ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2552 suspect: Pn S KOG4674 Function unknown Uncharacterized conserved coiled-coil protein
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2552 897356 899257 634
(634 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
At1g79280 [S] KOG4674 Uncharacterized conserved coiled-coil protein 57 9e-08
SPCC162.08c [S] KOG4674 Uncharacterized conserved coiled-coil pr... 56 1e-07
7292701_2 [S] KOG4415 Uncharacterized conserved protein 56 2e-07
YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115 54 7e-07
At1g03830 [R] KOG2037 Guanylate-binding protein 53 2e-06
SPAC1486.04c [S] KOG4674 Uncharacterized conserved coiled-coil p... 52 3e-06
Hs4885399 [D] KOG0964 Structural maintenance of chromosome prote... 51 5e-06
7293243 [D] KOG0964 Structural maintenance of chromosome protein... 51 5e-06
Hs10864047 [TU] KOG0998 Synaptic vesicle protein EHS-1 and relat... 50 8e-06
>At1g79280 [S] KOG4674 Uncharacterized conserved coiled-coil protein
Length = 2111
Score = 57.0 bits (136), Expect = 9e-08
Identities = 59/232 (25%), Positives = 102/232 (43%), Gaps = 39/232 (16%)
Query: 380 QSGPMDIKNYQYILKTLTAERLKQCHVEYEEREKLVLKT--------RLKETKR---LND 428
Q M+ +N++ +LKT E L C +E EKL ++T L+ET R + D
Sbjct: 1321 QKARMESENFENLLKTKQTE-LDLC---MKEMEKLRMETDLHKKRVDELRETYRNIDIAD 1376
Query: 429 FDDLKVNIGQHFKKLKENETKINDSEKRVERTRSELEVLEERL----KSLVERKSEL--- 481
++ LK + Q +KLK + D +K + ++++ +LE+ L K L ER+ L
Sbjct: 1377 YNRLKDEVRQLEEKLKAKDAHAEDCKKVLLEKQNKISLLEKELTNCKKDLSEREKRLDDA 1436
Query: 482 -----VRLLTLEDIAKEIENQKQQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEA 536
+E+E K+ H L+ ++K DE S +N L ++ EA
Sbjct: 1437 QQAQATMQSEFNKQKQELEKNKKIHYTLNMTKRKYEKEKDELSKQNQSLAKQLEEAKEEA 1496
Query: 537 ASEVLKTSALK---AEKEELERQLNGPFTKWELIILQEKEAQLKSSLRKMVQ 585
+ ++ E+EE E+++ IL + QLK +RK +
Sbjct: 1497 GKRTTTDAVVEQSVKEREEKEKRIQ---------ILDKYVHQLKDEVRKKTE 1539
>SPCC162.08c [S] KOG4674 Uncharacterized conserved coiled-coil protein
Length = 1837
Score = 56.2 bits (134), Expect = 1e-07
Identities = 74/276 (26%), Positives = 122/276 (43%), Gaps = 33/276 (11%)
Query: 330 IDSLIKETDPGEFLPSVNPGSSRNND--DLIPFLTNPMELMPLTYGKYELRLQSGPMDIK 387
I L KE + + S+NP + N+ I T + LM +++LR QS +
Sbjct: 1290 IQELEKEVE--KLNASLNPLQTEINELKAEIGAKTASLNLMKEYNSRWKLRFQSV---LN 1344
Query: 388 NYQYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLNDFDDLKVN-IGQHFKKLKEN 446
Y+ + T E K C E E+EK L+T+L+ET + D +VN + + + LK+
Sbjct: 1345 KYERVDPTQLEELKKNC--EALEKEKQELETKLQETAKETDTFKQQVNSLNEEVENLKKE 1402
Query: 447 ETKINDSEKRVERTRSELEVLEERLKSLVERKSELVRLLTL------------EDIAKEI 494
+ N R+ +E E KS + R + L + LT E + KEI
Sbjct: 1403 VEQANTKNTRLAAAWNEK--CENLKKSSLTRFAHLKQELTNKNKELTSKNAENEAMQKEI 1460
Query: 495 ENQKQQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEVLKTSALKAEKEELE 554
E+ K + +L + SS + +E F + L+S ++ E A K E E L+
Sbjct: 1461 ESLKDSNHQLQESASSDAEQITKEQF--EQLKSEKERTEKELADS-------KNELEHLQ 1511
