ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2332 good S KOG2722 Function unknown Predicted membrane protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2332 822820 821609 -404
(404 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YBR287w [S] KOG2722 Predicted membrane protein 384 e-106
SPAC5D6.04 [S] KOG2722 Predicted membrane protein 243 4e-64
At1g76520 [S] KOG2722 Predicted membrane protein 73 7e-13
At5g01990 [S] KOG2722 Predicted membrane protein 54 4e-07
At1g71090 [S] KOG2722 Predicted membrane protein 51 3e-06
>YBR287w [S] KOG2722 Predicted membrane protein
Length = 427
Score = 384 bits (987), Expect = e-106
Identities = 200/420 (47%), Positives = 283/420 (66%), Gaps = 26/420 (6%)
Query: 10 SFLQLSWTTFQSVLIVFIIAVSGFLSAYAGILPKEGQKIVSMLNVSLLTPCLIFSKLARS 69
SF L++ F+SVL V IIA++GF SA +G+LPK+ QKI+S+LNV L TPCLIFSKLA+S
Sbjct: 6 SFAHLAYLVFESVLQVVIIALAGFWSASSGLLPKQSQKIISLLNVDLFTPCLIFSKLAKS 65
Query: 70 LSFGTLIQLYVVPIFYTALVSTSYFSASLVSKILKLDGDETNFVIGTSVFPNSNSLPVSL 129
LS + ++ ++PIF+ S+ S ++S+IL LD DETNFV+ SVF NSNSLPVSL
Sbjct: 66 LSMAKIFEIAIIPIFFGLTTGISFISGKIMSRILDLDKDETNFVVANSVFGNSNSLPVSL 125
Query: 130 MMSLAYSLPQLKWPELPNDSGDNIASRGVLYLIIFQQIDQTLRWSWGLNKLLRWS----- 184
+SLAY+LP L W ++PND+ DN+ASRG+LYL+IFQQI Q LRWSWG NKL++WS
Sbjct: 126 TLSLAYTLPNLTWDQIPNDNRDNVASRGILYLLIFQQIGQMLRWSWGYNKLMKWSGENTQ 185
Query: 185 ----TEIELSIEDTMEQNADRLLTRGSE-----DEANN--------LTKVGSKLRYH--- 224
++++ +E T + + L+ E +E NN + +G K+
Sbjct: 186 HMPPSQVQSLLERTPNIDNEELVNEEQEEQELLEEENNRMNSSFLSSSSIGDKIWQKSCT 245
Query: 225 -WNNLLSCMNGPLYSIMFSILVASIRPLQEQLFESNGFLKNTLTSAIDQMADVSIPLILV 283
+ + + +N PLYS++F+++VA+I PLQ +LF +GF+ NT A+ Q+ VSIPLILV
Sbjct: 246 VFERIRANLNPPLYSMIFAVVVAAIGPLQRELFMEDGFINNTFAEAVTQLGSVSIPLILV 305
Query: 284 VLGANLCPSSTTPLGTHNRKRIVLASIISRMXXXXXXXXXXXXFTVKKLRKSILTDPVFI 343
VLG+NL PS+ T + ++++ SII RM VK + SIL DP+F+
Sbjct: 306 VLGSNLYPSAEVFPKTVHHSKLLIGSIIGRMILPSCFLLPIIAIAVKYINVSILDDPIFL 365
Query: 344 LVSFLLTASPPAIQLTQLTQLNEFFEFEIVNVLFWTYVVMTLPVTILMVTIALRVMDWAS 403
+V FLLT SPPAIQLTQ+TQLNEFFE E+ ++LFW Y V++LPV+I++V+ A+ V+ WA+
Sbjct: 366 VVGFLLTVSPPAIQLTQITQLNEFFEAEMADILFWGYAVLSLPVSIIVVSGAIYVLQWAN 425
>SPAC5D6.04 [S] KOG2722 Predicted membrane protein
Length = 452
Score = 243 bits (620), Expect = 4e-64
Identities = 145/430 (33%), Positives = 225/430 (51%), Gaps = 48/430 (11%)
Query: 20 QSVLIVFIIAVSGFLSAYAGILPKEGQKIVSMLNVSLLTPCLIFSKLARSLSFGTLIQLY 79
+S L V +IA+ G++ A G LP++ QK++S LNV TPCL+F K+ L+ LI L
Sbjct: 21 ESDLEVIVIALGGYVLAKKGFLPRDAQKVISSLNVYFFTPCLVFEKVGNGLNLKMLIDLS 80
Query: 80 VVPIFYTALVSTSYFSASLVSKILKLDGDETNFVIGTSVFPNSNSLPVSLMMSLAYSLPQ 139
