ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2461 good A KOG2749 RNA processing and modification mRNA cleavage and polyadenylation factor IA/II complex, subunit CLP1
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2461 861625 862935 437
(437 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOR250c [A] KOG2749 mRNA cleavage and polyadenylation factor IA/... 407 e-113
SPAC22H10.05c [A] KOG2749 mRNA cleavage and polyadenylation fact... 177 4e-44
At3g04680 [A] KOG2749 mRNA cleavage and polyadenylation factor I... 136 5e-32
At5g39930 [A] KOG2749 mRNA cleavage and polyadenylation factor I... 115 1e-25
Hs5803029 [A] KOG2749 mRNA cleavage and polyadenylation factor I... 114 3e-25
CE17941 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/... 112 1e-24
7303312 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/... 102 1e-21
>YOR250c [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 445
Score = 407 bits (1046), Expect = e-113
Identities = 211/425 (49%), Positives = 293/425 (68%), Gaps = 12/425 (2%)
Query: 18 LPQGHEWTVQVPAGSKLTVIVKYGIAEILGTELANDVPYTFQGVIVNIYAIERSMIEWKC 77
+P+G +W + + A KL V V GI EI GTELA D YTFQ IYA+E + + WKC
Sbjct: 28 IPKGSDWQIDLKAEGKLIVKVNSGIVEIFGTELAVDDEYTFQNWKFPIYAVEETELLWKC 87
Query: 78 LEELEPKVSENKSYHA--YIYNLNFALERLRLSSFNGPRVLIVGNASTGKSSLAQMLCSY 135
+L K H YIYNL+F LE++R+S+F GPRV+IVG + TGK+SL++ LCSY
Sbjct: 88 -PDLTTNTITVKPNHTMKYIYNLHFMLEKIRMSNFEGPRVVIVGGSQTGKTSLSRTLCSY 146
Query: 136 ALKIRHYQPLLVNLNPQDGVFSLPGSITATPISELLDVESSIWGQSITTGATKLHNKQPL 195
ALK YQPL +NL+PQ +F++PG I+ATPIS++LD + WGQS+T+GAT LHNKQP+
Sbjct: 147 ALKFNAYQPLYINLDPQQPIFTVPGCISATPISDILDAQLPTWGQSLTSGATLLHNKQPM 206
Query: 196 VKNYGLENIRDNRPLYLSTITQLASGVQQRLKNDPIVRRSGVIIDTPKLSHLDTEDWSEV 255
VKN+GLE I +N+ LYL I+QL V QRL DP VRRSG I+DTP +S LD E+ +E+
Sbjct: 207 VKNFGLERINENKDLYLECISQLGQVVGQRLHLDPQVRRSGCIVDTPSISQLD-ENLAEL 265
Query: 256 GHMIEQFDIN-AIVVCAESDDLAIELSEVFRTKIG--SIVRIPPPGSIIAIDDIMRRALQ 312
H+IE+ ++N +V+C+E+D L ++ + F ++G +I IP + A+DD+ +R+LQ
Sbjct: 266 HHIIEKLNVNIMLVLCSETDPLWEKVKKTFGPELGNNNIFFIPKLDGVSAVDDVYKRSLQ 325
Query: 313 RVQIREYFYGTTETVLSPYTMGAGFEELTVFRPRNISEYGEDKKDVDLTVFDKIEVNSSN 372
R IREYFYG+ +T LSPY +G +E+LT+++P N+ + +V + + SN
Sbjct: 326 RTSIREYFYGSLDTALSPYAIGVDYEDLTIWKPSNVFD-----NEVGRVELFPVTITPSN 380
Query: 373 LQHAIIAITNISRKESQDKLNTASIIGYGLITEVNDSKKKLRVLLPVPSRIPDRAMILTE 432
LQHAIIAIT R+ Q + + I+G+ LITEVN+ ++KLRVLLPVP R+P +AMILT
Sbjct: 381 LQHAIIAITFAERRADQATVIKSPILGFALITEVNEKRRKLRVLLPVPGRLPSKAMILTS 440
Query: 433 YRYLE 437
YRYLE
Sbjct: 441 YRYLE 445
>SPAC22H10.05c [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 456
