ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1706 good E KOG2551 Amino acid transport and metabolism Phospholipase/carboxyhydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1706 606430 607083 218
(218 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase 280 1e-75
YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase 201 6e-52
SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase 96 4e-20
YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase 91 2e-18
SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase 87 1e-17
At5g65400 [E] KOG2551 Phospholipase/carboxyhydrolase 72 4e-13
7300631 [E] KOG2551 Phospholipase/carboxyhydrolase 68 8e-12
At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase 68 8e-12
Hs18201880 [E] KOG2551 Phospholipase/carboxyhydrolase 62 6e-10
CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase 60 2e-09
At4g24380 [E] KOG2551 Phospholipase/carboxyhydrolase 57 1e-08
>YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 223
Score = 280 bits (715), Expect = 1e-75
Identities = 137/220 (62%), Positives = 166/220 (75%), Gaps = 5/220 (2%)
Query: 1 MSKKVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPADLPSDVADIGA- 59
M+K VLMLHGLAQSG YF SKTKGFR +E LGY+ +YPTAP PAD+P + ++ A
Sbjct: 1 MTKNVLMLHGLAQSGDYFASKTKGFRAEMEKLGYKLYYPTAPNEFPPADVPDFLGEVIAD 60
Query: 60 ASGDDYH----AWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYLM 115
A GD + AWL+ DP G Y +P T+ +L YV+ENGPF GI GFSQGAGV GYL
Sbjct: 61 APGDGENTGVLAWLENDPSTGGYFIPQTTIDYLHNYVLENGPFAGIVGFSQGAGVAGYLA 120
Query: 116 TDFNNLLGLTEEQQPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLDTVTESS 175
TDFN LLGLT E+QP ++FF+A SGFRF+P+ YQEQY HPI+V SLHVQG+LDT+TE +
Sbjct: 121 TDFNGLLGLTTEEQPPLEFFMAVSGFRFQPQQYQEQYDLHPISVPSLHVQGELDTITEPA 180
Query: 176 QVKGLYTSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWLQ 215
+V+GLY SC E + T L HSGGHFVPNS+GFV K+A WLQ
Sbjct: 181 KVQGLYNSCTEDSRTLLMHSGGHFVPNSRGFVRKVAQWLQ 220
>YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 266
Score = 201 bits (511), Expect = 6e-52
Identities = 101/223 (45%), Positives = 141/223 (62%), Gaps = 11/223 (4%)
Query: 3 KKVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPADLPSDVADIG---- 58
KKVLMLHG QS F +KT G R+ L+ LGY+ +YP AP ++ L ++ G
Sbjct: 5 KKVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAPHSIDKKALFQSESEKGRDAA 64
Query: 59 -----AASGDDYHAWLQPDP-LHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTG 112
+A+ D+ + W +P +++ +L+ YV+ENGPFDG+ GFSQGAG+ G
Sbjct: 65 KEFNTSATSDEVYGWFFRNPESFNSFQIDQKVFNYLRNYVLENGPFDGVIGFSQGAGLGG 124
Query: 113 YLMTDFNNLLGLTEEQQPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLDTVT 172
YL+TDFN +L LT+EQQPA+KFFI+FSGF+ + YQ++Y I V SLHV+G+LD V
Sbjct: 125 YLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSYQKEY-HRIIQVPSLHVRGELDEVV 183
Query: 173 ESSQVKGLYTSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWLQ 215
