ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1828 good I KOG1511 Lipid transport and metabolism Mevalonate kinase MVK/ERG12
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1828 652705 651449 -419
(419 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YMR208w [I] KOG1511 Mevalonate kinase MVK/ERG12 340 3e-93
Hs4557769 [I] KOG1511 Mevalonate kinase MVK/ERG12 149 6e-36
SPAC13G6.11c [I] KOG1511 Mevalonate kinase MVK/ERG12 145 1e-34
At5g27450 [I] KOG1511 Mevalonate kinase MVK/ERG12 130 4e-30
7303424 [I] KOG1511 Mevalonate kinase MVK/ERG12 108 1e-23
CE29110 [I] KOG1511 Mevalonate kinase MVK/ERG12 69 2e-11
>YMR208w [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 443
Score = 340 bits (871), Expect = 3e-93
Identities = 194/441 (43%), Positives = 258/441 (57%), Gaps = 32/441 (7%)
Query: 10 PFIVSAPGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIEQSKDGDGVELDFPDINYDSKW 69
PF+ SAPGKVIIFGEHSAV+NK A+AASVS+LRTYLLI +S D +ELDFPDI+++ KW
Sbjct: 4 PFLTSAPGKVIIFGEHSAVYNKPAVAASVSALRTYLLISESSAPDTIELDFPDISFNHKW 63
Query: 70 STEELKTIDSEVVKQAAGLKHXXXXXXXXXXXXX------XXXXDXXXXXXXXXXXXXXX 123
S + I + V K +
Sbjct: 64 SINDFNAITEDQVNSQKLAKAQQATDGLSQELVSLLDPLLAQLSESFHYHAAFCFLYMFV 123
Query: 124 XXCPNVKGLKFTVKSTLPXXXXXXXXXXXXVCLALAMARMGGHISCD--KSISQEEKEFI 181
CP+ K +KF++KSTLP V LALAMA +GG I + + +S+ +K +
Sbjct: 124 CLCPHAKNIKFSLKSTLPIGAGLGSSASISVSLALAMAYLGGLIGSNDLEKLSENDKHIV 183
Query: 182 NHWAFIGECCIHGTPSGIDNAVATYGNAVKFKRQEDGST----KFDQISNFPQLPMVLTN 237
N WAFIGE CIHGTPSGIDNAVATYGNA+ F++ T F + +FP +PM+LT
Sbjct: 184 NQWAFIGEKCIHGTPSGIDNAVATYGNALLFEKDSHNGTINTNNFKFLDDFPAIPMILTY 243
Query: 238 TRIKRSTKILVSGVGDLVNRK-PLITEPILEAMNQVALRGSELLSDISK----------- 285
TRI RSTK LV+ V LV K P + +PIL+AM + AL+G E+++ +SK
Sbjct: 244 TRIPRSTKDLVARVRVLVTEKFPEVMKPILDAMGECALQGLEIMTKLSKCKGTDDEAVET 303
Query: 286 -DIHYDELLELIRINHGLLVAIGVSHPGLEVIKQLSDELKIGQTKLTXXXXXXCAFTLLN 344
+ Y++LLELIRINHGLLV+IGVSHPGLE+IK LSD+L+IG TKLT C+ TLL
Sbjct: 304 NNELYEQLLELIRINHGLLVSIGVSHPGLELIKNLSDDLRIGSTKLTGAGGGGCSLTLLR 363
Query: 345 KDVDHSSVRKFHEKLHSNYGYQIFETGLGGVGCCFVDLKNEQYE-SLKHLFID-----SA 398
+D+ + F +KL ++ Y+ FET LGG GCC + KN + +K L +
Sbjct: 364 RDITQEQIDSFKKKLQDDFSYETFETDLGGTGCCLLSAKNLNKDLKIKSLVFQLFENKTT 423
Query: 399 TVQEVSLALLPSPDGSLTWTA 419
T Q++ LLP + +L WT+
Sbjct: 424 TKQQIDDLLLPG-NTNLPWTS 443
>Hs4557769 [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 396
Score = 149 bits (377), Expect = 6e-36
