ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2208 good S KOG3299 Function unknown Uncharacterized conserved protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2208 782424 783236 271
(271 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR059c [S] KOG3299 Uncharacterized conserved protein 300 1e-81
SPAC27E2.02 [S] KOG3299 Uncharacterized conserved protein 168 7e-42
CE20291 [S] KOG3299 Uncharacterized conserved protein 123 3e-28
Hs8923819 [S] KOG3299 Uncharacterized conserved protein 118 1e-26
YDL177c [S] KOG3299 Uncharacterized conserved protein 65 8e-11
SPBC14C8.09c [S] KOG3299 Uncharacterized conserved protein 65 8e-11
>YCR059c [S] KOG3299 Uncharacterized conserved protein
Length = 258
Score = 300 bits (769), Expect = 1e-81
Identities = 149/259 (57%), Positives = 186/259 (71%), Gaps = 12/259 (4%)
Query: 4 NDDVNDEIDAINAIYPDLLTVVNNDR--IKIKVPQHEDFTLHLSFPEHYPETEPPHLLEV 61
++ + +E++A+ AIYPDLL+ D I +KVPQHE TL +SFP HYP E P+++EV
Sbjct: 5 HEQLVEELEAVEAIYPDLLSKKQEDGSIIVVKVPQHEYMTLQISFPTHYPSEEAPNVIEV 64
Query: 62 SLSAKISA--LYDVKYLNHLFTEVLESVFNPGLVCIFDFLTELDGVLYTEEGEEASGSDE 119
+ ++ LYD KYL HLF EV++SVF+ G VC+FDFLTELDGVLY E EE +
Sbjct: 65 GVCTSLAKRDLYDTKYLQHLFQEVMDSVFHRGSVCLFDFLTELDGVLYVEPEEETEPVQQ 124
Query: 120 VDISDKFENLKIDPFEGWYVSDPITDRKSTFIAFATKANSEEEAFDKLELLRTDNKIARA 179
DI DPFEGW SDPITDR STF+AFA SEE+AF L+LL+TD+K+ +A
Sbjct: 125 SDIPT-------DPFEGWTASDPITDRGSTFMAFAAHVTSEEQAFAMLDLLKTDSKMRKA 177
Query: 180 NHLMVAWRIKGE-NGVSFQDCDDDGETAAGGRMLHLITVMDAWNVIVAVGRWFGGVHIGP 238
NH+M AWRIK + + ++QD DDDGETAAG RMLHLIT+MD WNVIV V RWFGG HIGP
Sbjct: 178 NHVMSAWRIKQDGSAATYQDSDDDGETAAGSRMLHLITIMDVWNVIVVVARWFGGAHIGP 237
Query: 239 DRFKHINSTTREVILKGHF 257
DRFKHINST RE +++ F
Sbjct: 238 DRFKHINSTAREAVVRAGF 256
>SPAC27E2.02 [S] KOG3299 Uncharacterized conserved protein
Length = 280
Score = 168 bits (426), Expect = 7e-42
Identities = 110/280 (39%), Positives = 152/280 (54%), Gaps = 40/280 (14%)
Query: 4 NDDVNDEIDAINAIYPDLLTVVNNDRIK--IKVPQHEDFTLHLSFPEHYPETEPPHLLEV 61
N++ DE+ A+ +IYP L ++ + +P L++ FP YP + P L
Sbjct: 4 NEEFQDELLALESIYPSCLLPISEQSFTYTLSIPD-SSVRLNIQFPLDYPNSAPTVLDAY 62
Query: 62 SLSAKISALYDVKYLNHLFTEVLESVFNPGLVCIFDFLTELDGVLYTEEGEEASGSDEVD 121
+ ++ +VL SV G VCIF ++ +L L + E+A+ E
Sbjct: 63 GIDKTLAE------------DVLLSVAT-GDVCIFSYM-DLLKELVDIDAEQAAAERESK 108
