ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1527 good L KOG0981 Replication, recombination and repair DNA topoisomerase I
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1527 538881 536527 -785
(785 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL006c [L] KOG0981 DNA topoisomerase I 961 0.0
SPBC1703.14c [L] KOG0981 DNA topoisomerase I 674 0.0
7293054 [L] KOG0981 DNA topoisomerase I 447 e-125
Hs11225260 [L] KOG0981 DNA topoisomerase I 432 e-121
At5g55300 [L] KOG0981 DNA topoisomerase I 425 e-118
At5g55310 [L] KOG0981 DNA topoisomerase I 418 e-116
Hs16418461 [L] KOG0981 DNA topoisomerase I 412 e-114
CE12284 [L] KOG0981 DNA topoisomerase I 402 e-112
CE12282 [L] KOG0981 DNA topoisomerase I 401 e-111
>YOL006c [L] KOG0981 DNA topoisomerase I
Length = 769
Score = 961 bits (2483), Expect = 0.0
Identities = 462/646 (71%), Positives = 530/646 (81%), Gaps = 1/646 (0%)
Query: 140 KESSEDFKWWENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEE 199
KE E++KWWE + EDDTIKW TLKHNGVIFP Y+PLP+H+KLYY+GKPVDLPPQAEE
Sbjct: 125 KEEEEEYKWWEK-ENEDDTIKWVTLKHNGVIFPPPYQPLPSHIKLYYDGKPVDLPPQAEE 183
Query: 200 VAGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFEYFQV 259
VAGFFAA+LESDH K P FQKNF +DFLQVLKE+GG NG++IKEF + DFTK+F+YFQ+
Sbjct: 184 VAGFFAALLESDHAKNPVFQKNFFNDFLQVLKESGGPLNGIEIKEFSRCDFTKMFDYFQL 243
Query: 260 QKEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNG 319
QKEQKKQL+ YKFCE+DGR EQVGNF++EPP LFRGRGAHPK G
Sbjct: 244 QKEQKKQLTSQEKKQIRLEREKFEEDYKFCELDGRREQVGNFKVEPPDLFRGRGAHPKTG 303
Query: 320 KLKRRVYPEDVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYVRFAA 379
KLKRRV PED+VLNL K+AP+P P+GHKWGEIRHDNTVQWL+MWRENI NSFKYVR AA
Sbjct: 304 KLKRRVNPEDIVLNLSKDAPVPPAPEGHKWGEIRHDNTVQWLAMWRENIFNSFKYVRLAA 363
Query: 380 NSTLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGG 439
NS+LKG SD+KKFEKAR LK +ID +R DY +KS L+RQ AVA YLIDVFALRAGG
Sbjct: 364 NSSLKGQSDYKKFEKARQLKSYIDAIRRDYTRNLKSKVMLERQKAVAIYLIDVFALRAGG 423
Query: 440 EKADDEADTVGCCSLRYEHVTLRPPNTVVFDFLGKDSIRYYQEVEVDAQVFKNLKIFKRP 499
EK++DEADTVGCCSLRYEHVTL+PPNTV+FDFLGKDSIR+YQEVEVD QVFKNL IFKRP
Sbjct: 424 EKSEDEADTVGCCSLRYEHVTLKPPNTVIFDFLGKDSIRFYQEVEVDKQVFKNLTIFKRP 483
Query: 500 PSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNEGTVAEKIL 559
P PGHQLFDRLDPSILNK+LQNYMPGLTAKVFRTYNASKTMQDQLDLIPN+G+VAEKIL
Sbjct: 484 PKQPGHQLFDRLDPSILNKYLQNYMPGLTAKVFRTYNASKTMQDQLDLIPNKGSVAEKIL 543
Query: 560 RYNAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKVRYKRAILQLDKSERKKNQ 619
+YNAANRTVAILCNHQRTVTKGHA +V+KA RI+ELEWQK+R KRAILQLDK KK
Sbjct: 544 KYNAANRTVAILCNHQRTVTKGHAQTVEKANNRIQELEWQKIRCKRAILQLDKDLLKKEP 603
Query: 620 NYFKEIDDLTKEEELTIHXXXXXXXXXXXXXXXXXXXXXXXXXXXDPIPDSQLLEWLAKV 679
YF+EIDDLTKE+E TIH + +P+SQL EWL KV
Sbjct: 604 KYFEEIDDLTKEDEATIHKRIIDREIEKYQRKFVRENDKRKFEKEELLPESQLKEWLEKV 663
Query: 680 DDLEKLYERESTTGKIELRESLQNLEKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTS 739
