ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3082 good O KOG0314 Posttranslational modification, protein turnover, chaperones Predicted E3 ubiquitin ligase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3082 1087043 1088314 424
(424 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YKL059c [O] KOG0314 Predicted E3 ubiquitin ligase 417 e-116
SPBP8B7.15c [O] KOG0314 Predicted E3 ubiquitin ligase 242 9e-64
Hs22068920 [O] KOG0314 Predicted E3 ubiquitin ligase 103 6e-22
7291741_1 [O] KOG0314 Predicted E3 ubiquitin ligase 98 3e-20
At5g47430 [O] KOG0314 Predicted E3 ubiquitin ligase 91 4e-18
At4g17410 [O] KOG0314 Predicted E3 ubiquitin ligase 89 1e-17
CE28930_1 [O] KOG0314 Predicted E3 ubiquitin ligase 69 1e-11
ECU09g0340 [O] KOG0314 Predicted E3 ubiquitin ligase 67 7e-11
>YKL059c [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 441
Score = 417 bits (1071), Expect = e-116
Identities = 212/365 (58%), Positives = 261/365 (71%), Gaps = 15/365 (4%)
Query: 1 MSSTIYYKFRSQKDTFRVGFDGTGITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEYE 60
MSSTI+Y+F+SQ++T R+ FDGTG+TVFDLKREII +NKLGDGTDFQLKIYNPDT EEY+
Sbjct: 1 MSSTIFYRFKSQRNTSRILFDGTGLTVFDLKREIIQENKLGDGTDFQLKIYNPDTEEEYD 60
Query: 61 DDTTVIGRSSMVIAKRCPSVK-LSVN-------GKSVIGNATRYVMGKPRVMSKRAMTPS 112
DD VI RS+ VI KR P++K SV+ G + +GNATRYV G+PRV+ KR T +
Sbjct: 61 DDAFVIPRSTSVIVKRSPAIKSFSVHSRLKGNVGAAALGNATRYVTGRPRVLQKRQHTAT 120
Query: 113 VTSSGNAKVEGQTEEERIANMFANXXXXXXXXXXXXXXXXPIFQQRNVGSAPGQDQE-PP 171
T A V G TEEERIA+MFA P+F + Q+ E PP
Sbjct: 121 TT----ANVSGTTEEERIASMFATQENQWEQTQEEMSAATPVFFKSQTNKNSAQENEGPP 176
Query: 172 PPGYMCYRCGGRGHWIKNCPTNNNPNFEGKRIKRTTGIPKKFLKSIEIDPSSMTPEEMAE 231
PPGYMCYRCGGR HWIKNCPTN++PNFEGKRI+RTTGIPKKFLKSIEIDP +MTPEEMA+
Sbjct: 177 PPGYMCYRCGGRDHWIKNCPTNSDPNFEGKRIRRTTGIPKKFLKSIEIDPETMTPEEMAQ 236
Query: 232 KKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPDLPDELKCCLTG 291
+KIM+TDEGKFVV + D+ SW ++ KG F DLPD+LKC LTG
Sbjct: 237 RKIMITDEGKFVVQVEDKQSWEDYQRKRENRQIDGDET--IWRKGHFKDLPDDLKCPLTG 294
Query: 292 GLLRDPVKCTKCCGKVVSRLAMEDALLESDFVCPLCQTSDILLDSLEPVQEIQRKVQEFI 351
GLLR PVK +KCC S+ A+E+AL+ESDFVCP C+T DILLDSL P Q+ +++V+ F+
Sbjct: 295 GLLRQPVKTSKCCNIDFSKEALENALVESDFVCPNCETRDILLDSLVPDQDKEKEVETFL 354
Query: 352 KQNKK 356
K+ ++
Sbjct: 355 KKQEE 359
>SPBP8B7.15c [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 482
Score = 242 bits (617), Expect = 9e-64
Identities = 146/368 (39%), Positives = 198/368 (53%), Gaps = 38/368 (10%)
Query: 1 MSSTIYYKFRSQKDTFRVGFDGT-GITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEY 59
MS IYYKF+SQKD R+ FDGT G++VFD+KREII KLG+G DF L +YN ++ EEY
