ORF      STATUS       Function Best COG  Functional category                                          Pathways and functional systems
r_klactIII3074  suspect: LH   T     KOG2830  Signal transduction mechanisms                                 Protein phosphatase 2A-associated protein

BLASTP 2.2.3 [May-13-2002]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= r_klactIII3074 1086129 1085047 -361 
         (361 letters)

Database: KOG eukaryal database 04/03 
           60,738 sequences; 30,389,216 total letters

Searching..................................................done

Color Key for Alignment Scores:   


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

YMR028w [T] KOG2830 Protein phosphatase 2A-associated protein         239   6e-63
SPCC63.05 [T] KOG2830 Protein phosphatase 2A-associated protein        91   2e-18

>YMR028w [T] KOG2830 Protein phosphatase 2A-associated protein
          Length = 366

 Score =  239 bits (609), Expect = 6e-63
 Identities = 142/355 (40%), Positives = 198/355 (55%), Gaps = 15/355 (4%)

Query: 16  QIKESASRQDSKEYQEILTKCLAHGLNFKTFIQTKSHLFSTNETLDDVSTKSLRFLSLDY 75
           +++ ++ RQDS EYQ +L   +   LN KT I  +  LFSTNET+DDVST S++FL++DY
Sbjct: 18  KLEHTSLRQDSPEYQGLLLSTIKKLLNLKTAIFDRLALFSTNETIDDVSTASIKFLAVDY 77

Query: 76  YLAQMMARKQVVTGSPAQRNIVRLKFLQKTXXXXXXXXXXXXDYCILPESLSKRVDKFES 135
           YL  +++R+Q      AQR  ++L +L+K+            DY +L   + +++  F+ 
Sbjct: 78  YLGLLISRRQSNDSDVAQRQSMKLIYLKKSVESFINFLTLLQDYKLLDPLVGEKLGNFKD 137

Query: 136 LWEPKLEELYSSAKD--ELKDATLKRQEKIELYRTGRE-------MEMQIKXXXXXXXXX 186
            + P+L ELY+  K+  +L  A LKR+EKIEL++  +E       +E+++K         
Sbjct: 138 RYNPQLSELYAQPKNNKDLSGAQLKRKEKIELFQRNKEISTKLHCLELELKNNDEDHDHD 197

Query: 187 XXXXXXXXXXXXXXXXXXXXASESWXXXXXXXXXXXXXXXFTNSVSEDNSEERVPDTDTS 246
                                 E                   NSV E  S       +++
Sbjct: 198 ELLRELYLMRLHHFSLDTINNIEQ-----NLFECEMLSNFLKNSVHEVKSSGTQIRKESN 252

Query: 247 EKDPTGYTEKLESLNKPLISKSGKVLRNFTLLDKKDQLKSKVFGYGQYGPTMTVEEFLAK 306
           + D TG+T+KLE++NKPLI K G+VLRNFTL+DK+ QL+ KV GYGQYGPTM+VEEFL K
Sbjct: 253 DDDSTGFTDKLENINKPLIDKKGQVLRNFTLVDKRQQLQQKVRGYGQYGPTMSVEEFLDK 312

Query: 307 EFEENRVLQGGAXXXXXXXXXXXXXSNDLATYKARAWDDFTESNPKGSGNTMNRG 361
           EFEE RVLQGG                D  TYKAR WD+F ES+ KGSGNTMNRG
Sbjct: 313 EFEEGRVLQGGEEPEQAPDEENMDW-QDRETYKAREWDEFKESHAKGSGNTMNRG 366


>SPCC63.05 [T] KOG2830 Protein phosphatase 2A-associated protein
          Length = 323

 Score = 91.3 bits (225), Expect = 2e-18
 Identities = 90/363 (24%), Positives = 147/363 (39%), Gaps = 47/363 (12%)

Query: 4   SQEYDKFLKEFAQIKESASRQDSKEYQEILT---KCLAHGLNFKTFIQTKSHLFSTNETL 60
           S+   +  +E  ++K+S+S  D K   EI+    KCL      K  +Q +  +FS+NE +
Sbjct: 3   SKSLRELWEETEKLKDSSST-DEKTRSEIVEGYEKCL------KLVLQLR--IFSSNEEV 53

Query: 61  DDVSTKSLRFLSLDYYLAQMMARKQVVTGSPAQRNIVRLKFLQKTXXXXXXXXXXXXDYC 120
           D++ T  LR+L +DY LA+ +  +Q   G        RLK +Q              DY 
Sbjct: 54  DEIKTSELRYLMIDYELAKCV--EQWTKGD-------RLKAVQYAKTHYETFLSICDDYG 104

Query: 121 ILPESLSKRVDKFESLWEPKLEELYSSAKDELKDATLKRQEKIELYRTGREMEMQIKXXX 180
           + P    K                    K E    TLK    I  YR  + +E ++K   
Sbjct: 105 LKPMQDEK-------------------PKTEADTRTLK----IARYRMRQNLEKELKALS 141

Query: 181 XXXXXXXXXXXXXXXXXXXXXXXXXXASESWXXXXXXXXXXXXXXXFTNSVSEDNSEERV 240
                                      S                    +  S +  EE +
Sbjct: 142 KDSETNEEQERKFWLTKLQIAVEDTLDSLPHIEMEIDLLKRAQAELMKSEDSPEKDEETL 201

Query: 241 PDTDTSEKDPTGYTEKLESLNKPLISKSGKVLRNFTLLDKKDQLKSKVFGYGQYGPTMTV 300
              +  +K+ + +   L + +K ++ K+ + L+ FT++  +++ +  VFG+G   PTMTV
Sbjct: 202 RREERKQKEGSSWRLDLNTRDK-ILDKNNRPLQPFTIVSDRNETRKNVFGFGYNLPTMTV 260

Query: 301 EEFLAKEFEENRVL-QGGAXXXXXXXXXXXXXSNDLATYKARAWDDFTESNPKGSGNTM- 358
           +E+L +E +   ++ Q                  D  T K R WD+F E+NP+GSGNTM 
Sbjct: 261 DEYLDEEMKRGNIISQKDNPPKSDSDDEDDYEKLDAKTMKDRYWDEFKEANPRGSGNTMV 320

Query: 359 NRG 361
           NRG
Sbjct: 321 NRG 323


  Database: KOG eukaryal database 04/03
    Posted date:  Apr 14, 2003  1:07 PM
  Number of letters in database: 30,389,216
  Number of sequences in database:  60,738
  
Lambda     K      H
   0.311    0.128    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,900,355
Number of Sequences: 60738
Number of extensions: 588150
Number of successful extensions: 1611
Number of sequences better than 1.0e-05: 2
Number of HSP's better than  0.0 without gapping: 1
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 1605
Number of HSP's gapped (non-prelim): 4
length of query: 361
length of database: 30,389,216
effective HSP length: 108
effective length of query: 253
effective length of database: 23,829,512
effective search space: 6028866536
effective search space used: 6028866536
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)