Query: 555 RQLNGPFTKWELIILQEKEAQLKSSLRKMVQQSQFL 590
+ K E+ L+++ +L+S +VQQ Q L
Sbjct: 1512 SEAVDADGKTEISNLEKEIHELRSDKEGLVQQVQNL 1547
Score = 52.0 bits (123), Expect = 3e-06
Identities = 64/288 (22%), Positives = 127/288 (43%), Gaps = 22/288 (7%)
Query: 316 DIKDLISLQQYEN-IIDSLIKETDPGEFLPSVNPGSSRNNDDLIPFLTNPMELMPLTYGK 374
D+++L+S ++E I+D+ + T + S ++ D + N ++ +P++ +
Sbjct: 1198 DLRELVSYLRHEKEIMDNKYELTILDNRGLNQQVKSLQSTVDSLQLELNRLQSLPVSNDQ 1257
Query: 375 YELRLQSGPMDIKNYQYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLNDFDDLKV 434
+ + SG +++ L + + + +E EK V K + ++LK
Sbjct: 1258 TDTPIISGSQEVQLLYESNSVLRKDNDAKLG-KIQELEKEVEKLNASLNPLQTEINELKA 1316
Query: 435 NIGQHFKKL---KENETK-------INDSEKRVERTRSELEVLEERLKSLVERKSELVRL 484
IG L KE ++ + + +RV+ T +LE L++ ++L + K EL
Sbjct: 1317 EIGAKTASLNLMKEYNSRWKLRFQSVLNKYERVDPT--QLEELKKNCEALEKEKQELET- 1373
Query: 485 LTLEDIAKEIENQKQQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQ------KSSEAAS 538
L++ AKE + KQQ L++EV + KK ++ + KN L +++ + KSS
Sbjct: 1374 -KLQETAKETDTFKQQVNSLNEEVENLKKEVEQANTKNTRLAAAWNEKCENLKKSSLTRF 1432
Query: 539 EVLKTSALKAEKEELERQLNGPFTKWELIILQEKEAQLKSSLRKMVQQ 586
LK KE + + E+ L++ QL+ S +Q
Sbjct: 1433 AHLKQELTNKNKELTSKNAENEAMQKEIESLKDSNHQLQESASSDAEQ 1480
>7292701_2 [S] KOG4415 Uncharacterized conserved protein
Length = 1643
Score = 55.8 bits (133), Expect = 2e-07
Identities = 52/202 (25%), Positives = 94/202 (45%), Gaps = 28/202 (13%)
Query: 384 MDIKNYQYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLNDFDDLKVNIGQHFKKL 443
+ +K + L+ E+LK+ ++ +ERE+ + + ++KE +R + K+ + +++
Sbjct: 751 LKLKEREESLRMEREEKLKEEKIKEKEREEKLKEEKIKEKQREEKLKEEKLKEKEREERM 810
Query: 444 KENETKINDSEK-RVERTRSEL-------EVLEERLKSLVERKSELVRLLTLEDIAKEIE 495
KE E + EK R E+ R E E L+E+L+ ++ E L E K E
Sbjct: 811 KEKEREEKAKEKQREEKLREEKIKEKEREEKLKEKLREEKIKEKEKEEKLRKEREEKMRE 870
Query: 496 NQKQQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEVLKTSALKAEKEELER 555
++++ K + V K+ EE K + +++ + E+LK K EKEE ER
Sbjct: 871 KEREEKIKEKERVEKIKEKEREEKLKKE------KEEKLKEKEELLK----KKEKEEKER 920
Query: 556 QLNGPFTKWELIILQEKEAQLK 577
+ L+EKE Q K
Sbjct: 921 EEK----------LKEKERQEK 932
>YDL058w [U] KOG0946 ER-Golgi vesicle-tethering protein p115
Length = 1790
Score = 53.9 bits (128), Expect = 7e-07
Identities = 69/281 (24%), Positives = 124/281 (43%), Gaps = 43/281 (15%)
Query: 325 QYENIIDSLIKETDPGEFLPSVNPGSSRNNDDLIPFLTNPMELMPLTYGKYELRLQSGPM 384
Q EN +SLIK + + S+ + +N D ++ K +++ G +
Sbjct: 975 QAEN--ESLIKAVEESKNESSIQLSNLQNKID------------SMSQEKENFQIERGSI 1020
Query: 385 DIKNYQYILKTLT-AERLKQCHV--------EYEEREKLVLKTRLKETKRLNDF------ 429
+ KN + + KT++ E+ K+ + EYE + L LK +L+ ND
Sbjct: 1021 E-KNIEQLKKTISDLEQTKEEIISKSDSSKDEYESQISL-LKEKLETATTANDENVNKIS 1078
Query: 430 ------DDLKVNIGQHFKKLKENETKINDSEKRVERTRSELEVLEERL----KSLVERKS 479
++L+ + + E ETK+ SEK ++ + E L+E K E K
Sbjct: 1079 ELTKTREELEAELAAYKNLKNELETKLETSEKALKEVKENEEHLKEEKIQLEKEATETKQ 1138
