ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2126 good H KOG2743 Coenzyme transport and metabolism Cobalamin synthesis protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2126 754098 755363 422
(422 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YNR029c [H] KOG2743 Cobalamin synthesis protein 459 e-129
Hs13236499 [H] KOG2743 Cobalamin synthesis protein 246 6e-65
Hs20532699 [H] KOG2743 Cobalamin synthesis protein 244 1e-64
Hs20481017 [H] KOG2743 Cobalamin synthesis protein 238 2e-62
SPBC15D4.05 [H] KOG2743 Cobalamin synthesis protein 185 1e-46
At1g26520 [H] KOG2743 Cobalamin synthesis protein 156 6e-38
At1g80480 [H] KOG2743 Cobalamin synthesis protein 153 4e-37
At1g15730 [H] KOG2743 Cobalamin synthesis protein 152 1e-36
>YNR029c [H] KOG2743 Cobalamin synthesis protein
Length = 429
Score = 459 bits (1181), Expect = e-129
Identities = 246/432 (56%), Positives = 311/432 (71%), Gaps = 18/432 (4%)
Query: 1 MSALKDFSFKEDEDGELPVLVTGNEPDLKKILDTYKPDGGKHIVSDKKIALANRQLDE-- 58
MSAL++ F E+EDGELP LVTG E +L++IL+ DGG +IVSD K+ N+Q++
Sbjct: 1 MSALRNIKFNEEEDGELPCLVTGEENNLQEILENVSYDGG-NIVSDAKVERVNKQVENTS 59
Query: 59 ----DFDRQQRIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITI 114
D ++RIPV+IITGYLG+GKSTLLE+IALKG++K++AVILNEFGDSSEIEK++TI
Sbjct: 60 AGATDVHEKKRIPVSIITGYLGSGKSTLLEKIALKGADKKIAVILNEFGDSSEIEKAMTI 119
Query: 115 KNKGEVYEEWLDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKM 174
KN Y+EWLDLGNGCLCCSLK++GVKAIEDMV RSPGKIDYILLETSGIADP PIAKM
Sbjct: 120 KNGSNSYQEWLDLGNGCLCCSLKNIGVKAIEDMVERSPGKIDYILLETSGIADPAPIAKM 179
Query: 175 FWQDKGLNSNVYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAM 234
FWQD+GLNS+VYIDGIITVLD EHI+ CLDDIS AHWHG+KV + E TIAH Q+AM
Sbjct: 180 FWQDEGLNSSVYIDGIITVLDCEHILKCLDDISIDAHWHGDKVGL--EGNLTIAHFQLAM 237
Query: 235 ADAILVNKSDKIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFESTKFL 294
AD I++NK D IE L+ VR IN++AP+++TK+ D + +LD++A++S +
Sbjct: 238 ADRIIMNKYDTIEHSPEMVKQLKERVREINSIAPMFFTKYSDTPIQNLLDIHAYDSVRIS 297
Query: 295 ----QKDYTTTFHDHRISTITLAF-PFFQTEDQLDTFIKDFLQVLLWKDFGVDTGSGLQS 349
T HD R+ TI L F P E+ + FIK FLQ LLWK+FG T G
Sbjct: 298 DILDSGSGNGTIHDDRMGTIMLTFRPLKNEEEYNNKFIKQFLQPLLWKNFGAMTVLG-GR 356
Query: 350 AHHENPDFEVHRTKGVIMVGNE---CKVIQGVRDTYDIIPGEKLPNITENKIVLIGKHLD 406
+ D+EV RTKG+I++ E +VIQGVRDTYD+ PG+ + E KIVLIGK+L+
Sbjct: 357 RRDDGRDWEVQRTKGLILIEGENPIARVIQGVRDTYDVFPGKYDGSNKECKIVLIGKYLE 416
Query: 407 ENYIRHLLNKAI 418
+ I LL K +
Sbjct: 417 KESIEELLRKTL 428
