ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2360 good H KOG1380 Coenzyme transport and metabolism Heme A farnesyltransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2360 829526 830881 452
(452 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YPL172c [H] KOG1380 Heme A farnesyltransferase 578 e-165
SPBC365.02c [H] KOG1380 Heme A farnesyltransferase 264 2e-70
7297663 [H] KOG1380 Heme A farnesyltransferase 228 1e-59
Hs17921982 [H] KOG1380 Heme A farnesyltransferase 218 1e-56
CE24292 [H] KOG1380 Heme A farnesyltransferase 186 6e-47
At2g44520 [H] KOG1380 Heme A farnesyltransferase 161 2e-39
>YPL172c [H] KOG1380 Heme A farnesyltransferase
Length = 462
Score = 578 bits (1491), Expect = e-165
Identities = 282/406 (69%), Positives = 327/406 (80%), Gaps = 5/406 (1%)
Query: 43 NNNSEQNQVHINTAPIEFTANLLAGKNETEAETEGLKGTALKALRCND---TTSDSEPQH 99
+ N Q HINTAPIEFT N G + + ++ TA+K LRC D T+S SE
Sbjct: 47 SRNHGGKQQHINTAPIEFTPNFGYGDRTSNCNKK-VESTAMKTLRCTDDISTSSGSEATT 105
Query: 100 NMEN-LPFKVKLVDKGQRKQIKESKARVEPRPEVSLKKTYIEPYLQLSKPRLTVLVMLSA 158
+ LPF VKLVD RK + S A E +LKK I PYLQL+KPRLT+LVMLSA
Sbjct: 106 DASTQLPFNVKLVDPMVRKSKRPSHAISEGLNMKTLKKKVIMPYLQLTKPRLTILVMLSA 165
Query: 159 ICSYALSPYPATVLQLLSLTVGTTLCSASANAINMGREPDFDRQMIRTQARPVVRGIVSP 218
ICSYALSPYPA+V +LL LTVGTTLCS SANAINMGREP+FDRQM+RTQARPVVRG V+P
Sbjct: 166 ICSYALSPYPASVNELLCLTVGTTLCSGSANAINMGREPEFDRQMVRTQARPVVRGDVTP 225
Query: 219 NQAFKFATAAGTLGTSILWLGVNPTVAFLGFSNIALYAWLYTSLKRKHIINTWVGALVGA 278
QAF+FA GTLG SIL+ GVNPTVA LG SNIALY W YTS+KRKHIINTW+GALVG
Sbjct: 226 TQAFEFAALIGTLGVSILYFGVNPTVAILGASNIALYGWAYTSMKRKHIINTWLGALVGM 285
Query: 279 IPPLMGWAASSPLSHPGAWCLAGLLYAWQFPHFNTLSHNIRNEYKNAGYVMTAWKNPKLN 338
+PPLMGWAA+SPLSHPG+WCLAGLL+AWQFPHFNTLSHNIRNEYKNAGYVMTAWKNP LN
Sbjct: 286 VPPLMGWAAASPLSHPGSWCLAGLLFAWQFPHFNTLSHNIRNEYKNAGYVMTAWKNPLLN 345
Query: 339 ARVALRYSLLMFPLCFGLSYFNVTDWYYQLDSAFVNAWMSLWAFKFYFQQKRNYSKEIYN 398
ARV+LRYS+LMFPLCFGLSYFN+TDWYYQ+DS +NAW++ WAFKFY+QQ+ NYS +
Sbjct: 346 ARVSLRYSILMFPLCFGLSYFNITDWYYQIDSGLINAWLTFWAFKFYWQQRINYSAKTLK 405
Query: 399 NKTEFNKGLAMANVYARRTFWVSVLHLPAVLILAILHKKDRWDWLF 444
+ +FNKGL++AN+YAR+TF SVLHLPA+LILAI+HKK RWDW++
Sbjct: 406 DNVKFNKGLSVANIYARKTFMASVLHLPAILILAIIHKKGRWDWIY 451
>SPBC365.02c [H] KOG1380 Heme A farnesyltransferase