Query: 480 ELVRL-LTLEDIAKEIENQKQQHTKLSDEVSSFKKS-NDEESFKNDDLRSSYQQKSSEAA 537
+L L LE + KE E+ Q K +++++ ++ N+E S ND++ S+ Q+ S
Sbjct: 1139 QLNSLRANLESLEKEHEDLAAQLKKYEEQIANKERQYNEEISQLNDEITSTQQENESIKK 1198
Query: 538 SEVLKTSALKAEKEELERQLNGPFTKWELIILQEKEAQLKS 578
+KA K E Q N ++ + + LQ KE + K+
Sbjct: 1199 KNDELEGEVKAMKSTSEEQSNLKKSEIDALNLQIKELKKKN 1239
>At1g03830 [R] KOG2037 Guanylate-binding protein
Length = 991
Score = 52.8 bits (125), Expect = 2e-06
Identities = 68/276 (24%), Positives = 113/276 (40%), Gaps = 59/276 (21%)
Query: 368 MPLTYGKYELRLQSGPMDIKNYQYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLN 427
M L KYE ++S ++ LK E K+CH+ EE EKLVL+ E++ L
Sbjct: 614 MSLLNEKYE-SVKSASELLETETETLKREKDELDKKCHIHLEELEKLVLRLTNVESEALE 672
Query: 428 D---FDDLKVNIGQHFKKLKENETKINDS--EK--RVERTRSELEVLEE----------- 469
D LK+ + ++NE K+ S E+ ++R +S +E LE+
Sbjct: 673 AKKLVDSLKLEA----EAARDNENKLQTSLVERCIEIDRAKSRIEELEKVCTLNSGEGEA 728
Query: 470 ----------RLKSLVERKSELVRLLTLEDIAKEIENQK-----------------QQHT 502
++++ RK+E LED EI+ K +
Sbjct: 729 SASKKLVDSMKMEAEASRKNENKLQTLLEDKCIEIDRAKSRIEGLERDCLKLKYAESEAA 788
Query: 503 KLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEVLKTSALKAEKEELE-------R 555
+ + VSS K + L+ S Q+K+ E + AL+ +K EL +
Sbjct: 789 TVKELVSSMKMEVESARSNEKKLQLSLQEKTIEIDRAKGQIEALERQKMELSETLETRAK 848
Query: 556 QLNGPFTKWELIILQEKEAQLKSSLRKMVQQSQFLI 591
Q TKW+ II EK ++ +L M ++ F++
Sbjct: 849 QNEEEVTKWQRIINAEKSKNIRENL--MEKEDSFMV 882
>SPAC1486.04c [S] KOG4674 Uncharacterized conserved coiled-coil protein
Length = 1727
Score = 52.0 bits (123), Expect = 3e-06
Identities = 56/235 (23%), Positives = 106/235 (44%), Gaps = 38/235 (16%)
Query: 390 QYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLNDFDDLKVNI------------- 436
QY + E + + E + +++K LN+ + LK +
Sbjct: 571 QYQISNAVKENSNTLSEQIKNLESELNSSKIKNESLLNERNLLKEMLATSRSSILSHNSS 630
Query: 437 -GQHFKKLKENETKINDSEKRVERTRSELEVLEERLKSLVERKSELVRLLTLEDIAKEIE 495
G K+K + + EK E R+E+ ++E SL +R +L L +E I KE+E
Sbjct: 631 AGNIDDKMKSIDESTRELEKNYEVYRNEMTAIQE---SLSKRNQDL--LSEMEAIRKELE 685
Query: 496 NQKQQHTKLSDEVSSFKKSNDEESFKND------------DLRSSYQQKSSEAASEVLKT 543
N K Q +D +++ +ND E+FK + D+ S Q++S+ A E+L
Sbjct: 686 NSKYQQQLSTDRLTN--ANNDVEAFKKEAKELRSINQNLQDIISRQDQRASKFAEELLHV 743
Query: 544 SALKAEKEELERQLNGPFTKWELIILQEKEAQLKSSLRKMVQQSQFLIQYMNRIQ 598
++L E L+ +LN +K E + + + +L S K++ + + L+ ++ +Q
Sbjct: 744 NSL---AERLKGELNA--SKGEKDLRKRTQERLISENDKLLAERERLMSLVSDLQ 793
>Hs4885399 [D] KOG0964 Structural maintenance of chromosome protein 3 (sister
chromatid cohesion complex Cohesin subunit SMC3)
Length = 1217
Score = 51.2 bits (121), Expect = 5e-06
Identities = 46/181 (25%), Positives = 93/181 (50%), Gaps = 15/181 (8%)
Query: 441 KKLKENETKINDSEKRVERTRSELEVLEERLKSLVERKSELVRLLTLEDIAKEIE----N 496
++ +E+ + + ++E + E+ L+ +EERL +L E K EL + + + + +E N
Sbjct: 170 ERKEESISLMKETEGKREKINELLKYIEERLHTLEEEKEELAQYQKWDKMRRALEYTIYN 229
