ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2539 check: MH I KOG0874 Lipid transport and metabolism Sphingolipid hydroxylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2539 893059 892043 -339
(339 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR297w [I] KOG0874 Sphingolipid hydroxylase 431 e-121
SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase 238 7e-63
At1g69640 [I] KOG0874 Sphingolipid hydroxylase 204 1e-52
At1g14290 [I] KOG0874 Sphingolipid hydroxylase 197 2e-50
Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase 87 3e-17
SPAC630.08c [I] KOG0873 C-4 sterol methyl oxidase 77 5e-14
YGR060w [I] KOG0873 C-4 sterol methyl oxidase 74 3e-13
CE03386 [I] KOG0873 C-4 sterol methyl oxidase 72 9e-13
At2g29390 [I] KOG0873 C-4 sterol methyl oxidase 68 2e-11
At1g07420 [I] KOG0873 C-4 sterol methyl oxidase 68 2e-11
7292910 [I] KOG0873 C-4 sterol methyl oxidase 67 4e-11
At4g12110 [I] KOG0873 C-4 sterol methyl oxidase 64 4e-10
At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase 62 2e-09
Hs5902070 [I] KOG0872 Sterol C5 desaturase 61 3e-09
Hs20558831 [I] KOG0872 Sterol C5 desaturase 61 3e-09
Hs4502499 [I] KOG0873 C-4 sterol methyl oxidase 57 4e-08
CE04497 [I] KOG0873 C-4 sterol methyl oxidase 57 4e-08
7292879 [I] KOG0873 C-4 sterol methyl oxidase 57 5e-08
YLR056w [I] KOG0872 Sterol C5 desaturase 55 1e-07
At3g02580 [I] KOG0872 Sterol C5 desaturase 52 2e-06
>YDR297w [I] KOG0874 Sphingolipid hydroxylase
Length = 349
Score = 431 bits (1109), Expect = e-121
Identities = 203/312 (65%), Positives = 239/312 (76%)
Query: 24 FMNSKAPPASVITKKQDILPWISDGKLALALPVVAYWLYSMFFHYIDTNEVFEQYRIHPS 83
FM+ PA + K+ +LP +SDG LAL PVVAYW S FH IDT + E+YRIHPS
Sbjct: 25 FMHYAKAPAINLRPKESLLPEMSDGVLALVAPVVAYWALSGIFHVIDTFHLAEKYRIHPS 84
Query: 84 EEVQQRNKASRLEVLKEVILQHFIQTFTGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVP 143
EEV +RNKASR+ V EVILQH IQT GL ++ +P+ +GFE+ MWK R LP +P
Sbjct: 85 EEVAKRNKASRMHVFLEVILQHIIQTIVGLIFMHFEPIYMTGFEENAMWKLRADLPRIIP 144
Query: 144 SVFIKFMYCYGWSMIRIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGA 203
I + Y YG S ++IF GF VD+WQY+LHR+MHMNKTLYK FHS HH LYVPYAYGA
Sbjct: 145 DAAIYYGYMYGMSALKIFAGFLFVDTWQYFLHRLMHMNKTLYKWFHSVHHELYVPYAYGA 204
Query: 204 LYNSPVEGFLLDTLGTGIAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFS 263
L+N+PVEGFLLDTLGTGIA +T L H EQ++L+TFAT+KTVDDHCGYALPLDPFQWLF
Sbjct: 205 LFNNPVEGFLLDTLGTGIAMTLTHLTHREQIILFTFATMKTVDDHCGYALPLDPFQWLFP 264
Query: 264 NNAVYHDIHHQSFGIKSNFSQPFFTIWDKFCDTKYHGFEEYEKKQRRVTIDKYKEFLNKR 323
NNAVYHDIHHQ FGIK+NF+QPFFT WD T + GFEEY+KKQRRVTIDKYKEFL +R
Sbjct: 265 NNAVYHDIHHQQFGIKTNFAQPFFTFWDNLFQTNFKGFEEYQKKQRRVTIDKYKEFLQER 324
