ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1957 good R KOG1271 General function prediction only Methyltransferases
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1957 698086 698781 232
(232 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL064w [R] KOG1271 Methyltransferases 271 4e-73
SPBC839.14c [R] KOG1271 Methyltransferases 135 6e-32
7295871 [R] KOG1271 Methyltransferases 130 2e-30
CE27144 [R] KOG1271 Methyltransferases 107 1e-23
At1g66680 [R] KOG1271 Methyltransferases 89 7e-18
CE06739 [E] KOG2352 Predicted spermine/spermidine synthase 54 1e-07
Hs14150112 [E] KOG2352 Predicted spermine/spermidine synthase 53 3e-07
>YIL064w [R] KOG1271 Methyltransferases
Length = 257
Score = 271 bits (694), Expect = 4e-73
Identities = 123/230 (53%), Positives = 171/230 (73%), Gaps = 1/230 (0%)
Query: 1 MEDTTKLNTSKLGTKEYWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLGE 60
M+ T L+TSKLGTK+YWD+ Y+LE +NF NP+DTG+CWF+D+DAE+KM++FL+DN+G
Sbjct: 29 MQGTADLSTSKLGTKKYWDELYALELENFRRNPQDTGDCWFSDSDAEQKMIDFLVDNIGA 88
Query: 61 YNIKEDSSMIDLGTGNGHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEILKTKYSQNDNVT 120
Y I E++S++DLGTGNGH+LF L + F+G+++G+DYSE+SV+ A+ I + N ++
Sbjct: 89 YRISENASVVDLGTGNGHMLFELHQTEFQGKLVGIDYSEESVKLASNIAEATGVDN-FIS 147
Query: 121 FATADIFNEQWAPGKFDVVLDKGTLDAIALSGIKFENNETVVDVYPKVIEKILGENSILL 180
F ADIF+ W PGK+D+VLDKGTLDAI+LSG+K VVDVY V+E+IL ++ I L
Sbjct: 148 FQQADIFSGDWKPGKYDIVLDKGTLDAISLSGMKINGKLDVVDVYAGVVERILKKDGIFL 207
Query: 181 ITSCNFXXXXXXXXXXXXXXXXWKSVNYPIFEFGGVKGTTICSVAFLKSS 230
ITSCNF WK++ YP+F+FGGV+G TICSVAF+K +
Sbjct: 208 ITSCNFTQDELVKIIETDNLKMWKTIKYPVFQFGGVQGATICSVAFVKQN 257
>SPBC839.14c [R] KOG1271 Methyltransferases
Length = 238
Score = 135 bits (339), Expect = 6e-32
Identities = 87/230 (37%), Positives = 126/230 (53%), Gaps = 14/230 (6%)
Query: 7 LNTSKLGTKEYWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLG----EYN 62
L SKLGTK+YWD+ Y E NF E D GE WF + +AEE++V++L D++ E +
Sbjct: 4 LPESKLGTKQYWDNVYEREVSNFTEF-NDEGEVWFGE-EAEERIVQWLEDHISTSFREVS 61
Query: 63 IKEDSSMIDLGTGNGHLLFTLLEE-----GFKGEMIGVDYSEKSVEFAAEILKTKYSQND 117
++DLGTGNGHLLF LLEE +++GVDYSE ++ A I + + +D
Sbjct: 62 EAAPFRVLDLGTGNGHLLFRLLEEEDTLLPSPCQLVGVDYSEAAIVLAKNIARHR-QFSD 120
Query: 118 NVTFATADIFNE-QWAPGKFDVVLDKGTLDAIALSGIKFENNETVVDVYPKVIEKILGEN 176
V F DI + ++ +D++LDKGT DAI+LSG + + + VY + +L N
Sbjct: 121 KVKFQQLDIIKDSKFCSKDWDLILDKGTFDAISLSG-ELLDGRPLNSVYVDRVRGMLSPN 179
Query: 177 SILLITSCNFXXXXXXXXXXXXXXXXWKSVNYPIFEFGGVKGTTICSVAF 226
