ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1597 good J KOG2255 Translation, ribosomal structure and biogenesis Peptidyl-tRNA hydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1597 564762 565319 186
(186 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR189w [J] KOG2255 Peptidyl-tRNA hydrolase 120 8e-28
SPBC2D10.15c [J] KOG2255 Peptidyl-tRNA hydrolase 82 3e-16
At1g18440 [J] KOG2255 Peptidyl-tRNA hydrolase 66 2e-11
At5g19830 [J] KOG2255 Peptidyl-tRNA hydrolase 56 2e-08
Hs17449452 [J] KOG2255 Peptidyl-tRNA hydrolase 54 1e-07
>YHR189w [J] KOG2255 Peptidyl-tRNA hydrolase
Length = 190
Score = 120 bits (302), Expect = 8e-28
Identities = 73/175 (41%), Positives = 95/175 (53%), Gaps = 9/175 (5%)
Query: 1 MSAQKLLCLSALGNPEPLYRSTRHNAGVIMLRMLKNEFCPDA--VLKTSNVSPHIQYCHE 58
MS + L L+ +GNPEP Y TRHN G+ ML +L+ N + Y +
Sbjct: 1 MSGKWRLVLTGIGNPEPQYAGTRHNVGLYMLELLRKRLGLQGRTYSPVPNTGGKVHYIED 60
Query: 59 PRLNLLMLFNKSQFMNLSGHAMIPVW-RKLPHDALHVVLHDELNIPIGNVQLRKPGTSFR 117
+L + Q+MNLSG + VW R + A HVV+HDEL++ G VQLR P TS R
Sbjct: 61 EHCTILR--SDGQYMNLSGEQVCKVWARYAKYQARHVVIHDELSVACGKVQLRAPSTSIR 118
Query: 118 GHNGLRDMIKMKGDD-KFHKLGIGIDR---PNSRDPRIVADYVLANFTKGEMETL 168
GHNGLR ++K G F KL IGI R SRDP V+ +VL T E++TL
Sbjct: 119 GHNGLRSLLKCSGGRVPFAKLAIGIGREPGSRSRDPASVSRWVLGALTPQELQTL 173
>SPBC2D10.15c [J] KOG2255 Peptidyl-tRNA hydrolase
Length = 218
Score = 82.4 bits (202), Expect = 3e-16
Identities = 60/190 (31%), Positives = 96/190 (49%), Gaps = 31/190 (16%)
Query: 12 LGNPEPLYRSTRHNAGVIMLRMLKNEFCPDAVLKTSNVSPHIQYCHEPRLNLLMLFNKSQ 71
LGNP + +RH+ G +M+ M + S P + L L++ +
Sbjct: 36 LGNPGSAFVKSRHSLGKLMVSMYAD----------SMAFPKNWPSTKENLTLVL---STS 82
Query: 72 FMNLSGHAMIPVW----RKL-PHDAL-HVVLHDELNIPIGNVQLRKPGTSFRGHNGLRDM 125
+MN SG + + RK+ P D + +VV+HDEL + +G V+LR PG S RGHNG+R
Sbjct: 83 YMNDSGKQLKKISNDFVRKVSPLDKIVYVVVHDELELDLGKVKLRLPGGSHRGHNGIRSC 142
Query: 126 IKMKGDD------------KFHKLGIGIDRPNSRDPRIVADYVLANFTKGEMETLQYDSF 173
+ G + F+++G+GI R SR+ V+DYVL+ F EM+ ++ D F
Sbjct: 143 QEFLGKEVRYTFNFITNSKSFYRIGLGIGRCESRNREDVSDYVLSKFNSNEMKLIETDIF 202
Query: 174 QKAVEHVRKL 183
K +++L
Sbjct: 203 HKFCNILQQL 212
>At1g18440 [J] KOG2255 Peptidyl-tRNA hydrolase
Length = 298
Score = 66.2 bits (160), Expect = 2e-11
Identities = 60/187 (32%), Positives = 95/187 (50%), Gaps = 23/187 (12%)
Query: 12 LGNPEPLYRSTRHNAGVIMLRMLKNEFCPDAVLKTSNVSPHIQYCHEPRLNL-LMLFNKS 70
LGNP Y+ TRHN G M+ L + + + V+ + N+ +ML
Sbjct: 100 LGNPGKKYQGTRHNVGFEMVDALADA----EGISMNTVNFKALFGKGVIGNIPIMLAKPQ 155
