ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1601 good O KOG3273 Posttranslational modification, protein turnover, chaperones Predicted RNA-binding protein Pno1p interacting with Nob1p and involved in 26S proteasome assembly
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1601 567195 566374 -274
(274 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR145c [O] KOG3273 Predicted RNA-binding protein Pno1p interact... 388 e-108
SPAC2C4.11c [O] KOG3273 Predicted RNA-binding protein Pno1p inte... 298 6e-81
7295605 [O] KOG3273 Predicted RNA-binding protein Pno1p interact... 248 5e-66
Hs10047140 [O] KOG3273 Predicted RNA-binding protein Pno1p inter... 243 2e-64
At3g13230 [O] KOG3273 Predicted RNA-binding protein Pno1p intera... 234 1e-61
CE14896 [O] KOG3273 Predicted RNA-binding protein Pno1p interact... 220 1e-57
ECU11g0750 [O] KOG3273 Predicted RNA-binding protein Pno1p inter... 182 3e-46
ECU04g0510 [JD] KOG2874 rRNA processing protein 50 3e-06
>YOR145c [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 274
Score = 388 bits (996), Expect = e-108
Identities = 201/274 (73%), Positives = 221/274 (80%)
Query: 1 MVAPTALKKSDVSEGTVPVTTTSRIVGVDNTEKIXXXXXXXXXXXXXXXXAMLVDXXXXX 60
MVAPTALKK+ V+ + +SRI+G++NTE I +
Sbjct: 1 MVAPTALKKATVTPVSGQDGGSSRIIGINNTESIDEDDDDDVLLDDSDNNTAKEEVEGEE 60
Query: 61 XXXXXXXDAGVVLDAEGKPRFTSAASSAQTKVKLESRKVSVPPHRMTPLKNNWSKIYPPL 120
VV+D +GKPRFTSA+ + K+K ESRK+ VPPHRMTPL+N+W+KIYPPL
Sbjct: 61 GSRKTHESKTVVVDDQGKPRFTSASKTQGNKIKFESRKIMVPPHRMTPLRNSWTKIYPPL 120
Query: 121 VDHLKLQVRMNLKTKSVELRTHPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDL 180
V+HLKLQVRMNLKTKSVELRT+PK TTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDL
Sbjct: 121 VEHLKLQVRMNLKTKSVELRTNPKFTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDL 180
Query: 181 YIETFEIKDVKTLQGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHI 240
YIETFE+KDVKTL GDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHI
Sbjct: 181 YIETFEVKDVKTLTGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHI 240
Query: 241 RMARESVVSLILGSPPGKVYGNLRTVASRLKERY 274
RMARESVVSLILGSPPGKVYGNLRTVASRLKERY
Sbjct: 241 RMARESVVSLILGSPPGKVYGNLRTVASRLKERY 274
>SPAC2C4.11c [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 258
Score = 298 bits (763), Expect = 6e-81
Identities = 150/203 (73%), Positives = 175/203 (85%), Gaps = 3/203 (1%)
Query: 73 LDAEGKPRFTSAASSAQTK--VKLESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRM 130
+D EG P+F A +SA+ K K + R+V +PPHRMTPL+N W K+YPPLV+HL LQVRM
Sbjct: 56 MDIEGAPKFAPAKTSAEKKRGAKPQMRRVPIPPHRMTPLRNVWPKLYPPLVEHLLLQVRM 115
Query: 131 NLKTKSVELRTHPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDV 190
N K++SVELR K T DPGALQKG DF++AF LGFD+DD+IALLRLDDLYI+TFEIKDV
Sbjct: 116 NTKSRSVELR-ESKATKDPGALQKGMDFVQAFALGFDIDDAIALLRLDDLYIDTFEIKDV 174
Query: 191 KTLQGDHLSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSL 250
KTLQGDHLSRAIGRIAG+ GKTKFAIENA+RTRIVLADSKIHILGGFT+IR+A+++VVSL
Sbjct: 175 KTLQGDHLSRAIGRIAGQGGKTKFAIENASRTRIVLADSKIHILGGFTNIRIAKDAVVSL 234
