ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2120 good MU KOG2516 Cell wall/membrane/envelope biogenesis Protein involved in dolichol pathway for N-glycosylation (mannosyltransferase family)
r_klactIII2120 good MU KOG2516 Intracellular trafficking, secretion, and vesicular transport Protein involved in dolichol pathway for N-glycosylation (mannosyltransferase family)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2120 753881 752259 -541
(541 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNR030w [MU] KOG2516 Protein involved in dolichol pathway for N-... 428 e-119
SPBC1734.12c [MU] KOG2516 Protein involved in dolichol pathway f... 209 1e-53
7299245 [MU] KOG2516 Protein involved in dolichol pathway for N-... 199 9e-51
Hs13129114 [MU] KOG2516 Protein involved in dolichol pathway for... 197 3e-50
CE29152 [MU] KOG2516 Protein involved in dolichol pathway for N-... 165 1e-40
>YNR030w [MU] KOG2516 Protein involved in dolichol pathway for
N-glycosylation (mannosyltransferase family)
Length = 551
Score = 428 bits (1100), Expect = e-119
Identities = 230/544 (42%), Positives = 323/544 (59%), Gaps = 17/544 (3%)
Query: 7 LTTVLTCAVSLHLWYSPYTKVEESFTLQAVHDILTYGVFDVSNYDHVTFAGAVPRSFVGP 66
L TVL +S HL +P+TKVEESF +QA+HDILTY VFD+S YDH+ F G VPR+FVG
Sbjct: 6 LDTVLLTVISFHLIQAPFTKVEESFNIQAIHDILTYSVFDISQYDHLKFPGVVPRTFVGA 65
Query: 67 LILSALTYPF----NLTISKDP--FRTQMLVRFLIGLTNTVALNRFIVAAKKALTKDQXX 120
+I++ L+ P+ +L + P Q++VR ++GLTN ++ + K L
Sbjct: 66 VIIAMLSRPYLYLSSLIQTSRPTSIDVQLVVRGIVGLTNGLSF----IYLKNCLQDMFDE 121
Query: 121 XXXXXXXXXXXXXXXXASDLTNWFGLFTATQFHLMFYSSRPLPNFVMXXXXXXXXXXXXX 180
WF LF FHLMFYS+R LPNFVM
Sbjct: 122 ITEKKKEENEDKDIYIYDSAGTWFLLFLIGSFHLMFYSTRTLPNFVMTLPLTNVALGWVL 181
Query: 181 EENYRWSIILLSFTAIVFRLEIXXXXXXXXXXXFFYEKISLTSIVRTGFIGAGAGIFLSI 240
Y +I L + AIVFRLE+ ++KISL ++ G G G G +SI
Sbjct: 182 LGRYNAAIFLSALVAIVFRLEVSALSAGIALFSVIFKKISLFDAIKFGIFGLGLGSAISI 241
Query: 241 QVDSYFWNRLTVPEIEAFIFNVVHGESSKWGTQPFFAYLTGYLPMMFIPPTALLLNHLGF 300
VDSYFW +PE++ F+FNVV G +SKWG +P AY T YL MMF+PPT LLLN+ G+
Sbjct: 242 TVDSYFWQEWCLPEVDGFLFNVVAGYASKWGVEPVTAYFTHYLRMMFMPPTVLLLNYFGY 301
Query: 301 KIGPNSFRVVGIAAYFHVLVMSLQPHKEWRFIIYSNPPITLLGATAAAYLFKNIDNKSVT 360
K+ P ++V +A+ FH++V+S QPHKEWRFIIY+ P I LLGAT AA+L++N+ K +T
Sbjct: 302 KLAPAKLKIVSLASLFHIIVLSFQPHKEWRFIIYAVPSIMLLGATGAAHLWENMKVKKIT 361
Query: 361 GVAVRFVXXXXXXXXXFISIAFSYISTMNYPGGVALENFNEIIKSQNITDAVVHLDVPVC 420
V + FIS+AF YIS MNYPGG AL +FN++I +NIT+A VH+ +P C
Sbjct: 362 NVLCLAILPLSIMTSFFISMAFLYISRMNYPGGEALTSFNDMIVEKNITNATVHISIPPC 421
Query: 421 MTGATLFGQLD-DSWNITYDKTEDGT-LSSKWPSFNYLITPIDNSSLIPTED--GNRWEL 476