Query: 324 KLEHQAKLKAMK 335
+LE + KLK K
Sbjct: 325 ELEKKEKLKNFK 336
>SPBC887.15c [I] KOG0874 Sphingolipid hydroxylase
Length = 293
Score = 238 bits (608), Expect = 7e-63
Identities = 118/271 (43%), Positives = 160/271 (58%), Gaps = 12/271 (4%)
Query: 50 LALALPVVAYWLYSMFF---HYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQHF 106
++L PV+ YW+ S FF HYI+ VFE+YRIHP EE+ +RN+ ++ V+K V+ Q
Sbjct: 16 VSLVSPVIIYWVASAFFGFLHYIEL-PVFEKYRIHPPEEIARRNRVPQMAVVKAVLFQQL 74
Query: 107 IQTFTGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFI------KFMYCYGWSMIRI 160
+ G+ + + P E +M ++ +P++ K Y + +
Sbjct: 75 CEVVVGIALAMFEGYPEPIDEAKQMLRYEAFFSKNLPALLQVAPFAPKLAYNFIVPAFQY 134
Query: 161 FIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTG 220
F F I+DSWQY+ HR +H NK LY H+ HHRL VPYA GALYN P EG +LDT G G
Sbjct: 135 FFAFFIIDSWQYFWHRYLHYNKKLYNMIHAHHHRLQVPYAMGALYNHPFEGLILDTFGAG 194
Query: 221 IAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSFGIKS 280
+A + L+ + V+ +T +TLKTVDDHCGY P DP Q F+NNA YHD+HHQ +G +
Sbjct: 195 VAYLAAGLSPQQAVIFFTLSTLKTVDDHCGYVFPYDPLQMFFANNARYHDLHHQPYGFQK 254
Query: 281 NFSQPFFTIWDKFCDTKYHGFEE--YEKKQR 309
NFSQPFFT WD T E YEKKQ+
Sbjct: 255 NFSQPFFTFWDHVLGTYMPPKSETPYEKKQK 285
>At1g69640 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 204 bits (519), Expect = 1e-52
Identities = 101/252 (40%), Positives = 148/252 (58%), Gaps = 23/252 (9%)
Query: 45 ISDGKLALALPVVAYWLYSMFFHYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQ 104
+SD L P+V YWLYS + + + E YR+H E +++N S+ V+K V++Q
Sbjct: 5 VSDELLGTVAPIVVYWLYSGIYVALSS---LESYRLHSKVEEEEKNLVSKSSVVKGVLVQ 61
Query: 105 HFIQTFTGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGF 164
+Q + +L+ + +K + + + FV ++ +
Sbjct: 62 QVVQAVVAI-LLFTVTGSDAEADKAQQFSFLVLARQFVTAMIV----------------- 103
Query: 165 CIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAI 224
+D+WQY++HR MH NK LYK HS+HHRL VPYAYGALYN PVEG LLDT+G ++ +
Sbjct: 104 --LDTWQYFMHRYMHQNKFLYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFL 161
Query: 225 VTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSFGIKSNFSQ 284
V+ ++ + ++FAT+KTVDDHCG LP + F +F NN+ YHDIHHQ +G K NFSQ
Sbjct: 162 VSGMSPRTSIFFFSFATIKTVDDHCGLWLPGNLFHMVFKNNSAYHDIHHQLYGTKYNFSQ 221
Query: 285 PFFTIWDKFCDT 296
PFF +WD+ T
Sbjct: 222 PFFVMWDRILGT 233
>At1g14290 [I] KOG0874 Sphingolipid hydroxylase
Length = 258
Score = 197 bits (500), Expect = 2e-50
Identities = 97/257 (37%), Positives = 146/257 (56%), Gaps = 33/257 (12%)
Query: 45 ISDGKLALALPVVAYWLYSMFFHYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQ 104
ISD L +P++ YW+YS + + + ++YR+H + ++N S+ V+K V+LQ
