ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2683 good E KOG2551 Amino acid transport and metabolism Phospholipase/carboxyhydrolase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2683 946417 945692 -242
(242 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase 356 2e-98
SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase 90 3e-18
YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase 81 1e-15
YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase 70 3e-12
SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase 64 1e-10
CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase 59 5e-09
7300631 [E] KOG2551 Phospholipase/carboxyhydrolase 50 4e-06
At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase 49 5e-06
>YHR049w [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 243
Score = 356 bits (913), Expect = 2e-98
Identities = 173/233 (74%), Positives = 201/233 (86%), Gaps = 2/233 (0%)
Query: 8 PKLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKWQET 67
PKLLFL GFLQNGKVFSEKSSGIRKLLKK+NVQCDY+DAPV+LEKKDLPFEMDD KWQ T
Sbjct: 6 PKLLFLHGFLQNGKVFSEKSSGIRKLLKKANVQCDYIDAPVLLEKKDLPFEMDDEKWQAT 65
Query: 68 LNAGVNRAWFYHSDISSELDLSPAIEAVTKYIKDNGPYDGIVGFSQGAALASIITNKITE 127
L+A VNRAWFYHS+IS ELD+S +++V +IK NGPYDGIVGFSQGAAL+SIITNKI+E
Sbjct: 66 LDADVNRAWFYHSEISHELDISEGLKSVVDHIKANGPYDGIVGFSQGAALSSIITNKISE 125
Query: 128 LVPSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRTTKMLFVYGTADMA 187
LVP HPQFKVS++I+ YSFTEPDPE+ G+LRI++KF+DSF D TKM+F+YG +D A
Sbjct: 126 LVPDHPQFKVSVVISGYSFTEPDPEHPGELRITEKFRDSFAVKPDMKTKMIFIYGASDQA 185
Query: 188 VPCERAKYLYNIYTKGQELDDCK--AYEHPGGHMVPNKKDIIRPIVEEINTSL 238
VP R+KYLY+IY K Q + K AYEHPGGHMVPNKKDIIRPIVE+I +SL
Sbjct: 186 VPSVRSKYLYDIYLKAQNGNKEKVLAYEHPGGHMVPNKKDIIRPIVEQITSSL 238
>SPCC1223.08c_1 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 233
Score = 89.7 bits (221), Expect = 3e-18
Identities = 59/224 (26%), Positives = 105/224 (46%), Gaps = 30/224 (13%)
Query: 9 KLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKWQETL 68
K+L L G++Q+G VFS+K ++K L K + + PV+ +++ P + ++ K L
Sbjct: 6 KVLCLHGWIQSGPVFSKKMGSVQKYLSKY-AELHFPTGPVVADEEADPNDEEEKKRLAAL 64
Query: 69 NAGVNRA---WFYHSDISSEL-DLSPAIEAVTKYIKDNGPYDGIVGFSQGAALASIITNK 124
N WF D + ++E + +Y+++ GP+DG++GFSQGA + +++
Sbjct: 65 GGEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQM 124
Query: 125 ITELVP-----SHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRTTKMLF 179
+ P HP FK + + + +P+ F + P TT L
Sbjct: 125 LQPGQPPNPYVQHPPFKFVVFVGGFRAEKPE------------FDHFYNP--KLTTPSLH 170
Query: 180 VYGTADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPNK 223
+ GT+D VP R+K L + ++ HPG H+VP +
Sbjct: 171 IAGTSDTLVPLARSKQLV------ERCENAHVLLHPGQHIVPQQ 208
>YOR280c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 266
Score = 81.3 bits (199), Expect = 1e-15
Identities = 68/236 (28%), Positives = 100/236 (41%), Gaps = 34/236 (14%)
Query: 9 KLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKWQETL 68
K+L L GF+Q+ K+FS K+ G+RK LKK Y AP ++KK L F+ + K ++
