ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1622 good G KOG4123 Carbohydrate transport and metabolism Putative alpha 1,2 mannosyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1622 575187 573598 -530
(530 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR149c [G] KOG4123 Putative alpha 12 mannosyltransferase 338 1e-92
SPAC4G8.12c [G] KOG4123 Putative alpha 12 mannosyltransferase 151 3e-36
Hs13376765 [G] KOG4123 Putative alpha 12 mannosyltransferase 98 4e-20
7291781_1 [G] KOG4123 Putative alpha 12 mannosyltransferase 71 5e-12
Hs20127468 [MO] KOG1771 GPI-alpha-mannosyltransferase III (GPI10... 52 2e-06
>YOR149c [G] KOG4123 Putative alpha 12 mannosyltransferase
Length = 516
Score = 338 bits (867), Expect = 1e-92
Identities = 194/499 (38%), Positives = 279/499 (55%), Gaps = 19/499 (3%)
Query: 46 PSYIHPDEHFQTLEPIFXXXXXXXXXXXXEFLPENPCRSITILRLYYTPLLWLNQHIFHX 105
PSYIHPDEHFQ +E + EF + RS L L Y PL + + F
Sbjct: 22 PSYIHPDEHFQCIEILAMQFMKVKGTIPWEFKSKFAARSYGPLLLVYGPLFTILES-FPE 80
Query: 106 XXXXXXXXXXXXXXXXXTAVVIFFLEFCEVSITHKTKA-----KFFIRTSYVTWVFQSHT 160
++ + I + KA K + TSYVTW +Q+HT
Sbjct: 81 IQDNPALILYSMRLQNYVMYLLCYHFLIPKLIRDERKAVQFMQKSLLLTSYVTWTYQTHT 140
Query: 161 FSNSLEXXXXXXXXXXCQYCIYEVRSRHYATFSSCFLLGALISIGTFNRVTFPAYLILPL 220
FSNS+E + + E ++ + F + +LG + S G FNRVTFPA++ LP
Sbjct: 141 FSNSIETLALISTLTVMEDMVNE-KNIQRSNFKNSVILGLIFSFGVFNRVTFPAFIFLPC 199
Query: 221 LSVFYHCFLSHWIGVAYTAISTALVSALIILFDTKAYSSTDCKSWVIAPLNNLLYNMKVD 280
L +F+ + HW + +S + S L +L DT Y++ K +VI PLNNL YN+ V
Sbjct: 200 LILFWKFYRVHWKSFSLLLLSFSFSSCLFVLIDTNIYNNG--KGFVITPLNNLKYNLNVQ 257
Query: 281 NIAQHGLHPRYTHLLINLPLICGPIILLFI-SQKAVLKLPALSCISGILMLSLFRHQELR 339
N+ HGLHPRYTHLL+NLP I GP++LL I S + KL + ISG+L LS F+HQELR
Sbjct: 258 NLQVHGLHPRYTHLLVNLPQIVGPVLLLAIFSGYKLDKLSTYAIISGLLFLSFFQHQELR 317
Query: 340 FIIPVLPLLCASMNLDNF-DTFFQAETIITSWLVFNIVMGLIMGVFHQAGIIPLIS---Y 395
F++P++PLL ++N T + +WL+FNI+M IMG+ HQAGII + +
Sbjct: 318 FLVPLVPLLVTNLNWTPLSSTLVNKKIFKGTWLLFNIIMAFIMGISHQAGIIQFLGDYFH 377
Query: 396 FSGEEFPVHIWWKTYSPPTWMYSNPDLTVST-TNFKENVEFVDNIPWHVVSNHVVDLKGS 454
F E+ VHIWWKTYSPPTWMY + +LTVS+ N ++ +E +D + + V ++HV+DLKG
Sbjct: 378 FRTEQMGVHIWWKTYSPPTWMYMSNNLTVSSLINTQDGIESIDEVAFSVGNHHVIDLKGC 437
Query: 455 DIELLNKTLTKFSEN--TDSIQLIMPNTVLDKLTPLQSQW--KFITEWETQQHLDLDHID 510