Sbjct: 5 ISDEFLGTFVPILVYWVYSGMYICLGS---LDKYRLHSKIDEDEKNLVSKSAVVKGVLLQ 61
Query: 105 HFIQTFTGLGMLYL-----DPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIR 159
+Q + + + D T F + + +
Sbjct: 62 QTLQAIISVILFKITGSDADAATTQQFSILLLAR-------------------------Q 96
Query: 160 IFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGT 219
I ++D+WQY++HR MH+NK LYK HS+HHRL VPY+YGALYN P+EG LLDT+G
Sbjct: 97 FIIAMLVIDTWQYFIHRYMHLNKFLYKHIHSQHHRLIVPYSYGALYNHPLEGLLLDTIGG 156
Query: 220 GIAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSFGIK 279
++ + + ++ + ++FAT+KTVDDHCG LP +PF FSNN+ YHD+HHQ +G K
Sbjct: 157 ALSFLFSGMSPRTAIFFFSFATIKTVDDHCGLWLPGNPFHIFFSNNSAYHDVHHQLYGTK 216
Query: 280 SNFSQPFFTIWDKFCDT 296
NFSQPFF +WD+ T
Sbjct: 217 YNFSQPFFVMWDRILGT 233
>Hs5803157 [I] KOG0873 C-4 sterol methyl oxidase
Length = 293
Score = 87.4 bits (215), Expect = 3e-17
Identities = 57/178 (32%), Positives = 92/178 (51%), Gaps = 24/178 (13%)
Query: 142 VPSVFIKFMYCYGWSMIRIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAY 201
+P + C+G ++I D+W Y+LHR++H +K +YK H HH P+
Sbjct: 132 MPRWYFLLARCFGCAVIE--------DTWHYFLHRLLH-HKRIYKYIHKVHHEFQAPFGM 182
Query: 202 GALYNSPVEGFLLDTLGTG-IAAIVTQLNHTEQVVLYTFAT---LKTVDDHCGYALPLDP 257
A Y P+E + LGTG IV +H ++L+ + T L+T+D H GY +PL+P
Sbjct: 183 EAEYAHPLETLI---LGTGFFIGIVLLCDHV--ILLWAWVTIRLLETIDVHSGYDIPLNP 237
Query: 258 FQWL-FSNNAVYHDIHHQSFGIKSNFSQPFFTIWDKF--CDTKYHGFEEYEKKQRRVT 312
+ F + +HD HH +F N++ FT WD+ D++Y+ + E KK + T
Sbjct: 238 LNLIPFYAGSRHHDFHHMNF--IGNYAST-FTWWDRIFGTDSQYNAYNEKRKKFEKKT 292
>SPAC630.08c [I] KOG0873 C-4 sterol methyl oxidase
Length = 300
Score = 76.6 bits (187), Expect = 5e-14
Identities = 81/289 (28%), Positives = 122/289 (42%), Gaps = 43/289 (14%)
Query: 45 ISDGKLALALPVVAYWLYSMFFHYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQ 104
++ G ++ L + Y+ + + ID F +++I P + A + E + V+L
Sbjct: 46 VATGLMSFLLHELIYFGRCIPWMIIDAMPYFRRWKIQPKKVP---TLAEQWECTRLVLLS 102
Query: 105 HFIQTFTGLGMLYLDPV-PTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIG 163
HF + + DP+ T G S+PF + I W +I +
Sbjct: 103 HFTVELPQIWLF--DPMCATFGLST--------SVPFPPVTKMI-------W---QITLF 142
Query: 164 FCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAA 223
F + D+W YW HR+ H Y+ H HHR P+ A Y P+E LL +
Sbjct: 143 FFLEDTWHYWAHRLFHYG-IFYRFIHKVHHRYSAPFGLSAEYAHPLEIILLGAGTVFVPL 201
Query: 224 IVTQLNH-TEQVVLYTFATLK---TVDDHCGYALP--LDPFQWLFSNNAVYHDIHHQSFG 277
+ H V +Y + TL+ VD H GY P L+ F +++ A +HD HH +F
Sbjct: 202 MWCYFTHDLHLVTMYIWITLRLFQAVDSHAGYDFPWSLNKFLPIWA-GADHHDYHHMAF- 259
Query: 278 IKSNFSQPFFTIWDKF--CDTKYHGFEEYEKKQRRVTIDKYKEFLNKRK 324
K NFS F WD D YH F K RR+ KY+ K K
Sbjct: 260 -KDNFSSS-FRWWDAVLKTDQNYHQF-----KARRLAA-KYEAESKKAK 300