Score = 177 bits (448), Expect = 4e-44
Identities = 140/457 (30%), Positives = 233/457 (50%), Gaps = 43/457 (9%)
Query: 18 LPQGHEWTVQVPAGSKLTVIVKYGIAEILGTELANDVPYTFQGVIVNIYAIERSMIEWKC 77
+P+ EW +V + + + + G AE GTELA PY F V IY + +E +
Sbjct: 6 IPKECEWRFEVDEPA-IQIRLVSGNAEYFGTELALGPPYHFTRVKGAIYTWQGCTLEVEG 64
Query: 78 LEELEPKVSENKSYHAYIYNLNFALERLRLSSFN---------GPRVLIVGNASTGKSSL 128
+E V+E Y+ NL+FALE LRL + N GP V ++G S GK+S+
Sbjct: 65 EPSVE-YVAEETPMSTYL-NLHFALEGLRLQAENAAANDESSYGPCVCLIGPRSCGKTSV 122
Query: 129 AQMLCSYALKIRHYQPLLVNLNPQDGVFSLPGSITATPISELLDVESSI-WGQSITTGAT 187
++L SYALK + P+ VNL+P + +LPGSI+A + +LD++ + +G S +TG T
Sbjct: 123 LKILESYALKQSRH-PICVNLDPTQPMLALPGSISAFHNATILDIQDADGFGASTSTGPT 181
Query: 188 KLHNKQPLVKNYGLENIRDNRPLYLSTITQLASGVQQRLKNDPIVRRSGVIIDTPKLSHL 247
+ K PLV N+GL++ DN LY ++++LA V R+ R SG ++DT +
Sbjct: 182 HVLAKVPLVYNFGLDSPLDNPKLYKLSLSRLALAVHSRMSQSKDARVSGCLVDTSSIQE- 240
Query: 248 DTEDWSEVGH-MIEQFDINAIVVCAESDDLAIELSEVFRTKIG-SIVRIPPPGSIIAIDD 305
+ E + ++ H +I F IN I+V S+ L + + S+V++ G I ++
Sbjct: 241 NAEKYQDILHSIITDFRINIIIVLG-SERLYSSMKRKYADATWLSVVKVSSSGGCIDREE 299
Query: 306 IMRRALQRVQIREYFYGTTETVLSPYTMGAGFEELTVFRPRNISEYGEDKKDVDL----- 360
+ Q I++YFYG LSP +M +L V+R SE G + L
Sbjct: 300 EWIQQFQARCIKQYFYGDDRMPLSPLSMIVDSTQLVVYRVLEASESGPKSSVLPLGFEEE 359
Query: 361 -TVFDKIEVNSS-------------------NLQHAIIAITNISRKESQDKLNTASIIGY 400
T +K + N+S LQ++I+A++++ E + + + IIGY
Sbjct: 360 NTQSEKQDGNTSLRLHGKGEFLERISTEAMTILQNSILAVSSVGEDEDEATVVDSCIIGY 419
Query: 401 GLITEVNDSKKKLRVLLPVPSRIPDRAMILTEYRYLE 437
+++V+D K ++ +L PVP ++P A+I+ ++ E
Sbjct: 420 VFVSDVDDVKNRMTLLSPVPEQLPSNALIMGTCKWQE 456
>At3g04680 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 444
Score = 136 bits (343), Expect = 5e-32
Identities = 121/447 (27%), Positives = 219/447 (48%), Gaps = 43/447 (9%)
Query: 10 SETTKTSSLPQGHEWTVQVPAGSKLTVIVKYGIAEILGTELANDVPYTFQGVI-VNIYAI 68
S K L + E ++V + L + V G AEI G+EL ++ TF + ++
Sbjct: 20 SANLKQVKLERESELRIEV-SEEPLRLRVVNGTAEIFGSELPPEIWRTFPPRMKFAVFTW 78
Query: 69 ERSMIEWKCLEELEPKVSENKSYHAYIYNLNFALERLRL----------SSFNGPRVLIV 118
+ IE + E + E +YI N++ L+ R S GPRV++V
Sbjct: 79 YGATIEMDGVTETDYTADETPMV-SYI-NVHAILDARRRFAKASTSNDPESSQGPRVIVV 136
Query: 119 GNASTGKSSLAQMLCSYALKIRHYQPLLVNLNPQDGVFSLPGSITATPISELLDVESSIW 178
G +GKS+L +ML S+A K + ++P V+L+ G ++PGSI A PI LD +
Sbjct: 137 GPTDSGKSTLTKMLLSWAAK-QGWRPTFVDLDVGQGSITIPGSIAAAPIEMPLD---PVE 192
Query: 179 GQSITTGATKLHNKQPLVKNYGLENIRDNRPLYLSTITQLASGVQQRLKNDPIVRRSGVI 238
G + LV YG + N LY + + +LA ++++ +P R +G++
Sbjct: 193 GFPLDMA---------LVYYYGHASPNMNVELYKALVKELAQVLEKQFVGNPESRAAGMV 243
