ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII2897 good A KOG3045 RNA processing and modification Predicted RNA methylase involved in rRNA processing
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII2897 1019168 1020298 377
(377 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YDR083w [A] KOG3045 Predicted RNA methylase involved in rRNA pro... 479 e-135
SPAC56F8.09 [A] KOG3045 Predicted RNA methylase involved in rRNA... 229 4e-60
Hs12758125 [A] KOG3045 Predicted RNA methylase involved in rRNA ... 167 3e-41
At5g40530 [A] KOG3045 Predicted RNA methylase involved in rRNA p... 165 1e-40
CE17217 [A] KOG3045 Predicted RNA methylase involved in rRNA pro... 139 5e-33
7302510 [A] KOG3045 Predicted RNA methylase involved in rRNA pro... 137 2e-32
ECU10g0800 [A] KOG3045 Predicted RNA methylase involved in rRNA ... 85 2e-16
>YDR083w [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 402
Score = 479 bits (1233), Expect = e-135
Identities = 256/379 (67%), Positives = 280/379 (73%), Gaps = 15/379 (3%)
Query: 1 MGLFKVEGWNLDSSKIEFDNAXXXXXXXXXXXXERK---EYQQKLAEQRRNANSKESEPV 57
M LF VEGW++ + + FDN + +QKL E+ ++ E +
Sbjct: 20 MALFNVEGWSIKTKTVAFDNKTNKSSKDKKKNNRKNGKLTREQKLKEETEAELKEQVEDI 79
Query: 58 EQEG-LEEPTPAKQTEKP-----TKKRKIEEVEASQPVLA-----TKKPLTALQQKMMAK 106
EG + + P K EK +KKRK +E V TKK LT LQQKMMAK
Sbjct: 80 PSEGSVAKDIPKKNQEKSDQNETSKKRKHDEEAPLMQVKENIEKPTKKQLTPLQQKMMAK 139
Query: 107 LTGSRFRWINEQLYTISSENALKLIKEQPQLFDEYHDGFRSQVQSWPENPVDVFVEQIRA 166
LTGSRFRWINEQLYTISS+ ALKLIKEQPQLFDEYHDGFRSQVQ+WPENPVDVFV+QIR
Sbjct: 140 LTGSRFRWINEQLYTISSDEALKLIKEQPQLFDEYHDGFRSQVQAWPENPVDVFVDQIRY 199
Query: 167 RANKPVNAPGGLPGLKD-KTIVVADMGCGEAQLALDINNFFXXXXXXXXXXXXXXXXVHS 225
R KPVNAPGGLPGLKD K IV+ADMGCGEAQLAL+INNFF VHS
Sbjct: 200 RCMKPVNAPGGLPGLKDSKEIVIADMGCGEAQLALEINNFFKNYNKKAKKYLKRRHKVHS 259
Query: 226 FDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFINEAYRILAPRGELWIAE 285
FDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFI EAYRILAPRGELWIAE
Sbjct: 260 FDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFIKEAYRILAPRGELWIAE 319
Query: 286 IKSRFGDGKGEEFVNALKLQGFFHKNTDDSNKMFTRFEFFKPPQDIIEERKAKLERRQKF 345
IKSRF DGKG EFV+ALKL GFFHK T D NKMFTRFEFFKPP +IIEER+ KLERRQKF
Sbjct: 320 IKSRFSDGKGNEFVDALKLMGFFHKKTFDENKMFTRFEFFKPPAEIIEERRQKLERRQKF 379
Query: 346 IEVETEKEELQKKREQIPE 364
IEVETEKEEL+KKR +I E
Sbjct: 380 IEVETEKEELEKKRRKIAE 398
>SPAC56F8.09 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 318
Score = 229 bits (585), Expect = 4e-60
Identities = 140/365 (38%), Positives = 185/365 (50%), Gaps = 51/365 (13%)