>Hs13236499 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 246 bits (627), Expect = 6e-65
Identities = 153/364 (42%), Positives = 224/364 (61%), Gaps = 47/364 (12%)
Query: 65 RIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITIKNKGEVYEEW 124
+IPVTIITGYLGAGK+TLL I + +KR+AVILNEFG+ S +EKS+ + GE+YEEW
Sbjct: 41 KIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNEFGEGSALEKSLAVSQGGELYEEW 100
Query: 125 LDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNSN 184
L+L NGCLCCS+KD G++AIE+++ + GK DYILLET+G+ADPG +A MFW D L S+
Sbjct: 101 LELRNGCLCCSVKDNGLRAIENLMQKK-GKFDYILLETTGLADPGAVASMFWVDAELGSD 159
Query: 185 VYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKSD 244
+Y+DGIIT++DS++ + L + P + A Q+A+ADAIL+NK+D
Sbjct: 160 IYLDGIITIVDSKYGLKHLTEEKP-------------DGLINEATRQVALADAILINKTD 206
Query: 245 KIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFES------TKFLQKDY 298
+ ++ KK L +RSIN + I T+ ++L VLDL+AF+S K LQ
Sbjct: 207 LVPEEDVKK--LRTTIRSINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP 264
Query: 299 TTTFH-DHRISTITLAFPFFQTEDQLDTFIKDFLQVLLWKDFGVDTGSGLQSAHHENPDF 357
T H D I TIT P E+ L+ FI Q LLW+ + +N
Sbjct: 265 GTQPHLDQSIVTITFEVPGNAKEEHLNMFI----QNLLWEK---------NVRNKDNHCM 311
Query: 358 EVHRTKGVIMVGNECK--VIQGVRDTYDIIPGEKLP----NITE--NKIVLIGKHLDENY 409
EV R KG++ + ++ + ++QGV + YD+ E+ P + TE N++VL+G++LD++
Sbjct: 312 EVIRLKGLVSIKDKSQQVIVQGVHELYDL---EETPVSWKDDTERTNRLVLLGRNLDKDI 368
Query: 410 IRHL 413
++ L
Sbjct: 369 LKQL 372
>Hs20532699 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 244 bits (624), Expect = 1e-64
Identities = 153/364 (42%), Positives = 224/364 (61%), Gaps = 47/364 (12%)
Query: 65 RIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITIKNKGEVYEEW 124
+IPVTIITGYLGAGK+TLL I + +KR+AVILNEFG+ S +EKS+ + GE+YEEW
Sbjct: 41 KIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNEFGEGSALEKSLAVSQGGELYEEW 100
Query: 125 LDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNSN 184
L+L NGCLCCS+KD G++AIE+++ + GK DYILLET+G+ADPG +A MFW D L S+
Sbjct: 101 LELRNGCLCCSVKDNGLRAIENLMQKK-GKFDYILLETTGLADPGAVASMFWVDAELGSD 159
Query: 185 VYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKSD 244
+Y+DGIIT++DS++ + L + P + A Q+A+ADAIL+NK+D
Sbjct: 160 IYLDGIITIVDSKYGLKHLAEEKP-------------DGLINEATRQVALADAILINKTD 206
Query: 245 KIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFES------TKFLQKDY 298
+ ++ KK L +RSIN + I T+ ++L VLDL+AF+S K LQ
Sbjct: 207 LVPEEDVKK--LRATIRSINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP 264
Query: 299 TTTFH-DHRISTITLAFPFFQTEDQLDTFIKDFLQVLLWKDFGVDTGSGLQSAHHENPDF 357