Query: 497 QKQQHTKLS-DEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEV----LKTSALKAEKE 551
Q+ T+ DE+S+ ++++ E+S + D + + K + +V K SA+K EKE
Sbjct: 230 QELNETRAKLDELSAKRETSGEKSRQLRDAQQDARDKMEDIERQVRELKTKISAMKEEKE 289
Query: 552 ELERQLNGPF---TKWELII--LQEKEAQLKSSLRKMVQQSQFLIQYMNRIQGQYNIENE 606
+L + TK EL LQ++ A ++++++ Q L++ + Q + E E
Sbjct: 290 QLSAERQEQIKQRTKLELKAKDLQDELAGNSEQRKRLLKERQKLLEKIEEKQKEL-AETE 348
Query: 607 P 607
P
Sbjct: 349 P 349
>7293243 [D] KOG0964 Structural maintenance of chromosome protein 3 (sister
chromatid cohesion complex Cohesin subunit SMC3)
Length = 1211
Score = 51.2 bits (121), Expect = 5e-06
Identities = 44/211 (20%), Positives = 93/211 (43%), Gaps = 28/211 (13%)
Query: 379 LQSGPMDIKNYQYILKTLTAERLKQCHVEYEEREKLVLKTRLKETKRLNDFDDLKVNIGQ 438
L+ ++K YQ KT + E ++ +K + + +L+ + + I +
Sbjct: 214 LEEEKEELKEYQKWDKTRRTLEYIRYETELKDTKKALDELQLQRKSSSDKKKIYNIEIQK 273
Query: 439 HFKKLKENETKINDSEKRVERTRSELEVLEERLKSLVERKSELVRLLTLEDIAKEIENQK 498
+K+K+ + + +++K+V+ T+ E VL + L+ K++L LT+ D+ E++
Sbjct: 274 AQEKIKDVQKNLKEAKKKVQSTKEERSVLMTEQQQLLREKTKLD--LTIVDLNDEVQGDN 331
Query: 499 QQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEVLKTSALKAEKEELERQLN 558
+ + E+ + K + E + DD++ Y+ A+K ++E+ R+
Sbjct: 332 KSKERADQELKNLKVTIAEREKELDDVKPKYE--------------AMKRKEEDCSRE-- 375
Query: 559 GPFTKWELIILQEKEAQLKSSLRKMVQQSQF 589
LQ KE + K K + SQF
Sbjct: 376 ----------LQLKEQKRKELYAKQGRGSQF 396
>Hs10864047 [TU] KOG0998 Synaptic vesicle protein EHS-1 and related EH domain
proteins
Length = 864
Score = 50.4 bits (119), Expect = 8e-06
Identities = 41/177 (23%), Positives = 80/177 (45%), Gaps = 18/177 (10%)
Query: 426 LNDFDDLKVNIGQHFKKLKENETKINDSEKRVERTRSELEVLEERLKSLVERKSELVRLL 485
+ + DD+ I Q ++ E I + E+ + + SE++ L+ L EL
Sbjct: 383 VKELDDISQEIAQLQREKYSLEQDIREKEEAIRQKTSEVQELQNDLDRETSSLQELEA-- 440
Query: 486 TLEDIAKEIENQKQQHTKLSDEVSSFKKSNDEESFKNDDLRSSYQQKSSEAASEVLKTSA 545
+D ++ QQ KL D +S ++ +E+ L++ Q + S+
Sbjct: 441 QKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQMISSLKTQIQSQESD---------- 490
Query: 546 LKAEKEELERQLNGPFTKWELIILQEKEAQLKSSLRKMVQQSQFLIQYMNRIQGQYN 602
LK+++++L R K EL LQ++E QL+ S++ Q + +I+ + Q + N
Sbjct: 491 LKSQEDDLNR------AKSELNRLQQEETQLEQSIQAGRVQLETIIKSLKSTQDEIN 541
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.314 0.132 0.358
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 34,639,719
Number of Sequences: 60738
Number of extensions: 1502973
Number of successful extensions: 7520
Number of sequences better than 1.0e-05: 9
Number of HSP's better than 0.0 without gapping: 0
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 7445
Number of HSP's gapped (non-prelim): 56
length of query: 634
length of database: 30,389,216
effective HSP length: 113
effective length of query: 521
effective length of database: 23,525,822
effective search space: 12256953262
effective search space used: 12256953262
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)