ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2046 good I KOG1640 Lipid transport and metabolism Predicted steroid reductase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2046 725100 724402 -233
(233 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YIL049w [I] KOG1640 Predicted steroid reductase 147 1e-35
SPAC7D4.09c [I] KOG1640 Predicted steroid reductase 74 1e-13
Hs13375785 [I] KOG1640 Predicted steroid reductase 63 3e-10
At5g16010 [I] KOG1638 Steroid reductase 53 3e-07
CE05157 [I] KOG1640 Predicted steroid reductase 53 4e-07
>YIL049w [I] KOG1640 Predicted steroid reductase
Length = 253
Score = 147 bits (371), Expect = 1e-35
Identities = 86/238 (36%), Positives = 138/238 (57%), Gaps = 20/238 (8%)
Query: 11 TFYVGIISVFVAEFYIPQLLKYGKTWEASNNFWYS--------LTCPKSNFGHFYLLSSI 62
+F+VGI S+F+A+ +P+ L+YGKT+ N YS T PK+ F HFY L++
Sbjct: 19 SFFVGICSLFIAKSCLPEFLQYGKTYRPKENSKYSSILERIKKFTVPKAYFSHFYYLATF 78
Query: 63 FSIYNLLWIRSL-LTLSVFIHSVRRLYECYCVTKW-GQSKIHLSHYLVGIWFYTTLNL-- 118
S+ L + + +F HS+RRLYE V + S+++ SHYLVGIWFY+ L L
Sbjct: 79 LSLVTLYFYPKFPIVWIIFGHSLRRLYETLYVLHYTSNSRMNWSHYLVGIWFYSVLLLIL 138
Query: 119 GIMLYHGETQYSLI--SVILFIMSSLDQATNHDYLSKLKKYSQPSYGLFKYICSAHYFDE 176
I LY +L + I+F ++S DQ NH L+ L KYS P+ LF+ +C HY DE
Sbjct: 139 NISLYKNSIPNTLNMNAFIIFCIASWDQYKNHVILANLVKYSLPTGRLFRLVCCPHYLDE 198
Query: 177 LLIYTSFMLMDKGSRMLLIHCILWILVNLGTSSFETGNWYQEKF--GYRSRWYLIPYV 232
++IY++ + ++ + L+ W++ +L S+ ET N+Y+ KF + + + +IP++
Sbjct: 199 IIIYSTLLPYEQEFYLTLV----WVITSLTISALETKNYYRHKFKDNHVAPYAIIPFI 252
>SPAC7D4.09c [I] KOG1640 Predicted steroid reductase
Length = 274
Score = 74.3 bits (181), Expect = 1e-13
Identities = 74/269 (27%), Positives = 122/269 (44%), Gaps = 48/269 (17%)
Query: 5 QILLYSTFYVGIISVFVAEFYIPQLLKYGKTWEASNNFWYS-------LTCPKSNFGHFY 57
++ Y T +S+ + L+KYG +NF L PK F HFY
Sbjct: 14 EVAAYFTSTTLFVSILKNAPSLSWLMKYG----GHDNFGLKPPLIATKLEVPKRWFWHFY 69
Query: 58 ----LLSSIFSIY----------------NLLWIRSLLTLSVF--IHSVRRLYECYCVTK 95
LL+ +F+ + +L++ + L L + IH++RRLYE K
Sbjct: 70 AFISLLNPLFTFFILNTNFPIPIFKNIKEDLMYSKKLQVLLLIYEIHTLRRLYENLRFRK 129
Query: 96 WGQSKIHLSHYLVGIWFYTTLNLGIMLYHGETQYSLISVILFI------MSSLDQATNHD 149
G SK+ HYL+G FYT L ++L + + +S + F+ + S+ Q +H+
Sbjct: 130 SG-SKMLAGHYLLGYLFYTHTFLALLLCGRRSYENTMSSMQFVGLGIYAIGSIWQNASHE 188
Query: 150 YLSKLKKYSQP---SYGLFKYICSAHYFDELLIYTSFMLMDKGSRMLLIHCIL-WILVNL 205
+L K +SQ G FK+I HY E+++YT L+ ++ LI +L W+L N+
Sbjct: 189 HLIAQKNHSQYLVLKKGCFKWITGPHYLGEIIVYTGIALI---AQHWLIWLVLGWVLCNM 245
Query: 206 -GTSSFETGNWYQEKFGYRSRWYLIPYVF 233
SS ++ RW LIP+++
Sbjct: 246 VAISSSYACTVKNKEQSLDFRWTLIPFLY 274
>Hs13375785 [I] KOG1640 Predicted steroid reductase
Length = 318
Score = 63.2 bits (152), Expect = 3e-10
Identities = 71/276 (25%), Positives = 114/276 (40%), Gaps = 77/276 (27%)
Query: 29 LLKYGKT--WEASNNF-WYSLTCPKSNFGHFYLLSSIFSIYNLL--------------WI 71
L++YGKT E S + PK F HFY++S +++ + L W+
