ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2867 good U KOG2604 Intracellular trafficking, secretion, and vesicular transport Subunit of cis-Golgi transport vesicle tethering complex - Sec34p
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2867 1008738 1010864 709
(709 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YER157w [U] KOG2604 Subunit of cis-Golgi transport vesicle tethe... 486 e-137
SPBC1539.05 [U] KOG2604 Subunit of cis-Golgi transport vesicle t... 175 2e-43
7295805 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethe... 151 3e-36
Hs13899251 [U] KOG2604 Subunit of cis-Golgi transport vesicle te... 121 3e-27
At1g73430 [U] KOG2604 Subunit of cis-Golgi transport vesicle tet... 75 3e-13
CE26779 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethe... 54 8e-07
>YER157w [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 801
Score = 486 bits (1251), Expect = e-137
Identities = 295/796 (37%), Positives = 447/796 (56%), Gaps = 95/796 (11%)
Query: 1 MTRSRRSSIVQDIASK--------------------NLGSHELPVYTDDLLQ---ILKNI 37
M RSR++S+V+DIAS L L V D LQ + +
Sbjct: 1 MARSRKNSLVRDIASHPTIPESQTIVGLLDDSYLFDKLKKLSLAVENSDSLQRTDVSEGC 60
Query: 38 EPHWNPEPLQTANHGIDDKYKEYFDYLEQLDSKNGYYTKLREKSSNIAGKLSLILEKFEV 97
E +A+ +KY Y YL+QL+ K Y + +++ + +L ++KF
Sbjct: 61 SEVNGSEATTSADVKKTNKYLYYTTYLDQLNIKIDEYKVVLDQTRQVNDQLDSSIKKFRK 120
Query: 98 ISADTEAFQLSTDPLAKKLLDSEDTYTQIQNELQYFQNLDPISRKLNHYSSPNIVLRDSF 157
IS DT AF T + +K + I L YF+ LDPI R+LNH +SP IV + SF
Sbjct: 121 ISQDTGAFIEETKTIYEKQSKLSNLTESIPKALHYFEVLDPIMRRLNHATSPAIVKKSSF 180
Query: 158 KQNFVKIDESLKFLEEHSDYLEADSYRIRFKQCLIRACSLVSDYLNNQIKAINTDITLRL 217
IDESL+FL+E+SD +A +YRI+FKQCLIRAC L+S +L N +K N +I +
Sbjct: 181 TTMLATIDESLRFLDENSDLKDAAAYRIKFKQCLIRACELISHFLTNLLKQTNQEILDKT 240
Query: 218 NAGG--TQLAANSQDALLYNKFATVAPTYIQLIGTISEHAR-------YDEIESVLKDCV 268
T L + ++DA LY+KF T+A T+ + I + + +DE+ S+L +C
Sbjct: 241 KNKNSLTGLPSTTRDAFLYSKFYTIADTFKIQVSEIVKRSNEKAYNKYHDELNSILYECF 300
Query: 269 STYFNSRARLLKNSIWLQLNQTIVRSNQ--TVKFIQDNLLYFENLAHREFNLFSQFYPIN 326
+ YF +R RLL IW +++ +V+ VKFIQD +YF+ L E+ LF +F+P
Sbjct: 301 NHYFQTRLRLLTPVIWSHIDEIVVKDKDQGLVKFIQDGKVYFQQLCADEYKLFVEFFP-E 359
Query: 327 EDIETSFNEWCMELFEPLYDCVRTRILRETSISTICDSITLLNKYYQMEETSPEYKLQFQ 386
++ N+W ++L EPLYD +R R+L+ET I T+CDS+TL YY+ EE S EY QF
Sbjct: 360 KECRFKINQWFLQLCEPLYDSIRVRVLKETDICTLCDSVTLFAPYYEFEEGSEEYVKQFT 419
Query: 387 HIKIDMLFKPILKECQSRLIFRSQIYVEENINSYKPKVDSFIIRHRK------------- 433
I+ D LF+PI+++ Q+RLI R QIYV++NI SY+P D F+I +R+