Query: 239 IDTPKLSHLDTEDWSEVGHMIEQFDINAIVVCAESDDLAIELSEVFRTKIG-SIVRIPPP 297
I+T + ++ + + H I+ F+ + ++V + + L L +V R+K +V++
Sbjct: 244 INT--MGWIEGIGYELLLHAIDTFNASVVLVLGQ-EKLFSRLKDVLRSKSNVDVVKLHKS 300
Query: 298 GSIIAIDDIMRRALQRVQIREYFYGTTETVLSPYTMGAGFEELTVFR-------PRNISE 350
G ++A +R+ + +I+EYFYG ++ LSPY + F +L VFR P++
Sbjct: 301 GGVVARVKEVRKRSRNFKIQEYFYGLSKE-LSPYANTSSFSDLQVFRIGGGPQAPKSALP 359
Query: 351 YGEDKKDVDLTVFDKIEVNSSNLQHAIIAITNISRKESQDKLNTASIIGYGLITEVNDSK 410
G L V + ++ +L H+++A+ S E D++ ++++ G+ +TEVN K
Sbjct: 360 AGSTSVSNPLRV-TPVNIDDRDLLHSVLAV---SYAEEPDQIISSNVSGFVYVTEVNVQK 415
Query: 411 KKLRVLLPVPSRIPDRAMILTEYRYLE 437
KK+ L P P +P + ++ +LE
Sbjct: 416 KKITYLAPSPGTLPSKLLVAGSLAWLE 442
>At5g39930 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 423
Score = 115 bits (289), Expect = 1e-25
Identities = 103/430 (23%), Positives = 201/430 (45%), Gaps = 42/430 (9%)
Query: 18 LPQGHEWTVQVPAGSKLTVIVKYGIAEILGTELANDVPYTFQGVIV-NIYAIERSMIEWK 76
L + E +++ S L + + G AEI G EL ++V TF ++ ++ + IE
Sbjct: 11 LEKQSELRIELQPTSPLRLRLLDGKAEIFGYELPHEVWITFPPLMTFAVFTWYGATIEID 70
Query: 77 CLEELEPKVSENKSYHAYIYNLNFALERLRLSSFN-------GPRVLIVGNASTGKSSLA 129
+ E E + + + ++R R++S GPRV+IVG+ +GKS+LA
Sbjct: 71 GITGNEYISCETPMVNYLGLHNSLQVQRHRVTSSTRDSASSQGPRVIIVGDIDSGKSTLA 130
Query: 130 QMLCSYALKIRHYQPLLVNLNPQDGVFSLPGSITATPISELLDVESSIWGQSITTGATKL 189
+ML ++A+K ++P V+LN ++PG+I A PI L+D + G +
Sbjct: 131 KMLLNWAVK-DGWKPTFVDLNVGQSSITIPGTIAAAPIKMLVD---PVEGFPL------- 179
Query: 190 HNKQPLVKNYGLENIRDNRPLYLSTITQLASGVQQRLKNDPIVRRSGVIIDTPKLSHLDT 249
+ L+ +GL N N LY + + +LA +++ + R SG++IDT + +
Sbjct: 180 --DKALIHYFGLTNPSVNLRLYRTLVEELARELKEEFSANAESRASGMVIDT--MGFIVR 235
Query: 250 EDWSEVGHMIEQFDINAIVVCAESDDLAIELSEVFRTKIG-SIVRIPPPGSIIAIDDIMR 308
E ++ + H I F+ + ++V + + L +L + + K ++ + + + R
Sbjct: 236 EGYALLLHAIRTFNASLVIVVGQEEKLVYDLKKNLKFKKNLQVLNLEKSEGVFSRSSDFR 295
Query: 309 RALQRVQIREYFYGTTETVLSPYTMGAGFEELTVFR--------PRNISEYGEDKKDVDL 360
+ L+ I+ YFYG T L+ YT F ++ V+R + + G D + L
Sbjct: 296 KTLRNSNIQNYFYGVTND-LTVYTKTVKFSDVQVYRIGDFRVSGSTSAHQRGNDPLKITL 354
Query: 361 TVFDKIEVNSSNLQHAIIAITNISRKESQDKLNTASIIGYGLITEVNDSKKKLRVLLPVP 420
D +H + + IS D++ ++ + G+ I V+ S++++ + P
Sbjct: 355 VTID---------EHLVNKVLAISYAIKPDQIISSIVAGFVCIKNVDISEERITYVSPSA 405
Query: 421 SRIPDRAMIL 430
+ +P + +IL
Sbjct: 406 AELPSKILIL 415
>Hs5803029 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 425
Score = 114 bits (285), Expect = 3e-25
Identities = 107/447 (23%), Positives = 207/447 (45%), Gaps = 41/447 (9%)
Query: 5 EFSEVSETTKTSSLPQGHEWTVQVPAGSKLTVIVKYGIAEILGTELANDVPYTFQ-GVIV 63
