ORF STATUS Function Best COG Functional category Pathways and functional systems
r_klactIII3174 good CO KOG3996 Energy production and conversion Holocytochrome c synthase/heme-lyase
r_klactIII3174 good CO KOG3996 Posttranslational modification, protein turnover, chaperones Holocytochrome c synthase/heme-lyase
Only best alignment is shown:
BLASTP 2.2.3 [May-13-2002]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= r_klactIII3174 1118969 1118010 -320
(320 letters)
Database: KOG eukaryal database 04/03
60,738 sequences; 30,389,216 total letters
Searching..................................................done
Color Key for Alignment Scores: 
Score E
Sequences producing significant alignments: (bits) Value
YAL039c [CO] KOG3996 Holocytochrome c synthase/heme-lyase 311 6e-85
SPBC26H8.12 [CO] KOG3996 Holocytochrome c synthase/heme-lyase 164 2e-40
CE02327 [CO] KOG3996 Holocytochrome c synthase/heme-lyase 140 3e-33
7300803 [CO] KOG3996 Holocytochrome c synthase/heme-lyase 136 5e-32
Hs4885401 [CO] KOG3996 Holocytochrome c synthase/heme-lyase 124 2e-28
SPAC24C9.02c [CO] KOG3996 Holocytochrome c synthase/heme-lyase 117 3e-26
YKL087c [CO] KOG3996 Holocytochrome c synthase/heme-lyase 86 7e-17
>YAL039c [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 269
Score = 311 bits (798), Expect = 6e-85
Identities = 157/279 (56%), Positives = 198/279 (70%), Gaps = 24/279 (8%)
Query: 28 MGWFWADKLSTASTNATPISGDISACPVMHDKPKGSDVSACPVLHDKKTTDKPTGTEGGC 87
MGWFWAD+ +T DI V S +S CPV+H+ ++ P+ C
Sbjct: 1 MGWFWADQKTTGK--------DIGGAAV-------SSMSGCPVMHESSSSSPPSSE---C 42
Query: 88 PVLSDQGNHGLNPLNNIPIALTDVKQSGQKLDLPTERTVSSIPKGKTADDEFWEYPSPQQ 147
PV+ N +NPLNN+P L KQ GQK+DLP +RT+SSIPK ++ EFWEYPSPQQ
Sbjct: 43 PVMQGD-NDRINPLNNMP-ELAASKQPGQKMDLPVDRTISSIPKSPDSN-EFWEYPSPQQ 99
Query: 148 MYNAMVRKGKIDPFTGEEIPEDAVESMVFVHNFLNEGCWQEILDWEKKYTDKTHTYPKLL 207
MYNAMVRKGKI E+ EDAVESMV VHNFLNEGCWQE+L+WEK +TD++H PKLL
Sbjct: 100 MYNAMVRKGKIGG--SGEVAEDAVESMVQVHNFLNEGCWQEVLEWEKPHTDESHVQPKLL 157
Query: 208 QFMGKPDQLSPRARCFNIFGTLFPGYFSSELPFDRHDWIVLRPDPTSSDTENPGYRKVRY 267
+FMGKP LSPRAR ++ G LFP +FS ELPFDRHDWIVLR + ++ + P +++VRY
Sbjct: 158 KFMGKPGVLSPRARWMHLCGLLFPSHFSQELPFDRHDWIVLRGE-RKAEQQPPTFKEVRY 216
Query: 268 IIDFYGGPDDEEGLPTFNLDVRPALDNFGNAKDRFERWM 306
++DFYGGPDDE G+PTF++DVRPALD+ NAKDR R++
Sbjct: 217 VLDFYGGPDDENGMPTFHVDVRPALDSLDNAKDRMTRFL 255
