ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2457 good V KOG1529 Defense mechanisms Mercaptopyruvate sulfurtransferase/thiosulfate sulfurtransferase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2457 860413 861309 299
(299 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR251c [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfa... 317 1e-86
At1g79230 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosul... 169 3e-42
At1g16460 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosul... 162 5e-40
Hs13489091 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosu... 145 5e-35
Hs17402865 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosu... 130 3e-30
SPCP25A2.01c [V] KOG1529 Mercaptopyruvate sulfurtransferase/thio... 127 3e-29
SPCC4B3.01 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosu... 87 2e-17
CE19917 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfa... 70 3e-12
CE19920 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfa... 55 9e-08
>YOR251c [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 304
Score = 317 bits (813), Expect = 1e-86
Identities = 152/295 (51%), Positives = 204/295 (68%), Gaps = 2/295 (0%)
Query: 1 MTLYKLLTPKAFAELLSKETKRRIIPVDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDG 60
M L+ L++PKAF +L++ E RI+PVD+TWYLP+ K +FL + R+ +++FDID
Sbjct: 1 MPLFDLISPKAFVKLVASEKVHRIVPVDATWYLPSWKLDNKVDFLTKPRIPNSIFFDIDA 60
Query: 61 VKDMKSEYPHMLPDLSTFNKSMSELGLKKDDILVVYDRIGNFSAPRCAWTLAVLGHDPVY 120
+ D KS YPHM P F+ +MS LG++KDDILVVYDR+GNFS+PRCAWTL V+GH VY
Sbjct: 61 ISDKKSPYPHMFPTKKVFDDAMSNLGVQKDDILVVYDRVGNFSSPRCAWTLGVMGHPKVY 120
Query: 121 LLNNFPALKLSGYPVETSKVTSFTPYEMSEYVSDVDLAEKETVSFEDMQKLVSTGDI-KN 179
LLNNF + YP+++SKV +F+PY S Y S +KE V +E+M +LV +G++ K
Sbjct: 121 LLNNFNQYREFKYPLDSSKVAAFSPYPKSHYESSESFQDKEIVDYEEMFQLVKSGELAKK 180
Query: 180 YQVLDARSLQRFTGEAPEPRPGLSSGHIPGVQPLPFLEVLNSD-KTFTSDREEMLKKIQD 238
+ DARSL RF G PEPR + SGHIPG QPLP+ +L+ + KT+ E + ++
Sbjct: 181 FNAFDARSLGRFEGTEPEPRSDIPSGHIPGTQPLPYGSLLDPETKTYPEAGEAIHATLEK 240
Query: 239 FVSQTGNEFETDKPTIAMCGTGVTGVIIKTALEISGVKNVRLYDGSWTEWVMKDG 293
+ + KPTI CGTGV+GVIIKTALE++GV NVRLYDGSWTEWV+K G
Sbjct: 241 ALKDFHCTLDPSKPTICSCGTGVSGVIIKTALELAGVPNVRLYDGSWTEWVLKSG 295
>At1g79230 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 322
Score = 169 bits (429), Expect = 3e-42
Identities = 105/275 (38%), Positives = 145/275 (52%), Gaps = 21/275 (7%)
Query: 27 VDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDGVKDMKSEYPHMLPDLSTFNKSMSELG 86
+D++WY+P+ R+ QE+ + A++FD+DG+ D K+ PHMLP F S LG
Sbjct: 41 LDASWYMPDEQRNPIQEY-QVAHIPRALFFDLDGISDRKTSLPHMLPTEEAFAAGCSALG 99