Query: 122 ISD-----------------KFENLKIDPFE---GWYVSDPITDRKSTFIAFATKANSEE 161
+ + K ++ +P++ W S+PITDRKSTF+A AT+ S E
Sbjct: 109 LQEESDKETPVMLNKSHYVAKTPEIQDEPWKPKFDWKESEPITDRKSTFMAHATRVYSTE 168
Query: 162 EAFDKLELLRTDNKIARANHLMVAWRIKGENGVSFQDCDDDGETAAGGRMLHLITVMDAW 221
E + LE L D K+A+ANH MVA+RI NG QD DDDGE+AAG RM HL+T+M A
Sbjct: 169 EVREALEDLYMDKKVAKANHNMVAYRIISPNGNVIQDNDDDGESAAGSRMSHLLTMMSAE 228
Query: 222 NVIVAVGRWFGGVHIGPDRFKHINSTTREVILKGHFTEAA 261
NV V V RWFGGVHIGPDRFKHINS+ RE +L T+AA
Sbjct: 229 NVFVCVSRWFGGVHIGPDRFKHINSSAREAVL---LTDAA 265
>CE20291 [S] KOG3299 Uncharacterized conserved protein
Length = 257
Score = 123 bits (308), Expect = 3e-28
Identities = 81/258 (31%), Positives = 135/258 (51%), Gaps = 25/258 (9%)
Query: 10 EIDAINAIYPDLLTVVNNDRIKIKVPQHEDFTLHLSFPEHYPETEPPHLLEVSLSAKISA 69
EI+++ +I+PDLL + ++ K + T++L PE YP PP ++S
Sbjct: 10 EIESLASIFPDLLEETTDKQLVFKFDRDIGMTINL--PEDYPSISPPIF-------ELSG 60
Query: 70 LYDVKYLNHLFTEVLESVF--NPGLVCIFDFLTELDGVLYTEEGEEASGSDEVD-----I 122
Y + + L V+ N G IF++++ + + E A S D +
Sbjct: 61 PYLRREQKEVLHNSLNDVYIENIGFPVIFNWISLVQDFIRDLPEEVAEPSHVADDVVTPM 120
Query: 123 SDKFENLKIDPFEGWYVSDPITDRKSTFIAFATKANSEEEAFDKLELLRTDNKIARANHL 182
+++ +++ I E +TDRKS F A + S+E+ + +L+++ KI RA H
Sbjct: 121 AEEIDDMNIRHGE------VLTDRKSAFQAHLAEVRSKEDVDRVMRILKSNTKICRATHN 174
Query: 183 MVAWRIKGE-NG--VSFQDCDDDGETAAGGRMLHLITVMDAWNVIVAVGRWFGGVHIGPD 239
+ A+R E NG + DC DDGE A +ML L+ M A NV+V V RW+GG+H+GPD
Sbjct: 175 ITAYRYMTEINGKPIHHHDCVDDGEFGASSKMLELMDRMKADNVMVVVSRWYGGIHLGPD 234
Query: 240 RFKHINSTTREVILKGHF 257
RF+HIN+ TR+++ ++
Sbjct: 235 RFRHINNLTRQILSDNNY 252
>Hs8923819 [S] KOG3299 Uncharacterized conserved protein
Length = 320
Score = 118 bits (295), Expect = 1e-26
Identities = 89/290 (30%), Positives = 143/290 (48%), Gaps = 42/290 (14%)
Query: 8 NDEIDAINAIYPDLLTVVNNDRIKIKVPQHED--------FTLHLSFPEHYPETEPPHL- 58
N+EI+A+ AIY + V++ D KI + D L + P YP T PP
Sbjct: 13 NEEIEAMAAIYGEEWCVID-DCAKIFCIRISDDIDDPKWTLCLQVMLPNEYPGTAPPIYQ 71
Query: 59 -----LEVSLSAKISALYDVKYLNHLFTEVLESVFNPGLVCIFDFLTEL----------- 102
L+ A +S + Y+ ++ ES+ + I D L +
Sbjct: 72 LNAPWLKGQERADLSNSLEEIYIQNIG----ESILYLWVEKIRDVLIQKSQMTEPGPDVK 127
Query: 103 -----------DGVLYTEEGEEASGSDEVDISDKFENLKIDPFEGWYVSDPITDRKSTFI 151
