ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1638 good R KOG4547 General function prediction only WD40 repeat-containing protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1638 580349 582241 631
(631 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR398w [R] KOG4547 WD40 repeat-containing protein 471 e-132
Hs20535866 [R] KOG4547 WD40 repeat-containing protein 100 5e-21
SPAC631.03 [R] KOG4547 WD40 repeat-containing protein 83 1e-15
SPCC1450.03 [R] KOG4547 WD40 repeat-containing protein 59 3e-08
CE28253 [R] KOG4547 WD40 repeat-containing protein 55 3e-07
At5g11240 [R] KOG4547 WD40 repeat-containing protein 54 9e-07
>YDR398w [R] KOG4547 WD40 repeat-containing protein
Length = 643
Score = 471 bits (1213), Expect = e-132
Identities = 262/561 (46%), Positives = 362/561 (63%), Gaps = 39/561 (6%)
Query: 5 SPVVLSQYDPTGQYFAYVSTALDKQRVSVEPTTASKSGLV----NDNFLYLEGKDVCCTC 60
SPV+ S YDP+GQY YV+ ALDKQRV V+PT + S V N+NFLYLE + TC
Sbjct: 3 SPVLQSAYDPSGQYLCYVTVALDKQRVGVQPTQRATSSGVDTVWNENFLYLEDSKLKVTC 62
Query: 61 IQWITY--NDTPLVVLGLNLGEIWLYSPLSNEIVAKLNTGSSLAVLDFQLFSSKDKAWCL 118
++W+ +DT ++LG+N GEIWLYS L+NE+ K TG+S + D L ++ WC+
Sbjct: 63 LKWVNLASSDTVAIILGMNNGEIWLYSVLANEVTYKFTTGNSYEIKDIDLMGNQ--LWCI 120
Query: 119 TGDDTIFLFDMVSQNVSKKFKIDECKDLKKLCIVDSN----RILVASHQIYLIDVISKKL 174
D + FD++ + + F+I+ C L KL IV + ++LVASH I LID+ KK+
Sbjct: 121 DSSDAFYQFDLLQFKLLQHFRINNCVQLNKLTIVPAGDSVAQLLVASHSISLIDIEEKKV 180
Query: 175 LLQYPGHISPVSFLQVTTDGTAFLSASQDDRFLNIYDLDTTKTKSVLVADSNVISISISE 234
++ +PGH+SPVS LQV T+ F+S ++ DRFLN+YD+ + TK VLVA+S++ +S S
Sbjct: 181 VMTFPGHVSPVSTLQVITN-EFFISGAEGDRFLNVYDIHSGMTKCVLVAESDIKELSHSG 239
Query: 235 DWSHVSATTEDGDIAIFEEPLVSINSGNKRRSGKISKQKNKTITLLRSNSATRLPIIKSF 294
++ TTEDG + IF +PLVS S K+R K SK+ +K I ++ S ++PI +F
Sbjct: 240 QADSIAVTTEDGSLEIFVDPLVS--SSTKKRGNK-SKKSSKKIQIV-SKDGRKVPIYNAF 295
Query: 295 INKDLINFSWLENATTPYFDQVQLGNLTDSVTT-LSKPLPSAKSTQTRNNQNSDISSAKL 353
INKDL+N SWL+NAT PYF +Q + + T +S + + R+ D++SA
Sbjct: 296 INKDLLNVSWLQNATMPYFKNLQWREIPNEYTVEISLNWNNKNKSADRDLHGKDLASATN 355
Query: 354 YKEGNATVTSGDNFKHVESIVQS----LDDIEGEG---------ISETLQDRLNIIXXXX 400
Y EGNA VTSGDNFKHV+ ++S L +E E ++ET D+L
Sbjct: 356 YVEGNARVTSGDNFKHVDDAIKSWERELTSLEQEQAKPPQANELLTETFGDKLESSTVAR 415
