ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII1971 good E KOG2436 Amino acid transport and metabolism Acetylglutamate kinase/acetylglutamate synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII1971 703591 701024 -856
(856 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER069w_1 [E] KOG2436 Acetylglutamate kinase/acetylglutamate syn... 749 0.0
YER069w_2 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase 515 e-145
SPAC4G9.09c_1 [E] KOG2436 Acetylglutamate kinase/acetylglutamate... 453 e-127
SPAC4G9.09c_2 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate redu... 417 e-116
At2g19940 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase 110 1e-23
Hs20559071 [E] KOG2436 Acetylglutamate kinase/acetylglutamate sy... 105 2e-22
At3g57560 [E] KOG2436 Acetylglutamate kinase/acetylglutamate syn... 94 1e-18
At4g37670 [E] KOG2436 Acetylglutamate kinase/acetylglutamate syn... 53 2e-06
>YER069w_1 [E] KOG2436 Acetylglutamate kinase/acetylglutamate synthase
Length = 541
Score = 749 bits (1934), Expect = 0.0
Identities = 389/546 (71%), Positives = 432/546 (78%), Gaps = 21/546 (3%)
Query: 3 VPSARLLMSTKGLNPSRLSKV------------ASILARCSPSIP---KRVGYSKRFVSS 47
+PSA LL+STK LN S+ K AS+ R PS+ K+VGYSKR+VSS
Sbjct: 1 MPSASLLVSTKRLNASKFQKFVSSLNKSTIAGFASVPLRAPPSVAFTRKKVGYSKRYVSS 60
Query: 48 ANGVGSTGTRSTVIQLLNNIGSKREVEQYLKYFTSVSQQQFAVIKVGGAIISDNLQELAS 107
NG +T RSTVIQLLNNI +KREVEQYLKYFTSVSQQQFAVIKVGGAIISDNL ELAS
Sbjct: 61 TNGFSAT--RSTVIQLLNNISTKREVEQYLKYFTSVSQQQFAVIKVGGAIISDNLHELAS 118
Query: 108 CLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRITDDHTMAVVRQCFLEQNLK 167
CLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRITD+HTMAVVR+CFLEQNLK
Sbjct: 119 CLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRITDEHTMAVVRKCFLEQNLK 178
Query: 168 LVTALEQLGVRARPITSGVFTAEYLDKDKYKLVGDITSVARDPIEASIKAGVLPILTSLA 227
LVTALEQLGVRARPITSGVFTA+YLDKDKYKLVG+I SV ++PIEASIKAG LPILTSLA
Sbjct: 179 LVTALEQLGVRARPITSGVFTADYLDKDKYKLVGNIKSVTKEPIEASIKAGALPILTSLA 238
Query: 228 ETPSGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEKVSVINLDEEYDELMK 287
ET SGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEK+S+INLDEEYD+LMK
Sbjct: 239 ETASGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEKISMINLDEEYDDLMK 298
Query: 288 QSWVKFGTXXXXXXXXXXXXXXPRSSSVAIINVQDLQKELFTDSGAGTLIRRGYKLIRRS 347
QSWVK+GT PRSSSVAIINVQDLQKELFTDSGAGT+IRRGYKL++RS
Sbjct: 299 QSWVKYGTKLKIREIKELLDYLPRSSSVAIINVQDLQKELFTDSGAGTMIRRGYKLVKRS 358
Query: 348 TLQEFPSSDALRDILQSDPDIQSGAQSVASYLRDLNESDFTSYADEPLDVLAIVKKDSVV 407
