ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1559 good L KOG2327 Replication, recombination and repair DNA-binding subunit of a DNA-dependent protein kinase (Ku70 autoantigen)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1559 550683 552458 592
(592 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR284w [L] KOG2327 DNA-binding subunit of a DNA-dependent prote... 546 e-155
SPCC126.02c [L] KOG2327 DNA-binding subunit of a DNA-dependent p... 102 1e-21
At1g16970 [L] KOG2327 DNA-binding subunit of a DNA-dependent pro... 89 2e-17
Hs4503841 [L] KOG2327 DNA-binding subunit of a DNA-dependent pro... 82 3e-15
Hs22056613 [L] KOG2327 DNA-binding subunit of a DNA-dependent pr... 55 2e-07
At1g48050 [L] KOG2326 DNA-binding subunit of a DNA-dependent pro... 52 3e-06
>YMR284w [L] KOG2327 DNA-binding subunit of a DNA-dependent protein kinase
(Ku70 autoantigen)
Length = 602
Score = 546 bits (1406), Expect = e-155
Identities = 275/585 (47%), Positives = 398/585 (68%), Gaps = 17/585 (2%)
Query: 24 DHKKWRRYEAHDGIVFCIQLTPTMYLSNPDLGGKIQLLEILDSLNDLMSQLVVVMPSTAV 83
D +R+++ H+GI+FCI+L+ TM+ + DL K LLEIL+SL++LMSQLV+ P TA+
Sbjct: 19 DETGYRKFDIHEGILFCIELSETMFKESSDLEYKSPLLEILESLDELMSQLVITRPGTAI 78
Query: 84 GCYIYNCSHPNAEDNVYELIPLRDVNYKNMKRVSDLLEDIEQDRILLKDEIPIADPNNP- 142
GCY Y C+ +A++ +YEL PLRD+N MK+++DLLED+ RI L D +
Sbjct: 79 GCYFYYCNREDAKEGIYELFPLRDINATFMKKLNDLLEDLSSGRISLYDYFMFQQTGSEK 138
Query: 143 -IELSPVLIKVRETFLQPIEGQKQLTNKKIFFFTDDDKPAEFLNVDSRSRLRKVVDDLYD 201
+ LS + + +TFL+ I GQKQL+NK++F FTD DKP E ++D R+RLR++ DL+D
Sbjct: 139 QVRLSVLFTFMLDTFLEEIPGQKQLSNKRVFLFTDIDKPQEAQDIDERARLRRLTIDLFD 198
Query: 202 YYINFVTFFIGSEDKPFDDSMYADILKWGSKINEAQSWLSSH-GPSTNPINASYIKSKVK 260
+NF TFFIG DKPFD+ Y+DIL+ GS NE S GPST PI+A YIKS++
Sbjct: 199 NKVNFATFFIGYADKPFDNEFYSDILQLGSHTNENTGLDSEFDGPSTKPIDAKYIKSRIL 258
Query: 261 RTKEIKRIKFRCPLMLDERADLVVSVNGYTIVSHEIPGSKYKLVYANGSVRREAYSHREY 320
R KE+KRI F+CPL+LDE+ + +V V GYT+ +HE G +YKLVY + +R+EAYS R++
Sbjct: 259 RKKEVKRIMFQCPLILDEKTNFIVGVKGYTMYTHEKAGVRYKLVYEHEDIRQEAYSKRKF 318
Query: 321 LDAETGEAVDNSQLSKVYTFGDEIIELTEDENLKIQSGYSEHESFLKLIGFRSTEQCLHY 380
L+ TGE V + KVY +GD I L++ ++ + Y++ ++FLK+IGFRS+ + +HY
Sbjct: 319 LNPITGEDVTGKTV-KVYPYGDLDINLSDSQDQIVMEAYTQKDAFLKIIGFRSSSKSIHY 377