Sbjct: 6 KVLMLHGFVQSDKIFSAKTGGLRKNLKKLGYDLYYPCAPHSIDKKAL-FQSESEKGRDAA 64
Query: 69 NAGVNRA-------WFY---HSDISSELDLSPAIEAVTKYIKDNGPYDGIVGFSQGAALA 118
A WF+ S S ++D + Y+ +NGP+DG++GFSQGA L
Sbjct: 65 KEFNTSATSDEVYGWFFRNPESFNSFQID-QKVFNYLRNYVLENGPFDGVIGFSQGAGLG 123
Query: 119 SIIT---NKITELV-PSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRT 174
+ N+I L P K + + + + + E RI Q
Sbjct: 124 GYLVTDFNRILNLTDEQQPALKFFISFSGFKLEDQSYQKEYH-RIIQ------------- 169
Query: 175 TKMLFVYGTADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPNKKDIIRPI 230
L V G D V R LY + + HPG H VPN K + +
Sbjct: 170 VPSLHVRGELDEVVAESRIMALYESWPDNKR----TLLVHPGAHFVPNSKPFVSQV 221
>YMR222c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 223
Score = 69.7 bits (169), Expect = 3e-12
Identities = 57/231 (24%), Positives = 97/231 (41%), Gaps = 26/231 (11%)
Query: 10 LLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKWQ---E 66
+L L G Q+G F+ K+ G R ++K + Y AP D+P + + +
Sbjct: 5 VLMLHGLAQSGDYFASKTKGFRAEMEKLGYKLYYPTAPNEFPPADVPDFLGEVIADAPGD 64
Query: 67 TLNAGVNRAWFYHSDISSELDL-SPAIEAVTKYIKDNGPYDGIVGFSQGAALASIITNKI 125
N GV AW + + + I+ + Y+ +NGP+ GIVGFSQGA +A +
Sbjct: 65 GENTGV-LAWLENDPSTGGYFIPQTTIDYLHNYVLENGPFAGIVGFSQGAGVAGYLATDF 123
Query: 126 TELV----PSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRTTKMLFVY 181
L+ P + + ++ + F Q++Q+ + + + L V
Sbjct: 124 NGLLGLTTEEQPPLEFFMAVSGFRFQ------------PQQYQEQY-DLHPISVPSLHVQ 170
Query: 182 GTADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPNKKDIIRPIVE 232
G D + + LYN T+ D H GGH VPN + +R + +
Sbjct: 171 GELDTITEPAKVQGLYNSCTE----DSRTLLMHSGGHFVPNSRGFVRKVAQ 217
>SPAC22A12.06c [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 429
Score = 64.3 bits (155), Expect = 1e-10
Identities = 65/252 (25%), Positives = 115/252 (44%), Gaps = 46/252 (18%)
Query: 1 MTTATAAPKLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEK-KDLPFEM 59
M +AT + K+L + G+ ++G++FS K +R+ + S V + P+ L+K KD E+
Sbjct: 1 MKSATKS-KILCIHGYAESGELFSVKLRALRERMADS-VDFYFPTGPIELDKAKD---EL 55
Query: 60 DDAKWQ------ETLNAGVNRAWFYHSDISSELDLSP--AIEAVTKYIKDNGPYDGIVGF 111
+ + + + A R W+ ++ + L P A E + YIK++GP+DGI+GF
Sbjct: 56 NGSGFDALSTVFSSSPASHRRGWWRINEYADTKQLEPTKAFEYLASYIKEHGPFDGILGF 115
Query: 112 SQGAALASIITNKITELVPSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKP-- 169
SQG LA+ + +T +P + ++ F++P R + F F+P
Sbjct: 116 SQGTNLAANLAALVT--IPKYQEY----------FSQP------PFRFALFFSGYFRPLL 157
Query: 170 MDD--RTTKM----LFVYGTADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPNK 223
MD TK+ L + G D + E + L + D + HP H +P
Sbjct: 158 MDGAVHATKLDLPTLHLLGKYDTVLSTETSTTLV------RACKDAQVLFHPAAHQIPAP 211
Query: 224 KDIIRPIVEEIN 235
+ P + I+
Sbjct: 212 HAYVEPAADFID 223
>CE05303 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 221
Score = 59.3 bits (142), Expect = 5e-09
Identities = 54/216 (25%), Positives = 105/216 (48%), Gaps = 27/216 (12%)
Query: 9 KLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKWQETL 68
++L L G+ Q + F +K+ RKL+K S + ++++ V D + A W
Sbjct: 8 RILCLHGYRQCDQSFRQKTGSTRKLVK-SLAEFEFVNG-VHSVAVDEHVDSSRAWWFSN- 64