Identities = 109/379 (28%), Positives = 178/379 (46%), Gaps = 23/379 (6%)
Query: 12 IVSAPGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIEQSKDGDGVELDFPDINYDSKWST 71
+VSAPGKVI+ GEH+ V K A+A S++ LRT+L ++ +G V+L P+I W
Sbjct: 7 LVSAPGKVILHGEHAVVHGKVALAVSLN-LRTFLRLQPHSNGK-VDLSLPNIGIKRAWDV 64
Query: 72 EELKTIDSEVVKQA----AGLKHXXXXXXXXXXXXXXXXXDXXXXXXXXXXXXXXXXXCP 127
L+++D+ ++Q + +
Sbjct: 65 ARLQSLDTSFLEQGDVTTPTSEQVEKLKEVAGLPDDCAVTERLAVLAFLYLYLSICRKQR 124
Query: 128 NVKGLKFTVKSTLPXXXXXXXXXXXXVCLALAMARMGGHI-------SCDKSISQEEKEF 180
+ L V S LP VCLA A+ + I C ++E+ E
Sbjct: 125 ALPSLDIVVWSELPPGAGLGSSAAYSVCLAAALLTVCEEIPNPLKDGDCVNRWTKEDLEL 184
Query: 181 INHWAFIGECCIHGTPSGIDNAVATYGNAVKFKRQEDGSTKFDQISNFPQLPMVLTNTRI 240
IN WAF GE IHG PSG+DNAV+T+G A+++ + K + P L ++LTNT++
Sbjct: 185 INKWAFQGERMIHGNPSGVDNAVSTWGGALRYHQ-----GKISSLKRSPALQILLTNTKV 239
Query: 241 KRSTKILVSGVGDLVNRKPLITEPILEAMNQVALRGSELLSDISK---DIHYDELLELIR 297
R+T+ LV+GV + + + P I P+L +++ ++L +L ++ + Y L ELI
Sbjct: 240 PRNTRALVAGVRNRLLKFPEIVAPLLTSIDAISLECERVLGEMGEAPAPEQYLVLEELID 299
Query: 298 INHGLLVAIGVSHPGLEVIKQLSDELKIGQTKLTXXXXXXCAFTLLNKDVDHSSVRKFHE 357
+N L A+GV H L+ + Q++ + +KLT C TLL ++ V +
Sbjct: 300 MNQHHLNALGVGHASLDQLCQVTRARGL-HSKLTGAGGGGCGITLLKPGLEQPEVEATKQ 358
Query: 358 KLHSNYGYQIFETGLGGVG 376
L ++ G+ ET +G G
Sbjct: 359 AL-TSCGFDCLETSIGAPG 376
>SPAC13G6.11c [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 404
Score = 145 bits (366), Expect = 1e-34
Identities = 115/390 (29%), Positives = 183/390 (46%), Gaps = 21/390 (5%)
Query: 12 IVSAPGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIEQSKDGDGVELDFPDINYDSKWST 71
IVS+PGK I+FGEH+ V+ A+AA+VS LR+Y ++ + + + + + DI + +W+
Sbjct: 6 IVSSPGKTILFGEHAVVYGATALAAAVS-LRSYCKLQTTNNNE-IVIVMSDIGTERRWNL 63
Query: 72 EEL--KTIDSEVVKQAAGLKHXXXXXXXXXXXXXXXXXDXXXXXXXXXXXXXXXXXCPNV 129
+ L + + E V+ A + P+
Sbjct: 64 QSLPWQHVTVENVQHPASSPNLDLLQGLGELLKNEENG-LIHSAMLCTLYLFTSLSSPS- 121
Query: 130 KGLKFTVKSTLPXXXXXXXXXXXXVCLALAMARMGGHISCDKSISQEEKE---FINHWAF 186
+G T+ S +P V +A ++ G+I S S + + I W+F
Sbjct: 122 QGCTLTISSQVPLGAGLGSSATISVVVATSLLLAFGNIEPPSSNSLQNNKALALIEAWSF 181
Query: 187 IGECCIHGTPSGIDNAVATYGNAVKFKRQEDGSTKFDQ-ISNFPQLPMVLTNTRIKRSTK 245
+GECCIHGTPSGIDNAVAT G + F++ + + + L +++T+T+ +STK
Sbjct: 182 LGECCIHGTPSGIDNAVATNGGLIAFRKATAHQSAMKEFLKPKDTLSVMITDTKQPKSTK 241
Query: 246 ILVSGVGDLVNRKPLITEPILEAMNQVALRG-SELLSDISKDIHYDELLELIRINHGLLV 304
LV GV +L R P + + I++A++ ++ L S+ K+ +L E I +N LL
Sbjct: 242 KLVQGVFELKERLPTVIDSIIDAIDGISKSAVLALTSESDKNSSAKKLGEFIVLNQKLLE 301