>YGR060w [I] KOG0873 C-4 sterol methyl oxidase
Length = 309
Score = 73.9 bits (180), Expect = 3e-13
Identities = 74/293 (25%), Positives = 125/293 (42%), Gaps = 42/293 (14%)
Query: 45 ISDGKLALALPVVAYWLYSMFFHYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQ 104
++ G + L Y+ + + ID F ++++ P++ + + L LK V+L
Sbjct: 52 LATGLMFFLLHEFMYFFRCLPWFIIDQIPYFRRWKLQPTKIPSAKEQ---LYCLKSVLLS 108
Query: 105 HFIQTFTGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGF 164
HF+ ++ +P F + M + + + VP +K M + I + F
Sbjct: 109 HFL----------VEAIPIWTF--HPMCE-KLGITVEVPFPSLKTM------ALEIGLFF 149
Query: 165 CIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLL--DTLGTGIA 222
+ D+W YW HR+ H YK H +HHR P+ A Y P E L T+G I
Sbjct: 150 VLEDTWHYWAHRLFHYG-VFYKYIHKQHHRYAAPFGLSAEYAHPAETLSLGFGTVGMPIL 208
Query: 223 AIV--TQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWL-FSNNAVYHDIHHQSFGIK 279
++ +L+ V T + VD H GY P + + F A +HD+HH F
Sbjct: 209 YVMYTGKLHLFTLCVWITLRLFQAVDSHSGYDFPWSLNKIMPFWAGAEHHDLHHHYF--I 266
Query: 280 SNFSQPFFTIWDKFCDTKYHGFEEYEKKQRRVTIDKYKEFLNKRKLEHQAKLK 332
N++ F WD DT+ G E ++ R+ K++ E+ A+ K
Sbjct: 267 GNYASS-FRWWDYCLDTE-SGPEAKASREERM----------KKRAENNAQKK 307
>CE03386 [I] KOG0873 C-4 sterol methyl oxidase
Length = 286
Score = 72.4 bits (176), Expect = 9e-13
Identities = 59/227 (25%), Positives = 101/227 (43%), Gaps = 34/227 (14%)
Query: 53 ALPVVAYWLYSMFFHYIDTNEV--FEQYRIHPSEEVQQRNKASRLEVLKEVILQHFIQTF 110
A+ +++WLY++FF ID + + Y+I +E ++ + + L +LK V
Sbjct: 27 AVAGISFWLYNLFFIIIDVTDPKWVQPYKI---QEEKKPSLSKYLSILKVV--------- 74
Query: 111 TGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFM-YCYGWSMIRIFIGFCIVDS 169
G L + P+ T+ + W W +PS +I C +M I GF
Sbjct: 75 -GPNQLIVTPIVTTLWFYVARW-WGMDFGPVIPSWYILLRDACLCMAMDEI--GF----- 125
Query: 170 WQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQLN 229
Y+ HR+ H K LYK H +HH P + ++Y P+E + + + A++ + +
Sbjct: 126 --YYTHRLFHHPK-LYKHIHKKHHEWNAPVSISSIYAHPLEHAISNLSPIYLGAVLFRCH 182
Query: 230 HTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSF 276
+ ++A L T H GY P F +A +HD HH+ F
Sbjct: 183 VVSHYIFTSYAILTTTFHHSGYHFP-------FMLSAEHHDFHHKVF 222
>At2g29390 [I] KOG0873 C-4 sterol methyl oxidase
Length = 253
Score = 68.2 bits (165), Expect = 2e-11
Identities = 49/140 (35%), Positives = 65/140 (46%), Gaps = 14/140 (10%)
Query: 155 WSMI--RIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGF 212
W ++ +I F I D YW HR++H K LYK HS HH P+ + Y P E
Sbjct: 98 WKVVSAQILFYFIIEDFVFYWGHRILH-TKWLYKNVHSVHHEYATPFGLTSEYAHPAEIL 156
Query: 213 LLDTLGTGIAAIV-TQLNHTEQVVLYTFATLK---TVDDHCGYALPLDPFQWL-FSNNAV 267
L G A IV L + L+ + L+ TV+ HCGY P P +L A