>SPBC26H8.12 [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 377
Score = 164 bits (415), Expect = 2e-40
Identities = 112/288 (38%), Positives = 148/288 (50%), Gaps = 49/288 (17%)
Query: 40 STNATPISGDISACPVMHDKPKGSDVSACPVLHDKKTTDKPTGTEGGCPVLSDQ------ 93
++ ATP + CP+ + K + VS D K + + GCPV + +
Sbjct: 112 NSEATPAVQPPATCPMSNSNQKPAGVSEVLTGVDSK---QQSYVPEGCPVATPKRGWFNW 168
Query: 94 -GNHGLNP------------LNNIPIALTDVKQSGQKLDLPTERTVSSIPKGKTADDEFW 140
GN+ NIP D Q + L T RT SSIPK D + W
Sbjct: 169 FGNNDDQKQEAYEVDKSNMMYKNIPQTAVD----DQVVGLETTRTTSSIPK---VDGKNW 221
Query: 141 EYPSPQQMYNAMVRKGKIDPFTGEEIPEDAVESMVFVHNFLNEGCWQEILDWEKKYTDKT 200
EYPSPQQMYNAM RKG D +GE +P MV VHNFLNEG W EI WE++ + T
Sbjct: 222 EYPSPQQMYNAMWRKGYRD--SGENVP-----IMVQVHNFLNEGAWSEIKAWEREAGENT 274
Query: 201 HTYPKLLQFMGKPDQLSPRARCFNIFGTLFPGYFSS-ELPFDRHDWIVLRPDPTSSDTEN 259
PKLL+F G ++ +PRA + + G + P + S E PFDRHDW V R D +
Sbjct: 275 E--PKLLRFEGNANKRTPRALWYMMLGRINPNRWGSGEGPFDRHDWYVQRKDNSI----- 327
Query: 260 PGYRKVRYIIDFYGGPDDEEGLPTFNLDVRPALDNFGNAKDRFERWMA 307
VRY+ID+Y PD +G P F+LDVRPA+D+F + R++ W A
Sbjct: 328 -----VRYVIDYYEAPDSADGKPVFSLDVRPAVDSFESVALRWKHWRA 370
>CE02327 [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 280
Score = 140 bits (352), Expect = 3e-33
Identities = 97/270 (35%), Positives = 138/270 (50%), Gaps = 31/270 (11%)
Query: 40 STNATPISGDISACP-----VMHDKPKGSDVSACPVLHDKKT--TDKPTGTEGGCPVLSD 92
S+ +TP D +A H S CP+ +++ + + G G CPV +D
Sbjct: 3 SSQSTPKVQDANADAERIRKAQHSMAAAGGGSQCPLTPEQRAAASGENCGAGGACPVGAD 62
Query: 93 QGNHGLNPLNNIPIALTDVKQSGQKLDLPTERTVSSIPKGKTADDEFWEYPSPQQMYNAM 152
+ + +NPLNN Q LPT+R S+IPK T + E W YPSPQ +NAM
Sbjct: 63 KAS--INPLNNELEHPNQKPAPDQPFALPTKREKSTIPKAGT-ETETWTYPSPQMFWNAM 119
Query: 153 VRKGKIDPFTGEEIPEDAVESMVFVHNFLNEGCWQEILDWEKKYTDKTHTYPKLLQFMGK 212
++KG + + + + +E+++ +HN NE W+E+L WE + PKL F G
Sbjct: 120 LKKGW--RWQDDSLSKSDMENIISIHNANNEEAWREVLKWENLLHPEC-AEPKLKSFKGD 176
Query: 213 PDQLSPRARCFNIFGTLFPGYFSSELPFDRHDWIVLRPDPTSSDTENPGYRKVRYIIDFY 272
LSPRAR F LF GY +LPFDRHDWIV + G ++V+Y+ID+Y
Sbjct: 177 AKNLSPRAR----FRNLFLGY---DLPFDRHDWIV----------DRCGTKQVQYVIDYY 219
Query: 273 -GGPDDEEGLPTFNLDVRPALDNFGNAKDR 301
GG D LDVRPA+++ GN DR
Sbjct: 220 DGGAVDPSSKLFTILDVRPAVNDIGNIWDR 249
>7300803 [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 281
Score = 136 bits (342), Expect = 5e-32
