ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1710 good E KOG2551 Amino acid transport and metabolism Phospholipase/carboxyhydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1710 607384 608166 261
(261 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase 239 2e-63
YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase 203 2e-52
SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase 119 3e-27
SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase 115 8e-26
YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase 96 7e-20
CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase 84 2e-16
Hs18201880 [E] KOG2551 Phospholipase/carboxyhydrolase 84 3e-16
7300631 [E] KOG2551 Phospholipase/carboxyhydrolase 72 1e-12
At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase 71 1e-12
At5g65400 [E] KOG2551 Phospholipase/carboxyhydrolase 65 7e-11
At4g24380 [E] KOG2551 Phospholipase/carboxyhydrolase 59 9e-09
>YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 266
Score = 239 bits (611), Expect = 2e-63
Identities = 125/270 (46%), Positives = 171/270 (63%), Gaps = 13/270 (4%)
Query: 1 MSTSKKVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFNDSEVDL- 59
MS KKVLMLHG+ QSD IF AKT G+RK L+K GY+L YPC P+ + K SE +
Sbjct: 1 MSEKKKVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAPHSIDKKALFQSESEKG 60
Query: 60 RDSEK-------STGMYGWFLKDPET-HSYTLEPELLEYLAQYIKENGPFDGIGGFSQGA 111
RD+ K S +YGWF ++PE+ +S+ ++ ++ YL Y+ ENGPFDG+ GFSQGA
Sbjct: 61 RDAAKEFNTSATSDEVYGWFFRNPESFNSFQIDQKVFNYLRNYVLENGPFDGVIGFSQGA 120
Query: 112 GLAGYLSTDLWSILPLNKEEQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGEL 171
GL GYL TD IL L E+QP KF + FSGFK E + +Q Y IQ+ +LH+ GEL
Sbjct: 121 GLGGYLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSYQKEYH-RIIQVPSLHVRGEL 179
Query: 172 DSLVTEERSMKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWLHAQADENLRELSGS 231
D +V E R M L+E+ + RT++ H G H++PN+KPF++ + +W+ + +E +
Sbjct: 180 DEVVAESRIMALYESWPDNKRTLLVHPGAHFVPNSKPFVSQVCNWIQGITSKEGQEHNAQ 239
Query: 232 EESAMKNTVNNSDPQLDDDLLDMIDSFGKI 261
E K PQL+DDLLDMIDS GK+
Sbjct: 240 PEVDRKQF---DKPQLEDDLLDMIDSLGKL 266
>YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 223
Score = 203 bits (516), Expect = 2e-52
Identities = 96/218 (44%), Positives = 137/218 (62%), Gaps = 4/218 (1%)
Query: 4 SKKVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFND--SEV--DL 59
+K VLMLHG +QS F +KT G R +++K GY+L YP P + D D EV D
Sbjct: 2 TKNVLMLHGLAQSGDYFASKTKGFRAEMEKLGYKLYYPTAPNEFPPADVPDFLGEVIADA 61
Query: 60 RDSEKSTGMYGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAGYLST 119
++TG+ W DP T Y + ++YL Y+ ENGPF GI GFSQGAG+AGYL+T
Sbjct: 62 PGDGENTGVLAWLENDPSTGGYFIPQTTIDYLHNYVLENGPFAGIVGFSQGAGVAGYLAT 121
Query: 120 DLWSILPLNKEEQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELDSLVTEER 179
D +L L EEQPP +F M SGF+F+P+Q+Q Y+ HPI + +LH+ GELD++ +
Sbjct: 122 DFNGLLGLTTEEQPPLEFFMAVSGFRFQPQQYQEQYDLHPISVPSLHVQGELDTITEPAK 181
Query: 180 SMKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWL 217
L+ +C +RT++ HSGGH++PN++ F+ + WL