Sbjct: 157 FL-----GFATIVGPALTGPHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGAD 211
Query: 268 YHDIHHQSFGIKS-NFSQPF 286
+HD HH+ KS N+S F
Sbjct: 212 FHDYHHRLLYTKSGNYSSTF 231
>At1g07420 [I] KOG0873 C-4 sterol methyl oxidase
Length = 266
Score = 67.8 bits (164), Expect = 2e-11
Identities = 64/241 (26%), Positives = 100/241 (40%), Gaps = 49/241 (20%)
Query: 59 YWLYSMFFHYIDTNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQHFIQTFTGLGMLYL 118
++L + F +++ +Y+I + + A++ + + ++L HF
Sbjct: 34 FFLSGLPFIFLERQGFLSKYKI----QTKNNTPAAQGKCITRLLLYHFSVNL-------- 81
Query: 119 DPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMI--RIFIGFCIVDSWQYWLHR 176
P+ + + + R S P +PS W + +I F I D YW HR
Sbjct: 82 -PLMLASYPVFRAMGMRSSFP--LPS----------WKEVSAQILFYFIIEDFVFYWGHR 128
Query: 177 VMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIV-TQLNHTEQVV 235
++H +K LYK HS HH P+ + Y P E L G A IV L +
Sbjct: 129 ILH-SKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFL-----GFATIVGPALTGPHLIT 182
Query: 236 LYTFATLK---TVDDHCGYALPLDPFQWLFSN------NAVYHDIHHQSFGIKS-NFSQP 285
L+ + L+ TV+ HCGY P W SN A +HD HH+ KS N+S
Sbjct: 183 LWLWMVLRVLETVEAHCGYHFP-----WSLSNFLPLYGGADFHDYHHRLLYTKSGNYSST 237
Query: 286 F 286
F
Sbjct: 238 F 238
>7292910 [I] KOG0873 C-4 sterol methyl oxidase
Length = 278
Score = 67.0 bits (162), Expect = 4e-11
Identities = 63/263 (23%), Positives = 107/263 (39%), Gaps = 35/263 (13%)
Query: 57 VAYWLYSMFFHYID-TNE--VFEQYRIHPSEEVQQRNKASRLEVLKEVILQHFIQTFTGL 113
+ YWLY+ F +D TN +Y+I P + + N A +K V+ + F
Sbjct: 44 IVYWLYAGIFTLMDITNRPRFLRKYKIQPGQN-EPVNLAKLWHAVKVVLFNLTVVNFLVS 102
Query: 114 GMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGFCIVDSWQ-Y 172
++Y + + + LP F S +R + F +++ Y
Sbjct: 103 WVVYEFVYKSENSQDIRV------LPTFKRS-------------LRDLVVFVVLEEIMFY 143
Query: 173 WLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQLNHTE 232
+ HR++H ++++YK H +HH P A LY PVE L + L + + +
Sbjct: 144 YAHRLLH-HRSVYKYVHKKHHEWTAPIAAITLYAHPVEHVLANLLPVATSIAILGTHVAL 202
Query: 233 QVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSFGIKSNFSQPFFTIWDK 292
V++ A + ++ DH GY+ P W + + +HD HH F N++ DK
Sbjct: 203 AYVIFALAIINSMSDHTGYSFP-----W-SAGSVRFHDYHHAKF----NYNYGVLGFLDK 252
Query: 293 FCDTKYHGFEEYEKKQRRVTIDK 315
T E+ QR I K
Sbjct: 253 LHGTYRAPAEQKAPSQRIKKIKK 275
>At4g12110 [I] KOG0873 C-4 sterol methyl oxidase
Length = 298
Score = 63.5 bits (153), Expect = 4e-10
Identities = 56/221 (25%), Positives = 96/221 (43%), Gaps = 22/221 (9%)
Query: 115 MLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGFCIVDSWQYWL 174
+L + P+ + +M + R LP + + + Y F I D YW+
Sbjct: 97 ILVVGPLQLVSYPSIQMIEIRSGLPLPTITEMLSQLVVY----------FLIEDYTNYWV 146