Sbjct: 420 DIQYDKLFEPIVQKVQARLILRVQIYVQQNILSYRPTRDVFMISNRRRKSKTSLQGGNED 479
Query: 434 ---------------------------TPKEAKNKANDLDEISTSFFQLDEFNSAYPPLV 466
+P +A +K+ D +E + QL + YPPL+
Sbjct: 480 ATTSDDNPDPLLESYLSSFKNRSILPISPNDADDKSIDSEESTDKISQLQTY---YPPLL 536
Query: 467 YGIALLSKIYQMVNSTVFDDIAHTIVHDCIMSLKKAFQLV----STNATINXXXXXXXXX 522
+ALLSKIY+M+NS VFDD+AH +VHDCI+SL+ A+ +V + + N
Sbjct: 537 KTLALLSKIYEMINSVVFDDLAHHVVHDCIVSLRNAYDMVIKSSAGKSDFNNLDISLAYL 596
Query: 523 XXXXXXRDQIQNFDIQYISKETYLDFSGL------LK--GINDIRR-RTISVFQMGFDTV 573
RD IQNF+IQY ETYLDFSG+ LK G N +++ ++ S+ + + V
Sbjct: 597 KNLLMLRDSIQNFNIQYTVNETYLDFSGVEGFFKSLKENGRNVLKKTKSSSILTLARELV 656
Query: 574 PKIINNMVDARSELIAELRNTINGITKTAADQITKDYLSISETGDKLLEQNMEVRKSIEI 633
PK++NNMVDAR+ELI+ELRN I T++ + ++ D L I+ D LL +N+++R++I+
Sbjct: 657 PKVVNNMVDARTELISELRNVIKDFTESTSLELIDDTLDINSDED-LLSKNVKLRENIKA 715
Query: 634 NLVSIFDQISNFITNDEVKSHLVDAIQQEVVSRYASYYET---LADSAKIDSQKLSEIMY 690
L I++QI N+I + E+ ++L+DA+Q+ + Y+ YYET LA++ K +++++MY
Sbjct: 716 RLPRIYEQILNYIDDQEIVTNLLDAVQELITQSYSKYYETITELAENGKFAKDQVADVMY 775
Query: 691 IDVFQNFINNETAKLL 706
+DVF +F E A LL
Sbjct: 776 LDVFTDFFAKEVADLL 791
>SPBC1539.05 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 735
Score = 175 bits (444), Expect = 2e-43
Identities = 156/622 (25%), Positives = 280/622 (44%), Gaps = 24/622 (3%)
Query: 64 LEQLDSKNGYYTKLREKSSNIAGKLSLILEKFEVISADTEAFQLSTDPLAKKLLDSEDTY 123
+ +L + ++ +++NI L + EKFE F + L+ + +
Sbjct: 105 INELQTFTNIIDQILGQTNNIESTLLSMKEKFESSEKKLSEFSEMCENLSTDEMRFSEIA 164
Query: 124 TQIQNELQYFQNLDPISRKLNHYSSPNIVLRDSFKQNFVKIDESLKFLEEHSDYLEADSY 183
I+ L F L ++R H P+ + SFK++ +++ + FLEE+ D+ E+ Y
Sbjct: 165 DGIRKGLTIFAPLKELTRVFRH-PPPDFAGKVSFKEHITQLNTCIMFLEENLDFQESPHY 223
Query: 184 RIRFKQCLIRACSLVSDYLNNQIKAINTDITLRLNAGGTQLAANSQDALLYNKFATVAPT 243
++K+ L +A + Y IK TD L+ ++ + +L Y +F+ V
Sbjct: 224 LGQYKKLLSQAMDIFKPYFIRIIKQ-TTDQVLK-DSKKMDVHKQLHSSLFYARFSAVGHN 281
Query: 244 YIQLIGTISEHARYDEIESVLKDCVSTYFNSRARLLKNSIWLQLNQTIVRSNQTVKFIQD 303
I + + + +++ L YF R RLLK + L + + + +IQ
Sbjct: 282 LCPTITELCKLCSKESLDAFLPAFYDVYFQCRTRLLKPVLDYHLKSFFMEKSIS-SYIQK 340
Query: 304 NLLYFENLAHREFNLFSQFYPINEDIETSFNEWCMELFEPLYDCVRTRILRETSISTICD 363
+L + E LF + I +D F + L + ++ R+ IL E +++ +C+
Sbjct: 341 SLALLQLTFFDENKLFREIL-IMDDFR--FMHYWNNLCQSFFENSRSLILHEKNLTELCE 397
Query: 364 SITLLNKYYQMEETSPEYKLQFQHIKIDMLFKPILKECQSRLIFRSQIYVEENINSYKP- 422
+ + + + E + + K+ P++ E Q RL+F Q +E +I Y P
Sbjct: 398 VCSYIQSF---QNAILEGEREDVDKKVVEFLNPLVLELQERLLFVVQTAIETDIQRYSPT 454