D+ ++ +E+E TG++EL+ S ++EK+K VEKLE+RI S+QLKDKE+NS V+LGTS
Sbjct: 664 DEKKQEFEKELKTGEVELKSSWNSVEKIKAQVEKLEQRIQTSSIQLKDKEENSQVSLGTS 723
Query: 740 KINYIDPRLSVAFCKKFDVPIEKVFTKTLRDKFRWAIESADENWTF 785
KINYIDPRLSV FCKK+DVPIEK+FTKTLR+KF+WAIES DENW F
Sbjct: 724 KINYIDPRLSVVFCKKYDVPIEKIFTKTLREKFKWAIESVDENWRF 769
>SPBC1703.14c [L] KOG0981 DNA topoisomerase I
Length = 814
Score = 674 bits (1738), Expect = 0.0
Identities = 349/676 (51%), Positives = 452/676 (66%), Gaps = 22/676 (3%)
Query: 118 NLADIASETPFQSSA--RASSIELKESSEDFKWWENGDKEDDTIKWTTLKHNGVIFPSTY 175
+L SE P +S S + ++ ED+KWW + + DDT KWTTL+HNGVIF Y
Sbjct: 151 SLTPYKSELPSGASTTQNRSPNDEEDEDEDYKWWTS-ENIDDTQKWTTLEHNGVIFAPPY 209
Query: 176 EPLPAHVKLYYNGKPVDLPPQAEEVAGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGG 235
EPLP +VKL Y+G PV+LPP+AEEVAGF+AA+LE+DH K P FQ NF DFL+V E
Sbjct: 210 EPLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNF 269
Query: 236 ARNGVQIKEFGKLDFTKIFEYFQVQKEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRV 295
N IKEF K DFT++F +F+ ++E+KK + YK+C +DGR
Sbjct: 270 NHN---IKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRK 326
Query: 296 EQVGNFRIEPPGLFRGRGAHPKNGKLKRRVYPEDVVLNLDKEAPIPEPPKGHKWGEIRHD 355
E+VGNFRIEPPGLFRGRG+HPK G LKRRVYPE + +N+ + P+PEP GH+W E++HD
Sbjct: 327 EKVGNFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPEPLPGHQWAEVKHD 386
Query: 356 NTVQWLSMWRENISNSFKYVRFAANSTLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKS 415
NTV WL+ W ENI+N+ KYV AA S+LKG SD KK+EK+R LKD+ID +R Y+ +KS
Sbjct: 387 NTVTWLATWHENINNNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKS 446
Query: 416 PKTLDRQMAVATYLIDVFALRAGGEKADDEADTVGCCSLRYEHVTLRPPNTVVFDFLGKD 475
T++RQ A YLIDVFALRAG EK +DEADTVGCCSLRYEHVTL+PP TVVFDFLGKD
Sbjct: 447 ELTVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKD 506
Query: 476 SIRYYQEVEVDAQVFKNLKIFKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTY 535
SIRYY EVEVD QVFKNLKIFKRPP G +FDRL + LNK+L + M GL+AKVFRTY
Sbjct: 507 SIRYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTY 566
Query: 536 NASKTMQDQLDLIPNEGTVAEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERIEE 595
NAS TM ++L +P T+A+KIL YN ANRTVAILCNHQR+VTK H +++ ERI+
Sbjct: 567 NASYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKA 626
Query: 596 LEWQKVRYKRAILQLDKSERKKNQNYFKEIDDLTKEEELT----IHXXXXXXXXXXXXXX 651
L++Q++R ++ +L L+ K L KEE +T +
Sbjct: 627 LQYQRMRLRKMMLNLEPKLAKSKPEL------LAKEEGITDSWIVKHHETLYELEKEKIK 680
Query: 652 XXXXXXXXXXXXXDP---IPDSQLLEWLAKVDDLEKLYERESTTGKIELRESLQNLEKLK 708
DP +P+S+L L D+L+K + E + K++ S ++E+L+
Sbjct: 681 KKFDRENEKLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRS--SMEQLE 738
Query: 709 QNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKVFTKTL 768
+ + KL +RI Q+ DK++N T ALGTSKINYIDPRL+ +F K+ DVPIEK+F+KT+
Sbjct: 739 KRLNKLNERINVMRTQMIDKDENKTTALGTSKINYIDPRLTYSFSKREDVPIEKLFSKTI 798
Query: 769 RDKFRWAIES-ADENW 783
RDKF WA ++ D W
Sbjct: 799 RDKFNWAADTPPDWKW 814