Identities = 94/271 (34%), Positives = 127/271 (46%), Gaps = 49/271 (18%)
Query: 48 GDISACPVMHDKPKGSDVSACPVLHDKKTTDKPTGTEGGCPVLSDQGNHGLNPLNNIPIA 107
GD + + PK S CPV HD N +NPLN +P A
Sbjct: 42 GDAKSASAVPPHPKMQAASECPVQHD---------------------NSDVNPLNMMPPA 80
Query: 108 LTDVKQSGQKLDLPTERTVSSIPK-GKTADDEFWEYPSPQQMYNAMVRKGKIDPFTGEEI 166
+ Q LPT+R S+IPK + +FW+YPS Q +NAM+RKG + E++
Sbjct: 81 -NQQPAADQPFPLPTDRQTSTIPKVTEDGSVQFWQYPSQQMFWNAMLRKGW--RWKTEDV 137
Query: 167 PEDAVESMVFVHNFLNEGCWQEILDWEKKYTDKTHTYPKLLQFMGKPDQLSPRARCFNIF 226
+ + ++ +HN NE WQE+L WE + K P+L F GK SPRAR
Sbjct: 138 SQKDMGDIIRIHNANNEQAWQEVLKWEALHA-KECGNPRLKSFGGKAKDFSPRAR----- 191
Query: 227 GTLFPGYFSSELPFDRHDWIVLRPDPTSSDTENPGYRKVRYIIDFYGGPDDEEGLPTFNL 286
F + ELPFDRHDWIV R + VRY+ID+Y G ++ L
Sbjct: 192 ---FRSWLGYELPFDRHDWIVDRCG-----------KDVRYVIDYYDGGLVDKDYRFALL 237
Query: 287 DVRPALDNFGNAKDR----FERWMAPTLEKY 313
DVRPA+D+ N DR + RW EK+
Sbjct: 238 DVRPAMDSVDNVWDRMRVAYMRWKYELFEKF 268
>Hs4885401 [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 268
Score = 124 bits (311), Expect = 2e-28
Identities = 94/283 (33%), Positives = 136/283 (47%), Gaps = 41/283 (14%)
Query: 36 LSTASTNATPISGDISACPVMHDKPKGSDVSACPV--LHDKKTTDKPTGTEGG-----CP 88
+ ++ +A+P SG CP+ K KG V+ P +KKT P E CP
Sbjct: 12 VQASNASASPPSG----CPMHEGKMKGCPVNTEPSGPTCEKKTYSVPAHQERAYEYVECP 67
Query: 89 V--LSDQGNHGLNPLNNIPIALTDVKQSGQKLDLPTERTVSSIPKGKTADDEFWEYPSPQ 146
+ + + L+P N +P Q L T R SSIP+ + ++ W YPS Q
Sbjct: 68 IRGTAAENKENLDPSNLMPPP-NQTPAPDQPFALSTVREESSIPRADS--EKKWVYPSEQ 124
Query: 147 QMYNAMVRKGKIDPFTGEEIPEDAVESMVFVHNFLNEGCWQEILDWEKKYTDKTHTYPKL 206
+NAM++KG + E+I + + +++ +HN NE W+EIL WE + + P L
Sbjct: 125 MFWNAMLKKGW--KWKDEDISQKDMYNIIRIHNQNNEQAWKEILKWEALHAAECPCGPSL 182
Query: 207 LQFMGKPDQLSPRARCFNIFGTLFPGYFSSELPFDRHDWIVLRPDPTSSDTENPGYRKVR 266
++F GK + SPRAR + ELPFDRHDWI+ R TE VR
Sbjct: 183 IRFGGKAKEYSPRAR--------IRSWMGYELPFDRHDWIINR-----CGTE------VR 223
Query: 267 YIIDFYGGPDDEEGLPTFNLDVRPALDNFGNAKDRFE----RW 305
Y+ID+Y G + + LDVRPALD+ DR + RW
Sbjct: 224 YVIDYYDGGEVNKDYQFTILDVRPALDSLSAVWDRMKVAWWRW 266
>SPAC24C9.02c [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 216
Score = 117 bits (292), Expect = 3e-26
Identities = 78/190 (41%), Positives = 100/190 (52%), Gaps = 32/190 (16%)
Query: 120 LPTERTVSSIPKGKTADD----EFWEYPSPQQMYNAMVRKGKIDPFTGEEIPEDAVESMV 175
LPTER +S+IPK T D E W YPS Q ++AM RK PED ++++V
