ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2137 good UI KOG4540 Intracellular trafficking, secretion, and vesicular transport Putative lipase essential for disintegration of autophagic bodies inside the vacuole
r_klactIII2137 good UI KOG4540 Lipid transport and metabolism Putative lipase essential for disintegration of autophagic bodies inside the vacuole
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2137 760883 759291 -531
(531 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR068w [UI] KOG4540 Putative lipase essential for disintegratio... 530 e-150
SPAC23C4.16c [UI] KOG4540 Putative lipase essential for disinteg... 340 3e-93
ECU04g0660 [UI] KOG4540 Putative lipase essential for disintegra... 83 9e-16
>YCR068w [UI] KOG4540 Putative lipase essential for disintegration of
autophagic bodies inside the vacuole
Length = 520
Score = 530 bits (1366), Expect = e-150
Identities = 246/488 (50%), Positives = 331/488 (67%), Gaps = 22/488 (4%)
Query: 49 SGGDSTKDKTHTFTIKEIHFRPLDEKTQSYGSLQVTPEFVNMAKSEFEQNVAVASGDNED 108
S G + TF +K I+ + + + L+VTPE ++ A +++ +D
Sbjct: 46 SRGAMDQKSDGTFRLKSIYRHGVGANHRLHQRLEVTPEVISAAGMLYQETTT----QGQD 101
Query: 109 LYDKQ-LWSASIK---SNPWTHQFTLKQGSIKMKRMVNRDPDYVESFL---DYAHENPEM 161
D++ LW+ + + +NP+ +F L++ + MKRM RDP+++ES++ Y E E
Sbjct: 102 FEDQEPLWTTNAEYATTNPFDFEFELRRMPLLMKRMKERDPEFIESYIYGETYMTEEEE- 160
Query: 162 ARKVHLDWVDESVLAPNVTDKETVISLALMSSNAYVRLPYEGDWRNLSDWNNDLNPDLSV 221
H W+D+ ++APN+TD+ TV+SLALMSSNAYVR+P GDWRN+++ N+ P+
Sbjct: 161 ----HAMWIDDDIVAPNITDRGTVVSLALMSSNAYVRIPQTGDWRNVTEPWNETEPE--- 213
Query: 222 GIGWDSDGVRGHIFSNDDSSVIVIALKGTSAQGLPGSGEDETTDNDKLNDNVLFSCCCAR 281
GWD DG+RGH+F N+ +++V+++KGTSAQGLPGSGEDETT NDK+NDN+LFSCCCAR
Sbjct: 214 DFGWDGDGIRGHVFYNEVENIVVLSIKGTSAQGLPGSGEDETTGNDKINDNLLFSCCCAR 273
Query: 282 VSYLWKTACDCYVKSYTCDEKCLEQELVRKDRYYQAVLDIYRSVVTAHPNSAIWITXXXX 341
VSYLW T CDCYVKSY CDE CLE+EL RKDR+Y AV+DIY+ V+ +P++AIW+T
Sbjct: 274 VSYLWTTVCDCYVKSYICDESCLEKELRRKDRFYSAVVDIYKGVLKEYPDAAIWVTGHSL 333
Query: 342 XXXXXXXXXRTFGAPAVAFEAPGELLATKRLHLPMPPGLPAYQEGVWHIGHTADPIFMGT 401
RTFG PAVAFE+PGELL +KRLHLP PPGLP+Y EG+WH GH ADPIFMGT
Sbjct: 334 GGALASLLGRTFGLPAVAFESPGELLPSKRLHLPFPPGLPSYMEGIWHFGHNADPIFMGT 393
Query: 402 CNGASSSCSIAGYAMETSCHSGKVCVYDVVTDKGWHVNMLNHRIHTVIDGILTDYDTVAK 461
CNGASSSCS+ GYAMET+CH+G+VCVYDVV DKGW VNM NHRIH VID +L Y+ AK
Sbjct: 394 CNGASSSCSLVGYAMETACHTGRVCVYDVVNDKGWSVNMFNHRIHKVIDEVLLGYEQAAK 453
Query: 462 CKTPDACHDCFNWNYVKGRDVPKKHKXXXXXXXXXXXXXXXLTVGPSPPEKTTTSCIGRN 521
C P+ C DC+NW ++ RD + T + ++++C+GRN
Sbjct: 454 CVEPEPCVDCYNWKFIPSRDWESSSRLITKTKSHAAPTTTTRTTATT---TSSSTCVGRN 510
Query: 522 WIGICTEY 529
W+G CT+Y
Sbjct: 511 WLGFCTKY 518
>SPAC23C4.16c [UI] KOG4540 Putative lipase essential for disintegration of
autophagic bodies inside the vacuole
Length = 424
Score = 340 bits (872), Expect = 3e-93