Query: 381 YDNIDVPAFVVPNEEDYAGSIKTLASLYRTMLFKKKSAMVWGKLRPNSPPSMFVLTPSSK 440
++NID +F+VP+E Y GSI+TLASL + + K K A++WGKL+ NS PS++ L+PSS
Sbjct: 378 FNNIDKSSFIVPDEAKYEGSIRTLASLLKILRKKDKIAILWGKLKSNSHPSLYTLSPSSV 437
Query: 441 KDYNQGFYLYKVPFMEEVRKLPDFLNHSALIESD-DYKVMSKVTETLINFFNLKNGYRPS 499
KDYN+GFYLY+VPF++E+RK P L++ E DY M KVT++++ +FNL++GY PS
Sbjct: 438 KDYNEGFYLYRVPFLDEIRKFPSLLSYDDGSEHKLDYDNMKKVTQSIMGYFNLRDGYNPS 497
Query: 500 DFHNPALQKHFKILREYLLQIEVDKSKPEDEG---------DETLQKVKQIYARIASSAK 550
DF NP LQKH+K+L +YLLQIE + E D++L+K+ I +I S K
Sbjct: 498 DFKNPLLQKHYKVLHDYLLQIETTFDENETPNTKKDRMMREDDSLRKLYYIRNKILESEK 557
Query: 551 SDDVKQQRLVKYLKLWNSFYNRLSNLEVDTKPTK---NKKAKLNL 592
S+D QRL KY+K+WN FY + ++ + K K +KK K N+
Sbjct: 558 SEDPIIQRLNKYVKIWNMFYKKFNDDNISIKEEKKPFDKKPKFNI 602
>SPCC126.02c [L] KOG2327 DNA-binding subunit of a DNA-dependent protein kinase
(Ku70 autoantigen)
Length = 607
Score = 102 bits (255), Expect = 1e-21
Identities = 116/516 (22%), Positives = 222/516 (42%), Gaps = 39/516 (7%)
Query: 37 IVFCIQLTPTMYLSNPDLGGKIQLLEILDSLNDLMSQLVVVMPSTAVGCYIYNCSHPNAE 96
I+F I+++P+M L D L L L +Q V+ PS +G +Y ++
Sbjct: 20 ILFVIEVSPSM-LDPVDEFTPSSLQMALICAYQLAAQRVITNPSDIMGVLLYGTE--SST 76
Query: 97 DNVYELIPLRDVNYKNMKRVSDLLEDIEQDRILLKDEIPIADPNNPIELSPVLIKVRETF 156
+ L D++ + +R+ L + E+D K++ + LS VL F
Sbjct: 77 GRFANQMMLLDIDPPDAERIKSL-QSFEKDFQFSKEKFKPCSCQ--VSLSSVLYHCSVIF 133
Query: 157 LQPIEGQKQLTNKKIFFFTDDDKPAEFLNVDSRSRLRKVVDDLYDYYINFVTFFIGSEDK 216
E + K++F TD+D PA + R + + DL D I F+
Sbjct: 134 TTKAENFE----KRLFLITDNDHPA--WDATERDIILQRAKDLRDLDIQVHPVFLDPPTH 187
Query: 217 PFDDSMYADILKWGSKINEAQSWLSSHGPSTNPINASYIKSKVKRTKEIKRIKFRCPLML 276
F +++ + + S L + G + ++ + + ++ KR F L +
Sbjct: 188 SFRINIFYSDFLYIVYGRQDVSNLVNRGQA----QLQHMLNMITALQKPKRAHFH--LKM 241
Query: 277 DERADLVVSVNGYTIVSHEIPGSKYKLVYANGSVRREAYSHREYLDAETGEAVDNSQLSK 336
D D+ + V + ++ + +K VYA G A + + T + + ++ +
Sbjct: 242 DLGNDVRIGVEAFILLKR-LESAKTNWVYAKGERFAVAVPQSKQVSFATKKELKKDEIRR 300
Query: 337 VYTFGDEIIELTEDENLKIQSGYSEHESFLKLIGFRSTEQCLHYYDNIDVPAFVVPNEED 396
Y++G + DE K++S L++IGFR L + + F+ P +++
Sbjct: 301 SYSYGGSSVVFGSDELNKVRSF---EPPTLRIIGFRDFST-LKPWHCLKPAVFLRPKDDE 356
Query: 397 YAGSIKTLASLYRTMLFKKKSAMVWGKLRPNSPPSMFVL--TPSS---KKDYNQ--GFYL 449
GS +++++ +L K + W RPN+ P + TP S + D+ G +L