++ EFPS+DALR LQ D I SG +SVASYLR L SDF SYADEPL+ +AIVKKD+ V
Sbjct: 359 SIGEFPSADALRKALQRDAGISSGKESVASYLRYLENSDFVSYADEPLEAVAIVKKDTNV 418
Query: 408 AKLDKFLCSQTGWLNNVTDNVFKALKRDFPALQWVINEDDANIAWHFNKSDGSYLKNGKV 467
LDKF+CS WLNNVTDNVF L+RDFPALQWV++E+DANIAWHF+KS GSYLK GKV
Sbjct: 419 PTLDKFVCSDAAWLNNVTDNVFNVLRRDFPALQWVVSENDANIAWHFDKSQGSYLKGGKV 478
Query: 468 LFWYGVKDLNTVSELISEFIKTSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPKVE 527
LFWYG+ D+NT+SEL+ F+K+ P+ E
Sbjct: 479 LFWYGIDDINTISELVENFVKSC----DTASTLNSSASSGVFANKKSARSYSTRSTPRPE 534
Query: 528 GTNKSP 533
G N +P
Sbjct: 535 GVNTNP 540
>YER069w_2 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase
Length = 322
Score = 515 bits (1326), Expect = e-145
Identities = 246/321 (76%), Positives = 287/321 (88%), Gaps = 1/321 (0%)
Query: 535 KVALIGARGYTGQNLISLIDSHPFLELSHVSSRQNAGKKLEGYNKSEIIYSNLQIDDIKK 594
+VALIGARGYTG+NL+SLI+ HP+LE++HVSSR+ G+KL+ Y KSEIIY +LQI DI+K
Sbjct: 1 RVALIGARGYTGKNLVSLINGHPYLEVAHVSSRELKGQKLQDYTKSEIIYESLQIQDIRK 60
Query: 595 LESEGAVDVWVMALPNKVCEPFVEAIESVANGKSKIVDLSADHRFVPETEWSYGLPELNN 654
LE + AVD WVMALPNKVCEPFVE I+SV +GKSKI+DLSADHRFV E++W+YGLPELN+
Sbjct: 61 LEEQNAVDFWVMALPNKVCEPFVETIQSV-HGKSKIIDLSADHRFVSESDWAYGLPELND 119
Query: 655 REAIANAKKIANPGCYATGAQVAIAPLSEYITGLPTVFGVSGYSGAGTTPSPKNDPKFLN 714
R IANA KIANPGCYATG+Q+ I+PL++YI GLPTVFGVSGYSGAGT PSPKNDPKFLN
Sbjct: 120 RAKIANAAKIANPGCYATGSQLTISPLTKYINGLPTVFGVSGYSGAGTKPSPKNDPKFLN 179
Query: 715 NNLIPYSLTNHIHEREISTRLGLEVAFVPHVAQWFQGISHTISIPIKKNSLSSEQIKALY 774
NNLIPY+L++HIHEREIS R+G VAF+PHV QWFQGIS T+SIPIKK SLS ++I+ LY
Sbjct: 180 NNLIPYALSDHIHEREISARIGHNVAFMPHVGQWFQGISLTVSIPIKKGSLSIDEIRKLY 239
Query: 775 TEFYANEKLVHVADDIPLVKDVSGTHGVVVGGFKVNDAKDRVVVCATIDNLLKGAATQCL 834
FY +EKLVHV DDIPLVKD+ GTHGVV+GGFK+NDA+DRVVVCATIDNLLKGAATQCL
Sbjct: 240 RNFYEDEKLVHVIDDIPLVKDIEGTHGVVIGGFKLNDAEDRVVVCATIDNLLKGAATQCL 299
Query: 835 QNINLALSYGEYDGIPDNKII 855
QNINLA+ YGEY GIP+NKII
Sbjct: 300 QNINLAMGYGEYAGIPENKII 320
>SPAC4G9.09c_1 [E] KOG2436 Acetylglutamate kinase/acetylglutamate synthase
Length = 551
Score = 453 bits (1165), Expect = e-127
Identities = 233/428 (54%), Positives = 300/428 (69%), Gaps = 2/428 (0%)
Query: 57 RSTVIQLLNNIGSKREVEQYLKYFTSVSQQQFAVIKVGGAIISDNLQELASCLAFLYHVG 116
R +I++L++IGS+REVEQYL+YFTS Q+FA+IKVGGAII+D L LA LAFL HVG
Sbjct: 62 RDAIIRILSSIGSRREVEQYLRYFTSFEAQRFAIIKVGGAIITDELDTLAQSLAFLNHVG 121
Query: 117 LYPIVLHGTGPQVNGRLEAQGIEPDYIDGIRITDDHTMAVVRQCFLEQNLKLVTALEQLG 176
LYPIV+HG GPQ+N L ++ +EP+Y DGIRITD T++V R+ FLE+N KLV ALE+LG
Sbjct: 122 LYPIVVHGAGPQLNKILASRNVEPEYSDGIRITDAETLSVARKVFLEENAKLVDALEKLG 181