Sbjct: 182 VQGLYNSCTEDSRTLLMHSGGHFVPNSRGFVRKVAQWL 219
>SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 233
Score = 119 bits (299), Expect = 3e-27
Identities = 80/210 (38%), Positives = 110/210 (52%), Gaps = 10/210 (4%)
Query: 1 MSTSKKVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSK-DFNDSEVDL 59
MS KVL LHG+ QS +F K ++K L K EL +P GP + D ND E
Sbjct: 1 MSKPLKVLCLHGWIQSGPVFSKKMGSVQKYLSKYA-ELHFPTGPVVADEEADPNDEEEKK 59
Query: 60 R----DSEKSTGMYGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAG 115
R E++ G +GWF + ++Y E LE + QY++E GPFDG+ GFSQGAG+
Sbjct: 60 RLAALGGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGA 119
Query: 116 YLSTDLWSILPLNKEEQ-PPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELDSL 174
L+ L P N Q PPFKF ++ GF+ E +F Y + +LHI G D+L
Sbjct: 120 MLAQMLQPGQPPNPYVQHPPFKFVVFVGGFRAEKPEFDHFYNP-KLTTPSLHIAGTSDTL 178
Query: 175 VTEERSMKLFEACDPSTRTMVKHSGGHYIP 204
V RS +L E C+ ++ H G H +P
Sbjct: 179 VPLARSKQLVERCE--NAHVLLHPGQHIVP 206
>SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 429
Score = 115 bits (287), Expect = 8e-26
Identities = 73/218 (33%), Positives = 116/218 (52%), Gaps = 15/218 (6%)
Query: 2 STSKKVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSK--DFNDSEVD- 58
+T K+L +HGY++S +F K +R+++ + +P GP +L + N S D
Sbjct: 4 ATKSKILCIHGYAESGELFSVKLRALRERMADS-VDFYFPTGPIELDKAKDELNGSGFDA 62
Query: 59 ---LRDSEKSTGMYGWFLKDPETHSYTLEP-ELLEYLAQYIKENGPFDGIGGFSQGAGLA 114
+ S ++ GW+ + + LEP + EYLA YIKE+GPFDGI GFSQG LA
Sbjct: 63 LSTVFSSSPASHRRGWWRINEYADTKQLEPTKAFEYLASYIKEHGPFDGILGFSQGTNLA 122
Query: 115 GYLSTDLWSILPLNKE--EQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELD 172
L+ + +P +E QPPF+FA++FSG+ F P + L TLH++G+ D
Sbjct: 123 ANLAALV--TIPKYQEYFSQPPFRFALFFSGY-FRPLLMDGAVHATKLDLPTLHLLGKYD 179
Query: 173 SLVTEERSMKLFEACDPSTRTMVKHSGGHYIPNTKPFI 210
++++ E S L AC + ++ H H IP ++
Sbjct: 180 TVLSTETSTTLVRACKDA--QVLFHPAAHQIPAPHAYV 215
>YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 243
Score = 95.5 bits (236), Expect = 7e-20
Identities = 69/239 (28%), Positives = 112/239 (45%), Gaps = 26/239 (10%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKD--FNDSEVDLRDSE 63
K+L LHG+ Q+ +F K++G+RK L+K + Y P L KD F + + +
Sbjct: 7 KLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDAPVLLEKKDLPFEMDDEKWQATL 66
Query: 64 KSTGMYGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAGYLSTDLWS 123
+ WF +H + E L+ + +IK NGP+DGI GFSQGA L+ ++ +
Sbjct: 67 DADVNRAWFYHSEISHELDIS-EGLKSVVDHIKANGPYDGIVGFSQGAALSSIITNKISE 125
Query: 124 ILPLNKEEQPPFKFAMYFSGFKF-EP-----------EQFQAPYETHP-IQLKTLHIVGE 170
++P + P FK ++ SG+ F EP E+F+ + P ++ K + I G
Sbjct: 126 LVP----DHPQFKVSVVISGYSFTEPDPEHPGELRITEKFRDSFAVKPDMKTKMIFIYGA 181
Query: 171 LDSLVTEERSMKLFEAC------DPSTRTMVKHSGGHYIPNTKPFINMILSWLHAQADE 223
D V RS L++ + +H GGH +PN K I I+ + + E
Sbjct: 182 SDQAVPSVRSKYLYDIYLKAQNGNKEKVLAYEHPGGHMVPNKKDIIRPIVEQITSSLQE 240
>CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 221
Score = 84.0 bits (206), Expect = 2e-16
Identities = 61/229 (26%), Positives = 100/229 (43%), Gaps = 27/229 (11%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFNDSEVDLRDSEKS 65
