ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1535 good K KOG4392 Transcription RNA polymerase, subunit L
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1535 543604 543242 -121
(121 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL005c [K] KOG4392 RNA polymerase subunit L 202 6e-53
7298381 [K] KOG4392 RNA polymerase subunit L 111 1e-25
Hs5453932 [K] KOG4392 RNA polymerase subunit L 110 2e-25
SPAC3A12.07 [K] KOG4392 RNA polymerase subunit L 108 1e-24
HsM14702177 [K] KOG4392 RNA polymerase subunit L 108 2e-24
Hs21704272 [K] KOG4392 RNA polymerase subunit L 108 2e-24
Hs21704274 [K] KOG4392 RNA polymerase subunit L 106 6e-24
At3g52090 [K] KOG4392 RNA polymerase subunit L 100 4e-22
CE18986 [K] KOG4392 RNA polymerase subunit L 94 4e-20
Hs14702175 [K] KOG4392 RNA polymerase subunit L 55 1e-08
SPAC1687.01 [K] KOG3438 DNA-directed RNA polymerase subunit L 51 2e-07
YNL113w [K] KOG3438 DNA-directed RNA polymerase subunit L 51 3e-07
CE00225 [K] KOG3438 DNA-directed RNA polymerase subunit L 51 3e-07
At2g29540 [K] KOG3438 DNA-directed RNA polymerase subunit L 50 4e-07
ECU05g0275 [K] KOG4392 RNA polymerase subunit L 50 7e-07
Hs7705740 [K] KOG3438 DNA-directed RNA polymerase subunit L 49 9e-07
>YOL005c [K] KOG4392 RNA polymerase subunit L
Length = 120
Score = 202 bits (514), Expect = 6e-53
Identities = 96/118 (81%), Positives = 107/118 (90%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAPDRFELFLL EGESKLK++ DTKA +A+++TFEKEDHTLGNLIRAELL D VLFAA
Sbjct: 1 MNAPDRFELFLLGEGESKLKIDPDTKAPNAVVITFEKEDHTLGNLIRAELLNDRKVLFAA 60
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFETEWNLQTLATED 118
YKVEHP FA+FK+RIQT EGYDPKDALKNACNSIINKL L++NFETEWNLQTLA +D
Sbjct: 61 YKVEHPFFARFKLRIQTTEGYDPKDALKNACNSIINKLGALKTNFETEWNLQTLAADD 118
>7298381 [K] KOG4392 RNA polymerase subunit L
Length = 117
Score = 111 bits (278), Expect = 1e-25
Identities = 56/106 (52%), Positives = 71/106 (66%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL EGE K+ E DTK ++A I T KEDHTLGN+IR +LL+D VLFA
Sbjct: 1 MNAPPTFESFLLYEGEKKIIKELDTKVTNAAIFTINKEDHTLGNMIRNQLLKDPNVLFAG 60
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFE 106
YKV HPL KF +RIQT Y P++A NA ++ +L+ + F+
Sbjct: 61 YKVPHPLEHKFVIRIQTTADYSPQEAFMNAITDLLAELSLFEERFK 106
>Hs5453932 [K] KOG4392 RNA polymerase subunit L
Length = 117
Score = 110 bits (276), Expect = 2e-25
Identities = 53/105 (50%), Positives = 72/105 (68%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL EGE K+ + +DTK +A + T KEDHTLGN+I+++LL+D VLFA
Sbjct: 1 MNAPPAFESFLLFEGEKKITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 60
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNF 105
YKV HPL K +R+QT Y P++A NA +I++L+ L+ F
Sbjct: 61 YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 105
>SPAC3A12.07 [K] KOG4392 RNA polymerase subunit L
Length = 123
Score = 108 bits (270), Expect = 1e-24
Identities = 56/121 (46%), Positives = 77/121 (63%), Gaps = 1/121 (0%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MN P+R+EL L G K+ E D+K+ +A +VT EKEDHTL N++ +LL DE VLFA
Sbjct: 1 MNQPERYELIELM-GLPKVTYELDSKSPNAAVVTLEKEDHTLANMLANQLLSDERVLFAG 59
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFETEWNLQTLATEDYG 120
YKV HPL F +R+QTVE PK + +A S+I L +++ NF EW L+ ++ E
Sbjct: 60 YKVPHPLNHNFILRVQTVEDCSPKQVIVDAAKSLITHLEEIKVNFMREWELKMISVEGVE 119