Query: 15 KIEFDNAXXXXXXXXXXXXERKEYQQKLAEQRR--NANSKESEPVEQEGLEEPTPAKQTE 72
K+++D RKE ++K E++ + K +E V ++ T
Sbjct: 3 KVDWDLGPVSKSASDQTKESRKEKKRKKGERKNVGDKGEKLNEKVLKKAKSVTTNNSLKS 62
Query: 73 KPTKKRKIEEVEASQPVLATKKPLTALQQKMMAKLTGSRFRWINEQLYTISSENALKLIK 132
+ K++ + ++ A LT+LQQKM KL G+ FRWINEQLYT S+ A+++ K
Sbjct: 63 EIKKEKSVPSIKEKNKGDAKHTKLTSLQQKMKDKLDGANFRWINEQLYTTESDKAVQMFK 122
Query: 133 EQPQLFDEYHDGFRSQVQSWPENPVDVFVEQIRARANKPVNAPGGLPGLKDKTIVVADMG 192
E P LFD YH GFR QV+ WPENPVD+F++ ++ R NA K IV+AD+G
Sbjct: 123 ENPDLFDIYHAGFRYQVEGWPENPVDIFIQHLKIRFEHS-NAK------KKNNIVIADLG 175
Query: 193 CGEAQLALDINNFFXXXXXXXXXXXXXXXXVHSFDLKKANERITVADIRNVPLPDESCTI 252
CGEA++A VHSFDL NE + DI NVP+ DE+ I
Sbjct: 176 CGEAKIASTFRK-------------SRSLQVHSFDLVAPNEHVVACDIANVPMADETVDI 222
Query: 253 VVFCLALMGTNFLDFINEAYRILAPRGELWIAEIKSRFGDGKGEEFVNALKLQGFFHKNT 312
VFCL+LMGTN+ F+ EAYRIL G LW+AEIKSRF D GE F L GF K+
Sbjct: 223 AVFCLSLMGTNWQSFLKEAYRILKVGGLLWVAEIKSRFSDKSGEVFAKELPKLGFETKSI 282
Query: 313 DDSNKMFTRFEFFKPPQDIIEERKAKLERRQKFIEVETEKEELQKKREQIPEGKWLLKPC 372
NKMFT FEF K P + K E++P +L C
Sbjct: 283 QLQNKMFTLFEFKKVP--------------------------VHGKCEELPP---ILSAC 313
Query: 373 IYKRR 377
IYKRR
Sbjct: 314 IYKRR 318
>Hs12758125 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 456
Score = 167 bits (422), Expect = 3e-41
Identities = 115/343 (33%), Positives = 160/343 (46%), Gaps = 71/343 (20%)
Query: 35 RKEYQQKLAEQRRNANSKESEPVEQEGLEEPTPAKQTEKPTKKRKIEEVEASQPVLATKK 94
RK+++ + +RR N + V + E K P + E A
Sbjct: 185 RKQWRNRQKNKRRCKNKFQPPQVPDQAPAEAPTEKTEVSPVPRTDSHEARAG-------- 236
Query: 95 PLTALQQKMMAKLTGSRFRWINEQLYTISSENALKLIKEQPQLFDEYHDGFRSQVQSWPE 154
AL+ +M +L G+RFR++NEQLY+ S A +L +E P+ F YH GF+SQV+ WP
Sbjct: 237 ---ALRARMAQRLDGARFRYLNEQLYSGPSSAAQRLFQEDPEAFLLYHRGFQSQVKKWPL 293
Query: 155 NPVDVFVEQIRARANKPVNAPGGLPGLKDKTIVVADMGCGEAQLALDINNFFXXXXXXXX 214
PVD +R R ++VVAD GCG+ +LA I N
Sbjct: 294 QPVDRIARDLRQRP---------------ASLVVADFGCGDCRLASSIRN---------- 328
Query: 215 XXXXXXXXVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFINEAYRI 274
VH FDL + R+TV D+ VPL DES + VFCL+LMGTN DF+ EA R+
Sbjct: 329 -------PVHCFDLASLDPRVTVCDMAQVPLEDESVDVAVFCLSLMGTNIRDFLEEANRV 381
Query: 275 LAPRGELWIAEIKSRFGDGKGEEFVNALKLQGFFHKNTDDSNKMFTRFEFFKPPQDIIEE 334
L P G L +AE+ SRF D + F+ A+ GF + D +N F F+F K ++
Sbjct: 382 LKPGGLLKVAEVSSRFEDVR--TFLRAVTKLGFKIVSKDLTNSHFFLFDFQKTGPPLVGP 439
Query: 335 RKAKLERRQKFIEVETEKEELQKKREQIPEGKWLLKPCIYKRR 377
KA+L Q L+PC+YKRR
Sbjct: 440 -KAQLSGLQ-------------------------LQPCLYKRR 456
>At5g40530 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 285
