ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2234 good E KOG2616 Amino acid transport and metabolism Pyridoxalphosphate-dependent enzyme/predicted threonine synthase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2234 790816 789287 -510
(510 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YCR053w [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predicte... 819 0.0
SPAC9E9.06c [E] KOG2616 Pyridoxalphosphate-dependent enzyme/pred... 540 e-153
Hs13376251 [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predi... 100 5e-21
Hs8922766 [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predic... 77 8e-14
>YCR053w [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predicted threonine
synthase
Length = 514
Score = 819 bits (2116), Expect = 0.0
Identities = 406/510 (79%), Positives = 450/510 (87%), Gaps = 1/510 (0%)
Query: 2 SQSYRSTRSSDPATKTFEEAIIQGLASDGGLFIPSIIPQVSKSELFDKWSKLSFQDLAFE 61
SQ YRSTRSS P T +FEEAIIQGLA+DGGLFIP IPQV ++ LF+ WSKLSFQDLAF
Sbjct: 5 SQVYRSTRSSSPKTISFEEAIIQGLATDGGLFIPPTIPQVDQATLFNDWSKLSFQDLAFA 64
Query: 62 IMKLYVSSDEIPEADLKDLITRSYSTFRSHDVTPLAKNVTGSNENLHVLELFHGPTYAFK 121
IM+LY++ +EIP+ADLKDLI RSYSTFRS +VTPL +NVTG ENLH+LELFHGPTYAFK
Sbjct: 65 IMRLYIAQEEIPDADLKDLIKRSYSTFRSDEVTPLVQNVTGDKENLHILELFHGPTYAFK 124
Query: 122 DVALQFVGNLFEYFLQRHNDGLPADQRKKITVVGATSGDTGSAAIYGLRGKKDVAVFILY 181
DVALQFVGNLFEYFLQR N LP ++K+ITVVGATSGDTGSAAIYGLRGKKDV+VFILY
Sbjct: 125 DVALQFVGNLFEYFLQRTNANLPEGEKKQITVVGATSGDTGSAAIYGLRGKKDVSVFILY 184
Query: 182 PTGRISPIQEEQMTTVPDKNVQTLSVKGTFDNCQDIVKAIFGDKEFNSKHNVGAVNSINW 241
PTGRISPIQEEQMTTVPD+NVQTLSV GTFDNCQDIVKAIFGDKEFNSKHNVGAVNSINW
Sbjct: 185 PTGRISPIQEEQMTTVPDENVQTLSVTGTFDNCQDIVKAIFGDKEFNSKHNVGAVNSINW 244
Query: 242 ARILAQITYYFYSYFKATTGKE-EKVKFVVPSGNFGDILAGYYAKKMGLPVEKLVIATNE 300
ARILAQ+TYYFYS+F+AT GK+ +KVKFVVPSGNFGDILAGY+AKKMGLP+EKL IATNE
Sbjct: 245 ARILAQMTYYFYSFFQATNGKDSKKVKFVVPSGNFGDILAGYFAKKMGLPIEKLAIATNE 304
Query: 301 NDILDRFLKNGVYERSDDVAATLSPAMDILISSNFERLLWYLAREYVASGDDLKAGETVN 360
NDILDRFLK+G+YERSD VAATLSPAMDILISSNFERLLWYLAREY+A+GDDLKAGE VN
Sbjct: 305 NDILDRFLKSGLYERSDKVAATLSPAMDILISSNFERLLWYLAREYLANGDDLKAGEIVN 364
Query: 361 NWFQELKSKGKFDVPASLIDGAKKDFDSERVSNEETTATIKQVYEQSVNPKHYIIDPHTA 420
NWFQELK+ GKF V S+I+GA KDF SERVSNEET+ TIK++YE SVNPKHYI+DPHTA
