ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII2268  suspect: LH   U     KOG3081  Intracellular trafficking, secretion, and vesicular transport  Vesicle coat complex COPI, epsilon subunit

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII2268 799820  800698 293  
         (293 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YIL076w [U] KOG3081 Vesicle coat complex COPI epsilon subunit         256   4e-68
7297124 [U] KOG3081 Vesicle coat complex COPI epsilon subunit          52   1e-06

>YIL076w [U] KOG3081 Vesicle coat complex COPI epsilon subunit
          Length = 359

 Score =  256 bits (653), Expect = 4e-68
 Identities = 136/295 (46%), Positives = 191/295 (64%), Gaps = 7/295 (2%)

Query: 1   MDYFTVKQQFYTGNYEEALNEVSKFNKTEDEALTYYRNRSLIALSQFSEGSADSGSLGPV 60
           MDYF +KQ +YTGN+ + L E+ KF+K  D  L +Y+ ++L+AL Q+      S  LG V
Sbjct: 64  MDYFNIKQNYYTGNFVQCLQEIEKFSKVTDNTLLFYKAKTLLALGQYQSQDPTS-KLGKV 122

Query: 61  FEAYYKFLSKPTGSITALEQTVEKAGRSPFALNLLASALTIKGEFKTALEVAVEGIDSDE 120
            + Y +FL   T +I  LE  ++    SP+ L LLA+A  I G+   +LE  VEGID+DE
Sbjct: 123 LDLYVQFLD--TKNIEELENLLKDKQNSPYELYLLATAQAILGDLDKSLETCVEGIDNDE 180

Query: 121 TRGTPELLLTAIQITLLDNQPTIASTMFENFQALQEQ--SNDDEIILNLAESYINFNQGK 178
             GT ELLL AI++ LL+N  + AST+F+N+    E   S D+E+ILNLAESYI F   K
Sbjct: 181 AEGTTELLLLAIEVALLNNNVSTASTIFDNYTNAIEDTVSGDNEMILNLAESYIKFATNK 240

Query: 179 EITGSNFYFFEELSQTFPSWKTQLGLLNLHLQQSHLPESKAIIDLLESEFYDI--KQEAQ 236
           E   SNFY++EELSQTFP+WKTQLGLLNLHLQQ ++ E++ I++LL S++Y +  K+ A 
Sbjct: 241 ETATSNFYYYEELSQTFPTWKTQLGLLNLHLQQRNIAEAQGIVELLLSDYYSVEQKENAV 300

Query: 237 TYKPDLLANKITYTILSGGNANELRSELQQLKPSHPLCVADLENNKTFDQIVAKY 291
            YKP  LAN+IT  ++ G +  +L ++L +L   H       E +  FD++V KY
Sbjct: 301 LYKPTFLANQITLALMQGLDTEDLTNQLVKLDHEHAFIKHHQEIDAKFDELVRKY 355


>7297124 [U] KOG3081 Vesicle coat complex COPI epsilon subunit
          Length = 306

 Score = 52.0 bits (123), Expect = 1e-06
 Identities = 70/304 (23%), Positives = 129/304 (42%), Gaps = 39/304 (12%)

Query: 4   FTVKQQFYTGNYEEALNEVSKFNKTEDEALTYYRNRSLIAL-------SQFSEG-SADSG 55
           F  + ++Y GN+  ++N V     T    L  Y   S +A+       S   EG S    
Sbjct: 15  FDARNEYYIGNFMGSINFVLPEQGTAGPELLSYMYLSYLAIDSGRIVASDIKEGNSTPLQ 74

Query: 56  SLGPVFEAYYKFLSKPTGSITALEQTVEKAGRSPFALNL--LASALTI--KGEFKTALEV 111
           +L  V EA+     +P+ +   LE+  +K        N+  LA+A+     G+F+ AL++
Sbjct: 75  ALRLVHEAF----EQPSRTEELLEKLTDKVAGEEDETNIWHLATAIVYCHDGQFENALKI 130

Query: 112 AVEGIDSDETRGTPELLLTAIQITLLDNQPTIASTMFENFQALQEQSNDDEIILNLAESY 171
                      G+  L   A+ +  L     +   + +   A  ++ +DD  +  LA+++
Sbjct: 131 ---------LHGSTNLESMALSVQCLLRLQRV--DLAKQLVAKMQEISDDATLTQLAQAW 179

Query: 172 INFNQGKEITGSNFYFFEELSQTF-PSWKTQLGLLNLHLQQSHLPESKAIIDLLESEFYD 230
           +   QG E     F+ ++E  + F P+     G   +HL      E+ ++  L ES    
Sbjct: 180 VALAQGTEQMQDAFHIYQEFCEKFKPTPALLNGQAVVHLGLERYEEADSV--LRES---- 233

Query: 231 IKQEAQTYKPDLLANKITYTILSGGNANELRSELQQLKPSHPLC--VADLENNKT-FDQI 287
           + ++   Y  D L N + +  L+G     +   L+QL+  +P    V DL+     FD++
Sbjct: 234 LLKKHNDY--DTLINLMVHAHLTGKPTEAITRNLEQLRQFYPKSDFVTDLDKKSAEFDRL 291

Query: 288 VAKY 291
             +Y
Sbjct: 292 CLQY 295


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.313    0.131    0.356 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,798,772
Number of Sequences: 60738
Number of extensions: 680513
Number of successful extensions: 1596
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1592
Number of HSP's gapped (non-prelim): 2
length of query: 293
length of database: 30,389,216
effective HSP length: 105
effective length of query: 188
effective length of database: 24,011,726
effective search space: 4514204488
effective search space used: 4514204488
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)