Sbjct: 357 IIGSGAVFSAIHKKLLASNKIGIAWFVSRPNANPCFVAMLATPGSIHIRDDFELPLGIFL 416
Query: 450 YKVPFMEEVRKLPDFLNHSALIESDDYKVMSKVTETLINFFNLKNGYRPSDFHNPALQKH 509
++P +++R LP + + S+ + M ++ + L++ Y+P ++NP+LQ H
Sbjct: 417 VQLPTADDIRSLPPINPNPISMPSNLIETMQRILRGM----ELRS-YQPGKYNNPSLQWH 471
Query: 510 FKILREYLLQIEVDKSKPEDEGDETLQKVKQIYARI 545
+K+L+ L E+ P D D TL K K I R+
Sbjct: 472 YKVLQALALDEEI----PTDFVDNTLPKYKAIQKRV 503
>At1g16970 [L] KOG2327 DNA-binding subunit of a DNA-dependent protein kinase
(Ku70 autoantigen)
Length = 610
Score = 88.6 bits (218), Expect = 2e-17
Identities = 89/384 (23%), Positives = 176/384 (45%), Gaps = 42/384 (10%)
Query: 161 EGQKQLTNKKIFFFTDDDKPAEFLNVDSRSRLRKVV----DDLYDYYINFVTFFIGSEDK 216
+G + +K++F FT++D P + + + + + D D I+ + DK
Sbjct: 161 KGSLKTADKRMFLFTNEDDPFGSMRISVKEDMTRTTLQRAKDAQDLGISIELLPLSQPDK 220
Query: 217 PFDDSM-YADILKWGSKINEAQSWLSSHGPSTNPINASYIKSKVKRTKEIKRIKFRCPLM 275
F+ ++ Y D++ G +E ++ S G + K ++K+ KRI R +
Sbjct: 221 QFNITLFYKDLI--GLNSDELTEFMPSVGQKLEDM-----KDQLKKRVLAKRIAKRITFV 273
Query: 276 LDERADLVVSVNGYTIVSHEIPGSKYKLVYANGSVRREAYSHREYLDAETGEAVDNSQLS 335
+ + L + +NGY ++ IPGS + + + + R Y+ +TG A+ +
Sbjct: 274 ICD--GLSIELNGYALLRPAIPGS---ITWLDSTTNLPVKVERSYICTDTG-AIMQDPIQ 327
Query: 336 KVYTFGDEIIELTEDENLKIQSGYSEHESFLKLIGFRSTEQCLHYYDNIDVPAFVVPNEE 395
++ + ++ I T +E +++ + H L+L+GF+ CL Y N+ F+ P+++
Sbjct: 328 RIQPYKNQNIMFTVEELSQVKRISTGH---LRLLGFKPLS-CLKDYHNLKPSTFLYPSDK 383
Query: 396 DYAGSIKTLASLYRTMLFKKKSAMVWGKLRPNSPPSMFVLTPSSKKDYNQGFYLYKVPFM 455
+ GS + +L+R+M+ ++ A+ + +PP + L K L+ P
Sbjct: 384 EVIGSTRAFIALHRSMIQLERFAVAF--YGGTTPPRLVALVAQLLK------CLFLKPKQ 435
Query: 456 EEVR----KLPDFLNHSALIESDDYKVMSKVTETLINFFNLKNGYRPSDFHNPALQKHFK 511
+E+ +L +A SDD K L+ LK+ + F NPALQ+H+
Sbjct: 436 DEIESDGGQLHSKPGVAAPRASDD---QLKKASALMRRLELKD-FSVCQFANPALQRHYA 491
Query: 512 ILREYLLQIEVDKSKPEDEGDETL 535
IL+ I +D+++ + DETL
Sbjct: 492 ILQ----AIALDENELRETRDETL 511
>Hs4503841 [L] KOG2327 DNA-binding subunit of a DNA-dependent protein kinase
(Ku70 autoantigen)
Length = 609
Score = 81.6 bits (200), Expect = 3e-15
Identities = 125/532 (23%), Positives = 221/532 (41%), Gaps = 56/532 (10%)
Query: 30 RYEAHDGIVFCIQLTPTMYLSNPDLGGKIQLLEI-LDSLNDLMSQLVVVMPSTAVGCYIY 88
+Y D ++F + + M+ S + ++ ++ + + + ++ + Y
Sbjct: 31 KYSGRDSLIFLVDASKAMFESQSE--DELTPFDMSIQCIQSVYISKIISSDRDLLAVVFY 88