>7293054 [L] KOG0981 DNA topoisomerase I
Length = 974
Score = 447 bits (1149), Expect = e-125
Identities = 237/507 (46%), Positives = 312/507 (60%), Gaps = 17/507 (3%)
Query: 141 ESSEDFKWWENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEEV 200
E E ++WWE +K D +KW+TL+H G +F YE +P +V+ YY+GKP++L + EE
Sbjct: 423 EEEEVWRWWEE-EKRADGVKWSTLEHKGPVFAPRYERVPRNVRFYYDGKPLELSEETEEA 481
Query: 201 AGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFEYFQVQ 260
A F+A +L D+ F NF DF +++ R IK+F K +F ++F YFQ +
Sbjct: 482 ATFYAKMLNHDYCTKEVFNNNFFKDF----RKSMTPREREIIKDFRKCNFQEMFNYFQAE 537
Query: 261 KEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNGK 320
E++K S + FC IDG E++GNFR+EPPGLFRGRG HPK G
Sbjct: 538 SEKRKAASKEEKLIKKNENEALMKEFGFCMIDGHKEKIGNFRLEPPGLFRGRGEHPKMGM 597
Query: 321 LKRRVYPEDVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYVRFAAN 380
+KRR+ DV +N K++ +P PP G +W E+RHDNTV WL+ W EN+ KY+ +
Sbjct: 598 IKRRIQASDVSINCGKDSKVPSPPPGSRWKEVRHDNTVTWLASWIENVQGQVKYIMLNPS 657
Query: 381 STLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGGE 440
S LKG D K+E AR L IDK+R Y+ + KS + RQ AVA Y ID ALRAG E
Sbjct: 658 SKLKGEKDHIKYETARRLDKVIDKIRATYRDEWKSKEMRVRQRAVALYFIDKLALRAGNE 717
Query: 441 KADDEADTVGCCSLRYEHVTLR-----PPNTVVFDFLGKDSIRYYQEVEVDAQVFKNLKI 495
K +D+ADTVGCCSLR EHV L N VVFDF GKDSIRYY EVEV+ +VFKNL++
Sbjct: 718 KDEDQADTVGCCSLRVEHVQLHKELNGKENVVVFDFPGKDSIRYYNEVEVEKRVFKNLEL 777
Query: 496 FKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPN-EGTV 554
F G LFDRL+ +LN+HL+ M GLTAKVFRTYNASKT+Q QLDL+ + TV
Sbjct: 778 FMEHKK-EGDDLFDRLNTQVLNEHLKELMEGLTAKVFRTYNASKTLQSQLDLLTDPSATV 836
Query: 555 AEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERI----EELEWQKVRYKRAILQL 610
EK+L YN ANR VAILCNHQR+V K H S++ E+I E +E + Y +
Sbjct: 837 PEKLLAYNRANRAVAILCNHQRSVPKSHEKSMENLKEKIKAKREAIEKCESEYHSRDEKK 896
Query: 611 DKS-ERKKNQNYFKEIDDLTKEEELTI 636
K ER ++Q E+ + ++E TI
Sbjct: 897 GKQLERLRDQLKKLELQETDRDENKTI 923
Score = 102 bits (254), Expect = 2e-21
Identities = 50/103 (48%), Positives = 70/103 (67%), Gaps = 1/103 (0%)
Query: 683 EKLYERESTTGKIELRESLQNLEKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKIN 742
EK+ + K E ++ +K KQ +E+L ++ LQ D+++N T+ALGTSK+N
Sbjct: 873 EKIKAKREAIEKCESEYHSRDEKKGKQ-LERLRDQLKKLELQETDRDENKTIALGTSKLN 931
Query: 743 YIDPRLSVAFCKKFDVPIEKVFTKTLRDKFRWAIESADENWTF 785
Y+DPR+SVA+CKK DVPIEK+F KT R KF WA+ ADEN+ F
Sbjct: 932 YLDPRISVAWCKKHDVPIEKIFNKTQRTKFLWAVHMADENYRF 974
>Hs11225260 [L] KOG0981 DNA topoisomerase I
Length = 765
Score = 432 bits (1112), Expect = e-121
Identities = 229/473 (48%), Positives = 292/473 (61%), Gaps = 13/473 (2%)
Query: 140 KESSEDFKWWENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEE 199
KE + +KWWE ++ + IKW L+H G +F YEPLP +VK YY+GK + L P+AEE
Sbjct: 197 KEEEQKWKWWEE-ERYPEGIKWKFLEHKGPVFAPPYEPLPENVKFYYDGKVMKLSPKAEE 255
Query: 200 VAGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFEYFQV 259
VA FFA +L+ ++ F+KNF D+ KE + I K DFT++ +YF+