Query: 71 QFMNLSGHAM--IPVWRKLPHDALHVVLHDELNIPIGNVQLRKPGTSFRGHNG------- 121
FMNLSG ++ I + K+P + +V++D+L++P G ++L G GHNG
Sbjct: 156 TFMNLSGESVGQIVSFYKIPLKQV-LVVYDDLDLPFGKLRLLPKG-GHGGHNGYNAFHSL 213
Query: 122 ---LRDMI-KMKGDDKFHKLGIGIDRPNSRDPRIVADYVLANFTKGEMETLQYDSFQKAV 177
+R +I ++KG F +L IGI RP + A++VL F + E E L + +FQ +
Sbjct: 214 SGLMRSIIDRLKGSRDFPRLRIGIGRPPGK--MDTANFVLRQFNRQEQEELDH-TFQTGL 270
Query: 178 EHVRKLL 184
E +R LL
Sbjct: 271 EAIRILL 277
>At5g19830 [J] KOG2255 Peptidyl-tRNA hydrolase
Length = 234
Score = 56.2 bits (134), Expect = 2e-08
Identities = 45/144 (31%), Positives = 79/144 (54%), Gaps = 11/144 (7%)
Query: 12 LGNPEPLYRSTRHNAGVIMLRMLKNEFCPDAVLKTSNVSPHIQYCHEPRLNLLMLFNKSQ 71
LGNP Y+ TRHN G M+ + F ++ + V+ +L ++ K Q
Sbjct: 24 LGNPGDKYKGTRHNIGFEMI----DVFAESVGIQMNLVNFKAIMGQGFVADLPVILAKPQ 79
Query: 72 -FMNLSGHAMIPV--WRKLPHDALHVVLHDELNIPIGNVQLRKPGTSFRGHNGLRDMIK- 127
+MNLSG + P+ + KLP + + +V+HD++ +P G ++L++ G HNGL+ ++
Sbjct: 80 TYMNLSGESSGPLAAYYKLPLNRV-LVVHDDMQLPCGVLRLQEKG-GHGCHNGLKSVMNH 137
Query: 128 MKGDDKFHKLGIGIDR-PNSRDPR 150
+G+ +F +L IGI + P DP+
Sbjct: 138 FRGNREFARLRIGIGKPPGQMDPK 161
>Hs17449452 [J] KOG2255 Peptidyl-tRNA hydrolase
Length = 227
Score = 53.9 bits (128), Expect = 1e-07
Identities = 48/162 (29%), Positives = 74/162 (45%), Gaps = 8/162 (4%)
Query: 9 LSALGNPEPLYRSTRHNAGVIMLRMLKNEF-CPDAVLKTSNVSPHIQYCHEPRLNLLMLF 67
++ LGNP TRH+ G+ +L L ++ + + + + L++L
Sbjct: 46 VAGLGNPG--LPGTRHSVGMAVLGQLARRLGVAESWTRDRHCAADLALAPLGDAQLVLL- 102
Query: 68 NKSQFMNLSGHAMIPVWRKLPHDALHVVL-HDELNIPIGNVQLRKPGTSFRGHNGLRDMI 126
+ MN +G ++ A V L HDEL+ P+G + L K G S RGHNG+R I
Sbjct: 103 RPRRLMNANGRSVARAAELFGLTAEEVYLVHDELDKPLGRLAL-KLGGSARGHNGVRSCI 161
Query: 127 KMKGDDKFHKLGIGIDRPNSRDPRIVADYVLANFTKGEMETL 168
+ +L +GI RP P V +VL F+ E E L
Sbjct: 162 SCLNSNAMPRLRVGIGRP--AHPEAVQAHVLGCFSPAEQELL 201
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.139 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,600,636
Number of Sequences: 60738
Number of extensions: 483703
Number of successful extensions: 836
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 826
Number of HSP's gapped (non-prelim): 5
length of query: 186
length of database: 30,389,216
effective HSP length: 100
effective length of query: 86
effective length of database: 24,315,416
effective search space: 2091125776
effective search space used: 2091125776
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)