D ++ + E + + + DIS+ ++++ PITDR+STF
Sbjct: 128 KKTEEEDVECEDDLILACQPESSVKALDFDISETRTEVEVEELPPIDHGIPITDRRSTFQ 187
Query: 152 AFATKANSEEEAFDKLELLRTDNKIARANHLMVAWRIKGENGVSF-QDCDDDGETAAGGR 210
A ++ L L + KIA A H + A+RI E+ +F QDC+DDGETAAGGR
Sbjct: 188 AHLAPVVCPKQVKMVLSKLYENKKIASATHNIYAYRIYCEDKQTFLQDCEDDGETAAGGR 247
Query: 211 MLHLITVMDAWNVIVAVGRWFGGVHIGPDRFKHINSTTREVILKGHFTEA 260
+LHL+ +++ NV+V V RW+GG+ +GPDRFKHIN+ R ++++ ++T +
Sbjct: 248 LLHLMEILNVKNVMVVVSRWYGGILLGPDRFKHINNCARNILVEKNYTNS 297
>YDL177c [S] KOG3299 Uncharacterized conserved protein
Length = 170
Score = 65.5 bits (158), Expect = 8e-11
Identities = 47/157 (29%), Positives = 71/157 (44%), Gaps = 36/157 (22%)
Query: 137 WYVSDPITDRKSTFIAFATKANSEEEAFDKLELLRTDNK-IARANHL-MVAWRI------ 188
W S+ + DRKS F A ++++ L+ L +NK +++A+H+ M AWR
Sbjct: 12 WNESEVLVDRKSKFQARCCPLQNQKDIPSILQELTQNNKSVSKASHMHMYAWRTAEVSNN 71
Query: 189 ---------KGENGVSF-------------------QDCDDDGETAAGGRMLHLITVMDA 220
KG Q C D GE AG R+L L+ +
Sbjct: 72 LHLQQEQKKKGNKANKSNNSHVNKSRNITVQPKNIEQGCADCGEAGAGQRLLTLLERANI 131
Query: 221 WNVIVAVGRWFGGVHIGPDRFKHINSTTREVILKGHF 257
+NV+V V RW+GG +G RF+HI++ E + KG F
Sbjct: 132 FNVLVIVTRWYGGTPLGSSRFRHISTCAVETLKKGGF 168
>SPBC14C8.09c [S] KOG3299 Uncharacterized conserved protein
Length = 144
Score = 65.5 bits (158), Expect = 8e-11
Identities = 35/107 (32%), Positives = 60/107 (55%), Gaps = 5/107 (4%)
Query: 138 YVSDPITDRKSTFIAFATKANSEEEAFDKLELLRTDNKIARANHLMVAWRIKGENGVSFQ 197
Y+S+ ITDR+STF+++ + + + + + NH M AWR E +
Sbjct: 29 YMSNLITDRESTFLSYFVPSKDPKMLPVYRFMFQESADLKHCNHKMQAWRFPNE----IE 84
Query: 198 DCDDDGETAAGGRMLHLITVMDAWNVIVAVGRWFGGVHIGPDRFKHI 244
+DDGE +G ++L+++ D + ++V V RW+GG +GP RF+HI
Sbjct: 85 AFNDDGEEYSGQKLLNVLRKEDVYGMVVCV-RWYGGQLLGPVRFQHI 130
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,571,347
Number of Sequences: 60738
Number of extensions: 723519
Number of successful extensions: 1799
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1782
Number of HSP's gapped (non-prelim): 8
length of query: 271
length of database: 30,389,216
effective HSP length: 105
effective length of query: 166
effective length of database: 24,011,726
effective search space: 3985946516
effective search space used: 3985946516
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)