Sbjct: 256 VATFFAKMLDHEYTTKEIFRKNFFKDW---RKEMTNEEKNI-ITNLSKCDFTQMSQYFKA 311
Query: 260 QKEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNG 319
Q E +KQ+S Y FC +D E++ NF+IEPPGLFRGRG HPK G
Sbjct: 312 QTEARKQMSKEEKLKIKEENEKLLKEYGFCIMDNHKERIANFKIEPPGLFRGRGNHPKMG 371
Query: 320 KLKRRVYPEDVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYVRFAA 379
LKRR+ PED+++N K+A +P PP GHKW E+RHDN V WL W ENI S KY+
Sbjct: 372 MLKRRIMPEDIIINCSKDAKVPSPPPGHKWKEVRHDNKVTWLVSWTENIQGSIKYIMLNP 431
Query: 380 NSTLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGG 439
+S +KG D++K+E AR LK +DK+R Y+ KS + RQ AVA Y ID ALRAG
Sbjct: 432 SSRIKGEKDWQKYETARRLKKCVDKIRNQYREDWKSKEMKVRQRAVALYFIDKLALRAGN 491
Query: 440 EKADDE-ADTVGCCSLRYEHVTLRP-----PNTVVFDFLGKDSIRYYQEVEVDAQVFKNL 493
EK + E ADTVGCCSLR EH+ L P V FDFLGKDSIRYY +V V+ +VFKNL
Sbjct: 492 EKEEGETADTVGCCSLRVEHINLHPELDGQEYVVEFDFLGKDSIRYYNKVPVEKRVFKNL 551
Query: 494 KIFKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQL-DLIPNEG 552
++F P LFDRL+ ILNKHLQ+ M GLTAKVFRTYNAS T+Q QL +L +
Sbjct: 552 QLFMENKQ-PEDDLFDRLNTGILNKHLQDLMEGLTAKVFRTYNASITLQQQLKELTAPDE 610
Query: 553 TVAEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKVRYKR 605
+ KIL YN ANR VAILCNHQR K S+ +I+ + Q +R
Sbjct: 611 NIPAKILSYNRANRAVAILCNHQRAPPKTFEKSMMNLQTKIDAKKEQLADARR 663
Score = 99.8 bits (247), Expect = 1e-20
Identities = 42/82 (51%), Positives = 65/82 (79%)
Query: 704 LEKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKV 763
+E K+ V++LE+++ +Q D+E+N +ALGTSK+NY+DPR++VA+CKK+ VPIEK+
Sbjct: 684 VESKKKAVQRLEEQLMKLEVQATDREENKQIALGTSKLNYLDPRITVAWCKKWGVPIEKI 743
Query: 764 FTKTLRDKFRWAIESADENWTF 785
+ KT R+KF WAI+ ADE++ F
Sbjct: 744 YNKTQREKFAWAIDMADEDYEF 765
>At5g55300 [L] KOG0981 DNA topoisomerase I
Length = 916
Score = 425 bits (1092), Expect = e-118
Identities = 233/492 (47%), Positives = 312/492 (63%), Gaps = 18/492 (3%)
Query: 132 ARASSIELKESSEDFKWWENGDKE----DDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYN 187
+RA + K+ ++D K+ + D KWTTL HNGVIFP Y+P +K+ Y
Sbjct: 338 SRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKP--HGIKILYK 395
Query: 188 GKPVDLPPQAEEVAGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGK 247
GKPVDL + EEVA FA + E+D+ P F++NF +D+ ++L G ++ +Q +
Sbjct: 396 GKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLL----GKKHVIQ--KLDD 449
Query: 248 LDFTKIFEYFQVQKEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPG 307
DFT I+E+ +KE+KKQ+S Y + +DG E++GNFR+EPPG
Sbjct: 450 CDFTPIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPG 509
Query: 308 LFRGRGAHPKNGKLKRRVYPEDVVLNLDKEAPIPEPP-KGHKWGEIRHDNTVQWLSMWRE 366
LFRGRG HPK GKLK+R++P ++ LN+ K APIPE P G +W E++HDNTV WL+ W +
Sbjct: 510 LFRGRGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWAD 569
Query: 367 NIS-NSFKYVRFAANSTLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAV 425
I+ FKYV A S+LKG+SD +K+EKAR L DHID +RT Y + RQ+AV