Query: 401 XXXXXX--------XXANAGTSSVVLAQALKSNDHALLETVLNTRDEKVIQATITRLSPQ 452
GT +V+L+QAL+SNDH+LLETVLN RDE+VI+ TI RL P
Sbjct: 416 ISGKKTNLKGSNLKTATTTGTVTVILSQALQSNDHSLLETVLNNRDERVIRDTIFRLKPA 475
Query: 453 LATILLERLAERIARQTHRQGALNVWVKWCLIIHGGYLVTIPNLINSLSSLHATLRKRAD 512
LA ILLERLAERIARQTHRQG LNVWVKWCLIIHGGYLV+IPNL+++LSSLH+TL++R+D
Sbjct: 476 LAVILLERLAERIARQTHRQGPLNVWVKWCLIIHGGYLVSIPNLMSTLSSLHSTLKRRSD 535
Query: 513 LLPTLMALDTRITRSLDELNS 533
LLP L+ALD R+ ++++ +
Sbjct: 536 LLPRLLALDARLDCTINKFKT 556
>Hs20535866 [R] KOG4547 WD40 repeat-containing protein
Length = 677
Score = 100 bits (250), Expect = 5e-21
Identities = 126/510 (24%), Positives = 215/510 (41%), Gaps = 68/510 (13%)
Query: 67 NDTPLVVLGLNLGEIWLYSPLSNEIVAKLNTGS-SLAVLDFQLFSSKDKAWCLTGDDTIF 125
N T L+ LG +G I LYS + E+ +KL +G V Q + + D I
Sbjct: 92 NQTDLLALGTAVGSILLYSTVKGELHSKLISGGHDNRVNCIQWHQDSGCLYSCSDDKHIV 151
Query: 126 LFDMVSQNVSKKFKIDECKDLKKLCIV-DSNRILVASHQIYLIDVISKKLLLQYPGHISP 184
+++ + V K+K D + LCI D +L A I L + +K++ + GH +P
Sbjct: 152 EWNVQTCKVKCKWKGDN-SSVSSLCISPDGKMLLSAGRTIKLWVLETKEVYRHFTGHATP 210
Query: 185 VSFLQVTT-------------DGTAFLSASQDDRFLNIYDLDT-TKTKSVL----VADSN 226
VS L TT G FLS + DR LN++ + + K KS + V D
Sbjct: 211 VSSLMFTTIRPPNESQPFDGITGLYFLSGAVHDRLLNVWQVRSENKEKSAVMSFTVTDEP 270
Query: 227 V-ISISISEDWSH---VSATTEDGDIAIFE--------EPLVSINSGNKRRSGKISKQKN 274
V I +++SE+ ++ DG + +FE +PL S + GK K
Sbjct: 271 VYIDLTLSENKEEPVKLAVVCRDGQVHLFEHILNGYCKKPLTSNCTIQIATPGKGKKSTP 330
Query: 275 KTITLLRSNSAT-RLPIIKSFINKDLINFSWLENATTPYFDQVQLGN------LTDSVTT 327
K I +L + + ++ ++ L+ SW + P ++V L + L ++
Sbjct: 331 KPIPILAAGFCSDKMSLL-------LVYGSWFQ----PTIERVALNSREPHMCLVRDISN 379
Query: 328 LSKPLPSAKSTQTRN---NQNSDISSAKLYKEGNATVTSGDNFKHVESIVQSLDDIEGEG 384
P T+ R N + + + A + + VES +S G
Sbjct: 380 CWAPKVETAITKVRTPVMNSEAKVLVPGIPGHHAAIKPAPPQTEQVESKRKS------GG 433
Query: 385 ISETLQDRLNIIXXXXXXXXXXXXANAGTSSVVLAQALKSNDHALLETVLNTRDEKVIQA 444
++++RL + + V+L Q L+SND +L VL TR+ +I+
Sbjct: 434 NEVSIEERLGAMDIDTHKKGKEDL-QTNSFPVLLTQGLESNDFEMLNKVLQTRNVNLIKK 492
Query: 445 TITRLSPQLATILLERLAERIARQTHRQGALNVWVKWCLIIHGGYLVTIPNLINSLSSLH 504
T+ R+ LL+ L +R+ + + W+K L +H YL T+P+L+ L +L+
Sbjct: 493 TVLRMPLHTIIPLLQELTKRLQGHPNSAVLMVQWLKCVLTVHASYLSTLPDLVPQLGTLY 552