T H D I TIT P E+ L+ FI Q LLW+ + +N
Sbjct: 265 GTQPHLDQSIVTITFEVPGNAKEEHLNMFI----QNLLWEK---------NVRNKDNHCM 311
Query: 358 EVHRTKGVIMVGNECK--VIQGVRDTYDIIPGEKLP----NITE--NKIVLIGKHLDENY 409
EV R KG++ + ++ + ++QGV + YD+ E+ P + TE N++VL+G++LD++
Sbjct: 312 EVIRLKGLVSIKDKSQQVIVQGVHELYDL---EETPVSWKDDTERTNRLVLLGRNLDKDI 368
Query: 410 IRHL 413
++ L
Sbjct: 369 LKQL 372
>Hs20481017 [H] KOG2743 Cobalamin synthesis protein
Length = 395
Score = 238 bits (606), Expect = 2e-62
Identities = 151/364 (41%), Positives = 221/364 (60%), Gaps = 47/364 (12%)
Query: 65 RIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITIKNKGEVYEEW 124
+IPVTIITGYLGAGK+TLL I + +KR+AVILNE G+ S +EKS+ + GE+YEEW
Sbjct: 41 KIPVTIITGYLGAGKTTLLNYILTEQHSKRVAVILNESGEGSALEKSLAVSQGGELYEEW 100
Query: 125 LDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNSN 184
L+L NGCLCCS+KD G++AIE+++ + GK D ILLET+G+ADPG +A MFW D L S+
Sbjct: 101 LELRNGCLCCSVKDNGLRAIENLMQKK-GKFDDILLETTGLADPGAVASMFWVDAELGSD 159
Query: 185 VYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKSD 244
+Y+DGIIT++DS++ + L + P + A Q+A+AD IL+NK+D
Sbjct: 160 IYLDGIITIVDSKYGLKHLTEEKP-------------DGLINEATRQVALADIILINKTD 206
Query: 245 KIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFES------TKFLQKDY 298
+ ++ KK L +RSIN + I T+ ++L VLDL+AF+S K LQ
Sbjct: 207 LVPEEDVKK--LRTTIRSINGLGQILETQRSRVDLSNVLDLHAFDSLSGISLQKKLQHVP 264
Query: 299 TTTFH-DHRISTITLAFPFFQTEDQLDTFIKDFLQVLLWKDFGVDTGSGLQSAHHENPDF 357
T H D I TIT P E+ L+ FI Q LLW+ + +N
Sbjct: 265 GTQPHLDQSIVTITFEVPGNAKEEHLNMFI----QNLLWEK---------NVRNKDNHCM 311
Query: 358 EVHRTKGVIMVGNECK--VIQGVRDTYDIIPGEKLP----NITE--NKIVLIGKHLDENY 409
EV R KG++ + ++ + ++QGV + YD+ E+ P + TE N++VLIG++LD++
Sbjct: 312 EVIRLKGLVSIKDKSQQVIVQGVHELYDL---EETPVSWKDDTERTNRLVLIGRNLDKDI 368
Query: 410 IRHL 413
++ L
Sbjct: 369 LKQL 372
>SPBC15D4.05 [H] KOG2743 Cobalamin synthesis protein
Length = 411
Score = 185 bits (469), Expect = 1e-46
Identities = 136/433 (31%), Positives = 228/433 (52%), Gaps = 71/433 (16%)
Query: 9 FKEDEDGELPVLVTGNEPDLKKILDTYKPDGGKHIVSDKKIALANRQLD---EDFDRQ-Q 64
F+ + + E+P LV E + + +T + S+ I +Q D D D
Sbjct: 3 FEIENESEIPQLVDVEEVNTQPHSETLSVPT---VSSNNDIDSFEQQSDFLLVDHDSTLD 59
Query: 65 RIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITIKNKGE-VYEE 123
+PVTI+TG+LGAGK++LL I + KR+AV++NE GDS ++E+S+ GE +YEE
Sbjct: 60 PVPVTILTGFLGAGKTSLLRSILENRNGKRVAVLMNEVGDSGDLERSLMEDVGGEELYEE 119
Query: 124 WLDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNS 183
