ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1674 good K KOG3298 Transcription DNA-directed RNA polymerase subunit E'
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1674 595695 595183 -171
(171 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR404c [K] KOG3298 DNA-directed RNA polymerase subunit E' 273 8e-74
SPACUNK4.06c [K] KOG3298 DNA-directed RNA polymerase subunit E' 218 3e-57
Hs4505947 [K] KOG3298 DNA-directed RNA polymerase subunit E' 155 3e-38
7299973 [K] KOG3298 DNA-directed RNA polymerase subunit E' 151 4e-37
CE22465 [K] KOG3298 DNA-directed RNA polymerase subunit E' 147 9e-36
At5g59180 [K] KOG3298 DNA-directed RNA polymerase subunit E' 146 2e-35
ECU07g1750 [K] KOG3298 DNA-directed RNA polymerase subunit E' 114 6e-26
SPBC2G5.07c [K] KOG3297 DNA-directed RNA polymerase subunit E' 61 8e-10
At4g14660 [K] KOG3298 DNA-directed RNA polymerase subunit E' 58 7e-09
YKL144c [K] KOG3297 DNA-directed RNA polymerase subunit E' 57 9e-09
At3g22900 [K] KOG3298 DNA-directed RNA polymerase subunit E' 55 6e-08
Hs22068810 [K] KOG3297 DNA-directed RNA polymerase subunit E' 48 6e-06
>YDR404c [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 171
Score = 273 bits (698), Expect = 8e-74
Identities = 132/171 (77%), Positives = 153/171 (89%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MFF KDLSLNITLHPSFFGP+MK+YL TKLL+EVEG+CTGKFGYILCVLD +NI+I+RGR
Sbjct: 1 MFFIKDLSLNITLHPSFFGPRMKQYLKTKLLEEVEGSCTGKFGYILCVLDYDNIDIQRGR 60
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
ILPTDGSAEF VKYRAVV+KPFKGEV+DG V SC+ GFEV+VGP+ VFV+ LMP+DLT
Sbjct: 61 ILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGPMKVFVTKHLMPQDLT 120
Query: 121 FNSGSNPPSYQSSEQIITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
FN+GSNPPSYQSSE +IT SR+R+KI G S+V+SI+AIGSIKEDYLG I
Sbjct: 121 FNAGSNPPSYQSSEDVITIKSRIRVKIEGCISQVSSIHAIGSIKEDYLGAI 171
>SPACUNK4.06c [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 172
Score = 218 bits (555), Expect = 3e-57
Identities = 97/170 (57%), Positives = 134/170 (78%)
Query: 2 FFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGRI 61
FF K+LSL I+LHPS+FGP+M+ YL KLL +VEGTC+G++GYI+CVLD I+I++GR+
Sbjct: 3 FFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRV 62
Query: 62 LPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLTF 121
+P G AEF VKYRAV+W+PF+GEV+D IV++ +GF +GPL+VFVS L+P D+ F
Sbjct: 63 VPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKF 122
Query: 122 NSGSNPPSYQSSEQIITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
+ +NPP+Y +Q+I KGS VRLKIVG R++ I+AI ++KEDYLGV+
Sbjct: 123 DPTANPPNYSGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL 172
>Hs4505947 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 172
Score = 155 bits (391), Expect = 3e-38
Identities = 74/173 (42%), Positives = 112/173 (63%), Gaps = 4/173 (2%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MF+ L I LHP +FGP + + KL EVEGTCTGK+G+++ V +NI G
Sbjct: 1 MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGA--GV 58
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
I P G + VKY+A+V++PFKGEV+D +V+ +G E+GP+S F+S +P ++
Sbjct: 59 IQPGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEME 118
Query: 121 FNSGSNPPSYQSSEQ--IITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
F+ SNPP Y++ ++ +I + +RLKIVG R + N I+AIGS+ +DYLG++
Sbjct: 119 FDPNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLV 171