MTG TLFG+L+ + I YDKTE+ T L WPSF++LIT +S +P E+ N WEL
Sbjct: 422 MTGVTLFGELNYGVYGINYDKTENTTLLQEMWPSFDFLITHEPTASQLPFENKTTNHWEL 481
Query: 477 ISKSQGFGGINTEYLVKTFTEQYANGLDL--YVTWERSRDFTFIKEIFDNALAMKDVFYT 534
++ ++ F G + Y+ ++ N L L + ++++ F+KE+ N++ DVF+T
Sbjct: 482 VNTTKMFTGFDPTYIKNFVFQERVNVLSLLKQIIFDKT-PTVFLKELTANSIVKSDVFFT 540
Query: 535 YQKV 538
Y+++
Sbjct: 541 YKRI 544
>SPBC1734.12c [MU] KOG2516 Protein involved in dolichol pathway for
N-glycosylation (mannosyltransferase family)
Length = 547
Score = 209 bits (531), Expect = 1e-53
Identities = 152/453 (33%), Positives = 212/453 (46%), Gaps = 42/453 (9%)
Query: 10 VLTCAVSLHLWYSPYTKVEESFTLQAVHDILTYGVFDVSN-YDHVTFAGAVPRSFVGPLI 68
++TC + + + +P+TKVEESF +QA+HDI TY +D+S YDH+ F GAV RSF+ L
Sbjct: 16 LVTC-IGYYSYKTPFTKVEESFAMQAIHDIQTYR-WDLSKVYDHLEFPGAVKRSFIPSLF 73
Query: 69 LSALTYPFNLTISKDPFRTQMLVRFLIGLTNTVALNRFIVAAKKALTKDQXXXXXXXXXX 128
++ L+Y + ++ + R+ IG + ++N + K
Sbjct: 74 IAVLSYIPSWFVNP-----LLAARWTIGYLSWESMNSVSCSISKRFGTLSGAL------- 121
Query: 129 XXXXXXXXASDLTNWFGLFTATQFHLMFYSSRPLPNFVMXXXXXXXXXXXXXEENYRWSI 188
F LF+ QFHL++Y SRPL N + + NY SI
Sbjct: 122 ---------------FILFSCAQFHLVYYMSRPLSN-IFGLIATNHSLSLLLKNNYYGSI 165
Query: 189 ILLSFTAIVFRLEIXXXXXXXXXXXFFYEKISLTSIVRTGFIGAGAGIFLSIQVDSYFWN 248
+L F A + R EI +I+L+ ++ G + A + S +DSYFW
Sbjct: 166 SILVFAAAIVRSEIALLLMCLILPLLLQRRITLSKLLLVGISSSLAAVGASFLIDSYFWG 225
Query: 249 RLTVPEIEAFIFNVVHGESSKWGTQPFFAYLTGYLPMMFIPPTALLLNHLGFKIGPNSFR 308
PE+EAF+FNVV G+SS WGT PF+ Y LP +F+ PT LL + F + R
Sbjct: 226 AWCWPELEAFLFNVVEGKSSDWGTSPFYYYFV-RLPWLFLNPTTLLFLLISF-VYIKPAR 283
Query: 309 VVGIAAYFHVLVMSLQPHKEWRFIIYSNPPITLLGATAAAYLFKNIDNKSVTGVAVRFVX 368
++ F + V S HKEWRFIIYS P A A+ F +R +
Sbjct: 284 LLIYVPLFFIFVYSFLGHKEWRFIIYSIPWFNAASAIGASLCFNASKFGKKIFEILRLMF 343
Query: 369 XXXXXXXXFISIAFSYISTMNYPGGVALENFNEIIKSQNITDAVVHLDVPVCMTGATLFG 428
S Y+ YPGG+AL EI +N VH+DV CMTG T F
Sbjct: 344 FSGIIFGFIGSSFLLYVFQYAYPGGLALTRLYEI---ENHPQVSVHMDVYPCMTGITRFS 400
Query: 429 QLDDSWNITYDKTEDGTLSSK---WPSFNYLIT 458
QL SW YDKTED + S F+YLIT
Sbjct: 401 QL-PSW--YYDKTEDPKMLSNSLFISQFDYLIT 430
>7299245 [MU] KOG2516 Protein involved in dolichol pathway for
N-glycosylation (mannosyltransferase family)
Length = 678
Score = 199 bits (506), Expect = 9e-51
Identities = 137/443 (30%), Positives = 212/443 (46%), Gaps = 35/443 (7%)
Query: 6 DLTTVLTCAVSLHLWYSPYTKVEESFTLQAVHDILTYGVFDVSNYDHVTFAGAVPRSFVG 65
D+ +T A HL Y+P+TKVEESF LQA+HDIL Y + + YDH + G VPR+F+G
Sbjct: 2 DILIFVTAAA--HLVYTPFTKVEESFNLQAMHDIL-YLRNNFTQYDHHDYPGVVPRTFIG 58
Query: 66 PLILSALTYPFNL---TISKDPFRTQMLVRFLIGLTNTVALNRFIVAAKKALTKDQXXXX 122