Query: 177 VRARPITSGVFTAEYLDKDKYKLVGDITSVARDPIEASIKAGVLPILTSLAETPSGQMLN 236
RARPIT GVF AEYLDK+KYK VG I V + PIE SI+AG LPILTS+AET SGQ+LN
Sbjct: 182 TRARPITGGVFQAEYLDKEKYKYVGKIVKVNKAPIEHSIRAGTLPILTSMAETASGQLLN 241
Query: 237 VNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEKVSVINLDEEYDELMKQSWVKFGTX 296
VNAD+ AGELARV +PLK+VYLNEKGG+ING T +K+S I LD EYD LMKQ WVK+GT
Sbjct: 242 VNADITAGELARVLKPLKVVYLNEKGGLINGETKKKISSIYLDREYDGLMKQPWVKYGTK 301
Query: 297 XXXXXXXXXXXXXPRSSSVAIINVQDLQKELFTDSGAGTLIRRGYKLIRRSTLQEFPSSD 356
PR+SSVAII+ +DLQKELFT+SGAGTLI RG+ + + +L P +
Sbjct: 302 LKIKEIKELLDTLPRTSSVAIISTKDLQKELFTESGAGTLISRGFVINKHDSLDSIPDA- 360
Query: 357 ALRDILQSDPDIQSGAQSVASYLRDLNESDFTSYADEPLDVLAIV-KKDSVVAKLDKFLC 415
AL +++ + + ++S+ + L + Y+D + +AIV DS + L F
Sbjct: 361 ALENLIIQKNSLAAPSESLKQFKDTLKDRKLRIYSDSFNESVAIVDTTDSSLPVLLAFGA 420
Query: 416 SQTGWLNNVTDNVFKALKRDFPALQWVINEDDANIAWHFNKSDGSYLKNGKVLFWYGVKD 475
+ WLNNV D++ LK DFP+L W + N+ W F+KS+G+ N FWYGVKD
Sbjct: 421 ADNNWLNNVVDSILTTLKADFPSLLWRLQPSAKNLEWFFSKSEGTLFANNFYYFWYGVKD 480
Query: 476 LNTVSELI 483
LN +S+ I
Sbjct: 481 LNKISKFI 488
>SPAC4G9.09c_2 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase
Length = 334
Score = 417 bits (1071), Expect = e-116
Identities = 207/324 (63%), Positives = 252/324 (76%), Gaps = 4/324 (1%)
Query: 526 VEGTNKSPAKVALIGARGYTGQNLISLIDSHPFLELSHVSSRQNAGKKLEGYNKSEIIYS 585
++ + PAKV L+GARGYTG+NLI LI++HP+LELSHVSSR+ G KL GY K EI Y
Sbjct: 12 LKAVSSKPAKVVLLGARGYTGKNLIGLINTHPYLELSHVSSRELEGTKLPGYTKKEIQYV 71
Query: 586 NLQIDDIKKLESEGAVDVWVMALPNKVCEPFVEAIESVANGKSKIVDLSADHRFVPETEW 645
NL DD+KKLE EGAVD WVMALPN VC+P+V+A+ S ANGKS IVDLSAD+RF P W
Sbjct: 72 NLSTDDVKKLEEEGAVDAWVMALPNGVCKPYVDALTS-ANGKSVIVDLSADYRFEPS--W 128
Query: 646 SYGLPELNNREAIANAKKIANPGCYATGAQVAIAPLSEYITGLPTVFGVSGYSGAGTTPS 705
YGLPELN+REA+ N+K+I+NPGCYATG+QV + P+ I G P++FGVSGYSGAGT PS
Sbjct: 129 QYGLPELNDREALRNSKRISNPGCYATGSQVGLTPILSLIDGQPSIFGVSGYSGAGTKPS 188
Query: 706 PKNDPKFLNNNLIPYSLTNHIHEREISTRLGLEVAFVPHVAQWFQGISHTISIPIKKNSL 765
PKND L NNLIPYSLT+HIHEREIS RL VAF+PHVAQWFQGI+ TI++P+KK S+
Sbjct: 189 PKNDLNVLTNNLIPYSLTDHIHEREISYRLKQPVAFIPHVAQWFQGITLTINVPLKK-SI 247
Query: 766 SSEQIKALYTEFYANEKLVHVADDIPLVKDVSGTHGVVVGGFKVNDAKDRVVVCATIDNL 825
+S +++ LY + Y E L+HV D+PLVKD + H V VGGF V+ + R V+ TIDNL
Sbjct: 248 TSRELRNLYQDRYNGEPLIHVQGDVPLVKDNAHKHHVSVGGFGVHSSGKRAVIVVTIDNL 307
Query: 826 LKGAATQCLQNINLALSYGEYDGI 849
LKGAATQ LQN+NL+ Y EY GI
Sbjct: 308 LKGAATQALQNLNLSCGYDEYAGI 331
>At2g19940 [E] KOG4354 N-acetyl-gamma-glutamyl-phosphate reductase