Query: 87 LKKDDILVVYDRIGNFSAPRCAWTLAVLGHDPVYLLN-NFPALKLSGYPVETSK-----V 140
+ D +VVYD G FSA R W V GH+ V++L+ P + SGY VE+S +
Sbjct: 100 IDNKDEVVVYDGKGIFSAARVWWMFRVFGHEKVWVLDGGLPRWRASGYDVESSASGDAIL 159
Query: 141 TSFTPYEMSEYVSDVDLAEKETVSFEDMQKLVSTGD-IKN------YQVLDARSLQRFTG 193
+ E E + T + LV T D +KN YQ +DARS RF G
Sbjct: 160 KASAASEAIEKIYQGQTVSPITFQTKFQPHLVWTLDQVKNNMEDPTYQHIDARSKARFDG 219
Query: 194 EAPEPRPGLSSGHIPGVQPLPFLEVLNSDKTFTSDREEMLKKIQDFVSQTGNEFETDKPT 253
APEPR G+ SGHIPG + +PF ++ +S T EE+ K+ + DKP
Sbjct: 220 TAPEPRKGIRSGHIPGSKCIPFPQMFDSCNTLL-PAEELKKRFDQ------EDISLDKPI 272
Query: 254 IAMCGTGVTGVIIKTALEISGVKNVRLYDGSWTEW 288
+A CGTGVT I+ L G +V +YDGSWTEW
Sbjct: 273 MASCGTGVTACILAMGLHRLGKTDVPIYDGSWTEW 307
>At1g16460 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 318
Score = 162 bits (410), Expect = 5e-40
Identities = 101/276 (36%), Positives = 151/276 (54%), Gaps = 23/276 (8%)
Query: 27 VDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDGVKDMKSEYPHMLPDLSTFNKSMSELG 86
+D++WY+ + R+ QE+ + GA++FD++G+ D K+ HMLP F S LG
Sbjct: 39 LDASWYMAHEQRNPIQEY-QVAHIPGALFFDLNGIADRKTNLRHMLPSEEAFAAGCSALG 97
Query: 87 LKKDDILVVYDRIGNFSAPRCAWTLAVLGHDPVYLLN-NFPALKLSGYPVETSKVTSFTP 145
++ +D +VVYD +G FSA R W V GHD V++L+ P + SGY VE+S V++
Sbjct: 98 IENNDGVVVYDGMGLFSAARVWWMFRVFGHDKVWVLDGGLPKWRASGYDVESS-VSNDAI 156
Query: 146 YEMSEYVSDVD-LAEKETVS---FED---------MQKLVSTGDIKNYQVLDARSLQRFT 192
+ S ++ + + +T+S F+ + ++ + K YQ +DARS RF
Sbjct: 157 LKASAATEAIEKIYQGQTISPITFQTKFRPHLVLALDQVKENIEDKTYQHIDARSKARFD 216
Query: 193 GEAPEPRPGLSSGHIPGVQPLPFLEVLNSDKTFTSDREEMLKKIQDFVSQTGNEFETDKP 252
G APEP GL SGHIPG + +PF + +S +T EE+ K+ + + D P
Sbjct: 217 GIAPEPWKGLPSGHIPGSKCVPFPLMFDSSQTLL-PAEELKKQFEQ------EDISLDSP 269
Query: 253 TIAMCGTGVTGVIIKTALEISGVKNVRLYDGSWTEW 288
A CGTGVT I+ L G NV +YDGSWTEW
Sbjct: 270 IAASCGTGVTACILALGLYRLGKTNVAIYDGSWTEW 305
>Hs13489091 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 297
Score = 145 bits (367), Expect = 5e-35
Identities = 91/268 (33%), Positives = 139/268 (50%), Gaps = 20/268 (7%)
Query: 27 VDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDGVKDMKSEYPHMLPDLSTFNKSMSELG 86
+D++WYLP L R ++EF +E + GA +FDID D S Y HMLP F + LG
Sbjct: 32 LDASWYLPKLGRDARREF-EERHIPGAAFFDIDQCSDRTSPYDHMLPGAEHFAEYAGRLG 90
Query: 87 LKKDDILVVYDRI--GNFSAPRCAWTLAVLGHDPVYLLNNFPALKL-SGYPVETSKVTSF 143
+ +V+YD G +SAPR W GH V LL+ L P+ + K
Sbjct: 91 VGAATHVVIYDASDQGLYSAPRVWWMFRAFGHHAVSLLDGGLRHWLRQNLPLSSGK---- 146
Query: 144 TPYEMSEYVSDVDLAEKETVSFEDMQKLVSTGDIKNYQVLDARSLQRFTGEAPEPRPGLS 203
+ +E+ + +D A +T +ED+++ + + + +QV+D+R+ RF G PEPR G+
Sbjct: 147 SQPAPAEFRAQLDPAFIKT--YEDIKENLES---RRFQVVDSRATGRFRGTEPEPRDGIE 201
