ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1735 suspect: HSP O KOG4597 Posttranslational modification, protein turnover, chaperones Serine proteinase inhibitor (KU family) with thrombospondin repeats
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1735 617508 613225 -1428
(1428 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
7301679 [O] KOG4597 Serine proteinase inhibitor (KU family) with... 74 2e-12
SPBC1711.05 [Y] KOG2992 Nucleolar GTPase/ATPase p130 65 1e-09
YKR092c [Y] KOG2992 Nucleolar GTPase/ATPase p130 61 1e-08
SPCC737.08 [R] KOG1808 AAA ATPase containing von Willebrand fact... 60 3e-08
CE07007 [S] KOG4735 Extracellular protein with conserved cysteines 54 2e-06
Hs16445033 [S] KOG3854 SPRT-like metalloprotease 52 9e-06
>7301679 [O] KOG4597 Serine proteinase inhibitor (KU family) with
thrombospondin repeats
Length = 2230
Score = 73.6 bits (179), Expect = 2e-12
Identities = 108/510 (21%), Positives = 188/510 (36%), Gaps = 44/510 (8%)
Query: 115 IMEDNMKPMDQTDSKGVRVS-DGIQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNAS 173
+ D+ P D+ +S G G + DS + +E S D+E + T + S
Sbjct: 715 MQSDSPTPYDEFESTGTTFEGSGYDSESTTDSGISTEGS----GDDEETSEAST---DLS 767
Query: 174 VSILQGQTSEYSEGDSINLSERRDSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSENIAS 233
S G TS S + S D++ SE+ S D D +D S +++S
Sbjct: 768 SSTDSGSTSSDSTSSDSSSSISSDAT-SEAPASSVSDSSDSTDASTETTGVSDDSTDVSS 826
Query: 234 TPRL---KQTFMQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFLSDNNSEDNIEHEEDV 290
+ + T + + G+ TP ++ A +D+ SDN+S + E
Sbjct: 827 STEASASESTDVSGASDSTGSTNASDSTPESSTEASSSTDDSTDSSDNSSNVSESSTEAS 886
Query: 291 MLSDYQSSDIRESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASNYDESK 350
S S+D + G+ S T N D+ ++ S S+D T+D ES
Sbjct: 887 SSSVSDSNDSSDGSTDGV-SSTTENSSDSTSDAT-SDSTASSDSTDSTSDQTTETTPESS 944
Query: 351 TNGKSEEELPSDQESDLMQDQSSEIEDFDDGEISQQISSLSDEFSIANQAIAQLNSHATX 410
T+ L + +D S E DG + S+ S+ +++ + A
Sbjct: 945 TDSTESSTLDASSTTDASSTSESSSESSTDGSSTTSNSASSETTGLSSDGSTTDATTAAS 1004
Query: 411 XXXXXXXXXXXXIYSEESYNKXXXXXXXXXXXXKVINKEYQTSSEAPEII------TDEE 464
++ S N I+ E S+E+ + I T+
Sbjct: 1005 DNTDITTDGSTDESTDGSSNASTEGSTEGASEDTTISTESSGSTESTDAIASDGSTTEGS 1064
Query: 465 DITQLSATNTHELRSDGI----GELGEYSYQLDTNNNGYGLITDNDHSLILKTLHERSVP 520
+ LS++ + ++ SD E S D++++ G TD + T S
Sbjct: 1065 TVEDLSSSTSSDVTSDSTITDSSPSTEVSGSTDSSSSTDGSSTDASSTEASSTDVTESTD 1124
Query: 521 KNVEYSENNVNTTGSGNSEE-----LSETSVQDIDFASNAIDLPQTT---LSKTEKEVFA 572
V S +TT SG +EE +E++ + ++ + DL TT S ++K+ +