W+ L NGC+CC++KD G+KA+E ++ R G+ D I++ET+GIA+PGP+A+ FW D L S
Sbjct: 120 WVALSNGCMCCTVKDNGIKALEKIM-RQKGRFDNIVIETTGIANPGPLAQTFWLDDALKS 178
Query: 184 NVYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKS 243
+V +DGI+TV+D ++I L D ++ I +QI+ AD +++NK+
Sbjct: 179 DVKLDGIVTVIDCKNIDNILKD------------------ESDIGFIQISHADCLILNKT 220
Query: 244 DKIEGDESKKYDLENVVRSINTVAPIYYTKFGDI-NLDLVLDLNAF--ESTKFL------ 294
D I + + + IN +A I T +G + ++ +LDL+A+ E+T L
Sbjct: 221 DLISSEALSV--VRQTILKINCLAKIIETTYGRLDDISEILDLDAYGNENTSNLEWSIQR 278
Query: 295 ----------------QKDYTTTFHDHRISTITLAFPFFQTEDQLDTFIKDFLQVLLWKD 338
Q ++ H IST T P +++ F++ +++ LW
Sbjct: 279 SNDSNINCSTCLDVDCQHLHSLDTHSLDISTHTFKLPPLMSKEVFQQFLQ-WVRHTLW-- 335
Query: 339 FGVDTGSGLQSAHHENPDFEVHRTKGVIMVGN-ECKVIQGVRDTYDIIPGEKLPNITENK 397
S L+ E+ +F ++R+KG+ + + QGVR+ ++I+P + P K
Sbjct: 336 ------SCLEEG--EDEEFMIYRSKGIFNKDDGSWYIFQGVREVFEIMPLSEKPRAFLEK 387
Query: 398 -----IVLIGKHL 405
I+LIG++L
Sbjct: 388 DIHPEIILIGRNL 400
>At1g26520 [H] KOG2743 Cobalamin synthesis protein
Length = 301
Score = 156 bits (394), Expect = 6e-38
Identities = 90/227 (39%), Positives = 141/227 (61%), Gaps = 17/227 (7%)
Query: 66 IPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKS-ITIKNKGEVYEEW 124
+ V++ITGYLGAGKSTL+ I KR+AVILNEFG+ +E++ I +G + EEW
Sbjct: 42 VGVSVITGYLGAGKSTLVNYILNGKHGKRIAVILNEFGEEIGVERAMINEGEEGAIVEEW 101
Query: 125 LDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNSN 184
++L NGC+CC++K V+A+E +V R ++D+ILLET+G+A+P P+A + W D L S
Sbjct: 102 VELANGCVCCTVKHSLVQALEQLVQRK-DRLDHILLETTGLANPAPLASILWLDDQLESE 160
Query: 185 VYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKSD 244
V +D I+TV+D++++ L++ D S A QIA AD I++NK D
Sbjct: 161 VKLDCIVTVVDAKNLRFQLNERR-------------DSSSFPEAFNQIAFADTIIMNKVD 207
Query: 245 KIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFEST 291
I +ES +LE + SIN++A + + ++L +L+ A++ST
Sbjct: 208 LISQEESD--ELEKEIHSINSLANVIRSVRCQVDLSNILNCQAYDST 252
>At1g80480 [H] KOG2743 Cobalamin synthesis protein
Length = 444
Score = 153 bits (387), Expect = 4e-37
Identities = 88/244 (36%), Positives = 150/244 (61%), Gaps = 20/244 (8%)
Query: 65 RIPVTIITGYLGAGKSTLLEQIALKGSNKRLAVILNEFGDSSEIEKSITIKNKGEVYEEW 124
RIP TIITG+LG+GK+TLL I + KR+AVI NEFG+ +I+ S+ + +K E+
Sbjct: 88 RIPATIITGFLGSGKTTLLNHILTRDHGKRIAVIENEFGEV-DIDGSL-VASKSIGAEDI 145
Query: 125 LDLGNGCLCCSLKDVGVKAIEDMVARSPGKIDYILLETSGIADPGPIAKMFWQDKGLNSN 184
+ L NGCLCC+++ V+ I ++V GK D+I++ET+G+A+P PI + F+ ++ + ++
Sbjct: 146 VMLNNGCLCCTVRGDLVRMIGELVNTKKGKFDHIVIETTGLANPAPIIQTFYAEEEIFND 205