++P+FY + + S + L++K+ +L + NF F NSNSLP++L+ SLA ++
Sbjct: 81 LLPVFYVIISAASILISFLLAKLFRLTPRQRNFATACITFQNSNSLPLALVSSLATTVKD 140
Query: 140 LKWPELPNDSGDNIASRGVLYLIIFQQIDQTLRWSWGLNKLL--------------RWS- 184
L W ++P+D+ D +ASRG++YL+IF Q+ Q LRWS+G LL WS
Sbjct: 141 LLWDKIPDDTPDKVASRGIMYLLIFSQLGQALRWSYGYRILLSPNQPEDPLPIGNRSWSH 200
Query: 185 ---TEIEL-----------SIEDTMEQNA--------------DRLLTRGSEDEANNLTK 216
E E+ ++++++ N D + S +E
Sbjct: 201 SDVNEEEIQNLLASSANVDGVQNSVQANEGSTVQTDSSAISKNDNVQVETSNEEVGGFGA 260
Query: 217 VGSKLRYHWNNLLSCMNGPLYSIMFSILVASIRPLQEQLFESNGFLKNTLTSAIDQMADV 276
SK+ LL + PLYS+ ++ +A + PLQ FE F++ ++TS I V
Sbjct: 261 ASSKISKFIVLLLDFFSPPLYSLFIALFIAVVPPLQRFFFEEGSFVEGSITSGIRMAGQV 320
Query: 277 SIPLILVVLGANLCP--SSTTP---LGTHNRKRIVLASIISRMXXXXXXXXXXXXFTVKK 331
++P+ILVVLGA+L S T P + +N R+++ ++ RM
Sbjct: 321 AVPMILVVLGASLATDISKTEPTQEVRKNNDTRVIIVCLLGRMVVVPLALLPAFSLLSYF 380
Query: 332 LRKSILTDPVFILVSFLLTASPPAIQLTQLTQLNEFFEFEIVNVLFWTYVVMTLPVTILM 391
S + DPVF++V FLL SP AIQLTQ+ QLN FE E VL+W+Y V T P ++L+
Sbjct: 381 SEISTVDDPVFVVVIFLLVGSPTAIQLTQICQLNGVFERECAKVLWWSYAVFTPPNSLLL 440
Query: 392 VTIALRVMDW 401
+L V+ W
Sbjct: 441 AFASLLVVKW 450
>At1g76520 [S] KOG2722 Predicted membrane protein
Length = 390
Score = 73.2 bits (178), Expect = 7e-13
Identities = 77/394 (19%), Positives = 170/394 (42%), Gaps = 28/394 (7%)
Query: 9 ISFLQLSWTTFQSVLIVFIIAVSGFLSAYAGI--LPKEGQKIVSMLNVSLLTPCLIFSKL 66
+ L+L T+ + V+ + +I GF A G+ L + +K ++ + + +P LI S+L
Sbjct: 2 VKLLELFITSSKPVVEILLITSVGFYMALDGVNLLGHDARKYLNNIVFYVFSPSLIGSRL 61
Query: 67 ARSLSFGTLIQLYVVPIFYTALVSTSYFSASLVSKILKLDGDETNFVIGTSVFPNSNSLP 126
A S+++ +L++++ +P+ +V I K ++G N ++P
Sbjct: 62 ADSVTYESLVKMWFMPVNVLLTFIIGSLLGWIVIVITKPPSHLRGLILGCCAAGNLGNMP 121
Query: 127 VSLMMSLAYSLPQLKWPELPNDSGDNIASRGVLYLIIFQQIDQTLRWSWGLNKLLRWSTE 186
+ ++ ++ K P ++ G+ Y+ + + W++ N L+R +
Sbjct: 122 LIIIPAVC------KEKGGPFGDPESCQKYGMGYVALSMAMGSIYIWTYVYN-LMRVLSN 174
Query: 187 IELSIEDTMEQNADRL---LTRGSEDEANNLTKVGSKLRYHWNNLLSCMN------GPLY 237
+ ++E N D L E+E N K++ +L +N
Sbjct: 175 SPVETPPSVESNYDSYKVPLISSKEEENNQKAGRWEKVKRRLVSLSQKVNLKTIFAPSTI 234
Query: 238 SIMFSILVASIRPLQEQLFESNGFLKNTLTSAIDQMADVSIPLILVVLGANLCPSSTTPL 297
+ M ++++ I PL++ + + L+ L ++ + D ++P + +++G NL +
Sbjct: 235 AAMIALVIGLITPLRKLIIGTEAPLR-VLQDSVTLVGDGAVPAMTMIIGGNLLKGLRS-- 291
Query: 298 GTHNRKRIVLASIISRMXXXXXXXXXXXXFTVKKLRKS--ILTDPVFILVSFLLTASPPA 355
+ ++SII + V+ K + ++P++ V L A PPA
Sbjct: 292 -----SGMKMSSIIGVLVARYVLLPMSGVLIVRGAYKLDLVTSEPLYQFVLLLQYAVPPA 346
Query: 356 IQLTQLTQLNEFFEFEIVNVLFWTYVVMTLPVTI 389
+ L +TQL E E ++ WTY + ++ +T+