Query: 423 KVDSFIIRHRKT---PKEAKNKANDLDEISTSFFQ-LDEFNSAYPPLVYGIALLSKIYQM 478
+ D I K+ E ND +++ Q L YP + + +LSKIY++
Sbjct: 455 EEDLNPIADDKSLLFDLEKLRLNNDEEKLDPDVPQKLAMAQGWYPVVQKSLIILSKIYRL 514
Query: 479 VNSTVFDDIAHTIVHDCIMSLKKAFQLVSTNATINXXXXXXXXXXXXXXXRDQIQNFDIQ 538
VNS VFD+IA +VH CI SL A++ S N + +DQ+ +FDI
Sbjct: 515 VNSQVFDEIALELVHSCIRSLVDAYRYFSRNN--DKQLARLFLIKNFLVLKDQLNSFDIH 572
Query: 539 YISKETYLDFSGLLKGINDIRRRTISVFQMGFDTVPKIINNMVDARSELIAELRNTINGI 598
Y E +D + + + R V Q+ ++T PK I N VD R EL +LR +NG
Sbjct: 573 YACIEAGVDLRKVWDSVREWRSNLRGVLQLVYETFPKFITNAVDTRQELNQQLRVAVNGY 632
Query: 599 TKTAADQITKDYLSISETGDKLLEQNMEVRKSIEINLVSIFDQISNFITNDEVKSHLVDA 658
+TA T + LSI + + E ++K E+ QIS +++ + + A
Sbjct: 633 IETAVIHYT-ECLSIGNL-ESITEFQNRIQKFPELR-----QQISLYLSETWIIELFLQA 685
Query: 659 IQQEVVSRYASYYETLADSAKI 680
I++EVVS++ ++YE++ + I
Sbjct: 686 IREEVVSKFQNFYESIVANEDI 707
>7295805 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 883
Score = 151 bits (382), Expect = 3e-36
Identities = 131/606 (21%), Positives = 266/606 (43%), Gaps = 59/606 (9%)
Query: 59 EYFDYLEQLDSKNGYYTKLREKSSNIAGKLSLILEKFEVISADTEAFQLSTDPLAKKLLD 118
+Y YL+QL+ + + + ++ + +L + ++++ +S T A +++ L ++
Sbjct: 104 DYHKYLQQLEQRKAECSHMLDQIAGAMERLGALCDEYDFVSQKTSALNTASEQLIEEQER 163
Query: 119 SEDTYTQIQNELQYFQNLDPISRKLNHYSSPNI-VLRDSFKQNFVKIDESLKFLEEHSDY 177
++ +IQ L YF ++ ++++L SP + V ++F++ KIDE L ++EE+ +
Sbjct: 164 LQELSHEIQRRLHYFSQVELLNQRLQ---SPTLSVASEAFRECLNKIDECLNYIEENPKF 220
Query: 178 LEADSYRIRFKQCLIRACSLVSDYLNNQIKAINTDITLR-----LNAGGTQLAANSQDAL 232
+A +Y ++++QCL +A LV +Y+ + I T+ TL +A A ++ AL
Sbjct: 221 KDAAAYNVKYRQCLAKASGLVRNYVTSVINQA-TEATLHPKNNMPDASAALKAPDAAFAL 279
Query: 233 LYNKFATVA---PTYIQLIGTISEHARYDEIESVLKDCVSTYFNSRARLLKNSIWLQL-N 288
Y K+ T A QLI + SEH+ + ++ D Y RA ++ ++ L + N
Sbjct: 280 YYGKYQTAAAKVKRVAQLIESRSEHSL--DYAQLMADLQQHYLAQRASVMSPAVNLSIQN 337
Query: 289 QTIVRSNQTVKFIQDNLLYFENLAHREFNLFSQFYPINEDIETSFNEWCMELFEPLYDCV 348
+ + + ++ E LF QF+ T + E + LYD +
Sbjct: 338 VKVAHKGDHCSLTRSACAFLVHVCQDEQRLFYQFFSTGAPHLTVYLEGLCTI---LYDTM 394
Query: 349 RTRILRETSISTICD-----SITLLNKYYQMEETSPEYKLQFQHIKIDMLFKPILKECQS 403
R I+ + T+ + I +L ++ Q + E H +L++ Q
Sbjct: 395 RPFIIHINHLETLAEICSILRIEMLEEHVQQNPVALEAFATIAH--------QLLQDVQE 446
Query: 404 RLIFRSQIYVEENINSYKPK------------VDSFIIR-HRKTPKEAKNKANDLDEIST 450
RL+FR+ +Y++ +I ++ P ++S + P + N + +
Sbjct: 447 RLVFRAHLYLQSDIQNFNPSSGDLAYPEKLEMMESIALSLQEPAPLRRSDSRNSMVSSVS 506
Query: 451 SFFQLDEFNSAY---PPLVYGIALLSKIYQMVNSTVFDDIAHTIVHDCIMSLKKAFQLVS 507