Query: 305 AIGVSHPGLEVIKQLSDELKIGQTKLTXXXXXXCAFTLLNKDVDHSSVRKFHEKL--HSN 362
+GVSH ++ + Q + IG TKLT C TLL + + E L H N
Sbjct: 302 CLGVSHYSIDRVLQATK--SIGWTKLTGAGGGGCTITLLTPECKEEEFKLCKESLLAHKN 359
Query: 363 YGYQIFETGLGGVGCCFV---DLKNEQYES 389
I++ LGG G V D QYES
Sbjct: 360 ---SIYDVQLGGPGVSVVTDSDSFFPQYES 386
>At5g27450 [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 378
Score = 130 bits (327), Expect = 4e-30
Identities = 109/380 (28%), Positives = 170/380 (44%), Gaps = 31/380 (8%)
Query: 15 APGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIE----QSKDGDGVELDFPDINYDSKWS 70
APGK+I+ GEH+ V A+AA++ L TY+ + +++ D + L DI+ + WS
Sbjct: 7 APGKIILAGEHAVVHGSTAVAAAID-LYTYVTLRFPLPSAENNDRLTLQLKDISLEFSWS 65
Query: 71 TEELKTI---DSEVVKQA--AGLKHXXXXXXXXXXXXXXXXXDXXXXXXXXXXXXXXXXX 125
+K DS + ++ A +
Sbjct: 66 LARIKEAIPYDSSTLCRSTPASCSEETLKSIAVLVEEQNLPKEKMWLSSGISTFLWLYTR 125
Query: 126 CPNVKGLKFTVKSTLPXXXXXXXXXXXXVCLALAMARMGGHISCD------KSISQEEKE 179
+ S LP +C+AL A + IS S+ + E
Sbjct: 126 IIGFNPATVVINSELPYGSGLGSSAA--LCVALTAALLASSISEKTRGNGWSSLDETNLE 183
Query: 180 FINHWAFIGECCIHGTPSGIDNAVATYGNAVKFKRQEDGSTKFDQISNFPQLPMVLTNTR 239
+N WAF GE IHG PSGIDN V+ YGN +KF E + SN P L M++TNTR
Sbjct: 184 LLNKWAFEGEKIIHGKPSGIDNTVSAYGNMIKFCSGEITRLQ----SNMP-LRMLITNTR 238
Query: 240 IKRSTKILVSGVGDLVNRKPLITEPILEAMNQVALRGSELL---SDISKDIHYDELLELI 296
+ R+TK LVSGV R P + + A++ ++ + ++ + S + + EL+
Sbjct: 239 VGRNTKALVSGVSQRAVRHPDAMKSVFNAVDSISKELAAIIQSKDETSVTEKEERIKELM 298
Query: 297 RINHGLLVAIGVSHPGLEVIKQLSDELKIGQTKLTXXXXXXCAFTLLNKDVDHSSVRKFH 356
+N GLL+++GVSH +E + + + K+ +KLT C TLL + V K
Sbjct: 299 EMNQGLLLSMGVSHSSIEAVILTTVKHKL-VSKLTGAGGGGCVLTLLPTG---TVVDKVV 354
Query: 357 EKLHSNYGYQIFETGLGGVG 376
E+L S+ G+Q F +GG G
Sbjct: 355 EELESS-GFQCFTALIGGNG 373
>7303424 [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 390
Score = 108 bits (271), Expect = 1e-23
Identities = 106/398 (26%), Positives = 163/398 (40%), Gaps = 51/398 (12%)
Query: 11 FIVSAPGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIEQSKDGD-----------GVELD 59
F V +PGKVI+ GEH+ V+++ A+AA V L T L Q +D +E+
Sbjct: 4 FQVHSPGKVILHGEHAVVYHRPALAAVVG-LGTTLRFRQLEDSQVASFQLEALNCTLEIQ 62
Query: 60 FPDIN---------YDSKWSTEELKTIDSEVVKQA------AGLKHXXXXXXXXXXXXXX 104
P+ N Y S + L+ + +EV KQ A +H
Sbjct: 63 LPEFNGFLAEFRSKYPSDSTANLLEAVRAEVSKQLERSGGNAPKQHTQRAFISIYYLLAG 122
Query: 105 XXXDXXXXXXXXXXXXXXXXXCPNVKGLKFTVKSTLPXXXXXXXXXXXXVCLALAMARMG 164
G + V S L LA +