Query: 175 HRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQLNHTEQV 234
HR H +K Y K H HH P Y A Y E LL + T + + +
Sbjct: 147 HRFFH-SKWGYDKIHRVHHEYTAPIGYAAPYAHWAEVLLLG-IPTFMGPAIAPGHMITFW 204
Query: 235 VLYTFATLKTVDDHCGYALPLDPFQWL-FSNNAVYHDIHHQSFG-IKSNFSQPFFTIWDK 292
+ ++ ++ H GY P P +++ F A YHD HH G +SNF+ F
Sbjct: 205 LWIALRQMEAIETHSGYDFPWSPTKYIPFYGGAEYHDYHHYVGGQSQSNFASVF-----T 259
Query: 293 FCDTKYHGFEEYEKKQRRVTIDKYKEFLNKRKLEHQAKLKA 333
+CD Y G ++ + Q+++ +++ KE +K+ +H +K+
Sbjct: 260 YCDYIY-GTDKGYRFQKKL-LEQIKE-SSKKSNKHNGGIKS 297
>At4g22750_1 [I] KOG0873 C-4 sterol methyl oxidase
Length = 524
Score = 61.6 bits (148), Expect = 2e-09
Identities = 66/259 (25%), Positives = 109/259 (41%), Gaps = 33/259 (12%)
Query: 71 TNEVFEQYRIHPSEEVQQRNKASRLEVLKEVILQHFIQTFTGLGMLYLDPVPTSGFEKY- 129
T+++F +Y+I P + +N S + + +++ FI L ++ + +
Sbjct: 65 TSDLFNRYKIQP----KVKNSFSSMFKCYKDVMKMFILVVGPLQLVSYPSIQVDFVFRVL 120
Query: 130 -EMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGFCIVDSWQYWLHRVMHMNKTLYKKF 188
+M + R LP +PS + ++ + F + D YW+HR H K Y+KF
Sbjct: 121 KQMIEIRSGLP--LPSCME--------IVAQLVVYFLVEDYTNYWVHRFFHC-KWGYEKF 169
Query: 189 HSRHHRLYVPYAYGALYNSPVEGFLLDT---LGTGIAAIVTQLNHTEQVVLY-TFATLKT 244
H HH P Y A Y E LL LG IA H L+ ++
Sbjct: 170 HHIHHEYTAPIGYAAPYAHWAEVLLLGIPTFLGPAIAP-----GHMITFWLWIALRQIEA 224
Query: 245 VDDHCGYALPLDPFQWL-FSNNAVYHDIHHQSFG-IKSNFSQPFFTIWDKFCDTKYHGFE 302
++ H GY P +++ F A YHD HH G +SNF+ F +CD Y +
Sbjct: 225 IETHSGYDFPWSLTKYIPFYGGAEYHDYHHYVGGQSQSNFASVF-----TYCDYIYGTDK 279
Query: 303 EYEKKQRRVTIDKYKEFLN 321
Y +++ + K K L+
Sbjct: 280 GYRFQKKLLQQKKVKYSLS 298
Score = 52.4 bits (124), Expect = 1e-06
Identities = 44/146 (30%), Positives = 61/146 (41%), Gaps = 17/146 (11%)
Query: 159 RIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDT-- 216
++ + F I D YW+HR MH K Y+K H HH P Y + Y E +L
Sbjct: 346 QLVVYFLIEDYTNYWIHRWMHC-KWGYEKIHRIHHEYTSPIGYASPYAHWAEILILGIPT 404
Query: 217 -LGTGIAAIVTQLNHTEQVVLY-TFATLKTVDDHCGYALPLDPFQWL-FSNNAVYHDIHH 273
LG IA H L+ + + ++ H GY P + + F YHD HH
Sbjct: 405 FLGPAIAP-----GHIMTFWLWISLRQFEAIETHSGYDFPWSVTKLIPFYGGPEYHDYHH 459
Query: 274 QSFG-IKSNFSQPFFTIWDKFCDTKY 298
G +SNF+ F +CD Y
Sbjct: 460 YVGGQSQSNFASVF-----TYCDYIY 480
>Hs5902070 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 60.8 bits (146), Expect = 3e-09
Identities = 39/128 (30%), Positives = 65/128 (50%), Gaps = 12/128 (9%)
Query: 168 DSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQ 227
D + YW+HR +H ++ +YK+ H HH +P + + P++GF L +L I +
Sbjct: 131 DMFIYWIHRGLH-HRLVYKRLHKPHHIWKIPTPFASHAFHPIDGF-LQSLPYHIYPFIFP 188
Query: 228 LNHTEQVVLYTFATLKTVDDHCG-YALP--LDPFQWLFSNNAVYHDIHHQSFGIKSNFSQ 284