Length = 389
Score = 110 bits (274), Expect = 1e-23
Identities = 94/343 (27%), Positives = 156/343 (45%), Gaps = 40/343 (11%)
Query: 535 KVALIGARGYTGQNLISLIDSHPFLELSHVSSRQNAGKKLEGYNKSEIIYSNLQIDDIKK 594
++ L+GA GYTG ++ L+ +HP +++ +++ + AG+ +E ++ +L+ D
Sbjct: 52 RIGLLGASGYTGAEIVRLLANHPHFQVTLMTADRKAGQSMES------VFPHLRAQDA-- 103
Query: 595 LESEGAVDVWVMALPNKVCE---PFVEAIESVANGKSKIVDLSADHRFVPETEWSYGLP- 650
VD LP+ + PF+ + + S R + E E YG P
Sbjct: 104 --DFSTVDAVFCCLPHGTTQVHFPFILPFQFLKAFLSLTEMSDFRLRNIAEYEEWYGQPH 161
Query: 651 ---ELNN----------REAIANAKKIANPGCYATGAQVAIAPL--SEYITGLPTVFGV- 694
EL RE I A+ +ANPGCY T Q+ + PL + I +
Sbjct: 162 KAVELQKEVVYGLTEILREDIKKARLVANPGCYPTTIQLPLVPLLKANLIKHENIIIDAK 221
Query: 695 SGYSGAGTTPSPKNDPKFLNNNLIPYSLTNHIHEREISTRLG------LEVAFVPHVAQW 748
SG SGAG N + + Y +T H H EI L + V+F PH+
Sbjct: 222 SGVSGAGRGAKEANLYSEIAEGISSYGVTRHRHVPEIEQGLSDVAQSKVTVSFTPHLMPM 281
Query: 749 FQGISHTISIPIKKNSLSSEQIKALYTEFYANEKLVHVADD--IPLVKDVSGTHGVVVGG 806
+G+ TI + + + + + L T Y +E+ V V D+ +P +V G++ +
Sbjct: 282 IRGMQSTIYVEMAPGVRTEDLHQQLKTS-YEDEEFVKVLDEGVVPRTHNVRGSNYCHMSV 340
Query: 807 FKVNDAKDRVVVCATIDNLLKGAATQCLQNINLALSYGEYDGI 849
F + R ++ + IDNL+KGA+ Q LQN+N+ L Y E G+
Sbjct: 341 FP-DRIPGRAIIISVIDNLVKGASGQALQNLNIMLGYPETTGL 382
>Hs20559071 [E] KOG2436 Acetylglutamate kinase/acetylglutamate synthase
Length = 376
Score = 105 bits (263), Expect = 2e-22
Identities = 82/328 (25%), Positives = 140/328 (42%), Gaps = 17/328 (5%)
Query: 168 LVTALEQLGVRARPITSGVFTAEYLDKDKYKLVGDITSVARDPIEASIKAGVLPILTSLA 227
LV AL A P G + + G I SV D ++ +++G +PIL +
Sbjct: 37 LVDALRHNAAAAVPFFGGGSVLRAAEPAPHASYGGIVSVETDLLQWCLESGSIPILCPIG 96
Query: 228 ETPSGQMLNVNADVAAGELARVFEPLKIVYLNEKGGIINGSTGEKVSVINLDEEYDELMK 287
ET + + + +++ LA+ P KI++LN GG + S+ + +S +NL + D +
Sbjct: 97 ETAARRSVLLDSLEVTASLAKALRPTKIIFLNNTGG-LRDSSHKVLSNVNLPADLDLVCN 155
Query: 288 QSWVKFGTXXXXXXXXXXXXXXPRSSSVAIINVQDLQKELFTDSGAGTLIRRGYKLIRRS 347
WV P SS I L ELF++ G+GTL + +++R
Sbjct: 156 AEWVSTKERQQMRLIVDVLSRLPHHSSAVITAASTLLTELFSNKGSGTLFKNAERMLRVR 215
Query: 348 TLQEFPSSDALRDILQSDPDIQSGAQSVASYLRDLNESDFTSYADEPL--------DVLA 399
+L + L D++ + G + YL L + Y E L + A
Sbjct: 216 SLDKL-DQGRLVDLVNA----SFGKKLRDDYLASLRPRLHSIYVSEGLPLSRCARYNAAA 270
Query: 400 IVKKDSVVA---KLDKFLCSQTGWLNNVTDNVFKALKRDFPALQWVINEDDANIAWHFNK 456
I+ + V+ LDKF+ S + +++ L+RD L W + W+F
Sbjct: 271 ILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKH 330
Query: 457 SDGSYLKNGKVLFWYGVKDLNTVSELIS 484
SDGS+ + FW+G+ D+ EL++
Sbjct: 331 SDGSFSNKQWIFFWFGLADIRDSYELVN 358