Sbjct: 1125 STV--SGGTSDTTESGPTEESTTEGSTESTTEGSTDSTQSTDLDSTTSDIWSTSDKDDES 1182
Query: 573 EPKEPCMVTKEATSVERQERNVGQSEPRYC 602
E P E T +S+PR C
Sbjct: 1183 ESSTPYSFDSEVT----------KSKPRKC 1202
>SPBC1711.05 [Y] KOG2992 Nucleolar GTPase/ATPase p130
Length = 451
Score = 64.7 bits (156), Expect = 1e-09
Identities = 86/395 (21%), Positives = 154/395 (38%), Gaps = 41/395 (10%)
Query: 149 SEDSIVILSDNEGSENIHTMNKNASVSILQGQTSEYSEGDSINLSERRDSSHSESIIEQA 208
++DS I E H+ ++ AS S S+ S +S + SE DSS S S E
Sbjct: 80 NDDSQKISKKGAPPEKAHSSSE-ASGSGSSSDESDSSSSESESSSEDNDSSSSSSDSESE 138
Query: 209 EPSDDEDDDLFNEYSDNDLSENIASTPRLKQTFMQAYAQKPGNKTTDSVTPRDTSMAGVE 268
S+D D + S+++ S + S + ++ + N ++ S + ++ + +
Sbjct: 139 SSSEDSDSSSSSSDSESE-SSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSED 197
Query: 269 LEDNDFLSDNNSEDNIEHEEDVMLSDYQSSDIRESKPSGIFSHQTLNDKDNLEESEGSPE 328
+ + SD+ SE + E + S SS ES S + +D ++ SE S
Sbjct: 198 SDSSSSSSDSESESSSEGSDS---SSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDS 254
Query: 329 YYSTDDEKLTNDALASNYDESKTNGKSEEELPSDQESDLMQDQSSEIEDFDDGEISQQIS 388
S+ D + +++ + + D S + SE++ SD SS+ E E S S
Sbjct: 255 SSSSSDSE--SESSSKDSDSSSNSSDSEDDSSSD---------SSDSESESSSEDSDSTS 303
Query: 389 SLSDEFSIANQAIAQLNSHATXXXXXXXXXXXXXIYSEESYNKXXXXXXXXXXXXKVINK 448
S SD S ++ N+ T SEES K+ +K
Sbjct: 304 SSSDSDSSSSSEDGNSNTDTTTSGEVSAQSSTNSTSSEES------TSVKDEDSSKIHDK 357
Query: 449 EYQTSSEAPEIITDEEDITQLSATNTHELRSDGIGELGEYSY---QLDTNN----NGYGL 501
+ E D+E T ++ T + +G+ ++ + L N+ + YG
Sbjct: 358 SLKRKHE------DDESSTSTKSSRTTKTPFTRVGDPSQWDFASPALRDNSFNFEDDYGT 411
Query: 502 ITDNDHSLILKT----LHERSVPKNVEYSENNVNT 532
+ + D LI+ E++ K Y +NT
Sbjct: 412 LANRD--LIVTRGKGFRQEKNKKKRGSYRGGRINT 444
Score = 60.1 bits (144), Expect = 2e-08
Identities = 62/272 (22%), Positives = 115/272 (41%), Gaps = 19/272 (6%)
Query: 122 PMDQTDSKGVRVSDGIQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNASVSILQGQT 181
P ++ S G + S ESE S S++ S + + +++ S S +
Sbjct: 92 PPEKAHSSSEASGSGSSSDESDSSSSESESS----SEDNDSSSSSSDSESESSSEDSDSS 147
Query: 182 SEYSEGDSINLSERRDSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSENIASTPRLKQTF 241
S S+ +S + SE DSS S S E S+D D + S+++ S + + +
Sbjct: 148 SSSSDSESESSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDS----SSS 203
Query: 242 MQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFL---SDNNSEDNIEHEEDVMLSDYQSS 298
+ ++ +DS + +S + EDND SD+ SE + E + D S S
Sbjct: 204 SSDSESESSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSE-DSDSSSSSSDSE 262
Query: 299 DIRESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASNYDESKTNGKSEEE 358
SK S S+ + ++ D+ +S S S++D T+ + S+ S +G S +
Sbjct: 263 SESSSKDSDSSSNSSDSEDDSSSDSSDSESESSSEDSDSTSSSSDSDSSSSSEDGNSNTD 322
Query: 359 LPSDQE-------SDLMQDQSSEIEDFDDGEI 383
+ E + ++S+ ++D D +I
Sbjct: 323 TTTSGEVSAQSSTNSTSSEESTSVKDEDSSKI 354
Score = 58.5 bits (140), Expect = 7e-08
Identities = 63/283 (22%), Positives = 114/283 (40%), Gaps = 16/283 (5%)
Query: 121 KPMDQTDSKGVRVSDGIQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNASVSILQGQ 180
K +++ G S ++S SED+ S ++ + + ++S S +
Sbjct: 95 KAHSSSEASGSGSSSDESDSSSSESESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSE 154
Query: 181 TSEYSEG----------DSINLSERRDSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSEN 230
+ SEG +S + SE DSS S S E S+D D + S+++ S +
Sbjct: 155 SESSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESE-SSS 213
Query: 231 IASTPRLKQTFMQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFLSDNNSEDNIEHEEDV 290
S + ++ + N ++ S + ++ + + + + SD+ SE + + D
Sbjct: 214 EGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDSSSSSSDSESESS-SKDSDS 272
Query: 291 MLSDYQSSDIRESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASN--YDE 348
+ S D S S S + D D+ S S S++D D S +
Sbjct: 273 SSNSSDSEDDSSSDSSDSESESSSEDSDSTSSSSDSDSSSSSEDGNSNTDTTTSGEVSAQ 332
Query: 349 SKTNGKSEEELPS--DQESDLMQDQSSEIEDFDDGEISQQISS 389
S TN S EE S D++S + D+S + + DD + SS
Sbjct: 333 SSTNSTSSEESTSVKDEDSSKIHDKSLKRKHEDDESSTSTKSS 375
Score = 53.9 bits (128), Expect = 2e-06
Identities = 65/278 (23%), Positives = 113/278 (40%), Gaps = 29/278 (10%)
Query: 117 EDNMKPMDQTDSKGVRVSDGIQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNASVSI 176
ED+ +DS+ S+G + S ESE S S++ S + + +++ S S
Sbjct: 142 EDSDSSSSSSDSESESSSEGSDSSSSSSSS-ESESS----SEDNDSSSSSSDSESESSSE 196
Query: 177 LQGQTSEYSEGDSINLSERRDSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSENIASTPR 236
+S S+ +S + SE DSS S S E S+D D + S+++ S + +
Sbjct: 197 DSDSSSSSSDSESESSSEGSDSSSSSSSSESESSSEDNDSSSSSSDSESESSSEDSDSSS 256
Query: 237 LKQTFMQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFLSDNNSED----NIEHEEDVML 292
+ K + +++S D S + + +D S+++SED + + D
Sbjct: 257 SSSDSESESSSKDSDSSSNSSDSEDDSSS----DSSDSESESSSEDSDSTSSSSDSDSSS 312
Query: 293 SDYQSSDIRESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASNY--DESK 350
S + ++ SG S Q+ N SE S D K+ + +L + DES
Sbjct: 313 SSEDGNSNTDTTTSGEVSAQS---STNSTSSEESTSVKDEDSSKIHDKSLKRKHEDDESS 369
Query: 351 TNGKSEEELPS-----------DQESDLMQDQSSEIED 377
T+ KS + D S ++D S ED
Sbjct: 370 TSTKSSRTTKTPFTRVGDPSQWDFASPALRDNSFNFED 407
>YKR092c [Y] KOG2992 Nucleolar GTPase/ATPase p130