S++ LY S + T L H G HFVPNSK FV ++ +W+Q
Sbjct: 184 AESRIMALYESWPDNKRTLLVHPGAHFVPNSKPFVSQVCNWIQ 226
>SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 233
Score = 95.9 bits (237), Expect = 4e-20
Identities = 70/224 (31%), Positives = 100/224 (44%), Gaps = 15/224 (6%)
Query: 1 MSK--KVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPADLPSD----- 53
MSK KVL LHG QSGP F K ++ L E +PT P+ P+D
Sbjct: 1 MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYA-ELHFPTGPVVADEEADPNDEEEKK 59
Query: 54 -VADIGAASGDDYHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTG 112
+A +G W + + Y D +L+ + +Y+ E GPFDG+ GFSQGAG+
Sbjct: 60 RLAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGA 119
Query: 113 YLMTDFN-NLLGLTEEQQPAIKFFIAFSGFRF-RPEIYQEQYIQHPITVRSLHVQGQLDT 170
L Q P KF + GFR +PE + + +T SLH+ G DT
Sbjct: 120 MLAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEF--DHFYNPKLTTPSLHIAGTSDT 177
Query: 171 VTESSQVKGLYTSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWL 214
+ ++ K L C+ + L H G H VP + I D++
Sbjct: 178 LVPLARSKQLVERCE--NAHVLLHPGQHIVPQQAVYKTGIRDFM 219
>YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 243
Score = 90.5 bits (223), Expect = 2e-18
Identities = 65/237 (27%), Positives = 104/237 (43%), Gaps = 29/237 (12%)
Query: 4 KVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPADLPSDVAD---IGAA 60
K+L LHG Q+G F K+ G R++L+ + Y AP+ L DLP ++ D
Sbjct: 7 KLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDAPVLLEKKDLPFEMDDEKWQATL 66
Query: 61 SGDDYHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYLMTDFNN 120
D AW + E + + LK + +++ NGP+DGI GFSQGA ++ + +
Sbjct: 67 DADVNRAWFYHSEISHELDISE-GLKSVVDHIKANGPYDGIVGFSQGAALSSIITNKISE 125
Query: 121 LLGLTEEQQPAIKFFIAFSGFRFR------------PEIYQEQYIQHP-ITVRSLHVQGQ 167
L+ P K + SG+ F E +++ + P + + + + G
Sbjct: 126 LV----PDHPQFKVSVVISGYSFTEPDPEHPGELRITEKFRDSFAVKPDMKTKMIFIYGA 181
Query: 168 LDTVTESSQVKGLY-------TSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWLQNS 217
D S + K LY KE + EH GGH VPN K + I + + +S
Sbjct: 182 SDQAVPSVRSKYLYDIYLKAQNGNKEKVLAY-EHPGGHMVPNKKDIIRPIVEQITSS 237
>SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 429
Score = 87.4 bits (215), Expect = 1e-17
Identities = 67/225 (29%), Positives = 102/225 (44%), Gaps = 24/225 (10%)
Query: 4 KVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPA---------DLPSDV 54
K+L +HG A+SG F K + R + +F++PT PI L A D S V
Sbjct: 8 KILCIHGYAESGELFSVKLRALRERMAD-SVDFYFPTGPIELDKAKDELNGSGFDALSTV 66
Query: 55 ADIGAASGDDYHAWLQPDPLHGEYRL-PDVTLKFLKEYVVENGPFDGICGFSQGAGVTGY 113
AS W + + +L P ++L Y+ E+GPFDGI GFSQG +
Sbjct: 67 FSSSPAS--HRRGWWRINEYADTKQLEPTKAFEYLASYIKEHGPFDGILGFSQGTNLAAN 124
Query: 114 LMTDFNNLLGLTEEQ----QPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLD 169
L L+ + + Q QP +F + FSG+ FRP + + + +LH+ G+ D