E ++ + T L + E +V A + + + G+AEI GTEL + +TF G V
Sbjct: 4 EANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKV 63
Query: 64 NIYAIERSMIEWKCLEELEPKVSENKSYHAYIYNLNFALERLRLSSFN----GPRVLIVG 119
++ ++ E+ VS++ Y+ N + ALE++R + GPRV++VG
Sbjct: 64 AVFTWHGCSVQLSGRTEVA-YVSKDTPMLLYL-NTHTALEQMRRQAEKEEERGPRVMVVG 121
Query: 120 NASTGKSSLAQMLCSYALKIRHYQPLLVNLNPQDGVFSLPGSITATPISELLDVESSIWG 179
GKS++ ++L +YA+++ +P V L+ G S+PG++ A I DVE G
Sbjct: 122 PTDVGKSTVCRLLLNYAVRLGR-RPTYVELDVGQGSVSIPGTMGALYIERPADVEE---G 177
Query: 180 QSITTGATKLHNKQPLVKNYGLENIRDNRPLYLSTITQLASGVQQRLKNDPIVRRSGVII 239
SI + PLV ++G N LY ++LA QR + + SG +I
Sbjct: 178 FSI---------QAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVI 228
Query: 240 DTPKLSHLDTEDWSEVGHMIEQFDINAIVVCAES---DDLAIELSEVFRTKIGSIVRIPP 296
+T + + + H F+++ +VV + ++L +L RT V +P
Sbjct: 229 NT--CGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRT-----VLLPK 281
Query: 297 PGSIIAIDDIMRRALQRVQIREYFYGTTETVLSPYTMGAGFEELTVFR------PRNISE 350
G ++ RR + +IREYFYG P+ F ++ +++ P +
Sbjct: 282 SGGVVERSKDFRRECRDERIREYFYG-FRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLP 340
Query: 351 YGEDKKDVDLTVFDKIEVNSSNLQHAIIAITNISRKESQDKLNTASIIGYGLITEVNDSK 410
G ++D L + ++ H +++++ + + +++ L+ S+ G+ ++T V+
Sbjct: 341 LGMSQEDNQLKLVP--VTPGRDMVHHLLSVS--TAEGTEENLSETSVAGFIVVTSVDLEH 396
Query: 411 KKLRVLLPVPSRIPDRAMILTEYRYLE 437
+ VL P P +P +++ + R+++
Sbjct: 397 QVFTVLSPAPRPLPKNFLLIMDIRFMD 423
>CE17941 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 428
Score = 112 bits (279), Expect = 1e-24
Identities = 95/417 (22%), Positives = 197/417 (46%), Gaps = 47/417 (11%)
Query: 41 GIAEILGTELANDVPYTFQGVI-VNIYAIERSMIEWKCLEELEPKVSENKSYHAYIYNLN 99
G AEI GTEL + YTF V + + + IE E V+E+ Y+ N++
Sbjct: 34 GYAEIFGTELLLNKKYTFPAKSRVAAFTWKGATIELVGTTE-SAYVAESTPMVIYL-NIH 91
Query: 100 FALERLRL----------SSFNGPRVLIVGNASTGKSSLAQMLCSYALKIRHYQPLLVNL 149
A+E +R + GPR+L+VG GK++++++LC+Y+++ + P+ V L
Sbjct: 92 AAMEEVRKKREEQAAGNSNKAKGPRLLLVGPTDVGKTTVSRILCNYSVR-QGRTPIFVEL 150
Query: 150 NPQDGVFSLPGSITATPISELLDVESSIWGQSITTGATKLHNKQPLVKNYGLENIRDNRP 209
+ S+PG++ A + + DV QP+V N+G + N
Sbjct: 151 DVGQNSVSVPGTVAAVLVQKTADVIDG------------FERNQPIVFNFGHTSPSANLS 198
Query: 210 LYLSTITQLASGVQQRLKNDPIVRRSGVIIDTPKLSHLDTEDWSEVGHMIEQFDINAIVV 269
LY + ++A+ + +++ + + G+II+T +D E + + F+++ ++V
Sbjct: 199 LYEALFKEMATTLNAQIQENDEAKIGGMIINT--CGWVDGEGYKCIVKAASAFEVDVVIV 256
Query: 270 CAES---DDLAIELSEVFRTKIGSIVRIPPPGSIIAIDDIMRRALQRVQIREYFYGTTET 326
DL+ EL E R + +P G + +R ++ + YFYGT
Sbjct: 257 LDHERLYSDLSKELPEFVR-----LTHVPKSGGVEQRTGQIRSKMRGENVHRYFYGTRAN 311