L+ + LY + T+ H G + +P L P F N + +H HH F N+ Q
Sbjct: 189 LHKVVYLSLYILVNIWTISIHDGDFRVPQILQP----FINGSAHHTDHHMFFDY--NYGQ 242
Query: 285 PFFTIWDK 292
+FT+WD+
Sbjct: 243 -YFTLWDR 249
>Hs20558831 [I] KOG0872 Sterol C5 desaturase
Length = 299
Score = 60.8 bits (146), Expect = 3e-09
Identities = 39/128 (30%), Positives = 65/128 (50%), Gaps = 12/128 (9%)
Query: 168 DSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQ 227
D + YW+HR +H ++ +YK+ H HH +P + + P++GF L +L I +
Sbjct: 131 DMFIYWIHRGLH-HRLVYKRLHKPHHIWKIPTPFASHAFHPIDGF-LQSLPYHIYPFIFP 188
Query: 228 LNHTEQVVLYTFATLKTVDDHCG-YALP--LDPFQWLFSNNAVYHDIHHQSFGIKSNFSQ 284
L+ + LY + T+ H G + +P L P F N + +H HH F N+ Q
Sbjct: 189 LHKVVYLSLYILVNIWTISIHDGDFRVPQILQP----FINGSAHHTDHHMFFDY--NYGQ 242
Query: 285 PFFTIWDK 292
+FT+WD+
Sbjct: 243 -YFTLWDR 249
>Hs4502499 [I] KOG0873 C-4 sterol methyl oxidase
Length = 272
Score = 57.0 bits (136), Expect = 4e-08
Identities = 45/146 (30%), Positives = 63/146 (42%), Gaps = 25/146 (17%)
Query: 164 FCIV--DSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGF------LLD 215
FC++ D + H + H LY+ FH HH+ +A Y S E F +++
Sbjct: 130 FCLLLFDMEFFVWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELFSLGFFDMMN 189
Query: 216 TLGTGIAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALP-----LDPFQWLFSNNAVYHD 270
G + T H + L +V+DH GY P L PF W V+HD
Sbjct: 190 VTLLGCHPLTTLTFHVVNIWL-------SVEDHSGYNFPWSTHRLVPFGWY--GGVVHHD 240
Query: 271 IHHQSFGIKSNFSQPFFTIWDKFCDT 296
+HH F NF+ P+FT WDK T
Sbjct: 241 LHHSHF--NCNFA-PYFTHWDKILGT 263
>CE04497 [I] KOG0873 C-4 sterol methyl oxidase
Length = 300
Score = 57.0 bits (136), Expect = 4e-08
Identities = 54/191 (28%), Positives = 77/191 (40%), Gaps = 20/191 (10%)
Query: 131 MWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHS 190
+W LP P+VF S + IF F D +W H + H K LY+ HS
Sbjct: 113 IWVPDTELPILAPTVFEML------SQLAIF--FLAFDFTYFWFHYINHKVKWLYRWCHS 164
Query: 191 RHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQLNHTEQVVLYTFATLKTVDDHCG 250
HH P+A A + P E F + T T I I T + + A + + H G
Sbjct: 165 VHHMYSSPFAASAQHLHPFELFFVGTFITTIPWIFPTHCLTYWIWFF-IAQSVSYEVHIG 223
Query: 251 YALP--LDPFQWLFSNNAVYHDIHHQSFGIKSNFSQPFFTIWDKFCDTKYHGFEEYEKKQ 308
Y P L W +S A HD+HH QP+F D+ G+
Sbjct: 224 YDFPFALHRIFWFYS-GAPAHDMHHLR---PLTCFQPWFNYLDRLM-----GYHITYADL 274
Query: 309 RRVTIDKYKEF 319
+++T K+K+F
Sbjct: 275 KKMTEAKFKKF 285
>7292879 [I] KOG0873 C-4 sterol methyl oxidase
Length = 406
Score = 56.6 bits (135), Expect = 5e-08
Identities = 52/226 (23%), Positives = 93/226 (41%), Gaps = 35/226 (15%)
Query: 56 VVAYWLYSMFFHYID-TNE--VFEQYRIHP--SEEVQQRNKASRLEVLKEVILQHFIQTF 110
++ YW + ++D TN +Y+I P +E V+ R L+V+ V+ Q F