Query: 69 NAGVNRAWFYHSDISSELDLS--PAIEAVTKYIKDNGPYDGIVGFSQGAALASIITNKIT 126
N A + S S+E+ + ++ AV K+I++NGP+DG++GFSQGA++ ++ K
Sbjct: 65 ----NEAMSFSSRESTEVAVGFEESVAAVVKFIEENGPFDGLLGFSQGASMVHLLIAKAQ 120
Query: 127 ELVPSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRTTKMLFVYGTADM 186
P + ++ + G L +S K DS + + + V+G AD
Sbjct: 121 LGEIKLPGIRFAIFFS------------GFLSLSSK-HDSLTLLRIKEFPSMHVFGDADE 167
Query: 187 AVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPN 222
V +++ + +++ +++ + H GGH+VP+
Sbjct: 168 IVARPKSEKMADMF----DVEPLR-IAHDGGHVVPS 198
>7300631 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 279
Score = 49.7 bits (117), Expect = 4e-06
Identities = 51/227 (22%), Positives = 87/227 (37%), Gaps = 43/227 (18%)
Query: 5 TAAPKLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAPVILEKKDLPFEMDDAKW 64
T ++L L G+ QNG+ F K RK K + ++ AP + + + E +
Sbjct: 28 TEKVRVLCLHGYRQNGEAFKNKLGSFRKFANKY-AEFVFITAPHVAKALESAAEPVPEQ- 85
Query: 65 QETLNAGVNRAWFYHSDISS---------ELDLSPAIEAVTKYIKDNGPYDGIVGFSQGA 115
R+W+ + D S ++ V + + GP+ G++GFSQGA
Sbjct: 86 ---------RSWWANKDDGSFKGTNKGGPAFGFQESLRCVEEAWRTQGPFQGLLGFSQGA 136
Query: 116 ALASIITNKITELVPS-HPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDRT 174
+I + + S P+F V A F G L ++++ +
Sbjct: 137 CFVGLICGLAKKKLTSIRPEFAV----LASGFL------SGSLVHMSAYEEAI------S 180
Query: 175 TKMLFVYGTADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVP 221
L +YG D +P E ++ L + + EH GGH P
Sbjct: 181 IPTLHIYGQTDEIIPKEMSESL------AARFKNAEVLEHSGGHYFP 221
>At1g09280_2 [E] KOG2551 Phospholipase/carboxyhydrolase
Length = 229
Score = 49.3 bits (116), Expect = 5e-06
Identities = 60/248 (24%), Positives = 104/248 (41%), Gaps = 43/248 (17%)
Query: 9 KLLFLQGFLQNGKVFSEKSSGIRKLLKKSNVQCDYMDAP----VILEKKDLPFEMDDAKW 64
++L L GF QN F ++ + K LK + ++DAP I + P + + K+
Sbjct: 3 RILCLHGFRQNASSFKGRTGSLAKKLKNI-AELVFIDAPHELQFIYQTATPPSGVCNKKF 61
Query: 65 QETLNAGVNR----AWFYHSDISSELDLSPAIEAVTK---YIK----DNGPYDGIVGFSQ 113
+++ ++ W L E K Y+K + GP+DGI+GFSQ
Sbjct: 62 AWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILGFSQ 121
Query: 114 GAALASIITNKITELVPSHPQFKVSLLIAAYSFTEPDPENEGQLRISQKFQDSFKPMDDR 173
GAA+A+ + K +LV F+ +L + ++ P P E + + S K
Sbjct: 122 GAAMAAAVCGKQEQLV-GEIDFRFCVLCSGFT---PWPLLEMKEKRSIK----------- 166
Query: 174 TTKMLFVYGT---ADMAVPCERAKYLYNIYTKGQELDDCKAYEHPGGHMVPNKKDIIRPI 230
L ++G+ D + + + L ++ G EH GH++P K P
Sbjct: 167 -CPSLHIFGSQPGKDRQIVTQASSDLAGLFEDG----CATIVEHDFGHIIPTKS----PY 217
Query: 231 VEEINTSL 238
++EI L
Sbjct: 218 IDEIKAFL 225
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 15,031,687
Number of Sequences: 60738
Number of extensions: 640005
Number of successful extensions: 1456
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 1444
Number of HSP's gapped (non-prelim): 8
length of query: 242
length of database: 30,389,216
effective HSP length: 103
effective length of query: 139
effective length of database: 24,133,202
effective search space: 3354515078
effective search space used: 3354515078
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)