Query: 327 VLSPYTMGAGFEELTVFR------PRNISEYGEDKKDVDLTVFDKIEVNSSNLQHAIIAI 380
L P+T F+++T+ + P + +G + ++ + + I S++++H + A
Sbjct: 312 NLYPFTFDVSFDDVTLCKIGAEQLPDSCLPFGMEVENHETKLV--IMEPSADIKHHLFAF 369
Query: 381 TNISRKESQDKLNTASIIGYGLITEVNDSKKKLRVLLPVPSRIPDRAMILTEYRYLE 437
+ ++ + + + + + G+ L+TEV+ K+ + +L P IP + ++ ++ +L+
Sbjct: 370 SRSTK--ADENVLKSPVFGFCLVTEVDLEKRTMSILCP-QRTIPSKVLVFSDITHLD 423
>7303312 [A] KOG2749 mRNA cleavage and polyadenylation factor IA/II complex
subunit CLP1
Length = 423
Score = 102 bits (253), Expect = 1e-21
Identities = 98/421 (23%), Positives = 195/421 (46%), Gaps = 41/421 (9%)
Query: 32 SKLTVIVKYGIAEILGTELANDVPYTFQ-GVIVNIYAIERSMIEWKCLEELEPKVSENKS 90
+K+ V + G AE+ GTEL Y F G V I+ + ++ ++ +S+
Sbjct: 26 AKVLVSLVSGFAELFGTELVKKKQYEFGVGAKVAIFTYQGCVLHVSGKMDV-CYISKETP 84
Query: 91 YHAYIYNLNFALERLRLSSFN----GPRVLIVGNASTGKSSLAQMLCSYALKIRHYQPLL 146
Y+ N + ALE+ R+ + GP ++VG GKS+L ++L +YA+++ +PL
Sbjct: 85 MVQYV-NCHAALEQFRMEAEEKDRYGPVAMVVGPMDVGKSTLCRILLNYAVRVGR-RPLY 142
Query: 147 VNLNPQDGVFSLPGSITATPISELLDVESSIWGQSITTGATKLHNKQPLVKNYGLENIRD 206
+L+ G ++ GS+ I +VE PLV ++G ++
Sbjct: 143 ADLDVGQGSIAISGSVATILIERPANVEEGF------------AKTAPLVYHFGHKSPSG 190
Query: 207 NRPLYLSTITQLASGVQQRLKNDPIVRRSGVIIDTPKLSHLDTEDWSEVGHMIEQFDINA 266
N LY + ++++A Q L ++ + SG+II+T + ++ + H + + A
Sbjct: 191 NSVLYNAVVSKMAEVTLQSLNSNKRTKSSGIIINT--CGWVKGSGYAHLLHAAKAYGACA 248
Query: 267 IVVCAESDDLAIELSEVFRTKIGSIVRIPPPGSIIAIDDIMRRALQRVQIREYFYGTTET 326
I V + L +V K +V +P G ++ +R + +I+EYFYG T
Sbjct: 249 IFVLDQERLYNELLRDV--PKGVHVVLLPKSGGVVERSKELRHEARDQRIKEYFYGNTRA 306
Query: 327 VLSPYTMGAGFEELTVFR------PRNISEYG---EDKKDVDLTVFDKIEVNSSNLQHAI 377
P++ F++L +++ P + G ED K + V + L H +
Sbjct: 307 PFYPFSFEVKFQDLRLYKIGAPPLPDSCMPIGMKAEDNKTKVVAV-----TPTPALIHHV 361
Query: 378 IAITNISRKESQDKLNTASIIGYGLITEVNDSKKKLRVLLPVPSRIPDRAMIL-TEYRYL 436
+A++ + +D + ++ G+ +TEV+ ++ + +L P P +P A++L +E +++
Sbjct: 362 LALS--FAESVEDDVIGTNVAGFCCVTEVDMERQAVMLLSPQPRPLPPNALLLWSELQFM 419
Query: 437 E 437
+
Sbjct: 420 D 420
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.135 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 25,157,024
Number of Sequences: 60738
Number of extensions: 1041331
Number of successful extensions: 3381
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3352
Number of HSP's gapped (non-prelim): 7
length of query: 437
length of database: 30,389,216
effective HSP length: 109
effective length of query: 328
effective length of database: 23,768,774
effective search space: 7796157872
effective search space used: 7796157872
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)