Identities = 151/309 (48%), Positives = 209/309 (66%), Gaps = 9/309 (2%)
Query: 169 WVDESVLAPNVTDKETVISLALMSSNAYVRLPYEGDWRNLS-DWNNDLNPDLSVGIGWDS 227
W E+++ P++TDKET+ SL MS NAY ++P++GDW +L DWN + P G GW+
Sbjct: 119 WEQEAIVIPDITDKETIYSLGKMSYNAYQKIPFDGDWLDLGPDWN--ITPP--EGFGWEE 174
Query: 228 DGVRGHIFSNDDSSVIVIALKGTSAQGLPGSGEDETTDNDKLNDNVLFSCCCARVSYLWK 287
DG+RGH+FSNDD+S I+IA+KGTS G+ G G T+ D++NDN+LFSCCCARVS+ W
Sbjct: 175 DGLRGHVFSNDDNSTIIIAMKGTSIFGI-GRG---TSQKDRVNDNLLFSCCCARVSWAWS 230
Query: 288 TACDCYVKSYTCDEKCLEQELVRKDRYYQAVLDIYRSVVTAHPNSAIWITXXXXXXXXXX 347
T C CY +YTC + CLE E+ RYY A LDI+ SV +P++ IW+T
Sbjct: 231 TVCGCYKNTYTCSQTCLEDEVQDDSRYYSASLDIFYSVKELYPDAQIWLTGHSLGGATAA 290
Query: 348 XXXRTFGAPAVAFEAPGELLATKRLHLPMPPGLPAYQEGVWHIGHTADPIFMGTCNGASS 407
+FG P V FEAPG+ +A +RLHLPMPPGLP + VWH GH ADPI++G C G +S
Sbjct: 291 LMGLSFGIPTVTFEAPGDRMAARRLHLPMPPGLPDEESLVWHFGHNADPIYLGKCTGPTS 350
Query: 408 SCSIAGYAMETSCHSGKVCVYDVVTDKGWHVNMLNHRIHTVIDGILTDYDTVAKCKTPDA 467
C GYAME++CH+G+ C+YDVV DKGWH+++ +HR+ TV++ ++ Y+ + C
Sbjct: 351 LCWAGGYAMESTCHTGQECLYDVVKDKGWHLSITHHRMQTVLNDVIDAYEKLPDCSHTPN 410
Query: 468 CHDCFNWNY 476
C DC+ W +
Sbjct: 411 CVDCYLWEF 419
>ECU04g0660 [UI] KOG4540 Putative lipase essential for disintegration of
autophagic bodies inside the vacuole
Length = 295
Score = 83.2 bits (204), Expect = 9e-16
Identities = 65/233 (27%), Positives = 102/233 (42%), Gaps = 44/233 (18%)
Query: 212 NNDLNPDLSVGIGWDSDGVRGHIFSNDDSSVIVIALKGTSAQGLPGSGEDETTDNDKLND 271
++DL+ L+ G+D DG+R +F D IV+A KGT+ + G T+ DKL D
Sbjct: 65 SHDLDTVLAT-FGYDGDGLRAKVFGYDGK--IVVAFKGTTLS-IMGLEIGRTSRKDKLLD 120
Query: 272 NVLFSCCCARVSYLWKTACDCYVKSYTCDEKCLEQELVRKDR--YYQAVLDIYRSVVTAH 329
+L+S C D++C E+++ D Y+ L+I ++ +
Sbjct: 121 RILYSICK--------------------DKECEEEKIREFDSIGYFSDALEILGAIRRIY 160
Query: 330 PNSAIWITXXXXXXXXXXXXXRTFGAPAVAFEAPGELLATKRLHLPMPPGLPAYQEG--- 386
+ +T + P VAF +PG+ A L L Y +G
Sbjct: 161 HEGRLVLTGHSLGGTIASLLGIRYNLPVVAFSSPGDAYAANILGL--------YDQGKCY 212
Query: 387 --VWHIGHTADPIFMGTCNGASSSCSIAGYAMETSCHSGK-VCVYDVVTDKGW 436
+ HIG +D +F G C S C I GY +ET CH+G+ +C + D GW
Sbjct: 213 DNIIHIGMCSDVVFRGECTKLYSPCGILGYNIETRCHTGRSLC----IRDGGW 261
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.133 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 32,751,405
Number of Sequences: 60738
Number of extensions: 1394809
Number of successful extensions: 3272
Number of sequences better than 1.0e-05: 3
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3261
Number of HSP's gapped (non-prelim): 3
length of query: 531
length of database: 30,389,216
effective HSP length: 111
effective length of query: 420
effective length of database: 23,647,298
effective search space: 9931865160
effective search space used: 9931865160
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)