Query: 185 VYIDGIITVLDSEHIITCLDDISPTAHWHGEKVTVDDESKTTIAHLQIAMADAILVNKSD 244
V +DG++T++D++H LD++ P E A QIA AD I+VNK+D
Sbjct: 206 VKLDGVVTLVDAKHARLHLDEVKP-------------EGVVNEAVEQIAYADRIIVNKTD 252
Query: 245 KIEGDESKKYDLENVVRSINTVAPIYYTKFGDINLDLVLDLNAFESTKF---LQKDYTTT 301
+ E++ + +++IN++A + TK+G+++LD VL + F+ + + +D
Sbjct: 253 LV--GEAELGSVVQRIKTINSMAQMTRTKYGNVDLDYVLGIGGFDLERIESSVNEDDKGD 310
Query: 302 FHDH 305
HDH
Sbjct: 311 HHDH 314
>At1g15730 [H] KOG2743 Cobalamin synthesis protein
Length = 448
Score = 152 bits (383), Expect = 1e-36
Identities = 117/391 (29%), Positives = 193/391 (48%), Gaps = 47/391 (12%)
Query: 43 IVSDKKIALANRQLDED-FD------RQQRIPVTIITGYLGAGKSTLLEQIALKGSNKRL 95
+ SD +A+ + DED FD RIP TIITG+LG+GK+TLL I KR+
Sbjct: 65 VASDSSLAVVD---DEDIFDVASEILPDNRIPATIITGFLGSGKTTLLNHILTGDHGKRI 121
Query: 96 AVILNEFGDSSEIEKSITIKNKGEVYEEWLDLGNGCLCCSLKDVGVKAIEDMVARSPGKI 155
AVI NEFG+ + + G E+ + L NGCLCC+++ V+ I +MV G+
Sbjct: 122 AVIENEFGEVDIDGSLVAAQTAGA--EDIMMLNNGCLCCTVRGDLVRMISEMVQTKKGRF 179
Query: 156 DYILLETSGIADPGPIAKMFWQDKGLNSNVYIDGIITVLDSEHIITCLDDISPTAHWHGE 215
D+I++ET+G+A+P PI + F+ + + ++V +DG++T++D++H LD++ P
Sbjct: 180 DHIVIETTGLANPAPIIQTFYAEDEIFNDVKLDGVVTLVDAKHARLHLDEVKP------- 232
Query: 216 KVTVDDESKTTIAHLQIAMADAILVNKSDKIEGDESKKYDLENVVRSINTVAPIYYTKFG 275
E A QIA AD I+VNK+D + E + + +++IN++A + TK+G
Sbjct: 233 ------EGYVNEAVEQIAYADRIIVNKTDLV--GEPELASVMQRIKTINSMAHMKRTKYG 284
Query: 276 DINLDLVL-----DLNAFESTKFLQKDYTTTFHD-----------HRISTITLAFPFFQT 319
++LD VL DL ES+ ++ HD H +
Sbjct: 285 KVDLDYVLGIGGFDLERIESSVNEEEKEDREGHDDHHHGHDCHDHHNEHEHEHEHEHHHS 344
Query: 320 EDQLDTFIKDFLQVLLWKDFGVDTGSGLQSAHHENPDFEVHRTKGVIMVG--NECKVIQG 377
D + ++ D ++ + A +++R KG++ V +E V QG
Sbjct: 345 HDHTHDPGVGSVSIVCEGDLDLEKANMWLGALLYQRSEDIYRMKGILSVQDMDERFVFQG 404
Query: 378 VRDTYDIIPGE--KLPNITENKIVLIGKHLD 406
V + ++ P + NKIV IGK+L+
Sbjct: 405 VHEIFEGSPDRLWRKDETRTNKIVFIGKNLN 435
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.138 0.398
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,503,487
Number of Sequences: 60738
Number of extensions: 1195961
Number of successful extensions: 3240
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3196
Number of HSP's gapped (non-prelim): 8
length of query: 422
length of database: 30,389,216
effective HSP length: 109
effective length of query: 313
effective length of database: 23,768,774
effective search space: 7439626262
effective search space used: 7439626262
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)