Length = 387
Score = 264 bits (675), Expect = 2e-70
Identities = 144/296 (48%), Positives = 183/296 (61%), Gaps = 26/296 (8%)
Query: 142 YLQLSKPRLTVLVMLSAICSYALSPYPATVLQLLS-LTVGTTLCSASANAINMGREPDFD 200
+L+L KPRLTVLV+LS + SYAL+PYP L+ LT+GT LCS SANA N EP D
Sbjct: 87 FLELGKPRLTVLVVLSTMSSYALAPYPGLSFNTLAWLTMGTALCSISANAFNQSMEPMLD 146
Query: 201 RQMIRTQARPVVRGIVSPNQAFKFATAAGTLGTSILWLGVNPTVAFLGFSNIALYAWLYT 260
QM RT++RP+ RG + P A+ FAT G GTS+ +L VNPTV +LG NI LY +YT
Sbjct: 147 CQMARTRSRPIPRGAIRPEYAWLFATLTGIAGTSMSFL-VNPTVGWLGLGNIVLYMGIYT 205
Query: 261 SLKRKHIINTWVGALVGAIPPLMGWAASSP---LSHPGAWCLAGLLYAWQFPHFNTLSHN 317
LKR I+NTWVG+LVGAIPPLMGWAA S LSHPG A +L+AWQFPHFN S
Sbjct: 206 PLKRISIVNTWVGSLVGAIPPLMGWAACSGGDLLSHPGGLITAAMLFAWQFPHFNAFSTM 265
Query: 318 IRNEYKNAGYVMTAWKNPKLNARVALRYSLLMFPLCFGLSYFNVTDWYYQLDSAFVNAWM 377
++++YK GY M AWKNP LNARV+LRY+L PL + + +Y + + N ++
Sbjct: 266 VKDDYKKCGYQMMAWKNPALNARVSLRYALAFLPLSYAYISTGLVGPWYAVPATGTNMFL 325
Query: 378 SLWAFKFYFQQKRNYSKEIYNNKTEFNKGLAMANVYARRTFWVSVLHLPAVLILAI 433
A+KFY + RNY AR F+ S+LHLP + L +
Sbjct: 326 IARAWKFY--RNRNYQN-------------------ARSLFFASLLHLPLLFTLTL 360
>7297663 [H] KOG1380 Heme A farnesyltransferase
Length = 391
Score = 228 bits (581), Expect = 1e-59
Identities = 136/342 (39%), Positives = 194/342 (55%), Gaps = 31/342 (9%)
Query: 114 GQRKQIKESKARVEPRPEVSLKKTYIEPYLQLSKPRLTVLVMLSAICSYALSPYPATVLQ 173
G+ + + +S+ P P KT + Y +LSK RLT LV+++ + YA++P
Sbjct: 49 GKVQHLPDSQITWMPSPYTMPGKT-LSQYKKLSKFRLTSLVVITTMGGYAMAPAAFDPTT 107
Query: 174 LLSLTVGTTLCSASANAINMGREPDFDRQMIRTQARPVVRGIVSPNQAFKFATAAGTLGT 233
T+GT L SA+ANAIN E FD QM RT+ R +V G ++P A FA + T G
Sbjct: 108 FAMCTLGTGLVSAAANAINQYHEVPFDSQMSRTKNRVLVTGQMTPLHAVTFAAVSATAGL 167
Query: 234 SILWLGVNPTVAFLGFSNIALYAWLYTSLKRKHIINTWVGALVGAIPPLMGWAASSPLSH 293
S+L+ GVN A LG N+ LY +YT +KR I+NTWVG++VGAIPPLMGWA +
Sbjct: 168 SMLYFGVNGLTAALGAGNLFLYTTIYTPMKRISIVNTWVGSIVGAIPPLMGWAGCAATLD 227
Query: 294 PGAWCLAGLLYAWQFPHFNTLSHNIRNEYKNAGYVMTAWKNPKLNARVALRYSLLMFPLC 353
GA LAG+LYAWQFPHFN LS N+R +Y AGY M A NP L R ALRYS+ + L
Sbjct: 228 AGAMILAGVLYAWQFPHFNALSWNLRPDYSRAGYRMMAVTNPGLCRRTALRYSVAIVGLS 287
Query: 354 FGLSYFNVTDWYYQLDSAFVNAWMSLWAFKFYFQQKRNYSKEIYNNKTEFNKGLAMANVY 413
+VT++++ L++ +NA+ + A+KF+ + S