D+ LL +T+ + N + L+ PN++ +L L+ I + HLDLDH+D
Sbjct: 438 DLPLLTETIRRLRLNGSITPLTLVTPNSMTSELKKLKRDGTINLIPKRNYLFHLDLDHLD 497
Query: 511 MPDWTTIKPGLRLYNVSLI 529
D+TT KPGL +Y++ L+
Sbjct: 498 FNDFTTFKPGLTVYSIELL 516
>SPAC4G8.12c [G] KOG4123 Putative alpha 12 mannosyltransferase
Length = 533
Score = 151 bits (381), Expect = 3e-36
Identities = 131/497 (26%), Positives = 210/497 (41%), Gaps = 60/497 (12%)
Query: 47 SYIHPDEHFQTLEPIFXXXXXXXXXXXXEFLPENPCRSITILRLYYTPLLWLNQHIFHXX 106
SYIHPDEH Q+ + EF + P RS+ L + P+ L + I
Sbjct: 26 SYIHPDEHLQSFQIFANKLFGWKVELPWEFTTKKPIRSVVPLNVMLLPIFLLCRCICKSN 85
Query: 107 XXXXXXXXXXXXXXXXTAVVIFFLEFCEVSITHKTKAKFFIRTSYVTWVFQSHTFSNSLE 166
+++I + V + + A +S++ FQ+HTF+NS+E
Sbjct: 86 CSPYPILLFTRLYMCLISLLIDLSIWNIVPLNARWSALLLYSSSFMATTFQTHTFTNSIE 145
Query: 167 XXXXXXXXXXCQYCIYEVRSRHYATFSSCFLLGALISIGTFNRVTFPAYLILPLLSVFYH 226
+ V ++ FLL + +G F R+TF A++I P +
Sbjct: 146 TIFFFLTILFLSK-LNSVPLNKKISYLYTFLLAIVSVLGFFTRITFLAFVIAPYIYFSVR 204
Query: 227 CFLSHWIG--------VAYTAISTALVSALIILFDTKAYSSTDCKSWVIAPLNNLLYNMK 278
CF + + ++S A V A I L D K Y +VI NNL YN +
Sbjct: 205 CFKKNVNNPKDIFLHLCIFVSVSFATVLACI-LEDYKFYGV-----FVITFWNNLKYNSQ 258
Query: 279 VDNIAQHGLHPRYTHLLINLPLICGPIILLFISQKAVLKLPAL-SCISGILMLSLFRHQE 337
++N++QHGLH R TH N+PL+CGP L+F+ + ++ PA + + +LSLF HQE
Sbjct: 259 IENLSQHGLHSRLTHFFTNMPLLCGP--LIFVPKLWDVRKPATWLWLLPVFILSLFPHQE 316
Query: 338 LRFIIPVLPLLCASMNLDNFDTFFQAETIITSWLVFNIVMGLIMGVFHQAGIIPLI---- 393
RF++P AS+ + N ++ I ++++ +V+ + G+ HQ G+IP +
Sbjct: 317 PRFLLP-----AASIFIVNSGCLVRSYWIKFLFVMYAVVLAVFFGIMHQNGVIPAVLEVK 371
Query: 394 ---------------SYFSGEEFPVHIWWKTYSPPTWMYSNPDLTVSTTNFKENVEFVDN 438
YF+ E +WK YS PTWM + P + T+ N
Sbjct: 372 NIIEQRNVTTMENCNLYFNPEMPTTIYFWKIYSAPTWMLARPKFSQINTSHLYNYSTKQM 431
Query: 439 IP--WHVVSNHVVDLKGSDIELLNKTLTKFSENTDSIQLIMPNTVLDKLTPLQSQWKFIT 496
I W V + L + T+F +T L+ P +L + LQ+
Sbjct: 432 ISKFWETYYEEV-----KVVNLPEENTTEFQAST---LLVCPVAMLQTSSYLQNL----- 478
Query: 497 EWETQQHLDLDHIDMPD 513
T H H+D+ D
Sbjct: 479 ---TMLHYIPYHVDLDD 492
>Hs13376765 [G] KOG4123 Putative alpha 12 mannosyltransferase
Length = 579
Score = 97.8 bits (242), Expect = 4e-20
Identities = 107/444 (24%), Positives = 171/444 (38%), Gaps = 90/444 (20%)
Query: 48 YIHPDEHFQTLEPIFXXXXXXXXXXXXEFLPENPCRSITILRLYYTPLLWLNQHIFHXXX 107
Y+HPDE FQ+ E + EF P + CRS+ L WL +
