ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2376 good R KOG0562 General function prediction only Predicted hydrolase (HIT family)
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII2376 835433 834465 -323 
         (323 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YOR258w [R] KOG0562 Predicted hydrolase (HIT family)                  155   6e-38
SPCC18.09c [R] KOG0562 Predicted hydrolase (HIT family)                93   5e-19
At5g01310_3 [R] KOG0562 Predicted hydrolase (HIT family)               53   7e-07

>YOR258w [R] KOG0562 Predicted hydrolase (HIT family)
          Length = 217

 Score =  155 bits (393), Expect = 6e-38
 Identities = 88/216 (40%), Positives = 123/216 (56%), Gaps = 15/216 (6%)

Query: 103 SFSIGLRPYVLCPERLPE--VLYFDDIATIIHDKFPKSEEHFLVLPRSHKISNSHPT-TI 159
           S+   L+ YV  PE + +  V YFDD  +II D FPKSE H L+LPR+ ++S SHPT  I
Sbjct: 2   SWRYALKNYVTSPETVNDDTVTYFDDKVSIIRDSFPKSECHLLILPRTMQLSRSHPTKVI 61

Query: 160 DNGIKVQLQWHIDWAKRFCWTQFIKKYDIK----------DISLKEKEAFLANFVQSGVH 209
           D   K + + +++ A    +  F +K+ IK          D  LK+K  F+ NFVQ G+H
Sbjct: 62  DAKFKNEFESYVNSAIDHIFRHFQEKFRIKKSDDDKDPCWDDILKDKNKFVRNFVQVGIH 121

Query: 210 STPSMANTHIHVMTRDXXXXXXXXXXXXXXXXSPFFIPWDELPV--LDLPGDKELSERYI 267
           S PSMAN HIHV+++D                + FFI WD+LP+   +L  DKE+   Y+
Sbjct: 122 SVPSMANLHIHVISKDFHSVRLKNKKHYNSFNTGFFISWDDLPLNGKNLGTDKEIETTYL 181

Query: 268 KNFEMICPYCSTNFKNRFAKLKEHLADEFNMRFVSK 303
           K  +++C YC  NF N+F+ LK+HL  EFN  F  K
Sbjct: 182 KEHDLLCCYCQRNFSNKFSLLKKHLELEFNSHFELK 217


>SPCC18.09c [R] KOG0562 Predicted hydrolase (HIT family)
          Length = 232

 Score = 93.2 bits (230), Expect = 5e-19
 Identities = 57/201 (28%), Positives = 96/201 (47%), Gaps = 4/201 (1%)

Query: 97  EKNRENSFSIGLRPYVLCPERLPEVLYFDDIATIIHDKFPKSEEHFLVLPRSHKISNSHP 156
           +K R+ SF   L+ Y+  PE    V+Y+DD   ++ D FPKS+ H L++ R   +++ HP
Sbjct: 27  KKYRKQSFRDNLKVYIESPESYKNVIYYDDDVVLVRDMFPKSKMHLLLMTRDPHLTHVHP 86

Query: 157 TTIDNGIKVQLQWHIDWAKRFCWTQFIKKYDIKDISLKEKEAFLANFVQSGVHSTPSMAN 216
             I    +  ++  + + +    +  I       +S +     L N+++ G H+ PSM N
Sbjct: 87  LEIMMKHRSLVEKLVSYVQGDL-SGLIFDEARNCLSQQLTNEALCNYIKVGFHAGPSMNN 145

Query: 217 THIHVMTRDXXXXXXXXXXXXXXXXSPFFIPWDELPVLDLPGDKELSERYIKNFEMICPY 276
            H+H+MT D                SPFF+  D  P  +LP    L+  + ++ +  C  
Sbjct: 146 LHLHIMTLDHVSPSLKNSAHYISFTSPFFVKID-TPTSNLPTRGTLTSLFQEDLK--CWR 202

Query: 277 CSTNFKNRFAKLKEHLADEFN 297
           C   F   F KLK HL +E++
Sbjct: 203 CGETFGRHFTKLKAHLQEEYD 223


>At5g01310_3 [R] KOG0562 Predicted hydrolase (HIT family)
          Length = 198

 Score = 52.8 bits (125), Expect = 7e-07
 Identities = 45/193 (23%), Positives = 83/193 (42%), Gaps = 21/193 (10%)

Query: 103 SFSIGLRPYVLCPERLPEVL--YFDDIATIIHDKFPKSEEHFLVLPRSHKISNSHPTTID 160
           ++++ L    + PER   V+  Y D+I  +I+D++PK+ +H LVL R   +        +
Sbjct: 1   TWALALHSIAMHPERHENVVLEYLDNIV-VINDQYPKARKHVLVLARQESLDGLEDVRKE 59

Query: 161 NGIKVQLQWHIDWAKRFCWTQFIKKYDIKDISLKEKEAFLANFVQSGVHSTPSMANTHIH 220
           N   +Q   ++         +++ ++  +D SL           + G HS PSM   H+H
Sbjct: 60  NLQLLQEMHNVG-------LKWVDRFQNEDASL---------IFRLGYHSVPSMRQLHLH 103

Query: 221 VMTRDXXXXXXXXXXXXXXXXSPFF-IPWDELPVLDLPGDKELSERYIKNFEMICPYCST 279
           V+++D                + FF    D L  ++  G   ++   +   E+ C  C +
Sbjct: 104 VISQDFNSDSLKNKKHWNSFTTSFFRDSVDVLEEVNSQGKANVASEDLLKGELRCNRCRS 163

Query: 280 NFKNRFAKLKEHL 292
              N   KLK H+
Sbjct: 164 AHPN-IPKLKSHV 175


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.322    0.138    0.438 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,814,195
Number of Sequences: 60738
Number of extensions: 916036
Number of successful extensions: 2045
Number of sequences better than 1.0e-05: 3
Number of HSP's better than  0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2038
Number of HSP's gapped (non-prelim): 4
length of query: 323
length of database: 30,389,216
effective HSP length: 106
effective length of query: 217
effective length of database: 23,950,988
effective search space: 5197364396
effective search space used: 5197364396
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)