Sbjct: 1 MSGVIYYKFKSQKDPSRITFDGTIGMSVFDVKREIIMQKKLGNGLDFDLLLYNANSNEEY 60
Query: 60 EDDTTVIGRSSMVIAKRCPSVKLSVNGKSVIGNATRYVMGKPR--------------VMS 105
+DDT +I RS+ VI +R P+ K +GK G A RYV G P+ ++
Sbjct: 61 DDDTFIIPRSTSVIVRRVPAQK---SGK---GTAARYVSGAPKTTGARSDSVKRPVPMLQ 114
Query: 106 KRAMTPSVTSSGNAKVEGQTEEERIANMFANXXXXXXXXXXXXXXXXPIFQQRNVGSAPG 165
K+A S S+ N K +E+ I MF PI++ A
Sbjct: 115 KKAPITSGESNIN-KSPSSSEDAAIQQMFQVSSDQWRETQDKMASATPIYKPNQRRIAAS 173
Query: 166 QDQEPPPPGYMCYRCGGRGHWIKNCPTNNNPNFEGK-RIKRTTGIPKKFLKSIEIDPSSM 224
+PPPPGY+CYRCG +GHWI+ CPTN +PN++GK R+KRTTGIP+ FLK++E
Sbjct: 174 VPDKPPPPGYICYRCGQKGHWIQACPTNADPNYDGKPRVKRTTGIPRSFLKNVE------ 227
Query: 225 TPEEMAEKKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPDLPDE 284
P E IM+ EG +VV D SW K Q P++
Sbjct: 228 RPAEGDAANIMINAEGDYVVVQPDVASWETYQSRKAALTAND------VYKMQPPNI--S 279
Query: 285 LKCCLTGGLLRDPVKCTKCCGKVVSRLAMEDALLESDFVCPLCQTSDILLDSLEPVQEIQ 344
L C L L R+ + T CC K+ ++ ALL+SDF CP C D+LLD+L P + Q
Sbjct: 280 LACTLCKKLARNACR-TPCCDKLFCEECIQTALLDSDFECPNCHRKDVLLDTLNPDYQKQ 338
Query: 345 RKVQEFIK 352
R+++ +K
Sbjct: 339 REIEAVVK 346
>Hs22068920 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 1663
Score = 103 bits (256), Expect = 6e-22
Identities = 62/183 (33%), Positives = 91/183 (48%), Gaps = 16/183 (8%)
Query: 170 PPPPGYMCYRCGGRGHWIKNCPTNNNPNFE-GKRIKRTTGIPKKFLKSIEIDPSSMTPEE 228
PPPP Y C+RCG GH+IKNCPTN + NFE G RIK++TGIP+ F+ ++ DP+
Sbjct: 59 PPPPSYTCFRCGKPGHYIKNCPTNGDKNFESGPRIKKSTGIPRSFMMEVK-DPNM----- 112
Query: 229 MAEKKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPDLPDELKCC 288
K M+T+ GK+ + D ++ + +PDEL C
Sbjct: 113 ---KGAMLTNTGKYAIPTIDAEAYAIGKKEKPPFLPEEPSSS----SEEDDPIPDELLCL 165
Query: 289 LTGGLLRDPVKCTKCCGKVVSRLAMEDALLESD-FVCPLCQTSDILLDSLEPVQEIQRKV 347
+ ++ D V CCG + ALLESD CP C +D+ D+L + +++ V
Sbjct: 166 ICKDIMTDAV-VIPCCGNSYCDECIRTALLESDEHTCPTCHQNDVSPDALIANKFLRQAV 224
Query: 348 QEF 350
F
Sbjct: 225 NNF 227
>7291741_1 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 286
Score = 97.8 bits (242), Expect = 3e-20
Identities = 92/352 (26%), Positives = 142/352 (40%), Gaps = 78/352 (22%)
Query: 4 TIYYKFRSQKDTFRVGFDGTGITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEYEDDT 63
+++YKF+S + + FDG I+V DLKREI+ +LG DF L+I N + EEY+DD
Sbjct: 2 SVHYKFKSTLNFDTITFDGLHISVGDLKREIVQQKRLGKIIDFDLQITNAQSKEEYKDDG 61
Query: 64 TVIGRSSMVIAKRCPSVKLSVNGKSVIGNATRYVMGKPRVMSKRAMTPSVTSSGN---AK 120
+I +++ +I R P I + T+ P + + P+ + N +K
Sbjct: 62 FLIPKNTTLIISRIP-----------IAHPTKKGWEPPAAENAFSAAPAKQDNFNMDLSK 110
Query: 121 VEGQTEEERIANMFANXXXXXXXXXXXXXXXXPIFQQRNVGSAPGQDQ-EPPPPGYMCYR 179
++G TEE++I M + + GQ Q P Y C +
Sbjct: 111 MQG-TEEDKIQAMMMQSTVD--------------YDPKTYHRIKGQSQVGEVPASYRCNK 155