>At3g57560 [E] KOG2436 Acetylglutamate kinase/acetylglutamate synthase
Length = 347
Score = 93.6 bits (231), Expect = 1e-18
Identities = 71/257 (27%), Positives = 121/257 (46%), Gaps = 9/257 (3%)
Query: 90 VIKVGGAIIS--DNLQELASCLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIR 147
V+K GGA ++ + + S L L VGL PI++HG GP +N L+ I ++ DG+R
Sbjct: 89 VVKYGGAAMTSPELKSSVVSDLVLLACVGLRPILVHGGGPDINRYLKQLNIPAEFRDGLR 148
Query: 148 ITDDHTMAVVRQCFLEQ-NLKLVTALEQLGVRARPITSG---VFTAEYLDKD-KYKLVGD 202
+TD TM +V + + N LV+ + G A ++ + TA + + VG+
Sbjct: 149 VTDATTMEIVSMVLVGKVNKNLVSLINAAGATAVGLSGHDGRLLTARPVPNSAQLGFVGE 208
Query: 203 ITSVARDPIEASIKAGVLPILTSLAETPSGQMLNVNADVAAGELARVFEPLKIVYLNEKG 262
+ V + + G +P++ S+A SGQ N+NAD AGELA K++ L +
Sbjct: 209 VARVDPSVLRPLVDYGYIPVIASVAADDSGQAYNINADTVAGELAAALGAEKLILLTDVA 268
Query: 263 GIINGSTGEKVSVINLD-EEYDELMKQSWVKFGTXXXXXXXXXXXXXXPRSSSVAIINVQ 321
GI+ + +D + ++++ V G +++S+ Q
Sbjct: 269 GILENKEDPSSLIKEIDIKGVKKMIEDGKVAGGMIPKVKCCIRSLAQGVKTASIIDGRRQ 328
Query: 322 -DLQKELFTDSGAGTLI 337
L E+ +D GAGT+I
Sbjct: 329 HSLLHEIMSDEGAGTMI 345
>At4g37670 [E] KOG2436 Acetylglutamate kinase/acetylglutamate synthase
Length = 571
Score = 52.8 bits (125), Expect = 2e-06
Identities = 50/214 (23%), Positives = 90/214 (41%), Gaps = 25/214 (11%)
Query: 88 FAVIKVGGAIISDNLQELASCLAFLYHVGLYPIVLHGTGPQVNGRLEAQGIEPDYIDGIR 147
F VI G I + + +AFL+H+G+ +++ GT Q++ L +G E Y+ R
Sbjct: 103 FVVIISGEIIAGSSCDAILKDIAFLHHLGIRFVLVPGTQEQIDQLLAERGREATYVGRYR 162
Query: 148 ITDDHTMAVVRQC------FLEQNLKL------------VTALEQLGVRARPITSGVFTA 189
+TD ++ ++ LE L + L +GVR T F A
Sbjct: 163 VTDAASLQAAKEAAGAISVMLEAKLSPGPSICNIRRHGDRSRLHDIGVRVD--TGNFFAA 220
Query: 190 E---YLDKDKYKLVGDITSVARDPIEASIKAGVLPILTSLAETPSGQMLNVNADVAAGEL 246
+ +D + G++ + D I + G + +L +L + SG++LN N A
Sbjct: 221 KRRGVVDGVDFGATGEVKKIDVDRICERLDGGSVVLLRNLGHSSSGEVLNCNTYEVATAC 280
Query: 247 ARVFEPLKIVYLNEKGGIINGSTGEKVSVINLDE 280
A K++ + + G I +G + + L E
Sbjct: 281 ALAIGADKLICIMD--GPILDESGHLIHFLTLQE 312
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.134 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 47,786,452
Number of Sequences: 60738
Number of extensions: 1997992
Number of successful extensions: 5198
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 5177
Number of HSP's gapped (non-prelim): 8
length of query: 856
length of database: 30,389,216
effective HSP length: 115
effective length of query: 741
effective length of database: 23,404,346
effective search space: 17342620386
effective search space used: 17342620386
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)