Sbjct: 365 NWFQELKTNGKFQVDKSIIEGASKDFTSERVSNEETSETIKKIYESSVNPKHYILDPHTA 424
Query: 421 VGICATKTQITKDNDNTINYISLSTAHPAKFADAVNEALSSFDGYSFEKDVLPEEXXXXX 480
VG+CAT+ I KDND +I YISLSTAHPAKFADAVN ALS F YSFEKDVLPEE
Sbjct: 425 VGVCATERLIAKDNDKSIQYISLSTAHPAKFADAVNNALSGFSNYSFEKDVLPEELKKLS 484
Query: 481 XXXXXXXXVEKADIELVKQTIEEELVKMEV 510
+E+AD+ELVK IEEEL KM++
Sbjct: 485 TLKKKLKFIERADVELVKNAIEEELAKMKL 514
>SPAC9E9.06c [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predicted threonine
synthase
Length = 514
Score = 540 bits (1391), Expect = e-153
Identities = 290/514 (56%), Positives = 352/514 (68%), Gaps = 22/514 (4%)
Query: 4 SYRSTRSSDPATKTFEEAIIQGLASDGGLFIPSIIPQVSKSELFDKWSKLSFQDLAFEIM 63
SY STR + +FEEA+++GLA+DGGLFIPS IPQ+ S + W SF ++AFE+M
Sbjct: 6 SYLSTRGGS-SNFSFEEAVLKGLANDGGLFIPSEIPQLP-SGWIEAWKDKSFPEIAFEVM 63
Query: 64 KLYVSSDEIPEADLKDLITRSYSTFRSHDVTPLAKNVTGSNENLHVLELFHGPTYAFKDV 123
LY+ EI +LK L+ RSYSTFR + TPL G L+VLELFHGPT+AFKDV
Sbjct: 64 SLYIPRSEISADELKKLVDRSYSTFRHPETTPLKSLKNG----LNVLELFHGPTFAFKDV 119
Query: 124 ALQFVGNLFEYFLQRHNDGLPADQRKKITVVGATSGDTGSAAIYGLRGKKDVAVFILYPT 183
ALQF+GNLFE+FL R N P D+R +TVVGATSGDTGSAAIYGLRGKKDV+VFIL+P
Sbjct: 120 ALQFLGNLFEFFLTRKNGNKPEDERDHLTVVGATSGDTGSAAIYGLRGKKDVSVFILFPN 179
Query: 184 GRISPIQEEQMTTVPDKNVQTLSVKGTFDNCQDIVKAIFGDKEFNSKHNVGAVNSINWAR 243
GR+SPIQE QMTTV D NV ++V G FD+CQD+VK IFGD EFN KH++GAVNSINWAR
Sbjct: 180 GRVSPIQEAQMTTVTDPNVHCITVNGVFDDCQDLVKQIFGDVEFNKKHHIGAVNSINWAR 239
Query: 244 ILAQITYYFYSYFKA-TTGKEEKVKFVVPSGNFGDILAGYYAKKMGLPVEKLVIATNEND 302
IL+QITYY YSY GK + V+F+VP+GNFGDILAGYYAK+MGLP ++LVIATNEND
Sbjct: 240 ILSQITYYLYSYLSVYKQGKADDVRFIVPTGNFGDILAGYYAKRMGLPTKQLVIATNEND 299
Query: 303 ILDRFLKNGVYERSDDVAA----------TLSPAMDILISSNFERLLWYLAREYVA-SGD 351
IL+RF K G YE++D T SPAMDIL+SSNFER LWYLA A +
Sbjct: 300 ILNRFFKTGRYEKADSTQVSPSGPISAKETYSPAMDILVSSNFERYLWYLALATEAPNHT 359
Query: 352 DLKAGETVNNWFQELKSKGKFDVPASLIDGAKKDFDSERVSNEETTATIKQVYEQSVNPK 411
+A E ++ W E K G V +++ A++DF SERVSN+ET IK++YE
Sbjct: 360 PAEASEILSRWMNEFKRDGTVTVRPEVLEAARRDFVSERVSNDETIDAIKKIYESD---- 415
Query: 412 HYIIDPHTAVGICATKTQITKDNDNTINYISLSTAHPAKFADAVNEALSSFDGYSFEKDV 471
HYIIDPHTAVG+ + K D I YI LSTAHPAKF AVN ALSS+ Y+F V
Sbjct: 416 HYIIDPHTAVGVETGLRCLEKTKDQDITYICLSTAHPAKFDKAVNLALSSYSDYNFNTQV 475
Query: 472 LPEEXXXXXXXXXXXXXVEKADIELVKQTIEEEL 505