PL++S ++ PF L T+S + F Q +VR ++ +VA N + ++A+TK
Sbjct: 59 PLVVSIISAPFVLLFETLSINKFWAQYVVRLVLAGAISVAWN----SLRQAVTK------ 108
Query: 123 XXXXXXXXXXXXXXASDLTNWFGLFTATQFHLMFYSSRPLPNFVMXXXXXXXXXXXXXEE 182
++ WF T TQFH MFY +RPLPN + +
Sbjct: 109 ------------IYGVEVRLWFTAITITQFHFMFYMTRPLPN-IFALPIVLFAIAYWLRD 155
Query: 183 NYRWSIILLSFTAIVFRLEIXXXXXXXXXXXFFYEKISLTSIVRTGFIGAGAGIFLSIQV 242
++ II + +VFR E+ K+S+ +++ + ++ V
Sbjct: 156 QHKPFIICSGISILVFRSELALFLGILLVVSLLRRKVSIDGLLKVALPAGVCILAATVLV 215
Query: 243 DSYFWNRLTVPEIEAFIFNVVHGESSKWGTQPFFAYLTGYLPMMFIPPTALLLNHLGFKI 302
DS+FW RL PE E +N V +SS WGT PF Y LP +L+L +G +
Sbjct: 216 DSFFWRRLLWPEGEVLWYNTVLNKSSNWGTSPFLWYFYSALPRAM--GASLVLVPIGVAL 273
Query: 303 GPNSFRVVGIAAYFHVLVMSLQPHKEWRFIIYSNPPITLLGATAAAYLFKNIDNKSVTGV 362
P R + ++A VL+ S+ PHKE RFIIY P + + A A ++ N KS
Sbjct: 274 EPR-IRPLVLSALLFVLLYSILPHKELRFIIYVFPVLNIAAACACQRIWMN-SAKSTWHS 331
Query: 363 AVRFVXXXXXXXXXFISIAFSYISTMNYPGGVALENFNEIIKSQNITDAVVHLDVPVCMT 422
+ FI++ IS NYPGG AL + + ++ VH+ +
Sbjct: 332 FLALACGAHLLLNVFITLFLLVISGTNYPGGAALSRLHRL--EAGTSNVSVHISNLAAQS 389
Query: 423 GATLFGQLDDSWNITYDKTEDGT 445
G + F ++++ W + D+T + T
Sbjct: 390 GVSRFMEINNEWTYSKDETMNYT 412
>Hs13129114 [MU] KOG2516 Protein involved in dolichol pathway for
N-glycosylation (mannosyltransferase family)
Length = 488
Score = 197 bits (502), Expect = 3e-50
Identities = 140/446 (31%), Positives = 208/446 (46%), Gaps = 48/446 (10%)
Query: 7 LTTVLTCAVSLHLWYSPYTKVEESFTLQAVHDILTYGVFDVSNYDHVTFAGAVPRSFVGP 66
L +L ++HL PYTKVEESF LQA HD+L Y D+ YDH+ F G VPR+F+GP
Sbjct: 13 LLGLLVAVATVHLVICPYTKVEESFNLQATHDLL-YHWQDLEQYDHLEFPGVVPRTFLGP 71
Query: 67 LILSALTYPFNLTIS---KDPFRTQMLVRFLIGLTNTVALNRFIVAAKKALTKDQXXXXX 123
++++ + P +S F +Q++VR ++GL ++ L K+
Sbjct: 72 VVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLG--------VIFGLWTLQKE------ 117
Query: 124 XXXXXXXXXXXXXASDLTNWFGLFTATQFHLMFYSSRPLPNFVMXXXXXXXXXXXXXEEN 183
+ + F TA QFHLMFY +R LPN + E
Sbjct: 118 --------VRRHFGAMVATMFCWVTAMQFHLMFYCTRTLPNVLALPVVLLALAAWLRHEW 169
Query: 184 YRWSIILLSFTAIVFRLEIXXXXXXXXXXXFFYEKISLTSIVRTGFIGAGAGIFLSIQVD 243
R+ I L +F IVFR+E+ K+S+ +R + L++ VD
Sbjct: 170 ARF-IWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRALRHAVPAGILCLGLTVAVD 228
Query: 244 SYFWNRLTVPEIEAFIFNVVHGESSKWGTQPFFAYLTGYLP------MMFIPPTALLLNH 297
SYFW +LT PE + +N V +SS WGT P Y LP ++FIP L
Sbjct: 229 SYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIP-----LGL 283
Query: 298 LGFKIGPNSFRVVGIAAYFHVLVMSLQPHKEWRFIIYSNPPITLLGATAAAYLFKNIDNK 357
+ + + +G A + SL PHKE RFIIY+ P + + A +YL N
Sbjct: 284 VDRRTHAPTVLALGFMALY-----SLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKS 338