Sbjct: 45 LPTEREISTIPKVVTESDSGKEEKWIYPSQQMFFDAMKRKN------WNPHPED-MKTIV 97
Query: 176 FVHNFLNEGCWQEILDWEKKYTDKTHTYPKLLQFMGKPDQLSPRARCFNIFGTLFPGYFS 235
+HN +NE WQ+IL WE+ + + PKL +F G +L+P+AR N+ G
Sbjct: 98 PIHNAVNERAWQDILQWEQGWGSEKCGGPKLERFDGNVKKLTPKARILNLLG-------- 149
Query: 236 SELPFDRHDWIVLRPDPTSSDTENPGYRKVRYIIDFYGGPDDEEGLPTFNLDVRPALDNF 295
PFDRHDW+V N RKV Y+IDFY GP G P+ LDVRP L
Sbjct: 150 YNKPFDRHDWLV-----------NRCGRKVAYVIDFYNGP-TVNGTPSIYLDVRPKLSVH 197
Query: 296 GNAKDRFERW 305
G A R RW
Sbjct: 198 G-AWMRVYRW 206
>YKL087c [CO] KOG3996 Holocytochrome c synthase/heme-lyase
Length = 224
Score = 85.9 bits (211), Expect = 7e-17
Identities = 78/246 (31%), Positives = 107/246 (42%), Gaps = 48/246 (19%)
Query: 68 CPVLHD-KKTTDKPTGTEG--GCPVLSDQGNHGLNPLNNIPIALTDVKQSGQKLDLPTER 124
CPV + KK + G E G +Q NP +N + +DL R
Sbjct: 10 CPVDEETKKLWLREHGNEAHPGATAPGNQLECSANPQDN-----DKTPEYHTTVDLSQSR 64
Query: 125 TVSSIPKGKTADDEFWEYPSPQQMYNAMVRKGKIDPFTGEEIPEDAVESMVFVHNFLNEG 184
VS+IP+ T D W YPS +Q Y AM++K DP + D ++ +V +HN +NE
Sbjct: 65 EVSTIPR--TNSDRNWIYPSEKQFYEAMMKKNW-DPNS------DDMKVVVPLHNSINER 115
Query: 185 CWQEILDWEKKYTDKTHTYPKLLQFMGKPDQLSPRARCFNIFGTLFPGYFSSEL-----P 239
W I WE K + KL F G +L+PRA +F S + P
Sbjct: 116 VWNYIKSWEDKQGGEACGGIKLTNFKGDSKKLTPRA------------WFRSRILHLAKP 163
Query: 240 FDRHDWIVLRPDPTSSDTENPGYRKVRYIIDFYG---GPDDEEGLPTFNLDVRPALDNFG 296
FDRHDW + R T V Y+IDFY + + P LDVRP L++F
Sbjct: 164 FDRHDWQIDRCGKT-----------VDYVIDFYSTDLNDANSQQQPLIYLDVRPKLNSFE 212
Query: 297 NAKDRF 302
+ RF
Sbjct: 213 GFRLRF 218
Database: KOG eukaryal database 04/03
Posted date: Apr 14, 2003 1:07 PM
Number of letters in database: 30,389,216
Number of sequences in database: 60,738
Lambda K H
0.316 0.136 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 22,503,446
Number of Sequences: 60738
Number of extensions: 1112317
Number of successful extensions: 2330
Number of sequences better than 1.0e-05: 7
Number of HSP's better than 0.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2291
Number of HSP's gapped (non-prelim): 9
length of query: 320
length of database: 30,389,216
effective HSP length: 106
effective length of query: 214
effective length of database: 23,950,988
effective search space: 5125511432
effective search space used: 5125511432
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)