Query: 89 NCSHPNAEDNVYELIPLRDVNYKNMKRVSDLLE-DIEQDRILLKDEIPIADPNNPIELSP 147
N + L++++ KR+ +L + +Q + +D + + LS
Sbjct: 89 GTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQD---MMGHGSDYSLSE 145
Query: 148 VLIKVRETFLQPIEGQKQLTNKKIFFFTDDDKPAEFLNVDSRSRLRKVVDDLYDYYINFV 207
VL F + Q ++++K+I FT++D P + + SR R DL D I F+
Sbjct: 146 VLWVCANLFS---DVQFKMSHKRIMLFTNEDNPHGNDSAKA-SRARTKAGDLRDTGI-FL 200
Query: 208 TFFIGSEDKPFDDSM-YADILKWGSKINEAQSWLSSHGPSTNPINASYIKSKVKRTKEIK 266
+ FD S+ Y DI+ I E + L H ++ + K + K T+ K
Sbjct: 201 DLMHLKKPGGFDISLFYRDIIS----IAEDED-LRVHFEESSKLEDLLRKVRAKETR--K 253
Query: 267 RIKFRCPLMLDERADLVVSVNGYTIVSHEIPGSKYKLVY-ANGSVRREAYSHREYLDAET 325
R R L L++ D+V+SV Y +V + KL N V+ + + + T
Sbjct: 254 RALSRLKLKLNK--DIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRT----FNTST 307
Query: 326 GEAV--DNSQLSKVYTFGDEIIELTEDENLKIQSGYSEHESFLKLIGFRSTEQCL-HYYD 382
G + +++ S++Y I+E E E LK + L L+GF+ H+Y
Sbjct: 308 GGLLLPSDTKRSQIYGSRQIILEKEETEELK-----RFDDPGLMLMGFKPLVLLKKHHY- 361
Query: 383 NIDVPAFVVPNEEDYAGSIKTLASLYRTMLFKKKSAMVWGKLRPNSPPSMFVLTPSSKKD 442
+ FV P E GS ++L L K+ +A+ R N PP L P ++
Sbjct: 362 -LRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEEL 420
Query: 443 YNQ-------GFYLYKVPFMEEVRKLPDFLNHSALIESDDYKVMSKVTETLINFFNLKNG 495
+Q GF L +PF ++ RK+P + + M + E L+
Sbjct: 421 DDQKIQVTPPGFQLVFLPFADDKRKMP--FTEKIMATPEQVGKMKAIVE------KLRFT 472
Query: 496 YRPSDFHNPALQKHFKILREYLLQIEVDKSKPEDEGDETLQKVKQIYARIAS 547
YR F NP LQ+HF+ L + +D +PE D TL KV+ + R+ S
Sbjct: 473 YRSDSFENPVLQQHFRNLE----ALALDLMEPEQAVDLTLPKVEAMNKRLGS 520
>Hs22056613 [L] KOG2327 DNA-binding subunit of a DNA-dependent protein kinase
(Ku70 autoantigen)
Length = 479
Score = 55.5 bits (132), Expect = 2e-07
Identities = 76/335 (22%), Positives = 134/335 (39%), Gaps = 37/335 (11%)
Query: 223 YADILKWGSKINEAQSWLSSHGPSTNPINASYIKSKVKRTKE--IKRIKFRCPLMLDERA 280
+ D++ GS + ++ H ++ + K + K T++ + R+K +
Sbjct: 129 FEDLMGHGSDYSFSEVLNPVHFEESSNLEDLLQKVRAKETRKWALSRLKLKL------NK 182
Query: 281 DLVVSVNGYTIVSHEIPGSKYKLVYANGSVRREAYSHREYLDAETGEAVDNSQLSKVYTF 340
D+V+SV Y++V + KL Y + + S + ++ + +++ S++Y
Sbjct: 183 DIVISVGIYSLVQKALKPPSIKL-YRKTNEPVKTKSRKFNINTGSLLLPSDTKRSQIYGS 241
Query: 341 GDEIIELTEDENLKIQSGYSEHESFLKLIGFRSTEQCL-HYYDNIDVPAFVVPNEEDYAG 399