Query: 180 CGGRGHWIKNCPTNNNPNFEGKRIKRTTGIPKKFLKSIEIDPSSMTPEEMAEKKIMVTDE 239
C GHWIKNCP + + +KR TGIP+ F D E A+
Sbjct: 156 CKKSGHWIKNCPFVGGK--DQQEVKRNTGIPRSFR-----DKPDAAENESAD-------- 200
Query: 240 GKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPDLPDELKCCLTGGLLRDPVK 299
FV+ Q ++P++L C + + D V
Sbjct: 201 --FVLPAV-----------------------------QNQEIPEDLICGICRDIFVDAV- 228
Query: 300 CTKCCGKVVSRLAMEDALLES-DFVCPLCQTSDILLDSLEPVQEIQRKVQEF 350
CCG + +LLES D CP C+ + SL P + ++ V F
Sbjct: 229 MIPCCGSSFCDDCVRTSLLESEDSECPDCKEKNCSPGSLIPNRFLRNSVNAF 280
>At5g47430 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 879
Score = 90.5 bits (223), Expect = 4e-18
Identities = 56/192 (29%), Positives = 91/192 (47%), Gaps = 27/192 (14%)
Query: 161 GSAPGQDQEPPPPGYMCYRCGGRGHWIKNCPTNNNPNFEGKRIKRTTGIPKKFLKSIEID 220
G G +++ PPPGY+C+RC GH+I++CPTN +PN++ KR+K TGIPK L +
Sbjct: 194 GRGFGMERKTPPPGYVCHRCNIPGHFIQHCPTNGDPNYDVKRVKPPTGIPKSMLMATPDG 253
Query: 221 PSSMTPEEMAEKKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPD 280
S+ +A ++ +E F M S +
Sbjct: 254 SYSLPSGAVA---VLKPNEDAFEKEMEGLPSTTRSVG----------------------E 288
Query: 281 LPDELKCCLTGGLLRDPVKCTKCCGKVVSRLAMEDALLESDFVCPLCQTSDILLDSLEPV 340
LP ELKC L +++D +KCC K + D ++ S +C +C SD+L D L P
Sbjct: 289 LPPELKCPLCKEVMKDAALTSKCCYKSFCDKCIRDHII-SKSMC-VCGRSDVLADDLLPN 346
Query: 341 QEIQRKVQEFIK 352
+ ++ + ++
Sbjct: 347 KTLRDTINRILE 358
Score = 55.8 bits (133), Expect = 1e-07
Identities = 28/74 (37%), Positives = 42/74 (55%)
Query: 5 IYYKFRSQKDTFRVGFDGTGITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEYEDDTT 64
IYYKF+S +D + DG I+V LK +I LG G D + + N T EEY D+
Sbjct: 3 IYYKFKSARDYDTIAMDGPFISVGILKDKIFETKHLGTGKDLDIVVSNAQTNEEYLDEAM 62
Query: 65 VIGRSSMVIAKRCP 78
+I +++ V+ +R P
Sbjct: 63 LIPKNTSVLIRRVP 76
>At4g17410 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 744
Score = 89.0 bits (219), Expect = 1e-17
Identities = 56/192 (29%), Positives = 87/192 (45%), Gaps = 27/192 (14%)
Query: 161 GSAPGQDQEPPPPGYMCYRCGGRGHWIKNCPTNNNPNFEGKRIKRTTGIPKKFLKSIEID 220
G G ++ PPPGY+C+RC GH+I++C TN NPNF+ KR+K TGIPK L +
Sbjct: 105 GRGFGMERTTPPPGYVCHRCNVSGHFIQHCSTNGNPNFDVKRVKPPTGIPKSMLMATPNG 164
Query: 221 PSSMTPEEMAEKKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPD 280
S+ +A ++ +E F M TS G+F
Sbjct: 165 SYSLPSGAVA---VLKPNEDAFEKEMEGLTSTTRSV-------------------GEF-- 200
Query: 281 LPDELKCCLTGGLLRDPVKCTKCCGKVVSRLAMEDALLESDFVCPLCQTSDILLDSLEPV 340
P ELKC L ++RD +KCC K + D ++ +C + +L D L P
Sbjct: 201 -PPELKCPLCKEVMRDAALASKCCLKSYCDKCIRDHIIAKSMC--VCGATHVLADDLLPN 257
Query: 341 QEIQRKVQEFIK 352
+ ++ + ++
Sbjct: 258 KTLRDTINRILE 269
>CE28930_1 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 377