Sbjct: 347 MNLGTITQLFGTGESECSVIMLWTYSLASIALTV 380
>At5g01990 [S] KOG2722 Predicted membrane protein
Length = 431
Score = 53.9 bits (128), Expect = 4e-07
Identities = 65/319 (20%), Positives = 128/319 (39%), Gaps = 39/319 (12%)
Query: 25 VFIIAVSGFLSA--YAGILPKEGQKIVSMLNVSLLTPCLIFSKLARSLSFGTLIQLYVVP 82
VF + G L A Y ILP G+K+++ L SLL PCLIFS+L ++++ ++Q + +P
Sbjct: 38 VFTMCFLGLLMASKYVNILPPSGRKLLNGLVFSLLLPCLIFSQLGQAVTLQKMLQWWFIP 97
Query: 83 IFYTALVSTSYFSASLVSKILKLDGDETNFVIGTSVFPNSNSLPVSLMMSLAYSLPQLKW 142
+ + +V+ I++ F I N ++P+ L+ +L
Sbjct: 98 VNVVLGTISGSIIGFIVASIVRPPYPYFKFTIIQIGVGNIGNVPLVLLAALCRDTSN--- 154
Query: 143 PELPNDSGDNIASRGVLYLIIFQQIDQTLRWSWGLNKLL----RWSTEIE------LSIE 192
P + + G Y+ Q + + +++ + E E L ++
Sbjct: 155 ---PFGDSEKCSIDGTAYISFGQWVGAIILYTYVYQMFAPPPEGFDAEEENLALKTLPVD 211
Query: 193 DTMEQ--------------NADRLLTRGSEDEANNLTKVGSKLR-----YHWNNLLSCMN 233
EQ D L + +E +++ G + Y L +
Sbjct: 212 AAPEQVPLLTQNFPKDFSPTQDLLPVQSTEPRGRGVSRKGKIAQIFVFLYEKLKLKQIVQ 271
Query: 234 GPLYSIMFSILVASIRPLQEQLFESNGFLKNTLTSAIDQMADVSIPLILVVLGANLCPS- 292
+ + + ++++ +I P ++L +NG T + + D IP IL+ LG NL
Sbjct: 272 PAIVASILAMILGAI-PFTKKLIFTNGAPLFFFTDSCMILGDAMIPCILLALGGNLINGP 330
Query: 293 STTPLGTHNRKRIVLASII 311
++ LG I++ ++
Sbjct: 331 GSSKLGFKTTAAIIIGRLV 349
>At1g71090 [S] KOG2722 Predicted membrane protein
Length = 457
Score = 51.2 bits (121), Expect = 3e-06
Identities = 36/148 (24%), Positives = 70/148 (46%), Gaps = 10/148 (6%)
Query: 22 VLIVFIIAVSGFLSAY--AGILPKEGQKIVSMLNVSLLTPCLIFSKLARSLSFGTLIQLY 79
+L + + V G L A+ ++P+ +++S L +L PCLIF++L S++ ++Q +
Sbjct: 23 LLKLICLTVIGLLLAHPKTQLVPRATFRLLSKLVFALFLPCLIFTELGESITLDNIVQWW 82
Query: 80 VVPIFYTALVSTSYFSASLVSKILKLDGDETNFVIGTSVFPNSNSLPVSLMMSLAYSLPQ 139
+P+ LV I + + F I + F N+ +L ++++ S+ ++
Sbjct: 83 FIPVNVLLSAVVGSLIGYLVVLICRPPPEFNRFTIVMTAFGNTGNLLLAIVSSVCHT--- 139
Query: 140 LKWPELPNDSGDNIASRGVLYLIIFQQI 167
N G N SRGV Y+ Q +
Sbjct: 140 -----KTNPFGPNCNSRGVSYVSFAQWV 162
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.323 0.136 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,610,176
Number of Sequences: 60738
Number of extensions: 796567
Number of successful extensions: 1956
Number of sequences better than 1.0e-05: 5
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1948
Number of HSP's gapped (non-prelim): 9
length of query: 404
length of database: 30,389,216
effective HSP length: 109
effective length of query: 295
effective length of database: 23,768,774
effective search space: 7011788330
effective search space used: 7011788330
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)