I LITSCN+ +V P+FEF G G++ +AF
Sbjct: 180 GIFLITSCNWTIQELEERFTKNGFIVHSTVPVPVFEFQGSTGSSTSVIAF 229
>7295871 [R] KOG1271 Methyltransferases
Length = 219
Score = 130 bits (326), Expect = 2e-30
Identities = 82/223 (36%), Positives = 126/223 (55%), Gaps = 12/223 (5%)
Query: 5 TKLNTSKLGTKEYWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLGEYNIK 64
++LN S+LGTKE+W+ Y+ E +N++ + D GE WF D A+ + +++LL+ E K
Sbjct: 3 SELNGSELGTKEFWESSYNREIRNYKSHG-DVGEIWF-DESAQWRTIDWLLNE--EKIDK 58
Query: 65 EDSSMIDLGTGNGHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEILKTKYSQNDNVTFATA 124
E S ++DLG GNG L L EGF G++ GVDYS K+VE A I + ++T+ A
Sbjct: 59 EASRVLDLGCGNGMFLVGLANEGFTGDLTGVDYSPKAVELAQNIAE---DNKLSITYKVA 115
Query: 125 DIFNEQWAPGKFDVVLDKGTLDAIALSGIKFENNETVVDVYPKVIEKIL-GENSILLITS 183
D+ Q G+FDVV DKGT DA++L +N + +Y +EK+L +S+ +ITS
Sbjct: 116 DLTQPQNELGQFDVVHDKGTYDAVSLCP---DNAKEKRALYLDTVEKLLRTADSLFVITS 172
Query: 184 CNFXXXXXXXXXXXXXXXXWKSVNYPIFEFGGVKGTTICSVAF 226
CN+ + ++ P F+FGG G + S+ F
Sbjct: 173 CNWTEDELVDSFAEKFVKYY-TIPTPTFKFGGKVGNVVTSIVF 214
>CE27144 [R] KOG1271 Methyltransferases
Length = 236
Score = 107 bits (268), Expect = 1e-23
Identities = 74/246 (30%), Positives = 122/246 (49%), Gaps = 33/246 (13%)
Query: 2 EDTTKLNTSKLGTKEYWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLGEY 61
E + ++ +S+LGTK++WD Y LE +NF+++ D GE WF +E ++V++L+D+
Sbjct: 6 EQSVEIASSQLGTKDFWDQRYELELKNFKQH-GDEGEVWFG-TSSETRIVKYLIDS---- 59
Query: 62 NIKEDSSMIDLGTGNGHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEILKTKYSQND---- 117
+D+ ++DLG GNG +L L +GF+ + GVDY +K+V+ +A K + + +
Sbjct: 60 KTGKDAKILDLGCGNGSVLRKLRSKGFQ-SLKGVDYCQKAVDLSAAASKAEREEEEDEEL 118
Query: 118 --------NVTFATADIFNEQWAPGKFDVVLDKGTLDAIALSGIKFENNETVVDVYPKVI 169
++T AD F+ KFDV+LDKGT DA++LS + E + Y +
Sbjct: 119 VDIEFEQLDITTPPADFFS-----SKFDVILDKGTWDAMSLS----DEREARLKAYLGFL 169
Query: 170 EKILGENSILLITSCNFXXXXXXXXXXXXXXXXWKSVNYPI-----FEFGGVKGTTICSV 224
+ L +I SCNF + + F FGG +G T
Sbjct: 170 DNGLSAGGRFVIFSCNFTFDEMCSQFGGGGGSTSLEIVCEVPASHSFSFGGKQGVTSTGA 229
Query: 225 AFLKSS 230
F K +
Sbjct: 230 VFRKKT 235
>At1g66680 [R] KOG1271 Methyltransferases
Length = 358
Score = 88.6 bits (218), Expect = 7e-18
Identities = 71/199 (35%), Positives = 98/199 (48%), Gaps = 35/199 (17%)
Query: 10 SKLGTKEYWDDFYSLEKQNFEENPEDTGECWFADNDAE-------EKMVEFLLDNLG--- 59
S LG + YWD YS E NF E+ GE WF D+ E + VE N+
Sbjct: 95 SMLGLQSYWDAAYSDELTNFREHGH-AGEVWFGDDVMEIVTSWTKDLCVEISQRNMSVSE 153
Query: 60 -----EYNIKEDS-----SMIDLGTGNGHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEIL 109
