ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3099 good L KOG2671 Replication, recombination and repair Putative RNA methylase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3099 1093564 1092254 -437
(437 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YOL124c [L] KOG2671 Putative RNA methylase 583 e-166
SPBC16D10.02 [L] KOG2671 Putative RNA methylase 248 2e-65
At3g26410 [L] KOG2671 Putative RNA methylase 169 1e-41
7296850 [L] KOG2671 Putative RNA methylase 158 1e-38
Hs11141893 [L] KOG2671 Putative RNA methylase 154 3e-37
CE25550 [L] KOG2671 Putative RNA methylase 145 2e-34
>YOL124c [L] KOG2671 Putative RNA methylase
Length = 433
Score = 583 bits (1503), Expect = e-166
Identities = 294/437 (67%), Positives = 338/437 (77%), Gaps = 8/437 (1%)
Query: 4 KRYLLFMVQVHLNFRLAELESLADLHGXXXXXXXXXXXXPFMIVTLENDKQASDWIRRSV 63
K+YLL+MVQVHLNFR AELESLADL+ PF IV LEND+QA DWI+RS+
Sbjct: 2 KKYLLYMVQVHLNFRRAELESLADLYNLSIDFSQYDANSPFFIVELENDQQAKDWIKRSI 61
Query: 64 LTRAIYEYWGEGNTYESLHKSVQAHPEFEQYKSANERKSXXXXXXX-XXXNAKQDISTRV 122
LTR IYEYWG+G T + LHK +Q FEQ + S N+K + RV
Sbjct: 62 LTRGIYEYWGQGTTLDELHKDIQRQSNFEQDLQLKFKHSTFKFEFECYKGNSK---AKRV 118
Query: 123 TTIESFQYLELKGPIKMKNPDEVFTVIEEYKAISENIGGEVPEKIYFGRLIQLSDRARGA 182
IE+F+YL +G I MK+P EVFTVIEEY ISEN+GG+ P +IYFGR +Q+S+R+ A
Sbjct: 119 EQIETFRYLGFEGKIDMKHPQEVFTVIEEYTPISENVGGKTPTRIYFGRQVQMSNRS--A 176
Query: 183 LEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPAHIMYDPFVGTGSFLVAGGHYGALVLG 242
+EKYDLKKRPYKGTTSFEAELSLVSANIAQVKP IMYDPF GTGSFLVAGGH+G+LV+G
Sbjct: 177 MEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPGTIMYDPFAGTGSFLVAGGHFGSLVIG 236
Query: 243 SDIDGRMIRGKGQQT-IKANFKYYKESIQFLDVMTMDFTHNALRKDFVIDTILCDPPYGI 301
SDIDGRMIRGKG Q I ANFK Y ES QFLDV+TMDFT+NALR + VIDTILCDPPYGI
Sbjct: 237 SDIDGRMIRGKGAQVNISANFKKYGESSQFLDVLTMDFTNNALRNNLVIDTILCDPPYGI 296
Query: 302 RESIKVLGAKDPERFAGKEDVEINGQKAYLLRDYIPTKKPYXXXXXXXXXXXYASQRLPI 361
RESIKVLGAKDPERF GKED+EI+G+KAYL RDYIPTKKPY Y+S+RLPI
Sbjct: 297 RESIKVLGAKDPERFLGKEDMEIDGEKAYLRRDYIPTKKPYALDSLLDDLLQYSSERLPI 356
Query: 362 NGRLAFWMPTANDENVATIIPLHSNLELKYNCVQDFNKWSRRLLVYINRGHEFNGPNNSG 421
GRLAFWMPTAND N+ TI+P+H NLELKYNCVQ+FNKWSRRLLVYINRG FNG +N G
Sbjct: 357 GGRLAFWMPTANDANIETIVPMHENLELKYNCVQEFNKWSRRLLVYINRGSTFNGSSNHG 416
Query: 422 LSRT-GNFRDRYFSGFN 437
+ R+ NFR+RYF+ FN
Sbjct: 417 IKRSKDNFRERYFNNFN 433
>SPBC16D10.02 [L] KOG2671 Putative RNA methylase
Length = 452
Score = 248 bits (632), Expect = 2e-65
Identities = 157/468 (33%), Positives = 230/468 (48%), Gaps = 63/468 (13%)