Sbjct: 570 PINPKEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAV 629
Query: 426 ATYLIDVFALRAGGEKADDEADTVGCCSLRYEHVTLRPPNTVVFDFLGKDSIRYYQEVEV 485
ATYLID ALRAG EK DDEADTVGCC+L+ +V PPN + FDFLGKDSI+Y VEV
Sbjct: 630 ATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEV 689
Query: 486 DAQVFKNLKIFKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQL 545
+ V+K + F+ S LFD LD S LN HL+ +PGLTAKVFRTYNAS T+ + L
Sbjct: 690 EPLVYKAIGQFQAGKS-KTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEML 748
Query: 546 DLIPNEGTVAEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKVRYKR 605
+G V +KI+ Y AN+ VAI+CNHQRTV+K H + ++K T RIEEL K K
Sbjct: 749 SQETKDGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEEL---KEVLKE 805
Query: 606 AILQLDKSERKK 617
LD++++ K
Sbjct: 806 LKTNLDRAKKGK 817
Score = 82.8 bits (203), Expect = 2e-15
Identities = 50/128 (39%), Positives = 78/128 (60%), Gaps = 20/128 (15%)
Query: 670 SQLLEWLAKVDDLEKLYERESTT------GKIELRES----LQNLE------KLKQNVEK 713
+Q+ + A++++L+++ + T GK L S +++LE K+ Q K
Sbjct: 787 AQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAK 846
Query: 714 LEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKVFTKTLRDKFR 773
+EK + KED TVALGTSKINY+DPR++VA+CK+ +VPIEK+FTK+L +KF
Sbjct: 847 IEKM----ERDMHTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFTKSLLEKFA 902
Query: 774 WAIESADE 781
WA++ E
Sbjct: 903 WAMDVEPE 910
>At5g55310 [L] KOG0981 DNA topoisomerase I
Length = 917
Score = 418 bits (1074), Expect = e-116
Identities = 232/511 (45%), Positives = 315/511 (61%), Gaps = 17/511 (3%)
Query: 110 SNEDDEDKNLADIASETPFQSSARASSIELKESSEDFKWWENGDK---EDDTIKWTTLKH 166
S+ + + A +S SS+R + + + +D K+ ++ D KW TL+H
Sbjct: 315 SDSSNNKSSSAKPSSSKMIASSSRTIAQKPNKWVKDSKYSKSSKSLPSGDGQKKWKTLQH 374
Query: 167 NGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEEVAGFFAAILESDHVKIPTFQKNFLHDF 226
NGVIFP Y+ VK+ + GKPVDL P+ EEVA FA + E+++ K P F++NF +D+
Sbjct: 375 NGVIFPPPYKR--HGVKILFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDW 432
Query: 227 LQVLKENGGARNGVQIKEFGKLDFTKIFEYFQVQKEQKKQLSPXXXXXXXXXXXXXXXPY 286
++L +N IK DFT I+E++ +KE KKQ++ Y
Sbjct: 433 RKLLGKNH------MIKSLDDCDFTPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKY 486
Query: 287 KFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNGKLKRRVYPEDVVLNLDKEAPIPEPP-K 345
+ +DG E++GNFR+EPPGLFRGRG HPK GKLK+R+ P D+ +N+ KEAPIPE P
Sbjct: 487 MWAVLDGVRERIGNFRVEPPGLFRGRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIP 546
Query: 346 GHKWGEIRHDNTVQWLSMWRENIS-NSFKYVRFAANSTLKGMSDFKKFEKARMLKDHIDK 404
G +W E++HDNTV WL+ W + I+ FKYV AA+S+LKG SD +K+EKAR L +HI
Sbjct: 547 GERWKEVKHDNTVTWLAFWSDPINPKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGS 606
Query: 405 VRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGGEKADDEADTVGCCSLRYEHVTLRPP 464
+R Y + RQ+AVATYLID ALRAG EK DDEADTVGCC+L+ +V PP
Sbjct: 607 IRAAYTKDFNNKDVTKRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPP 666