Length = 406
Score = 60.8 bits (146), Expect = 1e-08
Identities = 61/278 (21%), Positives = 108/278 (37%), Gaps = 13/278 (4%)
Query: 137 IQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNASVSILQGQTSEYSEGDSINLSERR 196
I+ K + S S S S + S + + ++S S + DS + S
Sbjct: 21 IEEKSSSSSSSSSSSSSSSSSSSSSSSSSGESSSSSSSSSSSSSSDSSDSSDSESSSSSS 80
Query: 197 DSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSENIASTPRLKQTFMQAYAQKPGN---KT 253
SS S S +E S + D S + S + +S+ + + A++ N K
Sbjct: 81 SSSSSSSSSSDSESSSESDSSSSGSSSSSSSSSDESSSESESEDETKKRARESDNEDAKE 140
Query: 254 TDSVTPRDTSMAGVELEDNDFLSDNNSEDNIEHEEDVMLSDYQSSDIRESKPSGIFSHQT 313
T S + E + S + SE E + D S SSD S S +
Sbjct: 141 TKKAKTEPESSSSSESSSSGSSSSSESESGSESDSDSSSSSSSSSDSESDSESDSQSSSS 200
Query: 314 LNDKDNLEESEGSPEYYSTDDEKLT--------NDALASNYDESKTNGKSEEELPSDQES 365
+ D+ +S+ S S+D + + +D+ + + +S ++G S+ SD S
Sbjct: 201 SSSSDSSSDSDSSSSDSSSDSDSSSSSSSSSSDSDSDSDSSSDSDSSGSSDSSSSSDSSS 260
Query: 366 DLMQDQSSEIEDFDDGEISQQISSLSDEFSIANQAIAQ 403
D + SS+ D D S S L + + A+++ A+
Sbjct: 261 D--ESTSSDSSDSDSDSDSGSSSELETKEATADESKAE 296
>SPCC737.08 [R] KOG1808 AAA ATPase containing von Willebrand factor type A (vWA)
domain
Length = 4717
Score = 59.7 bits (143), Expect = 3e-08
Identities = 64/249 (25%), Positives = 109/249 (43%), Gaps = 31/249 (12%)
Query: 156 LSDNEGSENI-HTMNKNASVSILQGQT-----SEYSEGDSINLSERRDSSHSESIIEQAE 209
L G+E+I +T+N++ + L + S+ E ++ L + +S++ + E
Sbjct: 3914 LGSGVGAEDITNTLNEDDDLEELANEEDTANQSDLDESEARELESDMNGVTKDSVVSENE 3973
Query: 210 PSDDEDD--DLFNEYSD------NDLSENIASTPR---LKQTFMQAYAQKPGNKTTDSVT 258
SD E++ DL E +D N L+E + P L +T ++ Q N +D V+
Sbjct: 3974 NSDSEEENQDLDEEVNDIPEDLSNSLNEKLWDEPNEEDLLETEQKSNEQSAANNESDLVS 4033
Query: 259 PRDTSMAGVELEDNDFLSDNNSEDNIEHEEDVMLSDYQSSDIRESK---PSGIFSHQTLN 315
D + A LED D ED E +DV + D DI+E+ P H L
Sbjct: 4034 KEDDNKA---LEDKD---RQEKEDEEEMSDDVGIDDEIQPDIQENNSQPPPENEDHLDLP 4087
Query: 316 DKDNLEESEGSPEYYSTDDEKLTNDALASNYDESKTNGKSEEELPSDQESDLMQDQSSEI 375
+ L+E EG S D + D A+ DE+K +E++ P D +++ ++
Sbjct: 4088 EDLKLDEKEGD---VSKDSDLEDMDMEAA--DENKEEADAEKDEPMQDFEDPLEENNTLD 4142
Query: 376 EDFDDGEIS 384
ED + S
Sbjct: 4143 EDIQQDDFS 4151
>CE07007 [S] KOG4735 Extracellular protein with conserved cysteines
Length = 727
Score = 53.9 bits (128), Expect = 2e-06
Identities = 76/286 (26%), Positives = 120/286 (41%), Gaps = 43/286 (15%)
Query: 127 DSKGVR-VSDGIQMKDEADSCLESEDSIVILSDNEGSENIHTMNKNAS----------VS 175
DSK VSD Q D+ + S S S N GSEN + +A VS
Sbjct: 43 DSKSASPVSDKQQYSDQQKEVVNSNSSNTE-SKNAGSENPGSSETSADQTANEPKPDKVS 101