Sbjct: 163 MLVYWTVGTLYTFMDLTNRPACLRKYKIQPGTNEPVEVRRL---LKVIWCVVCN---QIF 216
Query: 111 TGLGMLYLDPVPTSGFEKYEMWKWRQSLPFFVPSVFIKFMYCYGWSMIRIFIGFCIVDSW 170
G+ + Y Y + + R I+ + + W + + + +
Sbjct: 217 VGIPLAYAS---------YRLMEIRGLSD-------IRDLPTFHWVCFELTVCILMEELG 260
Query: 171 QYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDTLGTGIAAIVTQLNH 230
Y+ HR++H +K +YK H +HH P + A+Y P+E + L + + +
Sbjct: 261 FYYSHRLLH-HKHIYKYIHKQHHEWTAPISVTAIYCHPIEHIFSNLLPPFLGVFLMGSHV 319
Query: 231 TEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSF 276
+ + A L T++ H GY LP P + HD HH F
Sbjct: 320 ATAWLWFALAILSTLNAHSGYHLPFFP-------SPEAHDFHHLKF 358
>YLR056w [I] KOG0872 Sterol C5 desaturase
Length = 365
Score = 55.1 bits (131), Expect = 1e-07
Identities = 39/136 (28%), Positives = 61/136 (44%), Gaps = 9/136 (6%)
Query: 157 MIRIFIGFCIVDSWQYWLHRVMHMNKTLYKKFHSRHHRLYVPYAYGALYNSPVEGFLLDT 216
+I F D Y HR +H + +Y+ H HH+ V + + PV+GF L +
Sbjct: 182 IIEYFTFIFFTDCGVYLAHRWLHWPR-VYRALHKPHHKWLVCTPFASHSFHPVDGF-LQS 239
Query: 217 LGTGIAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNNAVYHDIHHQSF 276
+ I ++ L+ ++L+TF TV H G L +P N H +HH F
Sbjct: 240 ISYHIYPLILPLHKVSYLILFTFVNFWTVMIHDGQYLSNNP----AVNGTACHTVHHLYF 295
Query: 277 GIKSNFSQPFFTIWDK 292
N+ Q F T+WD+
Sbjct: 296 NY--NYGQ-FTTLWDR 308
>At3g02580 [I] KOG0872 Sterol C5 desaturase
Length = 281
Score = 51.6 bits (122), Expect = 2e-06
Identities = 41/145 (28%), Positives = 66/145 (45%), Gaps = 19/145 (13%)
Query: 153 YGWSMIRIFIGFCIV--DSWQYWLHRVMHMNKTLYKKFHSRHH-----RLYVPYAYGALY 205
+GW + ++I +V + YW+HR +H K LYK H+ HH P+A A +
Sbjct: 123 FGWILYFVYIAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFH 182
Query: 206 NSPVEGFLLDTLGTGIAAIVTQLNHTEQVVLYTFATLKTVDDHCGYALPLDPFQWLFSNN 265
PV+G +L + IA + ++ T + L + T + H + P
Sbjct: 183 --PVDG-ILQAVPHVIALFIVPIHFTTHIGLLFMEAIWTANIHDCIHGNIWPVM-----G 234
Query: 266 AVYHDIHHQSFGIKSNFSQPFFTIW 290
A YH IHH ++ K N+ +TIW
Sbjct: 235 AGYHTIHHTTY--KHNYGH--YTIW 255
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.326 0.139 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,493,232
Number of Sequences: 60738
Number of extensions: 955634
Number of successful extensions: 3104
Number of sequences better than 1.0e-05: 20
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 16
Number of HSP's that attempted gapping in prelim test: 3064
Number of HSP's gapped (non-prelim): 23
length of query: 339
length of database: 30,389,216
effective HSP length: 107
effective length of query: 232
effective length of database: 23,890,250
effective search space: 5542538000
effective search space used: 5542538000
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)