Query: 465 NTVVFDFLGKDSIRYYQEVEVDAQVFKNLKIFKRPPSGPGHQLFDRLDPSILNKHLQNYM 524
N + FDFLGKDSI+Y VEV+ V+K + F+ S LFD LD S LN HL+ M
Sbjct: 667 NKLKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQAGKS-KTDDLFDELDTSKLNTHLKELM 725
Query: 525 PGLTAKVFRTYNASKTMQDQLDLIPNEGTVAEKILRYNAANRTVAILCNHQRTVTKGHAS 584
GLTAKVFRTYNAS T+ L +G V EK++ Y AN+ VAI+CNHQRTV+K H +
Sbjct: 726 AGLTAKVFRTYNASITLDLMLSKETRDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGA 785
Query: 585 SVQKATERIEELEWQKVRYKRAILQLDKSER 615
V+K +IEEL Q K + LD++++
Sbjct: 786 QVEKLAVKIEELREQ---IKELNIDLDRAKK 813
Score = 82.8 bits (203), Expect = 2e-15
Identities = 37/73 (50%), Positives = 54/73 (73%)
Query: 705 EKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKVF 764
E L++ + + + +I ++ KED TVALGTSKINY+DPR++VA+CK+ DVPIEK+F
Sbjct: 832 EALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINYMDPRITVAWCKRHDVPIEKIF 891
Query: 765 TKTLRDKFRWAIE 777
K+L KF WA++
Sbjct: 892 NKSLLAKFAWAMD 904
>Hs16418461 [L] KOG0981 DNA topoisomerase I
Length = 601
Score = 412 bits (1059), Expect = e-114
Identities = 223/475 (46%), Positives = 291/475 (60%), Gaps = 14/475 (2%)
Query: 149 WENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEEVAGFFAAIL 208
WE +K +D +KW L+H G F YEPLP V+ +Y G+PV L AEEVA F+ +L
Sbjct: 42 WEK-EKHEDGVKWRQLEHKGPYFAPPYEPLPDGVRFFYEGRPVRLSVAAEEVATFYGRML 100
Query: 209 ESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFEYFQVQKEQKKQLS 268
+ ++ F+KNF +D+ ++ IK K DFT+I YF + +K LS
Sbjct: 101 DHEYTTKEVFRKNFFNDW----RKEMAVEEREVIKSLDKCDFTEIHRYFVDKAAARKVLS 156
Query: 269 PXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNGKLKRRVYPE 328
+ +C +DG E++GNF+IEPPGLFRGRG HPK G LKRR+ PE
Sbjct: 157 REEKQKLKEEAEKLQQEFGYCILDGHQEKIGNFKIEPPGLFRGRGDHPKMGMLKRRITPE 216
Query: 329 DVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYVRFAANSTLKGMSD 388
DVV+N +++ IPEPP GH+W E+R DNTV WL+ W E++ NS KY+ S LKG +
Sbjct: 217 DVVINCSRDSKIPEPPAGHQWKEVRSDNTVTWLAAWTESVQNSIKYIMLNPCSKLKGETA 276
Query: 389 FKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGGEKADDE-AD 447
++KFE AR L+ +D++R+ Y++ KS + RQ AVA Y ID ALRAG EK D E AD
Sbjct: 277 WQKFETARRLRGFVDEIRSQYRADWKSREMKTRQRAVALYFIDKLALRAGNEKEDGEAAD 336
Query: 448 TVGCCSLRYEHVTLRP-----PNTVVFDFLGKDSIRYYQEVEVDAQVFKNLKIFKRPPSG 502
TVGCCSLR EHV L P + V FDFLGKD IRYY V V+ V+KNL++F
Sbjct: 337 TVGCCSLRVEHVQLHPEADGCQHVVEFDFLGKDCIRYYNRVPVEKPVYKNLQLFME-NKD 395
Query: 503 PGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPN-EGTVAEKILRY 561
P LFDRL + LNKHLQ M GLTAKVFRTYNAS T+Q+QL + E ++A KIL Y
Sbjct: 396 PRDDLFDRLTTTSLNKHLQELMDGLTAKVFRTYNASITLQEQLRALTRAEDSIAAKILSY 455
Query: 562 NAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKVRYKRAILQLDKSERK 616
N ANR VAILCNHQR S+Q +I+ + ++V RA L+ ++E K
Sbjct: 456 NRANRVVAILCNHQRATPSTFEKSMQNLQTKIQAKK-EQVAEARAELRRARAEHK 509
Score = 99.8 bits (247), Expect = 1e-20
Identities = 42/82 (51%), Positives = 66/82 (80%)