S + + +AY + + LS++Y+ V+ +F ++ + CI S+ A +S
Sbjct: 507 SAVETESVATAYTVKQMIRRTLVCLSRLYRCVDRPIFQGLSQEALKLCIQSVSHAAGKIS 566
Query: 508 TNATINXXXXXXXXXXXXXXXRDQIQNFDIQYISKETYLDFSGLLKGINDIRRRTISVFQ 567
N T R+QI F + + KET LDFS + + ++ +F
Sbjct: 567 ANKT--PIDGELFEIKHLLILREQIAPFRVDFTVKETSLDFSKVKTAAFGLLQKRKQLFS 624
Query: 568 MG---------FDTVPKIINNMVDARSELIAELRNTINGITKTAADQITKDYLSISETGD 618
MG + P+I +++D+R E+ +L++ K A + ++ E
Sbjct: 625 MGSNNALLEFLLEGTPQIKEHLLDSRKEVDRQLKSVCEKYIKDAVQMLVGPLITFLEKAQ 684
Query: 619 KLLEQN 624
LL Q+
Sbjct: 685 SLLAQS 690
>Hs13899251 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 828
Score = 121 bits (304), Expect = 3e-27
Identities = 143/634 (22%), Positives = 256/634 (39%), Gaps = 84/634 (13%)
Query: 56 KYKEYFDYL----EQLDSKNGYYTKLREKSSNIAGKLSLILEKFEVISADTEAFQLSTDP 111
KY++ DYL EQ D+ + ++ L + +++ +S T + +
Sbjct: 126 KYRQMRDYLSGFQEQCDA-------ILNDVNSALQHLESLQKQYLFVSNKTGTLHEACEQ 178
Query: 112 LAKKLLDSEDTYTQIQNELQYFQNLDPISRKLNHYSSPNI-VLRDSFKQNFVKIDESLKF 170
L K+ + D IQ +L YF L+ I+ KLN SP + V D F K+D+ + +
Sbjct: 179 LLKEQSELVDLAENIQQKLSYFNELETINTKLN---SPTLSVNSDGFIPMLAKLDDCITY 235
Query: 171 LEEHSDYLEADSYRIRFKQCLIRACSLVSDYLNNQIKAINTDITLRLNAGGTQLAANSQD 230
+ H ++ + Y ++FKQCL +A L+ Y N ++ + + + R + A++
Sbjct: 236 ISSHPNFKDYPIYLLKFKQCLSKALHLMKTYTVNTLQTLTSQLLKRDPSSVPN--ADNAF 293
Query: 231 ALLYNKFATVAPTYIQLIGTIS-EHARYDEIESVLKDCVSTYFNSRARLLKNSIWLQLNQ 289
L Y KF AP LI I + E + +L D Y + R LL SI + +
Sbjct: 294 TLFYVKFRAAAPKVRTLIEQIELRSEKIPEYQQLLNDIHQCYLDQRELLLGPSIACTVAE 353
Query: 290 TIVRSNQT-VKFIQDNLLYFENLAHREFNLFSQFYPINEDIETSFNEWCMELFEPLYDCV 348
++N+ ++ + ++ E L+++F+ + +E +L LYD
Sbjct: 354 LTSQNNRDHCALVRSGCAFMVHVCQDEHQLYNEFF---TKPTSKLDELLEKLCVSLYDVF 410
Query: 349 RTRILRETSISTICDSITLLNKYYQMEETSPEYKLQFQHIKIDMLFKPILKECQSRLIFR 408
R I+ + T+ + +L K +E+ Q + K +L++ Q RL++R
Sbjct: 411 RPLIIHVIHLETLSELCGIL-KNEVLEDHVQNNAEQLGAFAAGV--KQMLEDVQERLVYR 467
Query: 409 SQIYVEENINSYKPK----------------VDSFIIRHRKTPKEA--------KNKAND 444
+ IY++ +I YKP S +K P EA + ++N
Sbjct: 468 THIYIQTDITGYKPAPGDLAYPDKLVMMEQIAQSLKDEQKKVPSEASFSDVHLEEGESNS 527
Query: 445 LDEI----------STSFFQLDEFNSAYPPLVYGIALLSKIYQMVNSTVFDDIAHTIVHD 494
L + T+ D YP + + LSK+Y+ ++ VF ++ +
Sbjct: 528 LTKSGSTESLNPRPQTTISPADLHGMWYPTVRRTLVCLSKLYRCIDRAVFQGLSQEALSA 587
Query: 495 CIMSLKKAFQLVSTNATINXXXXXXXXXXXXXXXRDQIQNFDIQYISKETYLDFSGLLKG 554
CI SL A + +S N T R+QI F ++ KE LD LK
Sbjct: 588 CIQSLLGASESISKNKT--QIDGQLFLIKHLLILREQIAPFHTEFTIKEISLD----LKK 641