Query: 251 ILGSPPGKVYGNLRTVASRLKER 273
ILGSPPGKVY NLR A+R KER
Sbjct: 235 ILGSPPGKVYANLRNAAARAKER 257
>7295605 [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 240
Score = 248 bits (634), Expect = 5e-66
Identities = 124/194 (63%), Positives = 157/194 (80%), Gaps = 4/194 (2%)
Query: 83 SAASSAQTKVKL---ESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSVEL 139
S+ +SA K K E RKVSVPPHR + LK +W KI+ P+V+H+KLQ+R N+K + VEL
Sbjct: 47 SSRASAPPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVEL 106
Query: 140 RTHPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVKTLQGDHLS 199
R P+ T D LQ+GADF++AF GF++DD++ALLRL+DL++E+FEIKDVKTL+GDH S
Sbjct: 107 RVGPE-TPDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQS 165
Query: 200 RAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKV 259
RAIGR+AGK G+TKF IEN T+TRIVLADSKIHILG + +I++AR +V +LILGSPP KV
Sbjct: 166 RAIGRLAGKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKV 225
Query: 260 YGNLRTVASRLKER 273
YGNLR VASRL ER
Sbjct: 226 YGNLRAVASRLSER 239
>Hs10047140 [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 252
Score = 243 bits (621), Expect = 2e-64
Identities = 120/197 (60%), Positives = 152/197 (76%), Gaps = 1/197 (0%)
Query: 78 KPRFTSAASSAQTKVKLESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSV 137
+P F K E+RK+ VP +R TPLK NW KI+ P+V+HL LQ+R NLK+++V
Sbjct: 57 RPVFPPLCGDGLLSGKEETRKIPVPANRYTPLKENWMKIFTPIVEHLGLQIRFNLKSRNV 116
Query: 138 ELRTHPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVKTLQGDH 197
E+RT K T D AL K ADF+KAF LGF ++D++AL+RLDDL++E+FEI DVK L+GDH
Sbjct: 117 EIRTC-KETKDVSALTKAADFVKAFILGFQVEDALALIRLDDLFLESFEITDVKPLKGDH 175
Query: 198 LSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPG 257
LSRAIGRIAGK GKTKF IEN TRTRIVLAD K+HILG F +I+MAR ++ +LILG+PP
Sbjct: 176 LSRAIGRIAGKGGKTKFTIENVTRTRIVLADVKVHILGSFQNIKMARTAICNLILGNPPS 235
Query: 258 KVYGNLRTVASRLKERY 274
KVYGN+R VASR +R+
Sbjct: 236 KVYGNIRAVASRSADRF 252
>At3g13230 [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 215
Score = 234 bits (596), Expect = 1e-61
Identities = 113/195 (57%), Positives = 153/195 (77%), Gaps = 1/195 (0%)
Query: 78 KPRFTSAASSAQTKVKLESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSV 137
KP F + + K++ RK++VPP+R +PLK W IY P+ D +K+ +RMNLK + V
Sbjct: 22 KPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLDIYTPIYDQMKVDIRMNLKARKV 81
Query: 138 ELRTHPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVKTLQGDH 197
EL+T T D LQK ADF+ AF LGFD+ D+I+LLR+D+LY+E+FEIKDVKTL+G+H
Sbjct: 82 ELKTRAD-TPDISNLQKSADFVHAFMLGFDIPDAISLLRMDELYVESFEIKDVKTLKGEH 140
Query: 198 LSRAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPG 257
LSRAIGR++GK GKTKFAIEN+T+TRIV+AD++IHILG F++I++AR S+ SLI+GSP G
Sbjct: 141 LSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGAFSNIKVARSSLCSLIMGSPAG 200
Query: 258 KVYGNLRTVASRLKE 272
KVY LR+V++RL E
Sbjct: 201 KVYSKLRSVSARLNE 215
>CE14896 [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 277
Score = 220 bits (561), Expect = 1e-57
Identities = 116/194 (59%), Positives = 150/194 (76%), Gaps = 3/194 (1%)