Query: 704 LEKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKV 763
LEK ++ +EKL++++ S+Q DKE+N VALGTSK+NY+DPR+S+A+CK+F VP+EK+
Sbjct: 520 LEKKRRLLEKLQEQLAQLSVQATDKEENKQVALGTSKLNYLDPRISIAWCKRFRVPVEKI 579
Query: 764 FTKTLRDKFRWAIESADENWTF 785
++KT R++F WA+ A E++ F
Sbjct: 580 YSKTQRERFAWALAMAGEDFEF 601
>CE12284 [L] KOG0981 DNA topoisomerase I
Length = 734
Score = 402 bits (1034), Expect = e-112
Identities = 228/564 (40%), Positives = 320/564 (56%), Gaps = 44/564 (7%)
Query: 110 SNEDDEDKNLADIASET---------------PFQSSARASS--------IELKESSED- 145
S+EDD++++ D++ E P S A ++ +E ED
Sbjct: 113 SSEDDDEESEGDVSEEDVKPQKRKSKGAEKSKPSTSKKDAGGKKEPPKKKVKKEEDIEDI 172
Query: 146 FKWWENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPPQAEEVAGFFA 205
++WW+ +K+ +KW +L+H G +F Y PLP+HV Y G+ + L + EE+A F+A
Sbjct: 173 WEWWKE-EKKPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTLETEEIAQFYA 231
Query: 206 AILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFEYFQVQKEQKK 265
+L+ ++ F KNF+ D+ +V+ R I + K DF I Y + Q+E +K
Sbjct: 232 GVLDHEYSTKEAFNKNFMKDWRKVMTVEERER----IHDLKKCDFRAIDAYQKEQREIRK 287
Query: 266 QLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAHPKNGKLKRRV 325
++ Y IDG ++V NFRIEPPG+FRGRG HPK G +K+R+
Sbjct: 288 AMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGHPKMGLIKKRI 347
Query: 326 YPEDVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYVRFAANSTLKG 385
PEDV++N K+ IP+PP GHKW E+RHDNTV WL W E++ KY+ +S +KG
Sbjct: 348 MPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYIMLNPSSKIKG 407
Query: 386 MSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFALRAGGEK-ADD 444
DF+K+E AR LK I +R Y KS + RQ A A Y ID ALRAG EK D+
Sbjct: 408 EKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLALRAGNEKDVDE 467
Query: 445 EADTVGCCSLRYEHVTL------------RPPNTVVFDFLGKDSIRYYQEVEVDAQVFKN 492
ADTVGCCSLR EH+ L V FDFLGKDSIRY+ V V+ +V+KN
Sbjct: 468 AADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNRVSVEKRVYKN 527
Query: 493 LKIFKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQDQLDLIPN-E 551
LKIF P LFDRLD + LN HL++ M GLT KVFRTYNAS T+Q+QL + N +
Sbjct: 528 LKIFME-GKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQEQLIKLTNPK 586
Query: 552 GTVAEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKVRYKRAILQLD 611
VA KIL YN ANR VAILCNHQR V+KG S+QK ++I++ + + + A+
Sbjct: 587 DNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVKEAEAALKSAR 646
Query: 612 KSERKKNQNYFKEIDDLTKEEELT 635
+E++K Q + + + K+ +++
Sbjct: 647 GAEKEKAQKKYDRLKEQLKKLKIS 670
Score = 99.4 bits (246), Expect = 2e-20
Identities = 44/84 (52%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Query: 705 EKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKVF 764
EK ++ ++L++++ + DK++N +ALGTSK+NYIDPR++VA+CKKF+VP+EKVF
Sbjct: 651 EKAQKKYDRLKEQLKKLKISRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVF 710
Query: 765 TKTLRDKFRWAIE---SADENWTF 785
TKT R+KFRWAI+ S+DE + F
Sbjct: 711 TKTHREKFRWAIDMTNSSDEEYVF 734