++L LHGY Q D FR KT RK L K E + G + + + DS
Sbjct: 8 RILCLHGYRQCDQSFRQKTGSTRK-LVKSLAEFEFVNGVHSVAVDEHVDSS--------- 57
Query: 66 TGMYGWFLKDPETHSYTLEP---------ELLEYLAQYIKENGPFDGIGGFSQGAGLAGY 116
W+ + E S++ E + + ++I+ENGPFDG+ GFSQGA +
Sbjct: 58 ---RAWWFSNNEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHL 114
Query: 117 LSTDLWSILPLNKEEQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELDSLVT 176
L L + + P +FA++FSGF + + + ++H+ G+ D +V
Sbjct: 115 LIAK----AQLGEIKLPGIRFAIFFSGFLSLSSKHDSLTLLRIKEFPSMHVFGDADEIVA 170
Query: 177 EERSMKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWLHAQADENL 225
+S K+ + D + H GGH +P+ I ++ Q D +
Sbjct: 171 RPKSEKMADMFDVEP-LRIAHDGGHVVPSMSKHKEKIAGFMREQLDRKI 218
>Hs18201880 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 227
Score = 83.6 bits (205), Expect = 3e-16
Identities = 69/230 (30%), Positives = 105/230 (45%), Gaps = 23/230 (10%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFND-SEVDLRDSEK 64
+VL L G+ QS+ FR KT +RK L+ + EL+ GP+ + + + D
Sbjct: 8 RVLCLAGFRQSERGFREKTGALRKALRGRA-ELVCLSGPHPVPDPPGPEGARSDFGSCPP 66
Query: 65 STGMYGWFLKDPETHSYTL--EP-------ELLEYLAQYIKENGPFDGIGGFSQGAGLAG 115
GW+ + E ++ EP E L +AQ + GPFDG+ GFSQGA LA
Sbjct: 67 EEQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAALAA 126
Query: 116 YLSTDLWSILPLNKEEQPPF---KFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELD 172
+ L + P F +F + SGF F+ P+ L +LH+ G+ D
Sbjct: 127 L-------VCALGQAGDPRFPLPRFILLVSGFCPRGIGFKESILQRPLSLPSLHVFGDTD 179
Query: 173 SLVTEERSMKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWLHAQAD 222
++ + S++L P T+ HSGGH+IP P L +L A+
Sbjct: 180 KVIPSQESVQLASQF-PGAITLT-HSGGHFIPAAAPQRQAYLKFLDQFAE 227
>7300631 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 279
Score = 71.6 bits (174), Expect = 1e-12
Identities = 69/253 (27%), Positives = 105/253 (41%), Gaps = 27/253 (10%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFNDSEVDLRDS--- 62
+VL LHGY Q+ F+ K RK K E ++ P+ + + V + S
Sbjct: 32 RVLCLHGYRQNGEAFKNKLGSFRKFANKYA-EFVFITAPHVAKALESAAEPVPEQRSWWA 90
Query: 63 EKSTGMYGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAGYL----S 118
K G + K + E L + + + GPF G+ GFSQGA G +
Sbjct: 91 NKDDGSFKGTNKGGPAFGFQ---ESLRCVEEAWRTQGPFQGLLGFSQGACFVGLICGLAK 147
Query: 119 TDLWSILPLNKEEQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELDSLVTEE 178
L SI P +FA+ SGF + YE I + TLHI G+ D ++ +E
Sbjct: 148 KKLTSIRP---------EFAVLASGFLSGSLVHMSAYE-EAISIPTLHIYGQTDEIIPKE 197
Query: 179 RSMKLFEACDPSTRTMVKHSGGHYIPNT----KPFINMILSWLHAQADENLRELSGSEES 234
S L A +++HSGGHY P T + FIN L + + SG+
Sbjct: 198 MSESL--AARFKNAEVLEHSGGHYFPATAQQKQTFINFFQDRLQEYLEHEELQQSGNASF 255
Query: 235 AMKNTVNNSDPQL 247
+++D ++
Sbjct: 256 VDSGAEDDNDAEV 268
>At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 229
Score = 71.2 bits (173), Expect = 1e-12
Identities = 69/239 (28%), Positives = 103/239 (42%), Gaps = 41/239 (17%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLH-------------SKDF 52
++L LHG+ Q+ F+ +T + K+L K EL++ P++L +K F