Sbjct: 123 AVLSAPGDKKLTLQT-----------GFQVQVDSQLTVGAGLGSSASFGAALATTFLILA 171
Query: 165 GHISCDKSISQEEKEFINHWAFIGECCIHGTPSGIDNAVATYGNAVKFKRQEDGSTKFDQ 224
GH D +S+ + I+ WA+ E HGTPSG+DN V TYG +++ + + F
Sbjct: 172 GHFDRDSYLSEANQALISSWAYESERVNHGTPSGLDNTVCTYGGMLRYVKGQG----FQS 227
Query: 225 ISNFPQLPMVLTNTRIKRSTKILVSGVGDLVNRKPLITEPILEAMNQVALRGSELLSDIS 284
+ L ++L ++R+ RST +V+ V L + P + E I +A ++ L
Sbjct: 228 LKIQKPLNILLVDSRVSRSTADIVAKVRHLGDAFPQLIEAIWQACEELVAAAVPLYESFG 287
Query: 285 K----DIHYDELLELIRINHGLLVAIGVSHPGLEVIKQLSDELKIG-QTKLTXXXXXXCA 339
+++L L +IN+ LL AIGVSHP LE I ++ K G +KLT
Sbjct: 288 NAQDDSSKFEQLERLFQINNDLLKAIGVSHPKLEQIFTIA--FKRGFFSKLTGAGAGGYV 345
Query: 340 FTLLNKDVDHSSVR-KFHEKLHSNYGYQIFETGLGGVG 376
LL ++ + + V K E+L S G+ + T GG G
Sbjct: 346 IVLLPENYECNEVYWKLKEELES-AGFGVHVTTAGGEG 382
>CE29110 [I] KOG1511 Mevalonate kinase MVK/ERG12
Length = 607
Score = 68.6 bits (166), Expect = 2e-11
Identities = 39/158 (24%), Positives = 76/158 (47%), Gaps = 13/158 (8%)
Query: 132 LKFTVKSTLPXXXXXXXXXXXXVCLALAMARMGGHISCDKSISQEEK---------EFIN 182
+K TV+ LP VC+A ++ + G I ++ E+ + I
Sbjct: 262 VKATVRFKLPSCVGLGSSGAYCVCIATSLLQTAGLIPPPSIVADEQGNLTWEEDHLDIIR 321
Query: 183 HWAFIGECCIHGTPSGIDNAVATYGNAVKFKRQEDGSTKFDQISNFPQLPMVLTNTRIKR 242
WA E IHG SG+D AV T+G FK + + + N P L ++L N++++R
Sbjct: 322 KWATAAESLIHGRASGLDAAVCTFGGVASFK----PGHRIEHLKNLPDLRVILVNSKVER 377
Query: 243 STKILVSGVGDLVNRKPLITEPILEAMNQVALRGSELL 280
+T +V V + + + P + + + +++ ++L +++L
Sbjct: 378 NTARMVQTVKERLKKFPEVVDAMFGSIDAISLDAAKIL 415
Score = 57.4 bits (137), Expect = 4e-08
Identities = 28/62 (45%), Positives = 41/62 (65%), Gaps = 2/62 (3%)
Query: 13 VSAPGKVIIFGEHSAVFNKNAIAASVSSLRTYLLIEQSKDGDGVELDFPDINYDSKWSTE 72
VSAPGK+I+FGEH+ V+ + AIA S+ LRTY+ + S DG + L PD+ + W +
Sbjct: 44 VSAPGKIILFGEHAVVYGRTAIAGSI-DLRTYVSLYTSADG-RIYLSLPDLGVEKTWMLK 101
Query: 73 EL 74
+L
Sbjct: 102 DL 103
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.135 0.392
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,398,808
Number of Sequences: 60738
Number of extensions: 817927
Number of successful extensions: 1855
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1827
Number of HSP's gapped (non-prelim): 14
length of query: 419
length of database: 30,389,216
effective HSP length: 109
effective length of query: 310
effective length of database: 23,768,774
effective search space: 7368319940
effective search space used: 7368319940
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)