>7299973 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 552
Score = 151 bits (382), Expect = 4e-37
Identities = 73/167 (43%), Positives = 107/167 (63%), Gaps = 4/167 (2%)
Query: 7 LSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGRILPTDG 66
L I LHP +FGPQ+ + KL EVEGTCTGK+G+++ V + +I G I P G
Sbjct: 386 LEQEILLHPRYFGPQLLETVKQKLYSEVEGTCTGKYGFVIAVTTID--QIGSGVIQPGQG 443
Query: 67 SAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLTFNSGSN 126
+ VKY+A+V++PFKGEVLD +V +G E+GPLS F+S +P D+ F N
Sbjct: 444 FVVYPVKYKAIVFRPFKGEVLDAVVKQINKVGMFAEIGPLSCFISHHSIPADMQFCPNGN 503
Query: 127 PPSYQSSEQ--IITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
PP Y+S ++ +I+ ++RLKIVG R + I+AIG++ +DYLG++
Sbjct: 504 PPCYKSKDEDVVISGEDKIRLKIVGTRVDATGIFAIGTLMDDYLGLV 550
>CE22465 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 197
Score = 147 bits (370), Expect = 9e-36
Identities = 66/172 (38%), Positives = 109/172 (63%), Gaps = 4/172 (2%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MFF L + LHP +FGP + + KL EVEGTCTGK+G+++ V + I G
Sbjct: 24 MFFHLSLDHEVCLHPKYFGPNLNETIKMKLFNEVEGTCTGKYGFVIAVTTIDTIG--HGL 81
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
I P G + V+Y+A+V++PFKG+V+DG+V+ +G ++GPLS F+S +P D+
Sbjct: 82 IQPGRGFVIYPVRYKAIVFRPFKGQVVDGVVTQVNKVGIFCDIGPLSCFISRHCIPPDME 141
Query: 121 FNSGSNPPSYQSSEQ--IITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGV 170
F+ S P Y+++++ +I +R+K++G R + N I+AIG++ +D+LG+
Sbjct: 142 FDPNSEKPCYKTNDEANVIRNDDEIRVKLIGTRVDANDIFAIGTLMDDFLGL 193
>At5g59180 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 176
Score = 146 bits (368), Expect = 2e-35
Identities = 73/173 (42%), Positives = 114/173 (65%), Gaps = 5/173 (2%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MFF L N+ LHP FFG +K L++KL+++VEGTC+G+ G+++ + + I +G
Sbjct: 1 MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIG--KGL 58
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
I G F VKY+ VV++PFKGE+L+ +V+ +GF E GP+ +FVS L+P+D+
Sbjct: 59 IRDGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDME 118
Query: 121 FNSGSNPPSYQSSEQI--ITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
F +G + P+Y +S+ I K VRLKI+G R + +I+ +G+IK+D+LGVI
Sbjct: 119 FQAG-DMPNYTTSDGSVKIQKECEVRLKIIGTRVDATAIFCVGTIKDDFLGVI 170
>ECU07g1750 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 155
Score = 114 bits (285), Expect = 6e-26
Identities = 67/171 (39%), Positives = 96/171 (55%), Gaps = 17/171 (9%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MFF +D NI L P++ GP ++ + LL +VEG+C+ GY++ VL + EI R
Sbjct: 1 MFFVRDFVQNIHLSPNYLGPNIQALIEEYLLSKVEGSCSSS-GYVVMVLSID--EISESR 57
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
I+ T G FTVKY+A+ KP KGEV+D V +G VGPL+VF+S +P L
Sbjct: 58 IILT-GETIFTVKYKALTLKPLKGEVIDANVVETNKMGVFASVGPLTVFISNHQIPNFLL 116
Query: 121 FNSGSNPPSYQSSEQIITKGSRVRLKIVGARSEVNSIYAIGSIKEDYLGVI 171
N ITK +RLKI+G + + IYA+G++ +D LG+I
Sbjct: 117 ENE-------------ITKNVMIRLKIIGTKIDSTRIYAVGTLNDDSLGII 154
>SPBC2G5.07c [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 203
Score = 60.8 bits (146), Expect = 8e-10
Identities = 36/122 (29%), Positives = 60/122 (48%), Gaps = 6/122 (4%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MF S I++HPS F K L ++ ++ G +CV D ++I G
Sbjct: 1 MFLLSRFSDIISIHPSNFWKPTKEALAEEIHKKYANKVIQNIGLAICVYDF--LKIGEGI 58