Query: 204 SGHIPGVQPLPFLEVLNSDKTFTSDREEMLKKIQDFVSQTGNEFETDKPTIAMCGTGVTG 263
GHIPG +PF + L S + EE+ Q+ + + KP +A CG+GVT
Sbjct: 202 PGHIPGTVNIPFTDFL-SQEGLEKSPEEIRHLFQE------KKVDLSKPLVATCGSGVTA 254
Query: 264 VIIKTALEISGVKNVRLYDGSWTEWVMK 291
+ + G +V +YDGSW EW M+
Sbjct: 255 CHVALGAYLCGKPDVPIYDGSWVEWYMR 282
>Hs17402865 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 297
Score = 130 bits (326), Expect = 3e-30
Identities = 89/266 (33%), Positives = 132/266 (49%), Gaps = 22/266 (8%)
Query: 27 VDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDGVKDMKSEYPHMLPDLSTFNKSMSELG 86
+D++WY P R ++E+L E + GA +FDI+ +D S Y MLP + F + + LG
Sbjct: 32 LDASWYSPG-TREARKEYL-ERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLG 89
Query: 87 LKKDDILVVYD--RIGNFSAPRCAWTLAVLGHDPVYLLNN-FPALKLSGYPVETSKVTSF 143
+ +VVYD +G+F APR W V GH V +LN F G+PV +
Sbjct: 90 ISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTS------ 143
Query: 144 TPYEMSEYVSDVDLAEKETVSFEDMQKLVSTGDIKNYQVLDARSLQRFTGEAPEPRP-GL 202
E S V A + + ++++ + K +Q++D+RS RF G PEP GL
Sbjct: 144 ---EPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGL 200
Query: 203 SSGHIPGVQPLPFLEVLNSDKTFTSDREEMLKKIQDFVSQTGNEFETDKPTIAMCGTGVT 262
SGHI G +PF++ L D F EE+ Q + + +P IA C GVT
Sbjct: 201 DSGHIRGAVNMPFMDFLTEDG-FEKGPEELRALFQT------KKVDLSQPLIATCRKGVT 253
Query: 263 GVIIKTALEISGVKNVRLYDGSWTEW 288
+ A + G +V +YDGSW+EW
Sbjct: 254 ACHVALAAYLCGKPDVAVYDGSWSEW 279
>SPCP25A2.01c [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 195
Score = 127 bits (318), Expect = 3e-29
Identities = 69/179 (38%), Positives = 106/179 (58%), Gaps = 10/179 (5%)
Query: 18 KETKRRIIPVDSTWYLPNLNRSGKQEFLDEERLKGAVYFDIDGVKDMKSEYPHMLPDLST 77
K+ ++ + +D+TWYLP ++GK+E+L E RL GA YFDID KD K+ PHMLP
Sbjct: 22 KDNAQKTVLLDATWYLPTDTKNGKKEYL-ESRLPGAQYFDIDEAKDHKNPLPHMLPPADE 80
Query: 78 FNKSMSELGLKKDDILVVYDRIGNFSAPRCAWTLAVLGHDPVYLL-NNFPALKLSGYPVE 136
F + +LG+ ++ +++YDR G FS+PR WT V GH+ V+L N F A K G +E
Sbjct: 81 FASYVGKLGIDRNTNVIIYDRKGFFSSPRVFWTFKVFGHEHVFLFPNAFNAWKTEGLELE 140
Query: 137 TSKVTSFTP--YEMSEYVSDVDLAEKETVSFEDMQKLVSTGDIKNYQVLDARSLQRFTG 193
T + + P YE ++ D+ SF+D+ K++ + D ++DAR+ +RF G
Sbjct: 141 TGEPRTPKPVVYEGAKLNKDL------VASFDDIVKVIESPDAAGVHIVDARAHERFLG 193
>SPCC4B3.01 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 139
Score = 87.4 bits (215), Expect = 2e-17
Identities = 47/131 (35%), Positives = 72/131 (54%), Gaps = 5/131 (3%)
Query: 164 SFEDMQKLVSTGDIKNYQVLDARSLQRFTGEAPEPRPGLSSGHIPGVQPLPFLEVLNSDK 223
SF+D+ K++ + D ++DAR+ +RF G PE RPGL+SGHIP +PF E +
Sbjct: 5 SFDDIVKVIESPDAAGVHIVDARAHERFLGNVPESRPGLASGHIPTSINIPFTETTAAGI 64
Query: 224 TFTSDREEMLKKIQDFVSQTGNEFETDKPTIAMCGTGVTGVIIKTALEISGVKNVRLYDG 283