E N + D +++DLGTGNG LL L +EGF ++ G DYS+ +VE A +
Sbjct: 154 NDVTTEVNDQADKYLSSWNVLDLGTGNGLLLHQLAKEGF-SDLTGTDYSDGAVELAQHL- 211
Query: 110 KTKYSQND---NVTFATADIFNEQWAPGKFDVVLDKGTLDAIALSGIKFENNETVVDVYP 166
SQ D N+ F DI + + +F +V+DKGTLDAI L + +Y
Sbjct: 212 ----SQRDGFPNIRFMVDDILDTK-LEQQFKLVMDKGTLDAIGL----HPDGPVKRVMYW 262
Query: 167 KVIEKILGENSILLITSCN 185
+ K++ IL+ITSCN
Sbjct: 263 DSVSKLVAPGGILVITSCN 281
>CE06739 [E] KOG2352 Predicted spermine/spermidine synthase
Length = 656
Score = 54.3 bits (129), Expect = 1e-07
Identities = 42/170 (24%), Positives = 74/170 (42%), Gaps = 29/170 (17%)
Query: 17 YWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLGEYNIKEDSSMIDLGTGN 76
YW +F++ K FE W+ D ++ L N+ + +K + + LG GN
Sbjct: 15 YWKNFFAKRKSPFE---------WYGDYNS--------LSNVIDKYLKPKDTFLQLGCGN 57
Query: 77 GHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEILKTKYSQNDN---VTFATADIFNEQWAP 133
L L + GF + S++ ++ T+ +N +TF T D N A
Sbjct: 58 SELATQLYDNGF--------HCIHSIDVEPSVIATQIRKNKERLGMTFETGDAANLSMAD 109
Query: 134 GKFDVVLDKGTLDAIALSGIKFENNETVVDVYPKVIEKILGENSILLITS 183
+V+DKGTLDA+ L E++E +V + + ++L +I +
Sbjct: 110 EAHTIVIDKGTLDAL-LPPSASESDEALVTKMFEEVHRVLASGGRYIIVT 158
>Hs14150112 [E] KOG2352 Predicted spermine/spermidine synthase
Length = 255
Score = 53.1 bits (126), Expect = 3e-07
Identities = 48/171 (28%), Positives = 80/171 (46%), Gaps = 23/171 (13%)
Query: 16 EYWDDFYSLEKQNFEENPEDTGECWFADNDAEEKMVEFLLDNLGEYNIKEDSSMIDLGTG 75
EYWD Q ++ + WF D + ++E ++ + ++ LG G
Sbjct: 24 EYWD-------QRYQGAADSAPYDWFGDFSSFRALLE--------PELRPEDRILVLGCG 68
Query: 76 NGHLLFTLLEEGFKGEMIGVDYSEKSVEFAAEILKTKYSQNDNVTFATADIFNEQWAPGK 135
N L + L GF + VDYS SV AA ++ +Y+ + + T D+ +
Sbjct: 69 NSALSYELFLGGFPN-VTSVDYS--SVVVAA--MQARYAHVPQLRWETMDVRKLDFPSAS 123
Query: 136 FDVVLDKGTLDAIALSGIKFENNETVVDVYPKVIEKILGENSILLITSCNF 186
FDVVL+KGTLDA+ L+G + + TV ++++L E S +L+ F
Sbjct: 124 FDVVLEKGTLDAL-LAGER--DPWTVSSEGVHTVDQVLSEVSRVLVPGGRF 171
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,368,702
Number of Sequences: 60738
Number of extensions: 560304
Number of successful extensions: 1193
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1175
Number of HSP's gapped (non-prelim): 8
length of query: 232
length of database: 30,389,216
effective HSP length: 103
effective length of query: 129
effective length of database: 24,133,202
effective search space: 3113183058
effective search space used: 3113183058
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)