Sbjct: 288 AMAPVLDVTNYWFALETLPLNAYFAYLAYKFHEKSDSGSS-------------------- 327
Query: 414 ARRTFWVSVLHLPAVLILAILHKKDRWDWLF------EDKKQ 449
R+ F S++HLPA+++L + +KK +W F E+KK+
Sbjct: 328 -RKLFRFSLIHLPALMLLFLTNKK---EWYFSKPAGEENKKE 365
>Hs17921982 [H] KOG1380 Heme A farnesyltransferase
Length = 443
Score = 218 bits (555), Expect = 1e-56
Identities = 133/350 (38%), Positives = 186/350 (53%), Gaps = 28/350 (8%)
Query: 95 SEPQHNMENLPFKVKLVDKGQRKQIKES-KARVEPRPEVSLKKTYIEPY------LQLSK 147
S P ++ P + +L++ I++S E + E K+ ++ Y +LSK
Sbjct: 103 SPPSLSLSRKPNEKELIELEPDSVIEDSIDVGKETKEEKRWKEMKLQVYDLPGILARLSK 162
Query: 148 PRLTVLVMLSAICSYALSPYPATVLQLLSLTVGTTLCSASANAINMGREPDFDRQMIRTQ 207
+LT LV+ + +AL+P P L +VGT L S +AN+IN E FD M RT+
Sbjct: 163 IKLTALVVSTTAAGFALAPGPFDWPCFLLTSVGTGLASCAANSINQFFEVPFDSNMNRTK 222
Query: 208 ARPVVRGIVSPNQAFKFATAAGTLGTSILWLGVNPTVAFLGFSNIALYAWLYTSLKRKHI 267
RP+VRG +SP A FAT G +IL LGVNP LG NI LY YT LKR I
Sbjct: 223 NRPLVRGQISPLLAVSFATCCAVPGVAILTLGVNPLTGALGLFNIFLYTCCYTPLKRISI 282
Query: 268 INTWVGALVGAIPPLMGWAASSPLSHPGAWCLAGLLYAWQFPHFNTLSHNIRNEYKNAGY 327
NTWVGA+VGAIPP+MGW A++ GA+ L G+LY+WQFPHFN LS +R +Y GY
Sbjct: 283 ANTWVGAVVGAIPPVMGWTAATGSLDAGAFLLGGILYSWQFPHFNALSWGLREDYSRGGY 342
Query: 328 VMTAWKNPKLNARVALRYSLLMFPLCFGLSYFNVTDWYYQLDSAFVNAWMSLWAFKFYFQ 387
M + +P L RVALR+ L + L ++T W + + + +NA++S F+FY
Sbjct: 343 CMMSVTHPGLCRRVALRHCLALLVLSAAAPVLDITTWTFPIMALPINAYISYLGFRFYVD 402
Query: 388 QKRNYSKEIYNNKTEFNKGLAMANVYARRTFWVSVLHLPAVLILAILHKK 437
R S RR F+ S+ HLP +L+L + K+
Sbjct: 403 ADRRSS---------------------RRLFFCSLWHLPLLLLLMLTCKR 431
>CE24292 [H] KOG1380 Heme A farnesyltransferase
Length = 297
Score = 186 bits (472), Expect = 6e-47
Identities = 109/269 (40%), Positives = 151/269 (55%), Gaps = 22/269 (8%)
Query: 164 LSPYPATVLQLLSLTVGTTLCSASANAINMGREPDFDRQMIRTQARPVVRGIVSPNQAFK 223
++P P L++ TVGT L S++ANA N E +D QM RTQ R +V SP AF
Sbjct: 1 MAPVPVAADSLIAATVGTFLLSSAANACNQLLEAPYDAQMRRTQTRVLVVHRFSPLHAFT 60
Query: 224 FATAAGTLGTSILWLGVNPTVAFLGFSNIALYAWLYTSLKRKHIINTWVGALVGAIPPLM 283
FA G G +L NP A LG N +YA +YT +KR HI TW GA+VGAIPPLM
Sbjct: 61 FAGVTGLTGIGLLAYYANPLAAALGALNWFIYAGVYTPMKRSHIGCTWAGAVVGAIPPLM 120
Query: 284 GWAASSPLSHPGAWCLAGLLYAWQFPHFNTLSHNIRNEYKNAGYVMTAWKNPKLNARVAL 343