Query: 555 INDIRRRTISVFQMGFDTVPKIIN-NMVDARSELIAELRNTINGITKTAADQITKDYLSI 613
D + ++ TVP+ N +A E + E +I + YL
Sbjct: 642 TRDAAFKILNPM-----TVPRFFRLNSNNALIEFLLE-----------GTPEIREHYLDS 685
Query: 614 SETGDKLLEQNMEVRKSIEINLVSIFDQISNFIT 647
+ D+ L+ E + I+ +Q+ F+T
Sbjct: 686 KKDVDRHLKSACE--QFIQQQTKLFVEQLEEFMT 717
>At1g73430 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 745
Score = 75.5 bits (184), Expect = 3e-13
Identities = 60/222 (27%), Positives = 97/222 (43%), Gaps = 20/222 (9%)
Query: 397 ILKECQSRLIFRSQIYVEENINSYKPKVDSFIIRHRKTPKEAKNKANDLDEISTSFFQLD 456
IL + RL FR++ Y+ + I +Y P + + DL + + D
Sbjct: 372 ILADVNERLTFRARTYIRDEIANYTPSDEDLDYPAKLEGSPNTTSETDLRDDENA----D 427
Query: 457 EFNSAYPPLVYGIALLSKIYQMVNSTVFDDIAHT----IVHDCIMSLKKAFQLVSTNATI 512
F + YPPL ++ LSK+Y+ + VF +A V C +S++KA +L+ +T
Sbjct: 428 VFKTWYPPLEKTLSCLSKLYRCLEQAVFTGLAQARLSEAVEVCSLSIQKASKLIIKRST- 486
Query: 513 NXXXXXXXXXXXXXXXRDQIQNFDIQYISKETYLDFSGLLKGINDIRRRTISVFQMGFDT 572
R+QI FDI++ LDFS LL+ + I R S+F T
Sbjct: 487 -TMDGQLFLIKHLLILREQIAPFDIEFSVTHKELDFSHLLEHLRRILRGQASLFDWSRST 545
Query: 573 ------VPKIINNMVDARSELIAELRNT----INGITKTAAD 604
P+++ + +DA+ EL L+ T I +TK D
Sbjct: 546 SLARTLSPRVLESQIDAKKELEKCLKTTCEEFIMSVTKLVVD 587
>CE26779 [U] KOG2604 Subunit of cis-Golgi transport vesicle tethering
complex - Sec34p
Length = 231
Score = 53.9 bits (128), Expect = 8e-07
Identities = 35/125 (28%), Positives = 62/125 (49%), Gaps = 2/125 (1%)
Query: 74 YTKLREKSSNIAGKLSLILEKFEVISADTEAFQLSTDPLAKKLLDSEDTYTQIQNELQYF 133
+ L+++ N ++ + E +E +S+ T + + D + I+ L YF
Sbjct: 73 FRDLQKQIDNSKATMNELREAYESVSSRTCSLHDACDRALAEQTSLSTGSQLIKTNLYYF 132
Query: 134 QNLDPISRKLNHYSSPNIVLRDSFKQNFVKIDESLKFLEEHSDYLEADSYRIRFKQCLIR 193
+ D I +KL+ + +V SF V IDE L +L H +Y E+++Y +F+QCL R
Sbjct: 133 KQADAIMKKLS--VAKLMVTGHSFAAILVSIDECLTYLRAHPEYKESEAYISKFEQCLSR 190
Query: 194 ACSLV 198
A + V
Sbjct: 191 AMTWV 195
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.318 0.134 0.374
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 38,495,407
Number of Sequences: 60738
Number of extensions: 1618307
Number of successful extensions: 5421
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 5388
Number of HSP's gapped (non-prelim): 9
length of query: 709
length of database: 30,389,216
effective HSP length: 114
effective length of query: 595
effective length of database: 23,465,084
effective search space: 13961724980
effective search space used: 13961724980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)