LP E K +I+++KQ IE L
Sbjct: 476 LPIEFDGLLDEERTCIFSGKPNIDILKQIIEVTL 509
>Hs13376251 [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predicted threonine
synthase
Length = 222
Score = 100 bits (249), Expect = 5e-21
Identities = 64/174 (36%), Positives = 93/174 (52%), Gaps = 18/174 (10%)
Query: 287 MGLPVEKLVIATNENDILDRFLKNGVYE-RSDDVAATLSPAMDILISSNFERLLWYLARE 345
MG+P+ K + A+N+N +L F+K G Y+ R +A T SP++DIL SSN ER L +A
Sbjct: 2 MGIPIRKFICASNQNHVLTDFIKTGHYDLRERKLAQTFSPSIDILKSSNLERHLHLMAN- 60
Query: 346 YVASGDDLKAGETVNNWFQELKSKGKFDVPASLIDGAKKDFDSERVSNEETTATIKQVYE 405
K G+ + F L+S+ F + +L++ ++DF ++ S E A I Y
Sbjct: 61 --------KDGQLMTELFNRLESQHHFQIEKALVEKLQQDFVADWCSEGECLAAINSTY- 111
Query: 406 QSVNPKHYIIDPHTAVGICATKTQITKDNDNTINYISLSTAHPAKFADAVNEAL 459
N YI+DPHTAV K + D T I STAH +KFA A+ +AL
Sbjct: 112 ---NTSGYILDPHTAV----AKVVADRVQDKTCPVIISSTAHYSKFAPAIMQAL 158
>Hs8922766 [E] KOG2616 Pyridoxalphosphate-dependent enzyme/predicted threonine
synthase
Length = 265
Score = 76.6 bits (187), Expect = 8e-14
Identities = 54/170 (31%), Positives = 89/170 (51%), Gaps = 15/170 (8%)
Query: 286 KMGLPVEKLVIATNENDILDRFLKNGVYERSDDVAATLSPAMDILISSNFERLLWYLARE 345
K+GLP+ +LV+A N NDI+ R ++ G + S+ V +TL+ AMDI + N ER+ W L
Sbjct: 55 KIGLPI-RLVVAVNRNDIIHRTVQQGDFSLSEAVKSTLASAMDIQVPYNMERVFWLL--- 110
Query: 346 YVASGDDLKAGETVNNWFQELKSKGKFDVPASLIDGAKKDFDSERVSNEETTATIKQVYE 405
SG D + + F+ +S ++P L + S VS+E T T+ + ++
Sbjct: 111 ---SGSDSQVTRALMEQFERTQS---VNLPKELHSKLSEAVTSVSVSDEAITQTMGRCWD 164
Query: 406 QSVNPKHYIIDPHTAVGICATKTQITKDNDNTINYISLSTAHPAKFADAV 455
++ Y++ PH+AV + QI + +T L+ A AKF +AV
Sbjct: 165 EN----QYLLCPHSAVAVNYHYQQIDRQQPSTPR-CCLAPASAAKFPEAV 209
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.133 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 29,351,702
Number of Sequences: 60738
Number of extensions: 1279828
Number of successful extensions: 3244
Number of sequences better than 1.0e-05: 4
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 3230
Number of HSP's gapped (non-prelim): 5
length of query: 510
length of database: 30,389,216
effective HSP length: 111
effective length of query: 399
effective length of database: 23,647,298
effective search space: 9435271902
effective search space used: 9435271902
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)