G+AA++ P AWCLA +L++WQFPHFN LS N+R +Y AGY + N +L +L
Sbjct: 121 GYAAATGYLDPAAWCLATILFSWQFPHFNGLSWNLRGDYSKAGYRVMCVTNERLCRVTSL 180
Query: 344 RYSLLMFPLC-FGLSYFNVTDWYYQLDSAFVNAWMSLWAFKFYFQQKRNYSKEIYNNKTE 402
R+S+ + LC ++T + +DS VNA++ ++KFY K +K
Sbjct: 181 RHSVALLGLCSIAAPLTDLTTLTFAIDSLPVNAYLVYLSYKFY---KAPDAKN------- 230
Query: 403 FNKGLAMANVYARRTFWVSVLHLPAVLIL 431
+R+ F+ S+LHLP V++L
Sbjct: 231 -----------SRKLFFYSLLHLPLVMLL 248
>At2g44520 [H] KOG1380 Heme A farnesyltransferase
Length = 434
Score = 161 bits (408), Expect = 2e-39
Identities = 112/307 (36%), Positives = 158/307 (50%), Gaps = 30/307 (9%)
Query: 138 YIEPYLQLSKPRLTVLVMLSAICSYALSPYPATVL--QLLSLTVGTTLCSASANAINMGR 195
Y Y +LSK +L++LV+ ++ Y L A + L GT + +ASAN++N
Sbjct: 94 YARCYWELSKAKLSMLVVATSGTGYILGTGNAAISFPGLCYTCAGTMMIAASANSLNQIF 153
Query: 196 EPDFDRQMIRTQARPVVRGIVSPNQAFKFATAAGTLGTSILWLGVNPTVAFLGFSNIALY 255
E D +M RT RP+ G +S A +AT AG G +L N A L +N+ LY
Sbjct: 154 EISNDSKMKRTMLRPLPSGRISVPHAVAWATIAGASGACLLASKTNMLAAGLASANLVLY 213
Query: 256 AWLYTSLKRKHIINTWVGALVGAIPPLMGWAASSPLSHPGAWCLAGLLYAWQFPHFNTLS 315
A++YT LK+ H INTWVGA+VGAIPPL+GWAA+S + L LY WQ PHF L+
Sbjct: 214 AFVYTPLKQLHPINTWVGAVVGAIPPLLGWAAASGQISYNSMILPAALYFWQIPHFMALA 273
Query: 316 HNIRNEYKNAGYVMTAWKNP--KLNARVALRYSLLMFPLCFGLSY----FNVTDWYYQLD 369
H RN+Y GY M + +P K A VALR M PL F ++Y + +T ++ L+
Sbjct: 274 HLCRNDYAAGGYKMLSLFDPSGKRIAAVALRNCFYMIPLGF-IAYDCESWGLTSSWFCLE 332
Query: 370 SAFVNAWMSLWAFKFYFQQKRNYSKEIYNNKTEFNKGLAMANVYARRTFWVSVLHLPAVL 429
S + ++ AF FY + R K AR+ F S+L LP +
Sbjct: 333 STLLTLAIAATAFSFY--RDRTMHK-------------------ARKMFHASLLFLPVFM 371
Query: 430 ILAILHK 436
+LH+
Sbjct: 372 SGLLLHR 378
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.321 0.134 0.414
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 26,752,825
Number of Sequences: 60738
Number of extensions: 1109677
Number of successful extensions: 2643
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 2626
Number of HSP's gapped (non-prelim): 8
length of query: 452
length of database: 30,389,216
effective HSP length: 110
effective length of query: 342
effective length of database: 23,708,036
effective search space: 8108148312
effective search space used: 8108148312
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)