Sbjct: 125 LAA----LVTIPKYQEYFSQPPFRFALFFSGY-FRPLLMDGAVHATKLDLPTLHLLGKYD 179
Query: 170 TVTESSQVKGLYTSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWL 214
TV + L +CK+ F H H +P +V AD++
Sbjct: 180 TVLSTETSTTLVRACKDAQVLF--HPAAHQIPAPHAYVEPAADFI 222
>At5g65400 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 234
Score = 72.4 bits (176), Expect = 4e-13
Identities = 62/204 (30%), Positives = 93/204 (45%), Gaps = 20/204 (9%)
Query: 4 KVLMLHGLAQSGPYFESKT-KGFRRVLEPLGYEFFYPTAPINLSPADLPSDVADIGAASG 62
++L LHG SG ++ K +L L +F AP PA SDV
Sbjct: 13 RILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFL--DAPF---PATGKSDVERF---FD 64
Query: 63 DDYHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYLMTDFNNLL 122
Y+ W Q + EYR + L ++++Y+++NGPFDG+ GFSQGA +T +
Sbjct: 65 PPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGS 124
Query: 123 GLTEEQQPAIKFFIAFSGFRFRPEIYQE-----QYIQHPITVRSLHVQGQLDTVTESSQV 177
LT + P +KF + SG + ++ E P+ SLH G+ D + +V
Sbjct: 125 ALT--KVPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEV 182
Query: 178 KGLYTSCKEGTSTFLEHSGGHFVP 201
L S E + HSGGH +P
Sbjct: 183 --LVESFVE--PVVIHHSGGHIIP 202
>7300631 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 279
Score = 68.2 bits (165), Expect = 8e-12
Identities = 61/205 (29%), Positives = 93/205 (44%), Gaps = 22/205 (10%)
Query: 4 KVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPI---NLSPADLPSDVADIGAA 60
+VL LHG Q+G F++K FR+ EF + TAP L A P A
Sbjct: 32 RVLCLHGYRQNGEAFKNKLGSFRKFANKYA-EFVFITAPHVAKALESAAEPVPEQRSWWA 90
Query: 61 SGDD--YHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYLMTDF 118
+ DD + + P G +L+ ++E GPF G+ GFSQGA G
Sbjct: 91 NKDDGSFKGTNKGGPAFGFQE----SLRCVEEAWRTQGPFQGLLGFSQGACFVGL----- 141
Query: 119 NNLLGLTEEQQPAIK--FFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLDTVTESSQ 176
+ GL +++ +I+ F + SGF ++ Y + I++ +LH+ GQ D +
Sbjct: 142 --ICGLAKKKLTSIRPEFAVLASGFLSGSLVHMSAY-EEAISIPTLHIYGQTDEIIPKEM 198
Query: 177 VKGLYTSCKEGTSTFLEHSGGHFVP 201
+ L K + LEHSGGH+ P
Sbjct: 199 SESLAARFK--NAEVLEHSGGHYFP 221
>At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 229
Score = 68.2 bits (165), Expect = 8e-12
Identities = 68/234 (29%), Positives = 105/234 (44%), Gaps = 34/234 (14%)
Query: 4 KVLMLHGLAQSGPYFESKTKGFRRVLE----------PLGYEFFYPTAP-----INLSPA 48
++L LHG Q+ F+ +T + L+ P +F Y TA N A
Sbjct: 3 RILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCNKKFA 62
Query: 49 DLPSDVADIGAASGDDYHAWLQPDPLHGEYRLP--DVTLKFLKEYVVENGPFDGICGFSQ 106
L S D + +G A Q DPL + + D +L +LK E GPFDGI GFSQ
Sbjct: 63 WLVSSDFDKPSETGWTV-AQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFSQ 121
Query: 107 GAGVTGYLMTDFNNLLGLTEEQQPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQG 166
GA + + L+G + +F + SGF P + ++ + I SLH+ G
Sbjct: 122 GAAMAAAVCGKQEQLVGEID-----FRFCVLCSGFTPWPLLEMKE--KRSIKCPSLHIFG 174
Query: 167 -----QLDTVTE-SSQVKGLYTSCKEGTSTFLEHSGGHFVPNSKGFVLKIADWL 214
