Q +G L+  F  + +L++ YC++ A+G
Sbjct: 114 QEVGTLYECFGSDGKLVLHYCKSQAWG 140


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.314    0.132    0.368 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,392,556
Number of Sequences: 60738
Number of extensions: 327360
Number of successful extensions: 828
Number of sequences better than 1.0e-05: 5
Number of HSP's better than  0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 823
Number of HSP's gapped (non-prelim): 6
length of query: 189
length of database: 30,389,216
effective HSP length: 100
effective length of query: 89
effective length of database: 24,315,416
effective search space: 2164072024
effective search space used: 2164072024
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)