Query: 505 -------ATLRKRADLLPTLMALDTRITRS 527
T +K + L L+ L T++T S
Sbjct: 553 QLMESRVKTFQKLSHLHGKLILLITQVTAS 582
>SPAC631.03 [R] KOG4547 WD40 repeat-containing protein
Length = 445
Score = 83.2 bits (204), Expect = 1e-15
Identities = 105/431 (24%), Positives = 190/431 (43%), Gaps = 59/431 (13%)
Query: 9 LSQYDPTGQYFAYVSTALDKQRVSVEPTTASKSGLVNDNFLYLEGKDVCCTCIQW----- 63
++ ++ T + +A V ALD QR+ + + A Y+ K+ +CI W
Sbjct: 21 ITAFNETAKLYANVVEALDAQRLRIFDSVAGSLKTE-----YILEKEKVISCIAWEQKPL 75
Query: 64 -----ITYNDT---PLVVLGLNLGEIWLYSPLSNEIVAKLNTGSSLAVLDFQLFSSKDKA 115
IT + + ++VLG N GEI +YS + + G ++ + ++ A
Sbjct: 76 YASEQITTDISGSGEILVLGTNSGEILIYSEHLGSLTRTYSFGILQKIIGAHVLANDGFA 135
Query: 116 WCLTGDDTIFLFDMVSQNVSKKFKI-DECKDLKKLCI--VDSNRILVASHQIYLIDVISK 172
+TG + F + + V F + ++ L + V N L +SH I+++D+ +
Sbjct: 136 IDITGK--VVCFSVNTGEVRTSFTVPSSSREFSGLYLSTVFKNLALASSHNIHIVDLNHR 193
Query: 173 KLLLQYPGHISPVSFL--QVTTDGTAF---LSASQDDRFLNIY--DLD--------TTKT 217
+ H S ++ + Q D F +SA+QD RF+N+Y +LD T K
Sbjct: 194 NPIDSLTTHTSMINSVVFQYLKDENKFYFGVSANQD-RFINLYSKELDGTGEFPTNTVKN 252
Query: 218 KSVLVADSNVISISISEDWSH-----VSATTEDGDIAIFEEPLVSINSGNKRRSGKISKQ 272
LV ++ V +SI+ + + ++A T DG I +FE P +S N + +S +
Sbjct: 253 VGALVCENEVKMLSIAYEIENPETGVLAALTNDGTIEMFENPWLSKLRQNGTNASSLSHR 312
Query: 273 K-----NKTITLLRSNSATRLPIIK---SFINKDLINFSWLENATTPYFDQVQLGNLTDS 324
+ + T+ + S PI+ +F + D + W E+ T F+ V L+
Sbjct: 313 RKLLTSHSTLKICFCRSRGDPPIVLESIAFESTDSLTVVWKESTRT-VFETVPWRILSSQ 371
Query: 325 VTTLSKPLPSAKSTQTRNNQNSDISSAKLYKEGNATVTSGDNFKHVESI--VQSLDDIEG 382
T L +K+ T N+ + + +Y E NAT++SG K + + S + +EG
Sbjct: 372 ATNGLIELVRSKTRLTSKNK----TVSNVYDESNATISSGVMQKDLRKTEEIGSAEAMEG 427
Query: 383 EGISETLQDRL 393
E +L +RL
Sbjct: 428 EEQEPSLAERL 438
>SPCC1450.03 [R] KOG4547 WD40 repeat-containing protein
Length = 240
Score = 58.5 bits (140), Expect = 3e-08
Identities = 36/127 (28%), Positives = 63/127 (49%), Gaps = 4/127 (3%)
Query: 411 AGTSSVVLAQALKSNDHALLETVLNTRDEKVIQATITRLSPQLATILLERLAERIARQTH 470
A T+ ++ ++ LLE LN D V+ T+ + +A L L A +
Sbjct: 2 AATTGNIIKFEENADSKDLLEQCLNCSDVGVVSNTVRNMDGTIAADLARTLIP--AMLIN 59
Query: 471 RQGALNVWVKWCLIIHGGYLVTIPNLINSLSSLHATLRKRADLLPTLMALDTRITR--SL 528
Q +L +W+ W ++ HGGYL T+ +L +SL LH L + A L+ + +L ++ S
Sbjct: 60 MQPSLAIWLHWIIVTHGGYLTTVMDLQDSLVQLHEKLVQSAHLMTKIFSLSGKLNMVLSQ 119