VT+ SS + GL+ ++G +T +EH GH +P ++ +I +L
Sbjct: 175 SQPGKDRQIVTQASSDLAGLF---EDGCATIVEHDFGHIIPTKSPYIDEIKAFL 225
>Hs18201880 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 227
Score = 62.0 bits (149), Expect = 6e-10
Identities = 42/107 (39%), Positives = 57/107 (53%), Gaps = 8/107 (7%)
Query: 96 GPFDGICGFSQGAGVTGYLMTDFNNLLGLTEEQQ-PAIKFFIAFSGFRFRPEIYQEQYIQ 154
GPFDG+ GFSQGA + + LG + + P +F + SGF R ++E +Q
Sbjct: 110 GPFDGLLGFSQGAALAALVCA-----LGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQ 164
Query: 155 HPITVRSLHVQGQLDTVTESSQVKGLYTSCKEGTSTFLEHSGGHFVP 201
P+++ SLHV G D V S + L S G T L HSGGHF+P
Sbjct: 165 RPLSLPSLHVFGDTDKVIPSQESVQL-ASQFPGAIT-LTHSGGHFIP 209
>CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 221
Score = 60.5 bits (145), Expect = 2e-09
Identities = 54/212 (25%), Positives = 91/212 (42%), Gaps = 8/212 (3%)
Query: 4 KVLMLHGLAQSGPYFESKTKGFRRVLEPLGYEFFYPTAPINLSPADLPSDVADIGAASGD 63
++L LHG Q F KT R++++ L EF + ++ D D + S +
Sbjct: 8 RILCLHGYRQCDQSFRQKTGSTRKLVKSLA-EFEFVNG-VHSVAVDEHVDSSRAWWFSNN 65
Query: 64 DYHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYLMTDFNNLLG 123
+ ++ + + +K ++ ENGPFDG+ GFSQGA + L+
Sbjct: 66 EAMSFSSRESTEVAVGFEESVAAVVK-FIEENGPFDGLLGFSQGASMVHLLIAKAQ---- 120
Query: 124 LTEEQQPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLDTVTESSQVKGLYTS 183
L E + P I+F I FSGF + + S+HV G D + + + +
Sbjct: 121 LGEIKLPGIRFAIFFSGFLSLSSKHDSLTLLRIKEFPSMHVFGDADEIVARPKSEKM-AD 179
Query: 184 CKEGTSTFLEHSGGHFVPNSKGFVLKIADWLQ 215
+ + H GGH VP+ KIA +++
Sbjct: 180 MFDVEPLRIAHDGGHVVPSMSKHKEKIAGFMR 211
>At4g24380 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 208
Score = 57.4 bits (137), Expect = 1e-08
Identities = 41/148 (27%), Positives = 68/148 (45%), Gaps = 8/148 (5%)
Query: 55 ADIGAASGDDYHAWLQPDPLHGEYRLPDVTLKFLKEYVVENGPFDGICGFSQGAGVTGYL 114
+D+ Y+ W Q + EY + L++L++ +++ GPFDG+ GFSQGA ++G L
Sbjct: 31 SDVEGIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGL 90
Query: 115 MTDFNNLLGLTEEQQPAIKFFIAFSGFRFRPEIYQEQYIQHPITVRSLHVQGQLDTVTE- 173
G+ ++ P IKF I G + + E + SLH G+ D +
Sbjct: 91 --PGLQAKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPY 148
Query: 174 SSQVKGLYTSCKEGTSTFLEHSGGHFVP 201
+Q+ Y + + H GH VP
Sbjct: 149 GTQLIESYKN-----PVVVHHPKGHTVP 171
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.139 0.424
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,458,862
Number of Sequences: 60738
Number of extensions: 654259
Number of successful extensions: 1247
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1225
Number of HSP's gapped (non-prelim): 11
length of query: 218
length of database: 30,389,216
effective HSP length: 102
effective length of query: 116
effective length of database: 24,193,940
effective search space: 2806497040
effective search space used: 2806497040
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)