Score = 165 bits (417), Expect = 1e-40
Identities = 98/297 (32%), Positives = 147/297 (48%), Gaps = 42/297 (14%)
Query: 34 ERKEYQQKLAEQRRNANSKESEPVEQEGLEEPTPAKQTEKPTKKRKIEEVEASQPVLATK 93
E K + + +++RN + EP+E TP Q EK ++ + + +
Sbjct: 5 ENKTSRNRKRKRQRNPKPSKEEPIET------TPKNQNEKKNQRDTKNQQHGGSSAPSKR 58
Query: 94 KPLTALQQKMMAKLTGSRFRWINEQLYTISSENALKLIKEQPQLFDEYHDGFRSQVQSWP 153
+ + +L+G +FR +NE+LYT S + AL KE PQ+FD YH G++ Q+ +WP
Sbjct: 59 PKPSNFLDALRERLSGGQFRMLNEKLYTCSGKEALDYFKEDPQMFDMYHTGYQQQMSNWP 118
Query: 154 ENPVDVFVEQIRARANKPVNAPGGLPGLKDKTIVVADMGCGEAQLALDINNFFXXXXXXX 213
E PV+ + + + ++ ++VVAD GCG+A++A + N
Sbjct: 119 ELPVNSIINWLLSNSS---------------SLVVADFGCGDARIAKSVKN--------- 154
Query: 214 XXXXXXXXXVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDFINEAYR 273
V SFDL N + D+ N L S + VFCL+LMGTN+ +I EA+R
Sbjct: 155 --------KVFSFDLVSKNPSVIACDMSNTSLESSSVDVAVFCLSLMGTNYSSYIKEAHR 206
Query: 274 ILAPRGELWIAEIKSRFGDGKG----EEFVNALKLQGFFHKNTDDSNKMFTRFEFFK 326
+L P G L IAE+KSRF G ++FV A+ GF D SNKMF F F K
Sbjct: 207 VLRPSGMLLIAEVKSRFDPNNGGADPKDFVKAVCDLGFTSVLKDFSNKMFILFHFKK 263
>CE17217 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 343
Score = 139 bits (351), Expect = 5e-33
Identities = 102/350 (29%), Positives = 154/350 (43%), Gaps = 73/350 (20%)
Query: 40 QKLAEQRRNANSKESEPVEQEGLEEPTPAKQTEKPTKKRKIEEVEASQPVLATKKPLTAL 99
+KLA ++ A+ K P E + EP + K+++ + + +P +A K T
Sbjct: 55 RKLAAKKAAADKKSENPEEPPLILEPKSSSDENTKKKRKRGPKKKKFKPEVAGKAAETEN 114
Query: 100 QQKMMA------------KLTGSRFRWINEQLYTISSENALKLIKEQPQLFDEYHDGFRS 147
A +L RFR++NE+LYT + A KE P FD YH GF
Sbjct: 115 DDVAAAPEEADPIAEAKKRLDAGRFRFLNEKLYTCTGSEAFDFFKEDPTAFDLYHKGFAD 174
Query: 148 QVQSWPENPVDVFVEQIRARANKPVNAPGGLPGLKDKTIVVADMGCGEAQLALDINNFFX 207
QV+ WP +P+ + ++++ ++ V D+GCGEA++A +
Sbjct: 175 QVKKWPNHPLREIIRWLQSKPDQQS---------------VFDLGCGEAKIAEAVGE--- 216
Query: 208 XXXXXXXXXXXXXXXVHSFDLKKANERITVADIRNVPLPDESCTIVVFCLALMGTNFLDF 267
+ SFDL N+R+ D+ +P D S IV++CL+LMGTN DF
Sbjct: 217 ------------KHKIRSFDLVAVNDRVESCDMSKLPAEDSSADIVIYCLSLMGTNLYDF 264
Query: 268 INEAYRILAPRGELWIAEIKSRFGDGKGEEFVNALKLQGFFHKNTDDSNKMFTRFEFFKP 327
I EA R+L G L IAE+ SRF K +F A+ GF + + F EF K
Sbjct: 265 IREARRVLKIGGILKIAEVTSRFVSIK--QFCEAITKMGFEQSHRRELTDYFMMMEFKK- 321
Query: 328 PQDIIEERKAKLERRQKFIEVETEKEELQKKREQIPEGKWLLKPCIYKRR 377
E++++KR P G LKPC+YK+R
Sbjct: 322 ------------------------VEKVEQKR---PYG-LKLKPCLYKKR 343
>7302510 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 358
Score = 137 bits (346), Expect = 2e-32
Identities = 98/321 (30%), Positives = 146/321 (44%), Gaps = 74/321 (23%)