Query: 6 YLLFMVQVHLNFRLAELESLADLHGX---------------------------------- 31
YLL + H +F L ELE+LA +
Sbjct: 4 YLLHLASTHAHFHLPELETLAKIENVEFWLIPKSEYLNVAFKSDKSRNQALFSHAIKEFQ 63
Query: 32 -XXXXXXXXXXXPFMIVTLENDKQASDWIRRSVLTRAIYEYWGEGNTYESLHKSVQAHPE 90
PFM+ + +++ A WIRRS+ + IYE + G+++ LH+ ++ E
Sbjct: 64 DSISPNDATTQNPFMLAVVNSNEDARRWIRRSIFCKGIYEIYCIGDSFTRLHEKMK---E 120
Query: 91 FEQYKSANERKSXXXXXXXXXXNAKQDISTRVTTIESFQYLELKGPIKMKNPDEVFTVIE 150
+ + + ++ + +++ I F+Y++L+GP+ M NP VFTV+E
Sbjct: 121 LNPAPWDSFKHNSSYKFTFETFGTRRTMKEQLSIISDFEYMQLQGPVSMHNPQHVFTVLE 180
Query: 151 EYKAISENIGGEVPEKIYFGRLIQLSDRARGALEKYDLKKRPYKGTTSFEAELSLVSANI 210
+ N+ G K+YFG +R A++ +DLK+R Y G TSF+AELSLV+A +
Sbjct: 181 NRR---NNVEGP---KVYFGHWC--GSGSRDAIDTFDLKQRSYIGITSFDAELSLVTAQM 232
Query: 211 AQVKPAHIMYDPFVGTGSFLVAGGHYGALVLGSDIDGRMIRGKGQQTIKANFKYYKESIQ 270
A P ++YDPFVGTGSFL +GA LGSDIDGR +RGK ++IK+NF+ YK S
Sbjct: 233 AMAAPGKLIYDPFVGTGSFLYTCSFFGAHTLGSDIDGRQMRGKNGRSIKSNFRQYKLSPF 292
Query: 271 FLDVMTMDFTHNALRKDFVIDTILCDPPYGIRESIKVLGAKDPERFAGKEDVEINGQKAY 330
FLD T D T+ LRK+ ++D I+CDPPYG+R K + AK +R KE
Sbjct: 293 FLDTFTGDVTNCPLRKNLLLDAIVCDPPYGVRAGAKKI-AKCSQR-PPKESSSTG----- 345
Query: 331 LLRDYIPTKKPYXXXXXXXXXXXYASQRLPINGRLAFWMPTANDENVATIIPLHSNLELK 390
++ P + Y +AS RL GRL W+PT +E IP H L L
Sbjct: 346 ---NHYPKLEQYQISDMVYDIICFASDRLVDGGRLVLWLPTITEEYGIDDIPSHPYLSLI 402
Query: 391 YNCVQDFNKWSRRLLVY--INRGHEFNGPN-----NSGLSRTGNFRDR 431
YN +Q F WSRRLL + + R H+ N N+ NFR++
Sbjct: 403 YNSIQPFTHWSRRLLTFQRLPRAHDSKSLNLLPKINNKTPSHHNFREK 450
>At3g26410 [L] KOG2671 Putative RNA methylase
Length = 477
Score = 169 bits (427), Expect = 1e-41
Identities = 126/415 (30%), Positives = 203/415 (48%), Gaps = 35/415 (8%)
Query: 15 LNFRLAELESLADLHGXXXXXXXXXX---------XXPFMIVTLENDKQASDWIRRSVLT 65
L+FR E+E+LA+L G PF V L +++ A + +RS+L
Sbjct: 12 LDFRKPEVEALAELFGEEIAENESLQWRLPENHHNDTPFHFVQLSSEEIARNIAKRSILV 71
Query: 66 RAIYEYWGEGNTYESLHKSVQAHPEFEQY----KSANERKSXXXXXXXXXXNAKQDISTR 121
+ +YE WGEG YE L S+Q++P+ + + R S + ++D
Sbjct: 72 KGMYELWGEGTCYEELKDSIQSYPDSRKLPFLTSDSTFRISVETFGKALTFDEQKD---- 127
Query: 122 VTTIESFQYLELKGPIKMKNPDEVFTVIEEYKAISENIGGE--VPEKIYFGRLIQLSDRA 179
I+SF Y+ +G + +KNPD F ++E ++ EN G + + +I+FGR + +DR
Sbjct: 128 --RIQSFTYIPFEGRVNLKNPDHNFFLMEMIES-EENNGLQPILQRRIFFGREVGFADRK 184
Query: 180 RGALEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPAHIMYDPFVGTGSFLVAGGHYGAL 239
L + LK R Y G T+ +AE++ + AN A+ ++YDPFVGTGS LV+ +GA+
Sbjct: 185 --LLPTFQLKSRTYLGPTAMDAEMAFLMANQAKATSGKLVYDPFVGTGSILVSAARFGAM 242