Query: 176 ILQGQTSEYSEGDSINLSERRDSSHS--ESIIEQAEPSDDEDDDLFNEYSDNDLSENIAS 233
++E E + I D+S +S + + P+ +ED+ + E + EN +
Sbjct: 102 DDNAGSAETIESNGIEKIPSNDTSSPIFDSAVVPSGPAQEEDEAVEEEKKVEEEHENHGN 161
Query: 234 TPRLKQTFMQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFLSDNN-SEDNIEHEEDVML 292
+ ++ NK+ D+VT T ++ + E ND D + S + + E+
Sbjct: 162 SEDAEE-----------NKSGDAVTTT-TELSMEDREGNDDPKDESESGTTVSNNEETTT 209
Query: 293 SDYQSSDIRESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASNYDESKTN 352
++ + +D SK + S Q D D++E+ EG E +T DE T E KTN
Sbjct: 210 TENKVADEAPSKEAE--SIQASQDSDHVEK-EGESETTTTSDESATT-------TEKKTN 259
Query: 353 G-----KSEEELPSDQESDLMQDQSSEIEDFDDGEISQQISSLSDE 393
G KSE E + S + +SE D I +Q+ S +DE
Sbjct: 260 GAELVEKSETEEKNGDSSTKSPEVTSE-SSVDTTTIEEQLKSNNDE 304
>Hs16445033 [S] KOG3854 SPRT-like metalloprotease
Length = 691
Score = 51.6 bits (122), Expect = 9e-06
Identities = 67/298 (22%), Positives = 127/298 (42%), Gaps = 60/298 (20%)
Query: 137 IQMKDEADSCLESEDSI----VILSDNEGSEN----IHTMNKNASVSILQGQTSEYSEGD 188
I DE+ C + +I +++SD++ ++ + + N+ S S+ SE
Sbjct: 100 INSDDESPECCHVKPAIQEPPIVISDDDNDDDNGNDLEVPDDNSDDSEAPDDNSDDSEAP 159
Query: 189 SINLSERR-------DSSHSESIIEQAEPSDDEDDDLFNEYSDNDLSENIASTPRLKQTF 241
N + DS + + ++ DD DD ++ SD+ +N +
Sbjct: 160 DDNSDDSEAPDDNSDDSEAPDDNSDDSDVPDDNSDDSSDDNSDDSSDDNSDDS------- 212
Query: 242 MQAYAQKPGNKTTDSVTPRDTSMAGVELEDNDFLSDNNSEDNIEHEEDVMLSDYQSSDIR 301
P +K+ DS P D+S +D+D D S D+ E +D SSD
Sbjct: 213 -----DVPDDKSDDSDVPDDSS------DDSDVPDD--SSDDSEAPDD-------SSDDS 252
Query: 302 ESKPSGIFSHQTLNDKDNLEESEGSPEYYSTDDEKLTNDALASNYDESKTNGKSEEELPS 361
E+ S + D+ ++SE + S+DD + ++D +S+ E+ + + E P
Sbjct: 253 EAPDDS--SDDSEAPDDSSDDSEAPDD--SSDDSEASDD--SSDDSEASDDSSDDSEAPD 306
Query: 362 DQ--ESDLMQDQSSEI----EDFDDGEISQQISSLSDEFSIAN------QAIAQLNSH 407
D+ +SD+ +D+S + ++ DD E+ L +E IA+ +A A ++ H
Sbjct: 307 DKSDDSDVPEDKSDDSDVPDDNSDDLEVPVPAEDLCNEGQIASDEEELVEAAAAVSQH 364
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.304 0.124 0.329
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,196,304
Number of Sequences: 60738
Number of extensions: 3263520
Number of successful extensions: 11209
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 11069
Number of HSP's gapped (non-prelim): 37
length of query: 1428
length of database: 30,389,216
effective HSP length: 119
effective length of query: 1309
effective length of database: 23,161,394
effective search space: 30318264746
effective search space used: 30318264746
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (21.9 bits)