ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII2251  suspect: LH   S     KOG2357  Function unknown                                               Uncharacterized conserved protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII2251 795095 793872 -408 
         (408 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YNR021w [S] KOG2357 Uncharacterized conserved protein                 313   4e-85
SPBC2G5.01 [S] KOG2357 Uncharacterized conserved protein               61   3e-09

>YNR021w [S] KOG2357 Uncharacterized conserved protein
          Length = 404

 Score =  313 bits (801), Expect = 4e-85
 Identities = 158/360 (43%), Positives = 242/360 (66%), Gaps = 3/360 (0%)

Query: 2   SAILAPLAKAIDYVNALNKDFYILSVEEQKELGLWGRVTAYNWTFELACIGLMAILFFIS 61
           S+I  PL   ++ VN+LN  +  LS +EQK + +W RV  YNWTFEL  +G++ +++   
Sbjct: 3   SSIFGPLTGFLERVNSLNAPYQALSYDEQKAMTIWQRVKFYNWTFELCALGVLFLVYAFY 62

Query: 62  YYGKTLNNKYADKIFGTLNTFLLTKLSFTKVGFSADGKKMPYIEEQNNTWFTSFATGRST 121
            +G ++N K  ++IF +L++FL   L F++VGF+ +  K+  +E QN TWF+SFATGRS 
Sbjct: 63  KFGNSVNLKRGNQIFQSLHSFLANDLKFSRVGFNINDSKIFTVEHQN-TWFSSFATGRSA 121

Query: 122 IESVIVKAHLTARHNPLALLTQKALALFLPTLVANDIEEFISVTITPNGQFVSTEDAKLT 181
           I+S+ +  HL AR NP ++  +  L  F  +L +  +EEF+ + I PNG  V++E A   
Sbjct: 122 IKSINLNLHLVARSNPFSMCLEYLLGFFFASLKSKQLEEFMEIVIRPNGILVTSESAHPN 181

Query: 182 ANSAETLSKLKFITSIVNKAVMTKARDSNYFLSLTHTAENESLPADYVFMSESNKLNGFI 241
            N+ E L+K +F+TSIVNK  M +AR  NYFLS+ HT+EN+ LP ++V+MS+ N+L+GF+
Sbjct: 182 KNAHEILTKFRFVTSIVNKEFMNQARTENYFLSIAHTSENDKLPNNFVYMSDVNQLSGFM 241

Query: 242 PHYAGEDFKVLLSKSAKFLKFISFTDLPEEKPITDKLWESSQLPRCVIYLSLVTSDKDIE 301
            HY+ + ++V LS++   LK+ISFTDLP   P  DK WESS  P+ +I  ++  ++ +++
Sbjct: 242 FHYS-KPYEV-LSQAGNLLKYISFTDLPVNPPRDDKEWESSIEPKAIIRCAVPQNENELK 299

Query: 302 LLEELITKVVEIYDNVTKDIQTNSSNSFITTDILKKGIQLRKEELGKIVKVMQQVEREMA 361
           LL ++I+ VVEIYD  T+D+   S N FIT DILK+   LR++EL KI K M++ E E+A
Sbjct: 300 LLNQIISLVVEIYDGFTQDLVQQSPNLFITNDILKRTTNLRQQELNKIKKFMKETELELA 359


>SPBC2G5.01 [S] KOG2357 Uncharacterized conserved protein
          Length = 374

 Score = 60.8 bits (146), Expect = 3e-09
 Identities = 60/269 (22%), Positives = 117/269 (43%), Gaps = 22/269 (8%)

Query: 29  EQKELGLWGRVTAYNWTFELACIGLMAILFFISYYGKTLNNKYADKIFGTLNTFLLTKLS 88
           E +  G++  V   ++  E   +    +  F     K  N K A + +G+L +    +  
Sbjct: 34  ELENPGMFKHVDWRDFRLEFVILACFFLYVFSFITQKKKNQKIASRWYGSLQSSF--RQQ 91

Query: 89  FTKVGFSADGKKMPYIEEQNNTWFTSFATGRSTIESVIVKAHLTARHNPLALLTQKALAL 148
           F + G   +   + Y    + T F+S+ TGR  +++V     L  R + LA    + + +
Sbjct: 92  FAQYGPGPNSSPIIY---DSPTEFSSYLTGRLNVKNVYTTLQLFPRQDLLAYSLNQIVEI 148

Query: 149 FLPTLVANDIEEFISVTITPNGQF-VSTEDAKLTANSAETLSKLKFITSIVNKAVMTKAR 207
            L  ++++ +       +    QF ++  D  L A         +F+ +IV+K  M   R
Sbjct: 149 LLGNVMSSVLP------VADRFQFDLTLADQNLKAE--------RFVFAIVHKDCMRILR 194

Query: 208 DSNYFLSLTHTAENESLPADYVFMSESNKLNGFIPHYAGEDFKVLLSKSAKFLKFISFTD 267
           +  Y LS T  + +  LP  +V MSE+N+ +  I  +   +F   +++  + L++   TD
Sbjct: 195 EIRYDLSFTRISSSPYLPETHVLMSENNECSQAI--FEIPEFMSSINECIENLEYFIVTD 252

Query: 268 LPEEKPITDKLWESSQLPRCVIYLSLVTS 296
            P   P T+K + +       I +  +TS
Sbjct: 253 QPSVPPATEKDYVTKPRIEASIRIKKITS 281


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.318    0.133    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,685,001
Number of Sequences: 60738
Number of extensions: 782658
Number of successful extensions: 2405
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2400
Number of HSP's gapped (non-prelim): 2
length of query: 408
length of database: 30,389,216
effective HSP length: 109
effective length of query: 299
effective length of database: 23,768,774
effective search space: 7106863426
effective search space used: 7106863426
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)