Query: 121 M 121
M
Sbjct: 120 M 120
>HsM14702177 [K] KOG4392 RNA polymerase subunit L
Length = 115
Score = 108 bits (269), Expect = 2e-24
Identities = 54/107 (50%), Positives = 73/107 (67%), Gaps = 1/107 (0%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL EGE K+ + +DTK +A + T KEDHTLGN+I+++LL+D VLFA
Sbjct: 1 MNAPPAFESFLLFEGE-KITINKDTKVPNACLFTMNKEDHTLGNIIKSQLLKDPQVLFAG 59
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFET 107
YKV HPL K +R+QT Y P++A NA +I++L+ L+ F T
Sbjct: 60 YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRT 106
>Hs21704272 [K] KOG4392 RNA polymerase subunit L
Length = 115
Score = 108 bits (269), Expect = 2e-24
Identities = 54/107 (50%), Positives = 73/107 (67%), Gaps = 1/107 (0%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL EGE K+ + +DTK +A + T KEDHTLGN+I+++LL+D VLFA
Sbjct: 1 MNAPPAFESFLLFEGE-KITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 59
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFET 107
YKV HPL K +R+QT Y P++A NA +I++L+ L+ F T
Sbjct: 60 YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERFRT 106
>Hs21704274 [K] KOG4392 RNA polymerase subunit L
Length = 158
Score = 106 bits (264), Expect = 6e-24
Identities = 53/105 (50%), Positives = 72/105 (68%), Gaps = 1/105 (0%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL EGE K+ + +DTK +A + T KEDHTLGN+I+++LL+D VLFA
Sbjct: 1 MNAPPAFESFLLFEGE-KITINKDTKVPNACLFTINKEDHTLGNIIKSQLLKDPQVLFAG 59
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNF 105
YKV HPL K +R+QT Y P++A NA +I++L+ L+ F
Sbjct: 60 YKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLEERF 104
>At3g52090 [K] KOG4392 RNA polymerase subunit L
Length = 116
Score = 100 bits (248), Expect = 4e-22
Identities = 48/108 (44%), Positives = 68/108 (62%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP+R+E F++PEG K+ ++DTK +A T E+EDHT+GN++R +L DE VLFA
Sbjct: 1 MNAPERYERFVVPEGTKKVSYDRDTKIINAASFTVEREDHTIGNIVRMQLHRDENVLFAG 60
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFETE 108
Y++ HPL K +RI T P A A N + +L L++ FE E
Sbjct: 61 YQLPHPLKYKIIVRIHTTSQSSPMQAYNQAINDLDKELDYLKNQFEAE 108
>CE18986 [K] KOG4392 RNA polymerase subunit L
Length = 122
Score = 93.6 bits (231), Expect = 4e-20
Identities = 49/106 (46%), Positives = 66/106 (62%), Gaps = 1/106 (0%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
MNAP FE FLL + + K +E+DTK +A I T KEDHTLGN+++ +LL+D VLFA
Sbjct: 1 MNAPAAFESFLLLD-DKKFYIEKDTKVPNAAIFTIMKEDHTLGNMLKIQLLKDPEVLFAG 59
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFE 106
YK HPL K +RIQT P DAL A ++ +L+ L+ +
Sbjct: 60 YKNPHPLEHKILLRIQTTNNTTPADALTTAITDLVGELSLLEHRID 105
>Hs14702175 [K] KOG4392 RNA polymerase subunit L
Length = 116
Score = 55.5 bits (132), Expect = 1e-08
Identities = 27/48 (56%), Positives = 35/48 (72%), Gaps = 1/48 (2%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRA 48
MNAP FE FLL EGE K+ + +DTK +A + T KEDHTLGN+I++
Sbjct: 1 MNAPPAFESFLLFEGE-KITINKDTKVPNACLFTINKEDHTLGNIIKS 47
>SPAC1687.01 [K] KOG3438 DNA-directed RNA polymerase subunit L
Length = 95
Score = 51.2 bits (121), Expect = 2e-07
Identities = 29/86 (33%), Positives = 42/86 (48%), Gaps = 2/86 (2%)
Query: 33 VTFE--KEDHTLGNLIRAELLEDEGVLFAAYKVEHPLFAKFKMRIQTVEGYDPKDALKNA 90
VTF+ KEDHTLGN +R ++++ V F Y + HP AK RIQT D L+