I+E E E LK E L L+ F+ H+Y + F P E G
Sbjct: 242 HQIILEKEETEELKWFD-----EPALMLMVFKPLVMLKEHHYPRPSL--FTYPEESPVNG 294
Query: 400 SIKTLASLYRTMLFKKKSAMVWGKLRPNSPPSMFVLTPSSKKDYNQ-------GFYLYKV 452
S ++L L K A+ R N PP + L ++ +Q GF L +
Sbjct: 295 SSTLFSALLIKCLEKVVIALCRYTPRRNIPPYLVALVSQEEELDDQKIQVTTPGFQLVFL 354
Query: 453 PFMEEVRKLPDFLNHSALIESDDYKVMSKVTETLINFFNLKNGYRPSDFHNPALQKHFKI 512
PF ++ RK+P + + M + + L YR F NP LQ+HF
Sbjct: 355 PFADDKRKVP--FTEKVMATPEQVDNMKAIIQKLCFT------YRSDSFENPVLQQHFGN 406
Query: 513 LREYLLQIEVDKSKPEDEGDETLQKVKQIYARIAS 547
L +L + E D L KV+++ R+ S
Sbjct: 407 LEPLILYL-----MEEQAVDLILPKVEEMNKRLGS 436
>At1g48050 [L] KOG2326 DNA-binding subunit of a DNA-dependent protein kinase
(Ku80 autoantigen)
Length = 696
Score = 51.6 bits (122), Expect = 3e-06
Identities = 59/241 (24%), Positives = 108/241 (44%), Gaps = 25/241 (10%)
Query: 323 AETGEAVDNSQLSKVYTFGDEIIELTED--ENLKIQSGYSEHESFLKLIGFRSTEQCLHY 380
AE+ E + + K + +G ++I ++ D E LK ++ + +KL+GF L +
Sbjct: 291 AESTEVIAPEERIKGFRYGPQVIPISPDQIETLKFKT-----DKGMKLLGFTEASNILRH 345
Query: 381 YDNIDVPAFVV-PNEEDYAGSIKTLASLYRTMLFKKKSAMV---WGKLRPNSPPSMFVLT 436
Y DV V P++E S+ ++++ R M K A+V W + N +
Sbjct: 346 YYMKDVNIVVPDPSKEK---SVLAVSAIAREMKETNKVAIVRCVWRNGQGNVVVGVLTPN 402
Query: 437 PSSKKDYNQGFYLYKVPFMEEVRKLPDFLNHSALIES-DDYKVMSKVTETLINFFNL--- 492
S + D FY +PF E+VR+ P F + + L S + V + L+ +L
Sbjct: 403 VSERDDTPDSFYFNVLPFAEDVREFP-FPSFNKLPSSWKPDEQQQAVADNLVKMLDLAPS 461
Query: 493 --KNGYRPSDFHNPALQKHFKILREYLLQIEVDKSKPEDEGDETLQKVKQIYARIASSAK 550
+ +P NP LQ+ ++ L L D + P +G T +++ + ++S+ K
Sbjct: 462 AEEEVLKPDLTPNPVLQRFYEYLE--LKSKSTDATLPPMDG--TFKRLMEQDPELSSNNK 517
Query: 551 S 551
S
Sbjct: 518 S 518
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.390
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,629,605
Number of Sequences: 60738
Number of extensions: 1716132
Number of successful extensions: 4913
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 4895
Number of HSP's gapped (non-prelim): 7
length of query: 592
length of database: 30,389,216
effective HSP length: 112
effective length of query: 480
effective length of database: 23,586,560
effective search space: 11321548800
effective search space used: 11321548800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)