Query: 240 VLGSDIDGRMIR-GKGQQ-TIKANFKYYKESIQFLDVMTMDFTHNALRKDF--VIDTILC 295
+G+DID R++R G+G + +NFK Y + + ++ MD R + D I+C
Sbjct: 243 TMGADIDIRVVRDGRGPDCNVWSNFKQYGLPMP-VALLRMDNNVPPWRSGLKEIFDAIIC 301
Query: 296 DPPYGIRESIKVLGAKDPERFAGKEDVEINGQKAYLLRDYIPTKKPYXXXXXXXXXXXYA 355
DPPYG+R G K R + V+ D+IP+ Y A
Sbjct: 302 DPPYGVRAG----GRKSGGRKILRGTVDPYTVPDDKRTDHIPSTGAYSLVECVHDLLHLA 357
Query: 356 SQRLPINGRLAFWMPTANDENVATI-IPLHSNLELKYNCVQDF-NKWSRRLLVYI 408
++ L + GRL F+ P DE + + P H +L Q +++SR LL +
Sbjct: 358 ARMLVMKGRLVFFFPVLRDETGSEVKFPEHPCFKLVAVSEQILSSRYSRVLLTMV 412
>7296850 [L] KOG2671 Putative RNA methylase
Length = 460
Score = 158 bits (400), Expect = 1e-38
Identities = 118/413 (28%), Positives = 194/413 (46%), Gaps = 36/413 (8%)
Query: 43 PFMIVTLENDKQASDWIRRSVLTRAIYEYWGEGNTYESLHKSVQAHPEFEQYKSANE-RK 101
PF +V + A + RSV RA++E + N H+ ++ H + A R
Sbjct: 16 PFWLVEFRDTDTALLYASRSVSLRAMFELYAHSNKLPEFHQRLRNHVTANWHSLAEYFRV 75
Query: 102 SXXXXXXXXXXNAKQDISTRVTTIESFQYLELKGPIKMKNPDEVFTVIEEYKAISENIGG 161
N +V IES YL ++G + ++ P + IE + +
Sbjct: 76 DASFKIVVETYNKHFSQREKVEKIESMDYLPIEGTVNLEKPVVEWWYIEFWGLDPTAVPP 135
Query: 162 EVPEKIYFGRLIQLSDRARGALEKYDLKKRPYKGTTSFEAELSLVSANIAQVKPAHIMYD 221
E PE I FGRL ++ R +++ LK R + G TS +A+LSL+ AN A V+ +++D
Sbjct: 136 E-PEDILFGRL--MAHGQRHLIKELSLKHRKFIGNTSMDAQLSLLMANQAMVRNGDLVFD 192
Query: 222 PFVGTGSFLVAGGHYGALVLGSDIDGRMIRGK------------GQQTIKANFKYYKESI 269
PFVGTGS LV+ +G VLG+DID M+ + ++I+AN K Y +
Sbjct: 193 PFVGTGSLLVSAAKWGGYVLGADIDYMMVHARCRPSRITQKVREKDESIRANLKQYGCAD 252
Query: 270 QFLDVMTMDFTHNALRKDFVIDTILCDPPYGIRESIKVLGAKDPERFAGKEDVEINGQKA 329
+++DV+ DF++ D I+ DPPYGIRE+ E+ K V+ N +
Sbjct: 253 RYMDVVVADFSNPVWHSRISFDCIITDPPYGIREA--------TEKVEKKASVKANTRSD 304
Query: 330 YLLRDYIPTKKPYXXXXXXXXXXXYASQRLPINGRLAFWMPTANDENVATIIPLHSNLEL 389
++ + P+ Y ++++ L + GRL W+P +++ ++P H +L L
Sbjct: 305 SMV--HYPSTSHYSLQSLYGDLLEFSARHLKLGGRLVCWIPFHSEDYDPKMLPQHQHLSL 362
Query: 390 KYNCVQDF-NKWSRRLLVYINRGHEFNGPNNSGL-------SRTGNFRDRYFS 434
N Q +RRLL Y + E++ P L + + +FRDRYF+
Sbjct: 363 IANSEQQLTGNTARRLLTY-EKCFEYS-PTEPLLCSIKQVPTPSQDFRDRYFN 413
>Hs11141893 [L] KOG2671 Putative RNA methylase
Length = 257
Score = 154 bits (388), Expect = 3e-37
Identities = 87/249 (34%), Positives = 136/249 (53%), Gaps = 6/249 (2%)
Query: 5 RYLLFMVQVHLNFRLAELESLADLHGXXXXXXXXXXX-XPFMIVTLENDKQASDWIRRSV 63
RYLL M Q HL FRL E++SL L G PF I+++ ++ A + ++R+V
Sbjct: 9 RYLLLMAQEHLEFRLPEIKSLLLLFGGQFASSQETYGKSPFWILSIPSEDIARNLMKRTV 68
Query: 64 LTRAIYEYWGEGNTYESLHKSVQAHPEFEQYKSANERKSXXXXXXXXXXNAKQDISTRVT 123