Query: 529 DELNSKR 535
+E+ +R
Sbjct: 120 EEMRQRR 126
>CE28253 [R] KOG4547 WD40 repeat-containing protein
Length = 313
Score = 55.1 bits (131), Expect = 3e-07
Identities = 35/124 (28%), Positives = 61/124 (48%), Gaps = 5/124 (4%)
Query: 409 ANAGTSSVVLAQALKSNDHALLETVLNTRDEKVIQATITRLSPQLATILLERLAERIARQ 468
+ G+ V+L Q + +ND A +++V+ + ++I AT+ L P LL+ + R+ +
Sbjct: 78 SGGGSQCVLLTQGIMANDSAKIDSVIRNLNSEIIHATLRDLQPMQVLPLLKIIESRL--K 135
Query: 469 THRQGALNVWVKWCLI---IHGGYLVTIPNLINSLSSLHATLRKRADLLPTLMALDTRIT 525
T + ++W I IH YL ++PNL + L LR R L+AL +I+
Sbjct: 136 TRNAADIRPTIRWAQIAFSIHMPYLSSLPNLEKEIGGLIGWLRSRVGHHRDLLALHGKIS 195
Query: 526 RSLD 529
D
Sbjct: 196 TIAD 199
>At5g11240 [R] KOG4547 WD40 repeat-containing protein
Length = 606
Score = 53.5 bits (127), Expect = 9e-07
Identities = 60/235 (25%), Positives = 100/235 (42%), Gaps = 31/235 (13%)
Query: 8 VLSQYDPTGQYFAYVSTA------LDKQRVSVEPTTASKSGLVNDNFLYLEGK---DVCC 58
VL+ + P Y A +ST D + V+ A + N GK V
Sbjct: 9 VLTSFSPALDYLA-LSTGDGRIKIWDTVKGQVQTEFADIASTEETNIYTKVGKGHLSVDY 67
Query: 59 TCIQWITYND-------TPLVVLGLNLGEIWLYSPLSNEI---VAKLNTGSSLAVLDFQL 108
TC++W++ T ++VLG G++ S ++ ++ + G AV
Sbjct: 68 TCMKWLSLEKKKKRKLGTSVLVLGTGGGDVLALDVASGQLKWRISDCHPGGVNAV----- 122
Query: 109 FSSKDKAWCLTG---DDTIFLFDMVSQNVSKKFKIDECKDLKKLCIVDSNRILV-ASHQI 164
SS KA C+ D + D S N+ +KFK K + LC+ +ILV AS Q+
Sbjct: 123 -SSSAKASCIYSGGADGMVCQIDPHSGNLIRKFKAST-KTVSSLCVSPDGKILVTASTQL 180
Query: 165 YLIDVISKKLLLQYPGHISPVSFLQVTTDGTAFLSASQDDRFLNIYDLDTTKTKS 219
+ K + ++ GH V + T DG LS++ +R++ ++ D K +S
Sbjct: 181 KTFNCSDLKKIQKFTGHPGVVRCVAFTEDGKYVLSSAVGERYIAVWKTDGAKKQS 235
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.132 0.368
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 31,706,916
Number of Sequences: 60738
Number of extensions: 1248646
Number of successful extensions: 4192
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 4172
Number of HSP's gapped (non-prelim): 8
length of query: 631
length of database: 30,389,216
effective HSP length: 113
effective length of query: 518
effective length of database: 23,525,822
effective search space: 12186375796
effective search space used: 12186375796
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)