Query: 65 PTPAKQTEKPTKKRKIE----EVEASQPVLATKKPLTALQQKMMAKLTGSRFRWINEQLY 120
PT A + K K K E +EA + +T +L K+ ++L G RFR+INEQLY
Sbjct: 104 PTQATKGGKKELKLKPELAQAALEAMEATSSTTPAANSLASKLQSELLGGRFRYINEQLY 163
Query: 121 TISSENALKLIKEQPQLFDEYHDGFRSQVQSWPENPVDVFVEQIRARANKPVNAPGGLPG 180
+ +S A L ++ F+ YH G+R QV+ WP NP++ ++ I+ +P
Sbjct: 164 STTSRKAEALFRKDSSAFEAYHAGYRQQVEKWPINPLNRIIKTIKK-----------IP- 211
Query: 181 LKDKTIVVADMGCGEAQLALDINNFFXXXXXXXXXXXXXXXXVHSFDLKKANERITVADI 240
KT ++ D GCGE +LA + N V+S DL A I +I
Sbjct: 212 ---KTAIIGDFGCGEGKLAQSVPN-----------------KVYSMDLVAARSDIIACNI 251
Query: 241 RNVPLPDESCTIVVFCLALMGTNFLDFINEAYRILAPRGELWIAEIKSRFGDGKGEEFVN 300
+ PL + + V+CL+LMGT+ +F EA R+L G ++IAEI+SRF D + EFV
Sbjct: 252 TDTPLQARTLDVAVYCLSLMGTDLNEFFLEANRVLKLHGTVYIAEIQSRFQDVR--EFVR 309
Query: 301 ALKLQGFFHKNTDDSNKMFTRFEFFKPPQDIIEERKAKLERRQKFIEVETEKEELQKKRE 360
L GF D + F F+F KK
Sbjct: 310 CLNACGFDLNKKDVAVNYFYFFQF--------------------------------KKMR 337
Query: 361 QIPEG----KWLLKPCIYKRR 377
+P+ + LKPC+Y++R
Sbjct: 338 HVPKNTKMKAFSLKPCLYRKR 358
>ECU10g0800 [A] KOG3045 Predicted RNA methylase involved in rRNA processing
Length = 210
Score = 85.1 bits (209), Expect = 2e-16
Identities = 68/226 (30%), Positives = 104/226 (45%), Gaps = 46/226 (20%)
Query: 99 LQQKMMAKLTGSRFRWINEQLYTISSENALKLIKEQPQLFDEYHDGFRSQVQSWPENPVD 158
L++K+ +L G +FR +N+++Y + L K +L YH+ + QV WP NP+D
Sbjct: 3 LEEKITKRLEGGKFRMLNDKMY-----HGKGLRKGDLRL---YHELYNLQVMKWPVNPLD 54
Query: 159 VFVEQIRARANKPVNAPGGLPGLKDKTIVVADMGCGEAQLALDINNFFXXXXXXXXXXXX 218
V +E+I+ R V+AD+GCGEA++A + N
Sbjct: 55 VIIEKIKRREGNG---------------VIADIGCGEARIAREFEN-------------- 85
Query: 219 XXXXVHSFDLKKANERITVADI-RNVPLPDESCTIVVFCLALMGTNFLDFINEAYRILAP 277
V S DL + + D+ R +PL D S + V CL++M + E RIL
Sbjct: 86 ----VISLDLHPVGKDVIPCDMRRRIPLDDGSVDVAVCCLSMMVEDIAVPTKEINRILRN 141
Query: 278 RGELWIAEIKSRFGD-GKGEEFVNALKLQGFFHKNTDDSNKMFTRF 322
G +IAE++SR G E +L GF ++ D SN F F
Sbjct: 142 GGHWYIAEVRSRIPSVGLLERKFESL---GFDVEHVDVSNAQFVLF 184
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.135 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,862,229
Number of Sequences: 60738
Number of extensions: 941635
Number of successful extensions: 6264
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 6236
Number of HSP's gapped (non-prelim): 11
length of query: 377
length of database: 30,389,216
effective HSP length: 108
effective length of query: 269
effective length of database: 23,829,512
effective search space: 6410138728
effective search space used: 6410138728
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)