Query: 358 SVTGVAVRFVXXXXXXXXXFISIAFSYISTMNYPGGVALENFNEIIKSQNITDAVVHLDV 417
+ V + + A Y+S NYPGGVA++ ++++ Q TD ++H+DV
Sbjct: 339 WLYKAGSLLVIGHLVVNAAYSATAL-YVSHFNYPGGVAMQRLHQLVPPQ--TDVLLHIDV 395
Query: 418 PVCMTGATLFGQLDDSWNITYDKTED 443
TG + F Q++ +W YDK ED
Sbjct: 396 AAAQTGVSRFLQVNSAWR--YDKRED 419
>CE29152 [MU] KOG2516 Protein involved in dolichol pathway for
N-glycosylation (mannosyltransferase family)
Length = 492
Score = 165 bits (418), Expect = 1e-40
Identities = 128/437 (29%), Positives = 194/437 (44%), Gaps = 43/437 (9%)
Query: 17 LHLWYSPYTKVEESFTLQAVHDILTYGVFDVSNYDHVTFAGAVPRSFVGPLILSALTYPF 76
+H+ +P TKVEESF +QA HD++ + ++SNYDH F G VPR+F+GP+ L+ L+ P
Sbjct: 14 IHIILAPGTKVEESFNVQATHDLMFHLPTNLSNYDHSQFPGVVPRTFIGPISLAILSSPM 73
Query: 77 NLTI---SKDPFRTQMLVRFLIGLTNTVALNRFIVAAKKALTKDQXXXXXXXXXXXXXXX 133
+ + +L+R +GL N +A F + + ++
Sbjct: 74 SFIFRFWAIPKMWQLLLIRATLGLMNAMAFLYFARSVNRKFGRETAM------------- 120
Query: 134 XXXASDLTNWFGLFTATQFHLMFYSSRPLPNFVMXXXXXXXXXXXXXEENYRWSIILLSF 193
+ L TQFH +FY SRPLPN E Y ++ +
Sbjct: 121 ---------YLRLIMCTQFHYIFYMSRPLPN-TFALILVMIVFERLLEGRYESAVRYATA 170
Query: 194 TAIVFRLEIXXXXXXXXXXXFFYEKISL---TSIVRTGFIGAGAGIFLSIQVDSYFWNRL 250
+ I+FR E+ ++ + + G A + +SI +DSYFW R
Sbjct: 171 SVILFRCELVLLYGPIFLGYMISGRLKVFGFDGAIAIGVRIAAMCLAVSIPIDSYFWGRP 230
Query: 251 TVPEIEAFIFNVVHGESSKWGTQPFFAYLTGYLPMMFIPPTALLLNHLGFKIGPNSFRVV 310
PE E FNVV S ++GTQPF Y LP + T LL LG + ++V
Sbjct: 231 LWPEGEVMFFNVVENRSHEYGTQPFLWYFYSALPRCLL--TTTLLVPLGLLVDRRLPQIV 288
Query: 311 GIAAYFHVLVMSLQPHKEWRFIIYSNPPITLLGATAAAYLFKNIDNKSVTGVAVRFVXXX 370
+ + + + S PHKE RFIIY P L A A + N +KS + + F
Sbjct: 289 -LPSVIFIFLYSFLPHKELRFIIYVLPIFCLSAAVFCARMLIN-RHKSFFRMILFFGVIL 346
Query: 371 XXXXXXFISIAFSYISTMNYPGGVALENFNEIIKSQNITDA----VVHLDVPVCMTGATL 426
+ F +++ NYPG AL ++ QN DA V++D TG
Sbjct: 347 HLLANVLCTGMFLLVASKNYPGFDAL----NYLQFQNRFDAKKPVTVYIDNACAQTGVNR 402
Query: 427 FGQLDDSWNITYDKTED 443
F ++D+W TY+KTE+
Sbjct: 403 FLHINDAW--TYNKTEN 417
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.325 0.140 0.429
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 28,136,289
Number of Sequences: 60738
Number of extensions: 1088541
Number of successful extensions: 2639
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2605
Number of HSP's gapped (non-prelim): 7
length of query: 541
length of database: 30,389,216
effective HSP length: 111
effective length of query: 430
effective length of database: 23,647,298
effective search space: 10168338140
effective search space used: 10168338140
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)