Score = 68.9 bits (167), Expect = 1e-11
Identities = 58/189 (30%), Positives = 82/189 (42%), Gaps = 18/189 (9%)
Query: 3 STIYYKFRSQKDTFRVGFDGTGITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEYEDD 62
S+I+YKFR++ D + FDG I L REI L F+L++ N T + Y DD
Sbjct: 2 SSIHYKFRAELDYKTLQFDGLHIRGEQLVREICAKENLKLEL-FELQLQNAHTKKTYSDD 60
Query: 63 TTVIGRSSMVIAKRCPSVKLSVNGKSVIGNATRYVMGKPRVMSKRAMTPSVTSSGNAKVE 122
+I R+S +I +R P + K G + V S + ++S A+ E
Sbjct: 61 E-LIPRNSSIIVQRFPRKDAAKVQKVQAGVNSGMVNQLDATSSFLDPSSHISS---AEFE 116
Query: 123 GQTEEERIANMFANXXXXXXXXXXXXXXXXPIFQQRNVGSAPGQDQEPPPPGYMCYRCGG 182
E ER+ ++ F++R G G PPPP Y C RC
Sbjct: 117 NMDEAERLNHIRDQSTRAYDQSN---------FRRRQPGIMTG----PPPPTYTCNRCSQ 163
Query: 183 RGHWIKNCP 191
GHW KNCP
Sbjct: 164 PGHWYKNCP 172
>ECU09g0340 [O] KOG0314 Predicted E3 ubiquitin ligase
Length = 260
Score = 66.6 bits (161), Expect = 7e-11
Identities = 49/186 (26%), Positives = 84/186 (44%), Gaps = 36/186 (19%)
Query: 172 PPGYMCYRCGGRGHWIKNCPTNNNPNFEGKRIKRTTGIPKKFLKSI--EIDPSSMTPEEM 229
P Y+C+RCG +GH+I+NC TN + F+ RI++ +GIPK FL + E PS+
Sbjct: 109 PESYVCFRCGQKGHYIQNCHTNQDKAFDILRIRKPSGIPKDFLVPVYGENVPSNAA---- 164
Query: 230 AEKKIMVTDEGKFVVHMADQTSWXXXXXXXXXXXXXXXXXXKLYMKGQFPDLPDELKCCL 289
++VT EG +V W ++PD+LKC
Sbjct: 165 ----MLVTPEGGYVKAQPQVQEWKKYKQRTKAVA----------------EIPDQLKCPS 204
Query: 290 TGGLLRDPVKCTKCCGKVVSRLAMEDALLESDFVCPLCQTSDILLDSLEPVQEIQRKVQE 349
LL +P+K + CG + D + D C +C +++ +++ +V++
Sbjct: 205 CHCLLINPMKTS--CGHTLC-----DQCIGMDKKCYVCSR---VIEGFTQDIDMKLEVEK 254
Query: 350 FIKQNK 355
++Q K
Sbjct: 255 ALEQRK 260
Score = 63.5 bits (153), Expect = 6e-10
Identities = 33/78 (42%), Positives = 48/78 (61%), Gaps = 1/78 (1%)
Query: 1 MSSTIYYKFRSQKDTFRVGFDGTGITVFDLKREIINDNKLGDGTDFQLKIYNPDTLEEYE 60
M+S I Y+FRS K+ R+ F GTG+ +++LK EIIN K+ DF L ++ +T EE
Sbjct: 1 MTSVINYRFRSNKNFSRISFQGTGLPLWELKYEIINQRKM-VSKDFDLLFFDGETNEEIN 59
Query: 61 DDTTVIGRSSMVIAKRCP 78
D+ V+ +S VI R P
Sbjct: 60 DEYQVVSMNSHVIVSRIP 77
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.317 0.136 0.403
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 21,322,269
Number of Sequences: 60738
Number of extensions: 908393
Number of successful extensions: 2851
Number of sequences better than 1.0e-05: 8
Number of HSP's better than 0.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 2820
Number of HSP's gapped (non-prelim): 12
length of query: 424
length of database: 30,389,216
effective HSP length: 109
effective length of query: 315
effective length of database: 23,768,774
effective search space: 7487163810
effective search space used: 7487163810
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)