Sbjct: 56 YVHPDEFFQSPEVMAEDILGVQAARPWEFYPSSSCRSVLFPLLISGSTFWLLR--LWEEL 113
Query: 108 XXXXXXXXXXXXXXXTAVVIFFLEFC-EVSITH--------KTKAKFFIRTSYVTWVFQS 158
+++ L F + ++ H + A + SYVT VF +
Sbjct: 114 GPWPGLVSGYALLVGPRLLLTALSFALDGAVYHLAPPMGADRWNALALLSGSYVTLVFYT 173
Query: 159 HTFSNSLEXXXXXXXXXXCQYCIYEVRSRHYATFSS---CFLLGALISIGTFNRVTFPAY 215
TFSN++E + +R +LLG +++ G FNR TF A+
Sbjct: 174 RTFSNTIEGLLFTWLLVLVSSHVTWGPTRKEPAPGPRWRSWLLGGIVAAGFFNRPTFLAF 233
Query: 216 LILPLLSVFYHCFLSHWIGVAYTAISTALVSALIIL----FDTKAYSSTDC--------- 262
++PL +L G + + AL++L + +TD
Sbjct: 234 AVVPL-------YLWGTRGATNPGLKSLTREALVLLPGATLTAAVFVATDSWYFSSPATS 286
Query: 263 KSWVIAPLNNLLYNMKVDNIAQHGLHPRYTHLLINLPLICGPI----------------- 305
++ V+ P+N L YN+ N+A+HG H R THL +N L+ G +
Sbjct: 287 RNLVLTPVNFLHYNLNPQNLARHGTHARLTHLAVNGFLLFGVLHAQALQAAWQQLQVGLQ 346
Query: 306 ------ILLFISQKAVLKLP----ALSCISGILMLSLFRHQELRFIIPV---LPLLCASM 352
+L + +++L P L + +LS F HQE RF+IP+ L LLC+
Sbjct: 347 ASAQMGLLRALGARSLLSSPRSYLLLLYFMPLALLSAFSHQEARFLIPLLVPLVLLCS-- 404
Query: 353 NLDNFDTFFQAETIITSW----LVFNIVMGLIMGVFHQAGIIPLISYFSG-------EEF 401
+T W ++FN + L+ G HQ G++P + Y
Sbjct: 405 ----------PQTQPVPWKGTVVLFNALGALLFGCLHQGGLVPGLEYLEQVVHAPVLPST 454
Query: 402 PVH---IWWKTYSPPTWMYSNPDL 422
P H ++ TY PP + P L
Sbjct: 455 PTHYTLLFTHTYMPPRHLLHLPGL 478
>7291781_1 [G] KOG4123 Putative alpha 12 mannosyltransferase
Length = 701
Score = 70.9 bits (172), Expect = 5e-12
Identities = 97/453 (21%), Positives = 154/453 (33%), Gaps = 106/453 (23%)
Query: 48 YIHPDEHFQTLEPIFXXXXXXXXXXXXEFLPENPCRSITI----LRLYYTPLLWLNQHIF 103
Y+HPDE FQ++E + EF P RSI + LR+ ++ ++ + +
Sbjct: 38 YVHPDEFFQSVEVMTGDHFRLEHTRTWEFNNSLPVRSIVLPFALLRIPWSFYEFVAECLK 97
Query: 104 HXXXXXXXXXXXXXXXXXXTAVVIFFL-EFCEVSITHKTKAKFFIR-----TSYVTWVFQ 157
+I F ++C I +F IR +S++ VF
Sbjct: 98 AWWQLELLGTYAYVVFPRLIYTLISFSNDYCLFRICRLYGLRFEIRLLAMGSSWILLVFG 157
Query: 158 SHTFSNSLEXXXXXXXXXXCQYCIYEVRSRHYA--------------------------- 190
+ TFSNSLE C+ + Y
Sbjct: 158 TRTFSNSLEMAMCSWLLCLVSECMLRTNTVVYKKEFLEEKYDKAESISERVRIWKLKNSL 217
Query: 191 ---TFSSCFLLGALISIGTFNRVTFPAYLILPLLSVFYHC----------FLSHWIGVAY 237
+ + G FNR P +L+ VF+ F + +A
Sbjct: 218 PAHNLQHLMAMSTICVAGVFNR---PTFLLFGAPMVFFWLLRGMGTRSITFRDFNLRIAL 274
Query: 238 TAISTALVSALIILFDTKAYSST----------DCKSWVIAPLNNLLYNMKVDNIAQHGL 287
+ L I D+ Y ++V P N + N+ A HG+