Sbjct: 49 LIRYGKTKCGEPSRPAACRAFDVPKRYFSHFYIISVLWNGFLLWCLTQSLFLGAPFPSWL 108
Query: 72 RSLL-------------TLSVFI-------HSVRRLYECYCVTKWGQSKIHLSHYLVGIW 111
LL LS F+ HS+RRL+EC V+ + IH+ Y G+
Sbjct: 109 HGLLRILGAAQFQGGELALSAFLVLVFLWLHSLRRLFECLYVSVFSNVMIHVVQYCFGLV 168
Query: 112 FYTTLNLGIM----------------LYHGETQYSLISVILFIMSSLDQATNHDYLSKLK 155
+Y + L ++ L + ++ +++FI SS Q H L L+
Sbjct: 169 YYVLVGLTVLSQVPMDGRNAYITGKNLLMQARWFHILGMMMFIWSSAHQYKCHVILGNLR 228
Query: 156 K----------YSQPSYGLFKYICSAHYFDELLIYTSFMLMDKGSRMLLIHCILWILV-- 203
K + P F+Y+ S +Y EL+IY S M + G H + W LV
Sbjct: 229 KNKAGVVIHCNHRIPFGDWFEYVSSPNYLAELMIYVS-MAVTFG-----FHNLTWWLVVT 282
Query: 204 ----NLGTSSFETGNWYQEKF-GY-RSRWYLIPYVF 233
N S+F + +Y+ KF Y + R +P++F
Sbjct: 283 NVFFNQALSAFLSHQFYKSKFVSYPKHRKAFLPFLF 318
>At5g16010 [I] KOG1638 Steroid reductase
Length = 268
Score = 53.1 bits (126), Expect = 3e-07
Identities = 46/175 (26%), Positives = 79/175 (44%), Gaps = 17/175 (9%)
Query: 74 LLTLSVFIHSVRRLYECYCVTKW-GQSKIHLSHYLVGIWFYTTL------NLGIMLYHGE 126
LL ++ +H +R++E + K+ G I + + +F +T NL + L
Sbjct: 96 LLKSALALHFFKRVFEVLFIHKYSGGMAIDSALTISSSYFSSTALMLYSQNLTLGLTEPS 155
Query: 127 TQYSLISVILFIMSSLDQATNHDYLSKLKK------YSQPSYGLFKYICSAHYFDELLIY 180
L V++F++ + +H L+KL+K Y P GLF I HY E+L++
Sbjct: 156 FDMKLAGVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVF 215
Query: 181 TSFMLMDKGSRMLLIHCILWILVNLGTSSFETGNWYQEKFGYRSRWY--LIPYVF 233
SF L+ + + + ++ L S+ T WY KF + LIP+VF
Sbjct: 216 WSFFLISQ--TIYSFSFAMGTMLYLIGRSYATRTWYLSKFDDFPKHIKALIPFVF 268
>CE05157 [I] KOG1640 Predicted steroid reductase
Length = 342
Score = 52.8 bits (125), Expect = 4e-07
Identities = 49/186 (26%), Positives = 80/186 (42%), Gaps = 28/186 (15%)
Query: 74 LLTLSVFIHSVRRLYECYCVTKWGQSKIHLSHYLVGIWFYTTLNLGIM------------ 121
L L + +H RRLYE V+ + S+++L HY VGI Y L + IM
Sbjct: 157 LALLLISLHVARRLYETIFVSVYSDSRMNLFHYAVGIVHYIILPISIMCETQGVASKLPQ 216
Query: 122 LYHGETQYSL---ISVILFIMSSLDQATNHDYLSKLKK----------YSQPSYGLFKYI 168
L+ SL +LF + + Q + ++ +K Y G F +
Sbjct: 217 LHVSIDDISLTQWAGAVLFWICNWKQHQLAEQIANTRKGPRGLIRNYAYGICFGGWFNLV 276
Query: 169 CSAHYFDELLIYTSFMLMDKGSRMLLIHCILWILVNLGTSSFETGNWYQEKFGY--RSRW 226
H+ E+ IY S L+ + + I+++ +N ++ T +WY + F +SR
Sbjct: 277 SCPHFLFEICIYLSLFLVIPDAYVYRF-IIMFVCINQTFAALITHSWYHKTFPKYPKSRK 335
Query: 227 YLIPYV 232
LIPYV
Sbjct: 336 ALIPYV 341
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.330 0.143 0.469
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,396,252
Number of Sequences: 60738
Number of extensions: 597738
Number of successful extensions: 1788
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1775
Number of HSP's gapped (non-prelim): 7
length of query: 233
length of database: 30,389,216
effective HSP length: 103
effective length of query: 130
effective length of database: 24,133,202
effective search space: 3137316260
effective search space used: 3137316260
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)