ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII1500  suspect: LH   K     KOG2341  Transcription                                                  TATA box binding protein (TBP)-associated factor, RNA polymerase II

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII1500 528316 527219 -366 
         (366 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR005w [K] KOG2341 TATA box binding protein (TBP)-associated fa...   300   2e-81
SPAC23G3.09 [K] KOG2341 TATA box binding protein (TBP)-associate...    66   7e-11

>YMR005w [K] KOG2341 TATA box binding protein (TBP)-associated factor RNA
           polymerase II
          Length = 388

 Score =  300 bits (769), Expect = 2e-81
 Identities = 174/391 (44%), Positives = 238/391 (60%), Gaps = 28/391 (7%)

Query: 1   MAKSPKRKNSEPEDLSSNKRSK---SFEFGKVENGLEPS-GSDFGSDLPTPF-------- 48
           MA SPK K S+   +S++   K   +    K    L P   S+    LP+P         
Sbjct: 1   MANSPK-KPSDGTGVSASDTPKYQHTVPETKPAFNLSPGKASELSHSLPSPSQIKSTAHV 59

Query: 49  -----DTMVTEQPLALPKASSPTTNLASPRNSSTPNLXXXXXXXXXXXXXXXXXNXXXXX 103
                D         LPK  SPTTNL    N  T N+                 N     
Sbjct: 60  SSTHNDAAGNTDDSVLPKNVSPTTNLRVESNGDTNNMFSSPAGLALPKKDDKKKNKGTSK 119

Query: 104 XXXXXXXXK-------PQQSDPDKLSDALLSAGVDIREEEALLSSTVARTKATGISANNQ 156
                            QQSDP+K+ D L SAG+D+REEEALL+S++  +K+   + N +
Sbjct: 120 ADSKDGKASNSSGQNAQQQSDPNKMQDVLFSAGIDVREEEALLNSSINASKSQVQTNNVK 179

Query: 157 VPSHPPFLHPKNISDFMKRIASEQNFHQDFNKNTDILGLMSTACELYMRDVITNSLILSI 216
           +P+H PFLHP+ +S++M+++  EQNF+    KN +IL +MS+ACE YMRD++TN++++S 
Sbjct: 180 IPNHLPFLHPEQVSNYMRKVGKEQNFNLTPTKNPEILDMMSSACENYMRDILTNAIVISR 239

Query: 217 HRRKGVKLNTGRRSEVSRSLRDLALRQKTQEERRVQRRIALGLEKQTTDARLDTEETQYR 276
           HRRK VK+N+GRRSEVS +LR +AL QK +EERRV++RIALGLEK+  + ++D+EET +R
Sbjct: 240 HRRKAVKINSGRRSEVSAALRAIALIQKKEEERRVKKRIALGLEKEDYENKIDSEETLHR 299

Query: 277 ASNATANLMIAGGNKKKYSWLTAG-SKSSSTDLKNQGNVSSAVAARGEMGIKYREAREEP 335
           ASN TA L    G+KK+Y WLT+  +K +S   K+ G V+S + ARGE G+K+REAREEP
Sbjct: 300 ASNVTAGL--RAGSKKQYGWLTSSVNKPTSLGAKSSGKVASDITARGESGLKFREAREEP 357

Query: 336 GIVMRDLLLALENRRVGVNNVITKGYARIRD 366
           GIVMRDLL ALENRR  V  +I+KGYA+IRD
Sbjct: 358 GIVMRDLLFALENRRNSVQTIISKGYAKIRD 388


>SPAC23G3.09 [K] KOG2341 TATA box binding protein (TBP)-associated factor RNA
           polymerase II
          Length = 365

 Score = 66.2 bits (160), Expect = 7e-11
 Identities = 68/297 (22%), Positives = 125/297 (41%), Gaps = 51/297 (17%)

Query: 115 QSDPDKLSDALLSAGVDIREEEALLSSTVARTKATGISA--NNQVPSHPPFLHPKNISDF 172
           + + D+L DAL+S G+ ++EEE  LS++     +    A           FL   N    
Sbjct: 75  RQEADQLQDALISCGIQLKEEELNLSTSFYDPSSLNTFALTTEDRSRKSDFL---NSFVL 131

Query: 173 MKRIASEQNFHQDFNKNTDILGLMSTACELYMRDVITNSLILSIHRRKGVKLNTGRR-SE 231
           M+ +++  N H+  + ++DI  L+S A   Y+ +++   ++ S HR   +  +  ++   
Sbjct: 132 MQTVSNIVNLHRLKSMDSDIHALISMAVRDYLANLLQKMIVESHHRTSQLHTDNYKQVDN 191

Query: 232 VSRSLRDLALRQKTQEERRVQRRIALGLEKQTTDARLD---------------------- 269
           V ++L + A ++   EER   RR  L + +   +ARL                       
Sbjct: 192 VRQTLANFAYKEYESEER---RRTVLNIRRAEHEARLAELNSASTNEEGSSRRRKEQSSS 248

Query: 270 ------TEETQYRASNATANLMIAG---GNKKKYSWLTAGSKSSSTDLKNQGNV----SS 316
                 +E+ Q R +NATA++M         KKYSW+       +  +     +    S+
Sbjct: 249 AAAKNISEDAQNRMTNATASIMAGSALPSGGKKYSWMATDMTPMTPAVGGGFGIRKKDSN 308

Query: 317 AVAARGEMGIKYREAREEPGIVMRDLLLALENRRVGVNNVITKG-------YARIRD 366
           ++      G+   +  E+  I +RD L  LE  R G   +  +G       Y R++D
Sbjct: 309 SLKPSSRDGVLPLQQEEKGIITIRDALAVLEMDREGAGRIFGRGAKAMMRAYIRLKD 365


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.310    0.127    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,968,055
Number of Sequences: 60738
Number of extensions: 749702
Number of successful extensions: 2120
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2115
Number of HSP's gapped (non-prelim): 2
length of query: 366
length of database: 30,389,216
effective HSP length: 108
effective length of query: 258
effective length of database: 23,829,512
effective search space: 6148014096
effective search space used: 6148014096
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)