T E++ K V + + P I CG+GVT ++ AL+ G K+VR+YD
Sbjct: 65 TAPKPEEDLEK-----VFSSHGLTDKSVPIITSCGSGVTASVLFAALKECGFKDVRVYDE 119
Query: 284 SWTEWVMKDGE 294
SW+ + + E
Sbjct: 120 SWSGYGKRANE 130
>CE19917 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 324
Score = 70.5 bits (171), Expect = 3e-12
Identities = 72/250 (28%), Positives = 105/250 (41%), Gaps = 26/250 (10%)
Query: 47 EERLKGAVYFDIDGVKDMKSEY-PHMLPDLSTFNKSMSELGLKKDDILVVYDR---IGNF 102
+E + GAV+FD+D + SEY H L F K + LG+ D LV+Y G
Sbjct: 78 DEHIPGAVHFDLD-IGSYSSEYIKHDLYPPEHFQKYLRLLGVNNGDQLVIYSNGPASGMK 136
Query: 103 SAPRCAWTLAVLGHDPVYLLN-NFPALKLSGYPVETSKVTSFTPYEMSEYVSDVDLAEKE 161
A R WT + G V LLN K +G P + + VT + + + LA+ E
Sbjct: 137 FASRAYWTFKMYGFTTVSLLNGGMDVWKAAGGPTDNAVVTPKLGNVNVKSLDNTILAKFE 196
Query: 162 TVSFEDMQKLVSTGDIKNYQVLDARSLQRFTGEAP----EPRPGLSSGHIPGVQPLPFLE 217
+ F + + N LD R +F GE P P H+ G P +
Sbjct: 197 EIPFNNFAE-------TNTVYLDTRIRAQFDGEDPLATNFPGTKAKGSHVTGAINFPMAK 249
Query: 218 VLNSDKTFTSDREEMLKKIQDFVSQTGNEFETDKPTIAMCGTGVTGVIIKTALEISGVKN 277
V+ D F S ++ + + +S G + C TG+ +I ALE SG K
Sbjct: 250 VIGPD-GFISQQD-----VDEQISSLG--LTSASQVFVGCNTGMQASVIFVALERSGFK- 300
Query: 278 VRLYDGSWTE 287
+LY+GS E
Sbjct: 301 AKLYNGSMFE 310
>CE19920 [V] KOG1529 Mercaptopyruvate sulfurtransferase/thiosulfate
sulfurtransferase
Length = 221
Score = 55.5 bits (132), Expect = 9e-08
Identities = 49/184 (26%), Positives = 82/184 (43%), Gaps = 21/184 (11%)
Query: 121 LLNN-FPALKLSGYPVETSKVTSFTPYEMSEYVSDVDLAEKETVSFEDMQKLVSTGDIKN 179
LLN A K +G P + S VT + + + LA+ E + F + +
Sbjct: 51 LLNGGLDAWKAAGGPTDNSVVTPKLGNVNVKSLDNTILAKFEEIPFNEFAE-------NK 103
Query: 180 YQVLDARSLQRFTGEAPE----PRPGLSSGHIPGVQPLPFLEVLNSDKTFTSDREEMLKK 235
LDAR+ +FTGE P P + H+ G P +V+ D + ++++ +
Sbjct: 104 VDYLDARTTPQFTGEEPLNKTFPDTKATGSHVTGAISFPMAKVIGPDGFIS--QQDVDAQ 161
Query: 236 IQDFVSQTGNEFETDKPTIAMCGTGVTGVIIKTALEISGVKNVRLYDGSWTEWVMKDGEV 295
I + ++GN+ C TG+ +I ALE SG+K +LY+GS TE + E+
Sbjct: 162 IANLGLKSGNQ------VYVACNTGIQASVIFVALERSGIK-AKLYNGSMTELASRAPEL 214
Query: 296 AKGS 299
+
Sbjct: 215 VNAT 218
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.315 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 18,627,292
Number of Sequences: 60738
Number of extensions: 822622
Number of successful extensions: 1755
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1729
Number of HSP's gapped (non-prelim): 9
length of query: 299
length of database: 30,389,216
effective HSP length: 106
effective length of query: 193
effective length of database: 23,950,988
effective search space: 4622540684
effective search space used: 4622540684
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)