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
I DGS+ V +R ++++PF+GEV+ G + SC+ G V + F +P+D+
Sbjct: 59 IKYGDGSSYMNVVFRLIIFRPFRGEVMLGKIKSCSEEGIRVTIS----FFDDIFIPKDML 114
Query: 121 FN 122
F+
Sbjct: 115 FD 116
>At4g14660 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 161
Score = 57.8 bits (138), Expect = 7e-09
Identities = 45/155 (29%), Positives = 75/155 (48%), Gaps = 7/155 (4%)
Query: 19 GPQMKRYLMTKLLQEVEGT-CTGKFGYILCVLDCENIEIERGRILPTDGSAEFTVKYRAV 77
G +KR ++ +LL+ T + GY + V + +I G+I G F V + +
Sbjct: 5 GLMLKRAILVELLEAFASKKATKELGYYVAVTTLD--KIGEGKIREHTGEVLFPVMFSGM 62
Query: 78 VWKPFKGEVLDGIVSSCTNLGFEVEVGPL-SVFVSPFLMPEDLTFNSGSNPPSYQSSEQI 136
+K FKGE++ G+V G + GP+ +V++S MP D + G NP
Sbjct: 63 TFKIFKGEIIHGVVHKVLKHGVFMRCGPIENVYLSYTKMP-DYKYIPGENPIFMNEKTSR 121
Query: 137 ITKGSRVRLKIVGAR-SEV-NSIYAIGSIKEDYLG 169
I + VR+ ++G + EV A+ S++ DYLG
Sbjct: 122 IQVETTVRVVVIGIKWMEVEREFQALASLEGDYLG 156
>YKL144c [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 212
Score = 57.4 bits (137), Expect = 9e-09
Identities = 29/76 (38%), Positives = 48/76 (63%), Gaps = 3/76 (3%)
Query: 46 LCVLDCENIEIERGRILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGP 105
LC+ + + +E G++ P DGS+ V +RAVV+KPF GE++ G +S CT G +V +
Sbjct: 44 LCITIYDLLTVEEGQLKPGDGSSYINVTFRAVVFKPFLGEIVTGWISKCTAEGIKVSL-- 101
Query: 106 LSVFVSPFLMPEDLTF 121
L +F F+ P+++ F
Sbjct: 102 LGIFDDIFI-PQNMLF 116
>At3g22900 [K] KOG3298 DNA-directed RNA polymerase subunit E'
Length = 174
Score = 54.7 bits (130), Expect = 6e-08
Identities = 41/154 (26%), Positives = 66/154 (42%), Gaps = 5/154 (3%)
Query: 19 GPQMKRYLMTKLLQEV-EGTCTGKFGYILCVLDCENIEIERGRILPTDGSAEFTVKYRAV 77
G ++R ++ +LL+ + T GY++ ENI G+I G +F V + +
Sbjct: 21 GLMLQRAIVIRLLEAFSKEKATKDLGYLITPTILENIG--EGKIKEQTGEIQFPVVFNGI 78
Query: 78 VWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLTFNSGSNPPSYQSSEQII 137
+K FKGE++ G+V G ++ GP + + F G NP I
Sbjct: 79 CFKMFKGEIVHGVVHKVHKTGVFLKSGPYEIIYLSHMKMPGYEFIPGENPFFMNQYMSRI 138
Query: 138 TKGSRVRLKIVGA--RSEVNSIYAIGSIKEDYLG 169
G+RVR ++ R A+ SI D LG
Sbjct: 139 QIGARVRFVVLDTEWREAEKDFMALASIDGDNLG 172
>Hs22068810 [K] KOG3297 DNA-directed RNA polymerase subunit E'
Length = 204
Score = 48.1 bits (113), Expect = 6e-06
Identities = 32/136 (23%), Positives = 61/136 (44%), Gaps = 10/136 (7%)
Query: 1 MFFFKDLSLNITLHPSFFGPQMKRYLMTKLLQEVEGTCTGKFGYILCVLDCENIEIERGR 60
MF ++ + + P F ++ + +L +++ G +C+ D ++E
Sbjct: 1 MFVLVEMVDTVRIPPWQFERKLNDSIAEELNKKLANKVVYNVGLCICLFDIT--KLEDAY 58
Query: 61 ILPTDGSAEFTVKYRAVVWKPFKGEVLDGIVSSCTNLGFEVEVGPLSVFVSPFLMPEDLT 120
+ P DG++ V +R VV+ PF E+L G + C+ G V +G F L+P +
Sbjct: 59 VFPGDGASHTKVHFRCVVFHPFLDEILIGKIKGCSPEGVHVSLG----FFDDILIPPE-- 112
Query: 121 FNSGSNPPSYQSSEQI 136
S P + +EQ+
Sbjct: 113 --SLQQPAKFDEAEQV 126
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.141 0.416
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,385,910
Number of Sequences: 60738
Number of extensions: 416780
Number of successful extensions: 784
Number of sequences better than 1.0e-05: 12
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 765
Number of HSP's gapped (non-prelim): 12
length of query: 171
length of database: 30,389,216
effective HSP length: 99
effective length of query: 72
effective length of database: 24,376,154
effective search space: 1755083088
effective search space used: 1755083088
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)