Sbjct: 5 VTFQIQKEDHTLGNSLRYVIMKNPEVEFCGYSIPHPSEAKMNFRIQTAPSTTAVDVLRKG 64
Query: 91 CNSIINKLAQLQSNFETEWNLQTLAT 116
+ +I+ + F + T T
Sbjct: 65 LDDLIDLCDAVTEKFTEQLPRDTSTT 90
>YNL113w [K] KOG3438 DNA-directed RNA polymerase subunit L
Length = 142
Score = 50.8 bits (120), Expect = 3e-07
Identities = 33/102 (32%), Positives = 51/102 (49%), Gaps = 8/102 (7%)
Query: 4 PDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAAYKV 63
PDR ++ LL + S E T AS I+ +EDHTLGN +R ++++ V F Y +
Sbjct: 42 PDREKIKLLTQATS----EDGTSASFQIV----EEDHTLGNALRYVIMKNPDVEFCGYSI 93
Query: 64 EHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNF 105
HP +RIQT DAL+ +++ ++S F
Sbjct: 94 PHPSENLLNIRIQTYGETTAVDALQKGLKDLMDLCDVVESKF 135
>CE00225 [K] KOG3438 DNA-directed RNA polymerase subunit L
Length = 144
Score = 50.8 bits (120), Expect = 3e-07
Identities = 33/109 (30%), Positives = 54/109 (49%), Gaps = 7/109 (6%)
Query: 1 MNAPDRFELFLLPEGESKLKVEQDTKASSAIIVTFEKEDHTLGNLIRAELLEDEGVLFAA 60
+N P + ++ +L EQD S+ ++ +E EDHT+GN I+ L + V F
Sbjct: 38 LNVPSKKKMEIL----DPKSFEQDP--SNLTLIMYE-EDHTIGNSIKHILSRMDEVEFCG 90
Query: 61 YKVEHPLFAKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFETEW 109
Y V HPL K R+QT +G + + L A S+ + ++ FE +
Sbjct: 91 YNVPHPLEDKILFRVQTKDGINALEVLAKAFESVEQIFSTIRGKFEESY 139
>At2g29540 [K] KOG3438 DNA-directed RNA polymerase subunit L
Length = 122
Score = 50.4 bits (119), Expect = 4e-07
Identities = 25/73 (34%), Positives = 40/73 (54%), Gaps = 1/73 (1%)
Query: 34 TFEKEDHTLGNLIRAELLEDEGVLFAAYKVEHPLFAKFKMRIQTVEGYDPKDALKNACNS 93
T +EDHTL N +R L +D V AAY + HP + +R+QT G ++ K+AC
Sbjct: 15 TLSEEDHTLANAVRFVLNQDPRVTVAAYTIPHPSLEQVNIRVQTT-GDPAREVFKDACQE 73
Query: 94 IINKLAQLQSNFE 106
++ ++S F+
Sbjct: 74 LMQMNRHVRSVFD 86
>ECU05g0275 [K] KOG4392 RNA polymerase subunit L
Length = 96
Score = 49.7 bits (117), Expect = 7e-07
Identities = 26/78 (33%), Positives = 39/78 (49%)
Query: 29 SAIIVTFEKEDHTLGNLIRAELLEDEGVLFAAYKVEHPLFAKFKMRIQTVEGYDPKDALK 88
+ I + E E HTLGNL+ ELLED+ LF+AY+VEHP +R+ +D L
Sbjct: 17 NTIELRIEDETHTLGNLLSEELLEDKRCLFSAYRVEHPADTHVFLRLTADRDCQVRDLLL 76
Query: 89 NACNSIINKLAQLQSNFE 106
+ + L + +
Sbjct: 77 ETLKRVEEETTSLMNQLK 94
>Hs7705740 [K] KOG3438 DNA-directed RNA polymerase subunit L
Length = 133
Score = 49.3 bits (116), Expect = 9e-07
Identities = 28/98 (28%), Positives = 45/98 (45%), Gaps = 3/98 (3%)
Query: 12 LPEGESKLKVEQDTKASS---AIIVTFEKEDHTLGNLIRAELLEDEGVLFAAYKVEHPLF 68
+ EGE K +E A + + +EDHTLGN +R ++++ V F Y HP
Sbjct: 18 MAEGERKTALEMVQAAGTDRHCVTFVLHEEDHTLGNSLRYMIMKNPEVEFCGYTTTHPSE 77
Query: 69 AKFKMRIQTVEGYDPKDALKNACNSIINKLAQLQSNFE 106
+K +RIQT + + N ++N + FE
Sbjct: 78 SKINLRIQTRGTLPAVEPFQRGLNELMNVCQHVLDKFE 115
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.133 0.372
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,738,437
Number of Sequences: 60738
Number of extensions: 239195
Number of successful extensions: 607
Number of sequences better than 1.0e-05: 16
Number of HSP's better than 0.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 590
Number of HSP's gapped (non-prelim): 16
length of query: 121
length of database: 30,389,216
effective HSP length: 97
effective length of query: 24
effective length of database: 24,497,630
effective search space: 587943120
effective search space used: 587943120
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)