Sbjct: 275 FCLCALPALVLFIFCDSLYYQHLTLGELHMMHLSIDNFVFTPWNFIKANLDSAQTASHGV 334
Query: 288 HPRYTHLLINLPLICGPIILLFISQKAVLKL--------------PALSCISG-----IL 328
HP Y HL++N+P++ + L + A L L +S +SG +
Sbjct: 335 HPCYVHLMVNMPMLFNVLALASLGAFAQLLLRFFRAEYQVLPRFQSIVSLMSGAIFVPLF 394
Query: 329 MLSLFRHQELRFIIPV---LPLLCASMNLDNFDTFFQAET-------------------- 365
LSL HQE RF++PV L LL A + F + +
Sbjct: 395 FLSLINHQEPRFLLPVTFPLILLHAPKLITGFSAKYPFQKDHPLLRLFYDKLLSSKASGP 454
Query: 366 -IITSWLVFNIVMGLIMGVFHQAGIIPLISYFS 397
++ W V N+ + L G HQAG+ PL + S
Sbjct: 455 YLLKIWYVSNVALTLFFGFIHQAGVYPLAADIS 487
>Hs20127468 [MO] KOG1771 GPI-alpha-mannosyltransferase III (GPI10/PIG-B)
involved in glycosylphosphatidylinositol anchor
biosynthesis
Length = 554
Score = 52.4 bits (124), Expect = 2e-06
Identities = 55/261 (21%), Positives = 108/261 (41%), Gaps = 39/261 (14%)
Query: 150 SYVTWVFQSHTFSNSLEXXXXXXXXXXCQYCIYEVRSRHYATFSSCFLLGALISIGTFNR 209
S+ TW + T +N++E Y + +S + +SS L +I
Sbjct: 181 SWFTWYCCTRTLTNTMETVLTIIALFY--YPLEGSKSMNSVKYSSLVALAFIIR------ 232
Query: 210 VTFPAYLILPLLSVFYH---------CFLSHWIGVAYTAISTALVSALIILFDTKAYSST 260
P +IL +F H L H++ V + +S +L+ I
Sbjct: 233 ---PTAVILWTPLLFRHFCQEPRKLDLILHHFLPVGFVTLSLSLMIDRIFF--------- 280
Query: 261 DCKSWVIAPLNNLLYNMKVDNIAQHGLHPRYTHLLINLPLICGPIILLFISQKAVL---- 316
W + N L +N+ + +G HP + + P+I G + FI +
Sbjct: 281 --GQWTLVQFNFLKFNVLQNLGTFYGSHPWHWYFSQGFPVILGTHLPFFIHGCYLAPKRY 338
Query: 317 KLPALSCISGILMLSLFRHQELRFIIPVLP--LLCASMNLDNFDTFFQAETIITSWLVFN 374
++ ++ + +L+ S+ H+E RFI PVLP ++ +L + T+ + ++ + N
Sbjct: 339 RILLVTVLWTLLVYSMLSHKEFRFIYPVLPFCMVFCGYSLTHLKTW--KKPALSFLFLSN 396
Query: 375 IVMGLIMGVFHQAGIIPLISY 395
+ + L G+ HQ G + ++S+
Sbjct: 397 LFLALYTGLVHQRGTLDVMSH 417
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.140 0.446
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,901,881
Number of Sequences: 60738
Number of extensions: 1118405
Number of successful extensions: 3072
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 3044
Number of HSP's gapped (non-prelim): 11
length of query: 530
length of database: 30,389,216
effective HSP length: 111
effective length of query: 419
effective length of database: 23,647,298
effective search space: 9908217862
effective search space used: 9908217862
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)