Sbjct: 3 RILCLHGFRQNASSFKGRTGSLAKKL-KNIAELVFIDAPHELQFIYQTATPPSGVCNKKF 61
Query: 53 NDSEVDLRDSEKSTGMYGWFLK----DPETHSYTLE--PELLEYLAQYIKENGPFDGIGG 106
D TG W + DP + E + L YL +E GPFDGI G
Sbjct: 62 AWLVSSDFDKPSETG---WTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 118
Query: 107 FSQGAGLAGYLSTDLWSILPLNKEEQ----PPFKFAMYFSGFKFEPEQFQAPYETHPIQL 162
FSQGA +A + K+EQ F+F + SG F P E I+
Sbjct: 119 FSQGAAMAAAV---------CGKQEQLVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKC 167
Query: 163 KTLHIVGE---LDSLVTEERSMKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWLH 218
+LHI G D + + S L + T+V+H GH IP P+I+ I ++L+
Sbjct: 168 PSLHIFGSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIPTKSPYIDEIKAFLY 226
>At5g65400 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 234
Score = 65.5 bits (158), Expect = 7e-11
Identities = 64/236 (27%), Positives = 94/236 (39%), Gaps = 19/236 (8%)
Query: 6 KVLMLHGYSQSDVIFRAKTAGMRKQLQKKGYELIYPCGPYKLHSKDFNDSEVDLRDSEKS 65
++L LHG+ S I +A G + +L + P+ K + D
Sbjct: 13 RILCLHGFRTSGRILQAGI-GKWPDTILRDLDLDFLDAPFPATGKSDVERFFD------- 64
Query: 66 TGMYGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAGYLSTDLWSIL 125
Y W+ + Y E L Y+ Y+ +NGPFDG+ GFSQGA L +
Sbjct: 65 PPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGAFLTAAIPGMQEQGS 124
Query: 126 PLNKEEQPPFKFAMYFSGFKFEPEQFQAPYE-----THPIQLKTLHIVGELDSLVTEERS 180
L K P KF + SG K F P + P++ +LH +GE D L E
Sbjct: 125 ALTK--VPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFIGERDFLKIEGEV 182
Query: 181 MKLFEACDPSTRTMVKHSGGHYIPNTKPFINMILSWLHAQADENLRELSGSEESAM 236
+ + +P ++ HSGGH IP + + L + SGS S M
Sbjct: 183 L-VESFVEP---VVIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSDESGSVRSLM 234
>At4g24380 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 208
Score = 58.5 bits (140), Expect = 9e-09
Identities = 47/172 (27%), Positives = 77/172 (44%), Gaps = 7/172 (4%)
Query: 69 YGWFLKDPETHSYTLEPELLEYLAQYIKENGPFDGIGGFSQGAGLAGYLSTDLWSILPLN 128
Y WF + E YT + LEYL + + GPFDG+ GFSQGA L+G L +
Sbjct: 42 YEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPG--LQAKGIA 99
Query: 129 KEEQPPFKFAMYFSGFKFEPEQFQAPYETHPIQLKTLHIVGELDSLVTEERSMKLFEACD 188
++ P KF + G K + + + ++ +LH +GE D L + +L E+
Sbjct: 100 FQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFL--KPYGTQLIESY- 156
Query: 189 PSTRTMVKHSGGHYIPN-TKPFINMILSWLHAQADENLRELSGSEESAMKNT 239
+V H GH +P + + + +++ + E EE+ K T
Sbjct: 157 -KNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDKNGEENTSKPT 207
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 17,371,392
Number of Sequences: 60738
Number of extensions: 797257
Number of successful extensions: 1995
Number of sequences better than 1.0e-05: 11
Number of HSP's better than 0.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 1967
Number of HSP's gapped (non-prelim): 11
length of query: 261
length of database: 30,389,216
effective HSP length: 104
effective length of query: 157
effective length of database: 24,072,464
effective search space: 3779376848
effective search space used: 3779376848
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)