Query: 82 TSAASSAQTK-VKLESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSVELR 140
T SS +TK VK ESR V VP HR TPLK+NW I+ P+V +L LQVR NLK + VE+R
Sbjct: 84 TGPKSSKRTKGVKGESRVVPVPKHRYTPLKDNWVNIFTPIVKNLGLQVRFNLKKRQVEIR 143
Query: 141 THPKHTTDPGALQKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVK-TLQGDHLS 199
+P D LQK DF++AF LGF+++D+IAL+RLD L++ETFE+ DVK +L+GDH+S
Sbjct: 144 -NPVDREDTTDLQKATDFVRAFILGFEVNDAIALIRLDHLFLETFEVADVKHSLKGDHVS 202
Query: 200 RAIGRIAGKDGKTKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKV 259
RAIGRIAGKDG+TK IEN T+TRIV+A++KIHILG + ++++AR +V SLILGS P KV
Sbjct: 203 RAIGRIAGKDGRTKLVIENTTKTRIVVANTKIHILGAYQNLKLARNAVCSLILGSNPSKV 262
Query: 260 YGNLRTVASRLKER 273
YG+LR +ASR ER
Sbjct: 263 YGSLRNMASRGAER 276
>ECU11g0750 [O] KOG3273 Predicted RNA-binding protein Pno1p interacting with
Nob1p and involved in 26S proteasome assembly
Length = 196
Score = 182 bits (463), Expect = 3e-46
Identities = 82/181 (45%), Positives = 133/181 (73%), Gaps = 1/181 (0%)
Query: 94 LESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSVELRTHPKHTTDPGALQ 153
+++R V + P +M +K+ W KIY P+V+ K+Q+RMN+K KSV++RT +HT DP L+
Sbjct: 4 IQTRSVPIHPRKMKSMKDEWMKIYTPIVEMCKVQIRMNIKGKSVDMRTC-EHTEDPSYLE 62
Query: 154 KGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVKTLQGDHLSRAIGRIAGKDGKTK 213
+ A +I+A +GF ++D+IALL+ D++++ FEI +VKTL+G H+ RA+GRI G++GKT+
Sbjct: 63 RSAQYIEAINIGFPIEDAIALLKFSDVFLDRFEISEVKTLRGLHIERAVGRIIGREGKTR 122
Query: 214 FAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLKER 273
AIE +R++I++ IH+LG + R+AR+++ LI+GS PG ++ LR + SRLKE+
Sbjct: 123 DAIEEFSRSKIIVQGQTIHLLGTVENTRIARDAICRLIMGSQPGSIFNRLRIINSRLKEK 182
Query: 274 Y 274
Y
Sbjct: 183 Y 183
>ECU04g0510 [JD] KOG2874 rRNA processing protein
Length = 283
Score = 50.1 bits (118), Expect = 3e-06
Identities = 44/181 (24%), Positives = 86/181 (47%), Gaps = 8/181 (4%)
Query: 93 KLESRKVSVPPHRMTPLKNNWSKIYPPLVDHLKLQVRMNLKTKSVELRTHPKHTTDPGAL 152
++ S V P +R ++ I L + K+ R++L+ + +++ T+ + T DP
Sbjct: 18 EMSSEAVMFPKYREKYIEQTQKYIVRAL-EAKKISCRIDLEKRVIDVMTN-RSTRDPFIF 75
Query: 153 QKGADFIKAFTLGFDLDDSIALLRLDDLYIETFEIKDVKTLQGDHL-SRAIGRIAGKDGK 211
K +F+K G +++++ +L D+ + E +IK K + D + + R+ G
Sbjct: 76 IKAVNFVKLVGRGVGIEEAMKVLE-DEYFCEVIDIK--KMVSSDKVFEKRRDRLVGPKEM 132
Query: 212 TKFAIENATRTRIVLADSKIHILGGFTHIRMARESVVSLILGSPPGKVYGNLRTVASRLK 271
T AI+ TR +++ + I+GGF + A++ VV + P +Y R V R
Sbjct: 133 TLKAIQILTRCYVLVHGKTVSIIGGFRGVEEAKKIVVDCMNNIHP--MYQIKRLVEKRKM 190
Query: 272 E 272
E
Sbjct: 191 E 191
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,625,358
Number of Sequences: 60738
Number of extensions: 485210
Number of successful extensions: 1175
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1159
Number of HSP's gapped (non-prelim): 8
length of query: 274
length of database: 30,389,216
effective HSP length: 105
effective length of query: 169
effective length of database: 24,011,726
effective search space: 4057981694
effective search space used: 4057981694
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)