>CE12282 [L] KOG0981 DNA topoisomerase I
Length = 806
Score = 401 bits (1031), Expect = e-111
Identities = 219/514 (42%), Positives = 304/514 (58%), Gaps = 21/514 (4%)
Query: 137 IELKESSED-FKWWENGDKEDDTIKWTTLKHNGVIFPSTYEPLPAHVKLYYNGKPVDLPP 195
++ +E ED ++WW+ +K+ +KW +L+H G +F Y PLP+HV Y G+ + L
Sbjct: 235 VKKEEDIEDIWEWWKE-EKKPAGVKWNSLQHCGPLFAPPYIPLPSHVHFKYGGEKMKLTL 293
Query: 196 QAEEVAGFFAAILESDHVKIPTFQKNFLHDFLQVLKENGGARNGVQIKEFGKLDFTKIFE 255
+ EE+A F+A +L+ ++ F KNF+ D+ +V+ R I + K DF I
Sbjct: 294 ETEEIAQFYAGVLDHEYSTKEAFNKNFMKDWRKVMTVEERER----IHDLKKCDFRAIDA 349
Query: 256 YFQVQKEQKKQLSPXXXXXXXXXXXXXXXPYKFCEIDGRVEQVGNFRIEPPGLFRGRGAH 315
Y + Q+E +K ++ Y IDG ++V NFRIEPPG+FRGRG H
Sbjct: 350 YQKEQREIRKAMTKEEKLKIKEEKEAEVKIYGIAIIDGHRQKVANFRIEPPGVFRGRGGH 409
Query: 316 PKNGKLKRRVYPEDVVLNLDKEAPIPEPPKGHKWGEIRHDNTVQWLSMWRENISNSFKYV 375
PK G +K+R+ PEDV++N K+ IP+PP GHKW E+RHDNTV WL W E++ KY+
Sbjct: 410 PKMGLIKKRIMPEDVIINCGKDTEIPKPPPGHKWKEVRHDNTVTWLCSWTESVLGQNKYI 469
Query: 376 RFAANSTLKGMSDFKKFEKARMLKDHIDKVRTDYKSKMKSPKTLDRQMAVATYLIDVFAL 435
+S +KG DF+K+E AR LK I +R Y KS + RQ A A Y ID AL
Sbjct: 470 MLNPSSKIKGEKDFEKYETARRLKKKIGGIRERYTDDFKSKEMRVRQRATALYFIDKLAL 529
Query: 436 RAGGEK-ADDEADTVGCCSLRYEHVTL------------RPPNTVVFDFLGKDSIRYYQE 482
RAG EK D+ ADTVGCCSLR EH+ L V FDFLGKDSIRY+
Sbjct: 530 RAGNEKDVDEAADTVGCCSLRVEHIKLFDSAKLNEDDKKEKEFVVEFDFLGKDSIRYFNR 589
Query: 483 VEVDAQVFKNLKIFKRPPSGPGHQLFDRLDPSILNKHLQNYMPGLTAKVFRTYNASKTMQ 542
V V+ +V+KNLKIF P LFDRLD + LN HL++ M GLT KVFRTYNAS T+Q
Sbjct: 590 VSVEKRVYKNLKIFME-GKAPSDDLFDRLDTATLNDHLRSLMDGLTVKVFRTYNASITLQ 648
Query: 543 DQLDLIPN-EGTVAEKILRYNAANRTVAILCNHQRTVTKGHASSVQKATERIEELEWQKV 601
+QL + N + VA KIL YN ANR VAILCNHQR V+KG S+QK ++I++ + +
Sbjct: 649 EQLIKLTNPKDNVAAKILSYNRANRQVAILCNHQRAVSKGFDESMQKLEQKIKDKKKEVK 708
Query: 602 RYKRAILQLDKSERKKNQNYFKEIDDLTKEEELT 635
+ A+ +E++K Q + + + K+ +++
Sbjct: 709 EAEAALKSARGAEKEKAQKKYDRLKEQLKKLKIS 742
Score = 99.4 bits (246), Expect = 2e-20
Identities = 44/84 (52%), Positives = 66/84 (78%), Gaps = 3/84 (3%)
Query: 705 EKLKQNVEKLEKRITNCSLQLKDKEDNSTVALGTSKINYIDPRLSVAFCKKFDVPIEKVF 764
EK ++ ++L++++ + DK++N +ALGTSK+NYIDPR++VA+CKKF+VP+EKVF
Sbjct: 723 EKAQKKYDRLKEQLKKLKISRTDKDENKQIALGTSKLNYIDPRITVAWCKKFEVPLEKVF 782
Query: 765 TKTLRDKFRWAIE---SADENWTF 785
TKT R+KFRWAI+ S+DE + F
Sbjct: 783 TKTHREKFRWAIDMTNSSDEEYVF 806
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.134 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,091,741
Number of Sequences: 60738
Number of extensions: 1851727
Number of successful extensions: 6170
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 6100
Number of HSP's gapped (non-prelim): 22
length of query: 785
length of database: 30,389,216
effective HSP length: 114
effective length of query: 671
effective length of database: 23,465,084
effective search space: 15745071364
effective search space used: 15745071364
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)