++I+E WG G + E L+ S++ +P + + + Q+ ++
Sbjct: 69 CAKSIFELWGHGQSPEELYSSLKNYPVEKMVPFLHSDSTYKIKIHTFNKTLTQE--EKIK 126
Query: 124 TIESFQYLELKGPIKMKNPDEVFTVIEEYKAISENIGGEVPEKIYFGRLIQLSDRARGAL 183
I++ ++L +G + +K P VF+V+E+Y + N E P IYFGR I +D R +
Sbjct: 127 RIDALEFLPFEGKVNLKKPQHVFSVLEDY-GLDPNCIPENPHNIYFGRWI--ADGQRELI 183
Query: 184 EKYDLKKRPYKGTTSFEAELSLVSANIAQVKPAHIMYDPFVGTGSFLVAGGHYGALVLGS 243
E Y +KKR + G TS +A LS + AN +VK I++DPFVGTG L+A H+GA V G+
Sbjct: 184 ESYSVKKRHFIGNTSMDAGLSFIMANHGKVKENDIVFDPFVGTGGLLIACAHFGAYVYGT 243
Query: 244 DIDGRMIRG 252
DID + G
Sbjct: 244 DIDYNTVHG 252
>CE25550 [L] KOG2671 Putative RNA methylase
Length = 323
Score = 145 bits (365), Expect = 2e-34
Identities = 96/316 (30%), Positives = 153/316 (48%), Gaps = 26/316 (8%)
Query: 7 LLFMVQVHLNFRLAELESLADLHGXXXXXXXXXXXXPFM-IVTLENDKQASDWIRRSVLT 65
L+ Q H++FR+AE E++ + M IV E+ + + RS+L
Sbjct: 5 LVIFSQSHVDFRIAEFEAICSMFSIKVDLSEKLNTTDHMFIVNFESHLEVRKLLSRSILV 64
Query: 66 RAIYEYWGEGNTYESLHKSVQAHPEF-EQYKSANERKSXXXXXXXXXXNAKQDISTRVTT 124
+ E E +Y+ +++ + PE E++ E S K D R+
Sbjct: 65 KYAIELSIEAESYDIMYEQIDNKPEIIEKFDKLEE--SFAIRFFAIGRKKKLDSIERIKA 122
Query: 125 IESFQYLELKGPIKMKNPDEVFTVIEEYKAISENIGGEVPEKIYFGRLIQLSDRARGALE 184
K PI +K+P ++EEY EN E P+K+YFG+LI R L+
Sbjct: 123 FLDVVPFN-KAPICLKSPKNELFLVEEY----ENPSDEAPKKVYFGKLI---GEGRAELK 174
Query: 185 -KYDLKKRPYKGTTSFEAELSLVSANIAQVKPAHIMYDPFVGTGSFLVAGGHYGALVLGS 243
KY+L+ R Y G T+ + ELS + +N+A + P I+ DPFVGTG ++ +GA V+G+
Sbjct: 175 TKYNLRDRCYIGNTTMDPELSFIQSNLAMISPGDIVLDPFVGTGGLILPAAEFGAFVIGT 234
Query: 244 DIDGRMIRGKGQ------------QTIKANFKYYKESIQFLDVMTMDFT-HNALRKDFVI 290
+I+ + R +G+ ++IKANF+ YK QFL V+ D + H +
Sbjct: 235 EINYQTARAQGRSSRQGVGQRGESESIKANFEQYKSESQFLGVLIADSSKHGIWSCNAKF 294
Query: 291 DTILCDPPYGIRESIK 306
D I+ DPPYG+RE +
Sbjct: 295 DAIVADPPYGVREKAR 310
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.319 0.138 0.404
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 24,573,320
Number of Sequences: 60738
Number of extensions: 984432
Number of successful extensions: 1887
Number of sequences better than 1.0e-05: 6
Number of HSP's better than 0.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 1855
Number of HSP's gapped (non-prelim): 7
length of query: 437
length of database: 30,389,216
effective HSP length: 109
effective length of query: 328
effective length of database: 23,768,774
effective search space: 7796157872
effective search space used: 7796157872
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)